This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTGATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 63.74 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 37,870 (1.5%) Reads that were too short: 2,860 (0.1%) Reads written (passing filters): 35,010 (1.4%) Total basepairs processed: 740,966,583 bp Total written (filtered): 9,579,731 bp (1.3%) === Adapter 1 === Sequence: GTGATC; Type: regular 5'; Length: 6; Trimmed: 37870 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 206 601.0 0 206 7 264 601.0 0 264 8 11704 601.0 0 11704 9 16479 601.0 0 16479 10 23 601.0 0 23 11 2 601.0 0 2 12 1 601.0 0 1 13 1 601.0 0 1 15 1 601.0 0 1 16 1 601.0 0 1 18 1 601.0 0 1 19 1 601.0 0 1 20 1 601.0 0 1 21 1 601.0 0 1 22 2 601.0 0 2 23 3 601.0 0 3 24 2 601.0 0 2 25 1 601.0 0 1 26 3 601.0 0 3 27 3 601.0 0 3 28 1 601.0 0 1 29 4 601.0 0 4 30 3 601.0 0 3 31 7 601.0 0 7 32 20 601.0 0 20 33 15 601.0 0 15 34 17 601.0 0 17 35 82 601.0 0 82 37 57 601.0 0 57 38 8 601.0 0 8 39 24 601.0 0 24 40 16 601.0 0 16 41 3 601.0 0 3 42 4 601.0 0 4 43 5 601.0 0 5 44 5 601.0 0 5 45 3 601.0 0 3 46 6 601.0 0 6 47 2 601.0 0 2 48 5 601.0 0 5 49 2 601.0 0 2 50 3 601.0 0 3 51 1 601.0 0 1 52 4 601.0 0 4 53 4 601.0 0 4 54 6 601.0 0 6 55 6 601.0 0 6 56 4 601.0 0 4 57 3 601.0 0 3 58 31 601.0 0 31 59 67 601.0 0 67 60 127 601.0 0 127 61 2 601.0 0 2 62 5 601.0 0 5 63 14 601.0 0 14 64 4 601.0 0 4 65 6 601.0 0 6 66 8 601.0 0 8 67 11 601.0 0 11 68 14 601.0 0 14 69 11 601.0 0 11 70 13 601.0 0 13 71 13 601.0 0 13 72 8 601.0 0 8 73 11 601.0 0 11 74 12 601.0 0 12 75 13 601.0 0 13 76 170 601.0 0 170 77 193 601.0 0 193 78 457 601.0 0 457 79 12 601.0 0 12 80 103 601.0 0 103 81 57 601.0 0 57 82 146 601.0 0 146 83 22 601.0 0 22 84 1 601.0 0 1 85 6 601.0 0 6 86 6 601.0 0 6 87 10 601.0 0 10 88 2 601.0 0 2 89 8 601.0 0 8 90 11 601.0 0 11 91 3 601.0 0 3 92 3 601.0 0 3 93 1 601.0 0 1 94 11 601.0 0 11 95 9 601.0 0 9 96 49 601.0 0 49 97 10 601.0 0 10 98 4 601.0 0 4 99 14 601.0 0 14 100 111 601.0 0 111 101 479 601.0 0 479 102 419 601.0 0 419 103 6 601.0 0 6 104 22 601.0 0 22 105 13 601.0 0 13 106 7 601.0 0 7 107 8 601.0 0 8 108 20 601.0 0 20 109 35 601.0 0 35 110 357 601.0 0 357 111 273 601.0 0 273 112 332 601.0 0 332 113 5 601.0 0 5 114 11 601.0 0 11 115 17 601.0 0 17 116 6 601.0 0 6 117 37 601.0 0 37 118 5 601.0 0 5 119 3 601.0 0 3 120 8 601.0 0 8 121 4 601.0 0 4 122 7 601.0 0 7 123 4 601.0 0 4 124 3 601.0 0 3 125 5 601.0 0 5 126 5 601.0 0 5 127 94 601.0 0 94 128 21 601.0 0 21 129 10 601.0 0 10 130 85 601.0 0 85 131 3 601.0 0 3 132 5 601.0 0 5 133 3 601.0 0 3 134 6 601.0 0 6 135 5 601.0 0 5 136 57 601.0 0 57 137 9 601.0 0 9 138 15 601.0 0 15 139 3 601.0 0 3 140 4 601.0 0 4 141 10 601.0 0 10 142 12 601.0 0 12 143 10 601.0 0 10 144 4 601.0 0 4 145 14 601.0 0 14 146 17 601.0 0 17 147 106 601.0 0 106 148 19 601.0 0 19 149 79 601.0 0 79 150 25 601.0 0 25 151 95 601.0 0 95 152 421 601.0 0 421 153 17 601.0 0 17 154 2 601.0 0 2 155 11 601.0 0 11 156 5 601.0 0 5 157 5 601.0 0 5 158 6 601.0 0 6 159 4 601.0 0 4 160 19 601.0 0 19 161 7 601.0 0 7 162 39 601.0 0 39 163 5 601.0 0 5 164 7 601.0 0 7 165 8 601.0 0 8 166 12 601.0 0 12 167 8 601.0 0 8 168 66 601.0 0 66 169 67 601.0 0 67 170 260 601.0 0 260 171 10 601.0 0 10 172 3 601.0 0 3 173 8 601.0 0 8 175 5 601.0 0 5 176 13 601.0 0 13 177 13 601.0 0 13 178 5 601.0 0 5 179 4 601.0 0 4 180 5 601.0 0 5 181 4 601.0 0 4 182 32 601.0 0 32 183 41 601.0 0 41 184 16 601.0 0 16 185 9 601.0 0 9 186 6 601.0 0 6 187 5 601.0 0 5 188 6 601.0 0 6 189 18 601.0 0 18 190 5 601.0 0 5 191 4 601.0 0 4 192 7 601.0 0 7 193 7 601.0 0 7 194 9 601.0 0 9 195 12 601.0 0 12 196 6 601.0 0 6 197 24 601.0 0 24 198 27 601.0 0 27 199 60 601.0 0 60 200 30 601.0 0 30 201 36 601.0 0 36 202 118 601.0 0 118 203 55 601.0 0 55 204 39 601.0 0 39 205 9 601.0 0 9 206 11 601.0 0 11 207 14 601.0 0 14 208 21 601.0 0 21 209 10 601.0 0 10 210 12 601.0 0 12 211 7 601.0 0 7 212 10 601.0 0 10 213 6 601.0 0 6 214 6 601.0 0 6 215 9 601.0 0 9 216 11 601.0 0 11 217 32 601.0 0 32 218 41 601.0 0 41 219 46 601.0 0 46 220 15 601.0 0 15 221 5 601.0 0 5 222 199 601.0 0 199 223 100 601.0 0 100 224 161 601.0 0 161 225 28 601.0 0 28 226 11 601.0 0 11 227 17 601.0 0 17 228 15 601.0 0 15 229 13 601.0 0 13 230 6 601.0 0 6 231 6 601.0 0 6 232 13 601.0 0 13 233 8 601.0 0 8 234 8 601.0 0 8 235 8 601.0 0 8 236 5 601.0 0 5 237 18 601.0 0 18 238 19 601.0 0 19 239 18 601.0 0 18 240 23 601.0 0 23 241 43 601.0 0 43 242 44 601.0 0 44 243 17 601.0 0 17 244 12 601.0 0 12 245 6 601.0 0 6 246 2 601.0 0 2 247 5 601.0 0 5 248 3 601.0 0 3 249 10 601.0 0 10 250 10 601.0 0 10 251 13 601.0 0 13 252 12 601.0 0 12 253 41 601.0 0 41 254 10 601.0 0 10 255 28 601.0 0 28 256 54 601.0 0 54 257 58 601.0 0 58 258 50 601.0 0 50 259 46 601.0 0 46 260 18 601.0 0 18 261 10 601.0 0 10 262 23 601.0 0 23 263 36 601.0 0 36 264 17 601.0 0 17 265 9 601.0 0 9 266 7 601.0 0 7 267 10 601.0 0 10 268 16 601.0 0 16 269 11 601.0 0 11 270 12 601.0 0 12 271 17 601.0 0 17 272 6 601.0 0 6 273 13 601.0 0 13 274 11 601.0 0 11 275 9 601.0 0 9 276 24 601.0 0 24 277 12 601.0 0 12 278 31 601.0 0 31 279 8 601.0 0 8 280 11 601.0 0 11 281 12 601.0 0 12 282 13 601.0 0 13 283 12 601.0 0 12 284 19 601.0 0 19 285 13 601.0 0 13 286 12 601.0 0 12 287 20 601.0 0 20 288 15 601.0 0 15 289 18 601.0 0 18 290 29 601.0 0 29 291 38 601.0 0 38 292 68 601.0 0 68 293 81 601.0 0 81 294 51 601.0 0 51 295 317 601.0 0 317 296 53 601.0 0 53 297 35 601.0 0 35 298 34 601.0 0 34 299 38 601.0 0 38 300 23 601.0 0 23 301 31 601.0 0 31 Finished in 1.81 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 35,010 Reads with adapters: 17,021 (48.6%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 17,010 (48.6%) Total basepairs processed: 9,579,731 bp Total written (filtered): 4,626,599 bp (48.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 17021 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 19 62 0.0 3 0 62 20 16878 0.0 4 16622 244 10 2 21 40 0.0 4 6 32 2 22 6 0.0 4 1 0 5 23 17 0.0 4 5 0 0 11 1 24 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 55 1 0.0 4 1 79 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 1 129 1 0.0 4 0 0 0 0 1 143 1 0.0 4 1 183 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 259 4 0.0 4 0 0 0 0 4 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.60 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 17,010 Reads with adapters: 1,761 (10.4%) Reads written (passing filters): 17,010 (100.0%) Total basepairs processed: 4,626,599 bp Total written (filtered): 4,608,370 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1761 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.6% C: 2.9% G: 95.3% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 5 265.8 0 5 4 5 66.4 0 5 5 1 16.6 0 1 6 3 4.2 0 3 7 1 1.0 0 1 8 2 0.3 0 2 9 315 0.1 0 8 307 10 1257 0.0 1 48 1209 11 166 0.0 1 2 164 12 1 0.0 1 0 1 13 1 0.0 1 0 1 223 4 0.0 1 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.