This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.63 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 50,370 (2.0%) Reads that were too short: 10,770 (0.4%) Reads written (passing filters): 39,600 (1.6%) Total basepairs processed: 740,966,583 bp Total written (filtered): 10,203,813 bp (1.4%) === Adapter 1 === Sequence: GTGATC; Type: regular 5'; Length: 6; Trimmed: 50370 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 244 601.0 0 244 7 329 601.0 0 329 8 14790 601.0 0 14790 9 11388 601.0 0 11388 10 53 601.0 0 53 11 2 601.0 0 2 13 3 601.0 0 3 14 3 601.0 0 3 15 2 601.0 0 2 16 1 601.0 0 1 18 3 601.0 0 3 19 8 601.0 0 8 20 3 601.0 0 3 21 13 601.0 0 13 22 6 601.0 0 6 23 13 601.0 0 13 24 17 601.0 0 17 25 18 601.0 0 18 26 5 601.0 0 5 27 5 601.0 0 5 28 4 601.0 0 4 29 14 601.0 0 14 30 12 601.0 0 12 31 7 601.0 0 7 32 18 601.0 0 18 33 42 601.0 0 42 34 23 601.0 0 23 35 109 601.0 0 109 36 22 601.0 0 22 37 69 601.0 0 69 38 43 601.0 0 43 39 21 601.0 0 21 40 15 601.0 0 15 41 9 601.0 0 9 42 3 601.0 0 3 43 9 601.0 0 9 44 8 601.0 0 8 45 10 601.0 0 10 46 8 601.0 0 8 47 9 601.0 0 9 48 5 601.0 0 5 49 5 601.0 0 5 50 10 601.0 0 10 51 14 601.0 0 14 52 9 601.0 0 9 53 14 601.0 0 14 54 15 601.0 0 15 55 10 601.0 0 10 56 6 601.0 0 6 57 16 601.0 0 16 58 69 601.0 0 69 59 90 601.0 0 90 60 154 601.0 0 154 61 9 601.0 0 9 62 14 601.0 0 14 63 19 601.0 0 19 64 8 601.0 0 8 65 6 601.0 0 6 66 17 601.0 0 17 67 11 601.0 0 11 68 22 601.0 0 22 69 16 601.0 0 16 70 17 601.0 0 17 71 35 601.0 0 35 72 10 601.0 0 10 73 95 601.0 0 95 74 19 601.0 0 19 75 30 601.0 0 30 76 231 601.0 0 231 77 222 601.0 0 222 78 573 601.0 0 573 79 16 601.0 0 16 80 268 601.0 0 268 81 220 601.0 0 220 82 365 601.0 0 365 83 19 601.0 0 19 84 8 601.0 0 8 85 19 601.0 0 19 86 10 601.0 0 10 87 14 601.0 0 14 88 12 601.0 0 12 89 14 601.0 0 14 90 18 601.0 0 18 91 12 601.0 0 12 92 4 601.0 0 4 93 12 601.0 0 12 94 20 601.0 0 20 95 18 601.0 0 18 96 49 601.0 0 49 97 15 601.0 0 15 98 14 601.0 0 14 99 40 601.0 0 40 100 390 601.0 0 390 101 1351 601.0 0 1351 102 2156 601.0 0 2156 103 9 601.0 0 9 104 19 601.0 0 19 105 11 601.0 0 11 106 8 601.0 0 8 107 13 601.0 0 13 108 11 601.0 0 11 109 48 601.0 0 48 110 266 601.0 0 266 111 325 601.0 0 325 112 306 601.0 0 306 113 24 601.0 0 24 114 10 601.0 0 10 115 14 601.0 0 14 116 7 601.0 0 7 117 38 601.0 0 38 118 12 601.0 0 12 119 9 601.0 0 9 120 17 601.0 0 17 121 5 601.0 0 5 122 10 601.0 0 10 123 12 601.0 0 12 124 14 601.0 0 14 125 9 601.0 0 9 126 8 601.0 0 8 127 99 601.0 0 99 128 21 601.0 0 21 129 17 601.0 0 17 130 89 601.0 0 89 131 27 601.0 0 27 132 29 601.0 0 29 133 22 601.0 0 22 134 9 601.0 0 9 135 13 601.0 0 13 136 49 601.0 0 49 137 6 601.0 0 6 138 23 601.0 0 23 139 11 601.0 0 11 140 14 601.0 0 14 141 8 601.0 0 8 142 36 601.0 0 36 143 26 601.0 0 26 144 16 601.0 0 16 145 21 601.0 0 21 146 25 601.0 0 25 147 80 601.0 0 80 148 57 601.0 0 57 149 186 601.0 0 186 150 110 601.0 0 110 151 95 601.0 0 95 152 410 601.0 0 410 153 22 601.0 0 22 154 10 601.0 0 10 155 28 601.0 0 28 156 17 601.0 0 17 157 36 601.0 0 36 158 52 601.0 0 52 159 23 601.0 0 23 160 15 601.0 0 15 161 16 601.0 0 16 162 49 601.0 0 49 163 11 601.0 0 11 164 13 601.0 0 13 165 14 601.0 0 14 166 14 601.0 0 14 167 13 601.0 0 13 168 56 601.0 0 56 169 73 601.0 0 73 170 201 601.0 0 201 171 11 601.0 0 11 172 5 601.0 0 5 173 6 601.0 0 6 174 6 601.0 0 6 175 14 601.0 0 14 176 18 601.0 0 18 177 48 601.0 0 48 178 37 601.0 0 37 179 32 601.0 0 32 180 22 601.0 0 22 181 25 601.0 0 25 182 105 601.0 0 105 183 183 601.0 0 183 184 59 601.0 0 59 185 29 601.0 0 29 186 34 601.0 0 34 187 26 601.0 0 26 188 54 601.0 0 54 189 34 601.0 0 34 190 26 601.0 0 26 191 13 601.0 0 13 192 16 601.0 0 16 193 20 601.0 0 20 194 33 601.0 0 33 195 39 601.0 0 39 196 45 601.0 0 45 197 182 601.0 0 182 198 140 601.0 0 140 199 278 601.0 0 278 200 278 601.0 0 278 201 219 601.0 0 219 202 998 601.0 0 998 203 413 601.0 0 413 204 273 601.0 0 273 205 67 601.0 0 67 206 55 601.0 0 55 207 33 601.0 0 33 208 72 601.0 0 72 209 29 601.0 0 29 210 28 601.0 0 28 211 31 601.0 0 31 212 25 601.0 0 25 213 33 601.0 0 33 214 33 601.0 0 33 215 36 601.0 0 36 216 33 601.0 0 33 217 149 601.0 0 149 218 161 601.0 0 161 219 213 601.0 0 213 220 54 601.0 0 54 221 78 601.0 0 78 222 1198 601.0 0 1198 223 483 601.0 0 483 224 614 601.0 0 614 225 61 601.0 0 61 226 38 601.0 0 38 227 67 601.0 0 67 228 68 601.0 0 68 229 58 601.0 0 58 230 27 601.0 0 27 231 19 601.0 0 19 232 30 601.0 0 30 233 40 601.0 0 40 234 26 601.0 0 26 235 19 601.0 0 19 236 22 601.0 0 22 237 40 601.0 0 40 238 23 601.0 0 23 239 55 601.0 0 55 240 57 601.0 0 57 241 137 601.0 0 137 242 174 601.0 0 174 243 86 601.0 0 86 244 30 601.0 0 30 245 19 601.0 0 19 246 18 601.0 0 18 247 20 601.0 0 20 248 39 601.0 0 39 249 25 601.0 0 25 250 29 601.0 0 29 251 43 601.0 0 43 252 29 601.0 0 29 253 51 601.0 0 51 254 31 601.0 0 31 255 108 601.0 0 108 256 258 601.0 0 258 257 193 601.0 0 193 258 184 601.0 0 184 259 133 601.0 0 133 260 74 601.0 0 74 261 42 601.0 0 42 262 99 601.0 0 99 263 82 601.0 0 82 264 76 601.0 0 76 265 35 601.0 0 35 266 23 601.0 0 23 267 28 601.0 0 28 268 36 601.0 0 36 269 39 601.0 0 39 270 34 601.0 0 34 271 50 601.0 0 50 272 49 601.0 0 49 273 32 601.0 0 32 274 31 601.0 0 31 275 40 601.0 0 40 276 83 601.0 0 83 277 68 601.0 0 68 278 142 601.0 0 142 279 53 601.0 0 53 280 52 601.0 0 52 281 39 601.0 0 39 282 54 601.0 0 54 283 46 601.0 0 46 284 65 601.0 0 65 285 85 601.0 0 85 286 33 601.0 0 33 287 82 601.0 0 82 288 49 601.0 0 49 289 95 601.0 0 95 290 90 601.0 0 90 291 89 601.0 0 89 292 132 601.0 0 132 293 161 601.0 0 161 294 123 601.0 0 123 295 566 601.0 0 566 296 111 601.0 0 111 297 113 601.0 0 113 298 102 601.0 0 102 299 77 601.0 0 77 300 70 601.0 0 70 301 54 601.0 0 54 Finished in 1.88 s (47 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 39,600 Reads with adapters: 14,956 (37.8%) Reads that were too short: 90 (0.2%) Reads written (passing filters): 14,866 (37.5%) Total basepairs processed: 10,203,813 bp Total written (filtered): 4,091,092 bp (40.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 14956 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 19 0.0 3 0 0 11 8 16 135 0.0 3 0 114 13 8 17 14474 0.0 3 13177 1015 231 51 18 47 0.0 3 3 34 2 8 19 101 0.0 3 14 2 46 39 20 9 0.0 3 1 0 0 8 21 1 0.0 3 0 0 0 1 25 1 0.0 3 1 32 1 0.0 3 0 0 0 1 34 2 0.0 3 0 0 0 2 35 2 0.0 3 0 0 0 2 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 11 0.0 3 0 0 0 11 45 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 3 0.0 3 0 0 0 3 53 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 1 58 1 0.0 3 0 0 1 60 1 0.0 3 0 0 0 1 63 3 0.0 3 0 0 0 3 64 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 3 0.0 3 0 0 0 3 75 3 0.0 3 0 0 1 2 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 82 13 0.0 3 0 0 0 13 83 2 0.0 3 0 0 0 2 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 86 2 0.0 3 0 0 0 2 87 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 90 4 0.0 3 0 0 0 4 91 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 94 3 0.0 3 0 0 0 3 95 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 101 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 111 4 0.0 3 0 0 0 4 112 3 0.0 3 0 0 0 3 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 119 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 126 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 133 3 0.0 3 0 0 0 3 135 1 0.0 3 0 0 0 1 141 3 0.0 3 0 0 0 3 145 5 0.0 3 0 0 0 5 146 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 2 0.0 3 0 0 0 2 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 187 4 0.0 3 0 0 1 3 188 3 0.0 3 0 0 0 3 189 3 0.0 3 0 0 1 2 192 1 0.0 3 0 0 0 1 195 2 0.0 3 0 0 0 2 196 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 208 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 1 275 1 0.0 3 0 0 0 1 279 4 0.0 3 0 0 0 4 280 1 0.0 3 0 0 1 281 4 0.0 3 0 0 0 4 282 2 0.0 3 0 0 0 2 284 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.52 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 14,866 Reads with adapters: 35 (0.2%) Reads written (passing filters): 14,866 (100.0%) Total basepairs processed: 4,091,092 bp Total written (filtered): 4,090,976 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 35 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 62.9% C: 14.3% G: 11.4% T: 11.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 31 232.3 0 31 4 1 58.1 0 1 5 2 14.5 0 2 9 1 0.1 0 0 1