This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 62.73 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,442,494 Reads with adapters: 55,220 (2.3%) Reads that were too short: 17,975 (0.7%) Reads written (passing filters): 37,245 (1.5%) Total basepairs processed: 735,190,694 bp Total written (filtered): 6,008,192 bp (0.8%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 55220 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 178 596.3 0 178 7 988 596.3 0 988 8 19 596.3 0 19 9 868 596.3 0 868 10 126 596.3 0 126 11 13 596.3 0 13 12 16 596.3 0 16 13 9 596.3 0 9 14 19 596.3 0 19 15 10 596.3 0 10 16 12 596.3 0 12 17 13 596.3 0 13 18 7 596.3 0 7 19 11 596.3 0 11 20 15 596.3 0 15 21 18 596.3 0 18 22 12 596.3 0 12 23 9 596.3 0 9 24 5 596.3 0 5 25 13 596.3 0 13 26 9 596.3 0 9 27 12 596.3 0 12 28 16 596.3 0 16 29 10 596.3 0 10 30 13 596.3 0 13 31 12 596.3 0 12 32 14 596.3 0 14 33 18 596.3 0 18 34 25 596.3 0 25 35 24 596.3 0 24 36 19 596.3 0 19 37 16 596.3 0 16 38 76 596.3 0 76 39 56 596.3 0 56 40 92 596.3 0 92 41 12 596.3 0 12 42 11 596.3 0 11 43 16 596.3 0 16 44 11 596.3 0 11 45 16 596.3 0 16 46 18 596.3 0 18 47 9 596.3 0 9 48 8 596.3 0 8 49 22 596.3 0 22 50 14 596.3 0 14 51 15 596.3 0 15 52 24 596.3 0 24 53 138 596.3 0 138 54 79 596.3 0 79 55 126 596.3 0 126 56 29 596.3 0 29 57 203 596.3 0 203 58 18 596.3 0 18 59 16 596.3 0 16 60 26 596.3 0 26 61 21 596.3 0 21 62 10 596.3 0 10 63 14 596.3 0 14 64 16 596.3 0 16 65 24 596.3 0 24 66 63 596.3 0 63 67 17 596.3 0 17 68 39 596.3 0 39 69 135 596.3 0 135 70 25 596.3 0 25 71 50 596.3 0 50 72 27 596.3 0 27 73 19 596.3 0 19 74 14 596.3 0 14 75 18 596.3 0 18 76 13 596.3 0 13 77 16 596.3 0 16 78 8 596.3 0 8 79 7 596.3 0 7 80 14 596.3 0 14 81 17 596.3 0 17 82 18 596.3 0 18 83 26 596.3 0 26 84 12 596.3 0 12 85 17 596.3 0 17 86 18 596.3 0 18 87 26 596.3 0 26 88 23 596.3 0 23 89 31 596.3 0 31 90 28 596.3 0 28 91 57 596.3 0 57 92 27 596.3 0 27 93 40 596.3 0 40 94 39 596.3 0 39 95 34 596.3 0 34 96 24 596.3 0 24 97 18 596.3 0 18 98 25 596.3 0 25 99 13 596.3 0 13 100 16 596.3 0 16 101 27 596.3 0 27 102 26 596.3 0 26 103 51 596.3 0 51 104 70 596.3 0 70 105 65 596.3 0 65 106 36 596.3 0 36 107 35 596.3 0 35 108 32 596.3 0 32 109 37 596.3 0 37 110 38 596.3 0 38 111 45 596.3 0 45 112 37 596.3 0 37 113 83 596.3 0 83 114 27 596.3 0 27 115 48 596.3 0 48 116 26 596.3 0 26 117 51 596.3 0 51 118 30 596.3 0 30 119 45 596.3 0 45 120 54 596.3 0 54 121 58 596.3 0 58 122 53 596.3 0 53 123 96 596.3 0 96 124 174 596.3 0 174 125 2092 596.3 0 2092 126 1104 596.3 0 1104 127 2772 596.3 0 2772 128 72 596.3 0 72 129 37 596.3 0 37 130 35 596.3 0 35 131 42 596.3 0 42 132 67 596.3 0 67 133 70 596.3 0 70 134 50 596.3 0 50 135 44 596.3 0 44 136 19 596.3 0 19 137 33 596.3 0 33 138 26 596.3 0 26 139 40 596.3 0 40 140 51 596.3 0 51 141 29 596.3 0 29 142 54 596.3 0 54 143 80 596.3 0 80 144 105 596.3 0 105 145 283 596.3 0 283 146 189 596.3 0 189 147 158 596.3 0 158 148 77 596.3 0 77 149 95 596.3 0 95 150 303 596.3 0 303 151 188 596.3 0 188 152 293 596.3 0 293 153 83 596.3 0 83 154 180 596.3 0 180 155 239 596.3 0 239 156 571 596.3 0 571 157 564 596.3 0 564 158 382 596.3 0 382 159 471 596.3 0 471 160 4895 596.3 0 4895 161 4392 596.3 0 4392 162 5284 596.3 0 5284 163 121 596.3 0 121 164 94 596.3 0 94 165 58 596.3 0 58 166 79 596.3 0 79 167 64 596.3 0 64 168 67 596.3 0 67 169 462 596.3 0 462 170 67 596.3 0 67 171 236 596.3 0 236 172 41 596.3 0 41 173 68 596.3 0 68 174 57 596.3 0 57 175 252 596.3 0 252 176 276 596.3 0 276 177 268 596.3 0 268 178 58 596.3 0 58 179 85 596.3 0 85 180 416 596.3 0 416 181 812 596.3 0 812 182 942 596.3 0 942 183 454 596.3 0 454 184 56 596.3 0 56 185 64 596.3 0 64 186 78 596.3 0 78 187 75 596.3 0 75 188 59 596.3 0 59 189 40 596.3 0 40 190 41 596.3 0 41 191 52 596.3 0 52 192 37 596.3 0 37 193 31 596.3 0 31 194 39 596.3 0 39 195 44 596.3 0 44 196 40 596.3 0 40 197 48 596.3 0 48 198 46 596.3 0 46 199 55 596.3 0 55 200 53 596.3 0 53 201 54 596.3 0 54 202 38 596.3 0 38 203 55 596.3 0 55 204 42 596.3 0 42 205 45 596.3 0 45 206 32 596.3 0 32 207 57 596.3 0 57 208 76 596.3 0 76 209 58 596.3 0 58 210 99 596.3 0 99 211 306 596.3 0 306 212 1312 596.3 0 1312 213 1603 596.3 0 1603 214 2831 596.3 0 2831 215 73 596.3 0 73 216 44 596.3 0 44 217 52 596.3 0 52 218 62 596.3 0 62 219 87 596.3 0 87 220 52 596.3 0 52 221 54 596.3 0 54 222 47 596.3 0 47 223 42 596.3 0 42 224 67 596.3 0 67 225 105 596.3 0 105 226 104 596.3 0 104 227 51 596.3 0 51 228 53 596.3 0 53 229 64 596.3 0 64 230 48 596.3 0 48 231 37 596.3 0 37 232 31 596.3 0 31 233 39 596.3 0 39 234 61 596.3 0 61 235 60 596.3 0 60 236 65 596.3 0 65 237 63 596.3 0 63 238 106 596.3 0 106 239 143 596.3 0 143 240 102 596.3 0 102 241 103 596.3 0 103 242 50 596.3 0 50 243 112 596.3 0 112 244 66 596.3 0 66 245 74 596.3 0 74 246 93 596.3 0 93 247 112 596.3 0 112 248 113 596.3 0 113 249 67 596.3 0 67 250 49 596.3 0 49 251 48 596.3 0 48 252 90 596.3 0 90 253 60 596.3 0 60 254 81 596.3 0 81 255 157 596.3 0 157 256 494 596.3 0 494 257 261 596.3 0 261 258 550 596.3 0 550 259 156 596.3 0 156 260 137 596.3 0 137 261 86 596.3 0 86 262 77 596.3 0 77 263 78 596.3 0 78 264 72 596.3 0 72 265 69 596.3 0 69 266 177 596.3 0 177 267 236 596.3 0 236 268 357 596.3 0 357 269 139 596.3 0 139 270 97 596.3 0 97 271 115 596.3 0 115 272 283 596.3 0 283 273 532 596.3 0 532 274 650 596.3 0 650 275 605 596.3 0 605 276 142 596.3 0 142 277 194 596.3 0 194 278 293 596.3 0 293 279 136 596.3 0 136 280 170 596.3 0 170 281 129 596.3 0 129 282 121 596.3 0 121 283 84 596.3 0 84 284 118 596.3 0 118 285 83 596.3 0 83 286 104 596.3 0 104 287 237 596.3 0 237 288 159 596.3 0 159 289 192 596.3 0 192 290 66 596.3 0 66 291 55 596.3 0 55 292 92 596.3 0 92 293 159 596.3 0 159 294 130 596.3 0 130 295 102 596.3 0 102 296 78 596.3 0 78 297 88 596.3 0 88 298 65 596.3 0 65 299 66 596.3 0 66 300 71 596.3 0 71 301 129 596.3 0 129 Finished in 1.34 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 37,245 Reads with adapters: 1,225 (3.3%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 1,215 (3.3%) Total basepairs processed: 6,008,192 bp Total written (filtered): 321,291 bp (5.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1225 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 58 0.0 3 0 0 0 0 58 19 17 0.0 3 0 17 20 985 0.0 4 960 25 21 5 0.0 4 1 3 1 22 4 0.0 4 3 1 23 122 0.0 4 121 0 0 1 26 1 0.0 4 0 0 0 1 28 1 0.0 4 1 39 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 60 4 0.0 4 0 0 0 0 4 61 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 73 1 0.0 4 1 101 1 0.0 4 0 0 0 1 103 2 0.0 4 0 0 1 1 105 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 13 0.0 4 0 0 0 0 13 129 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.04 s (33 us/read; 1.82 M reads/minute). === Summary === Total reads processed: 1,215 Reads with adapters: 58 (4.8%) Reads written (passing filters): 1,215 (100.0%) Total basepairs processed: 321,291 bp Total written (filtered): 320,386 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 58 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.7% C: 10.3% G: 86.2% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 19.0 0 2 9 4 0.0 0 0 4 10 10 0.0 1 2 8 11 37 0.0 1 0 37 12 3 0.0 1 0 3 49 1 0.0 1 1 271 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.