This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATTCTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.46 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 236,130 (9.6%) Reads that were too short: 4,557 (0.2%) Reads written (passing filters): 231,573 (9.4%) Total basepairs processed: 740,966,583 bp Total written (filtered): 45,900,105 bp (6.2%) === Adapter 1 === Sequence: ATTCTA; Type: regular 5'; Length: 6; Trimmed: 236130 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 780 601.0 0 780 7 36703 601.0 0 36703 8 32124 601.0 0 32124 9 25 601.0 0 25 10 13 601.0 0 13 11 78 601.0 0 78 12 102 601.0 0 102 13 72 601.0 0 72 14 6 601.0 0 6 15 6 601.0 0 6 16 3 601.0 0 3 17 12 601.0 0 12 18 2 601.0 0 2 19 7 601.0 0 7 20 13 601.0 0 13 21 11 601.0 0 11 22 12 601.0 0 12 23 7 601.0 0 7 24 11 601.0 0 11 25 19 601.0 0 19 26 37 601.0 0 37 27 54 601.0 0 54 28 15 601.0 0 15 29 3 601.0 0 3 30 6 601.0 0 6 31 15 601.0 0 15 32 19 601.0 0 19 33 19 601.0 0 19 34 17 601.0 0 17 35 15 601.0 0 15 36 14 601.0 0 14 37 12 601.0 0 12 38 70 601.0 0 70 39 34 601.0 0 34 40 16 601.0 0 16 41 6 601.0 0 6 42 16 601.0 0 16 43 19 601.0 0 19 44 22 601.0 0 22 45 14 601.0 0 14 46 6 601.0 0 6 47 10 601.0 0 10 48 10 601.0 0 10 49 6 601.0 0 6 50 11 601.0 0 11 51 16 601.0 0 16 52 14 601.0 0 14 53 7 601.0 0 7 54 16 601.0 0 16 55 14 601.0 0 14 56 13 601.0 0 13 57 7 601.0 0 7 58 7 601.0 0 7 59 16 601.0 0 16 60 27 601.0 0 27 61 8 601.0 0 8 62 7 601.0 0 7 63 6 601.0 0 6 64 11 601.0 0 11 65 9 601.0 0 9 66 5 601.0 0 5 67 10 601.0 0 10 68 11 601.0 0 11 69 23 601.0 0 23 70 11 601.0 0 11 71 17 601.0 0 17 72 14 601.0 0 14 73 19 601.0 0 19 74 97 601.0 0 97 75 16 601.0 0 16 76 18 601.0 0 18 77 4 601.0 0 4 78 7 601.0 0 7 79 19 601.0 0 19 80 75 601.0 0 75 81 17 601.0 0 17 82 64 601.0 0 64 83 8 601.0 0 8 84 9 601.0 0 9 85 19 601.0 0 19 86 12 601.0 0 12 87 8 601.0 0 8 88 10 601.0 0 10 89 19 601.0 0 19 90 13 601.0 0 13 91 20 601.0 0 20 92 23 601.0 0 23 93 10 601.0 0 10 94 21 601.0 0 21 95 7 601.0 0 7 96 26 601.0 0 26 97 37 601.0 0 37 98 70 601.0 0 70 99 29 601.0 0 29 100 19 601.0 0 19 101 15 601.0 0 15 102 68 601.0 0 68 103 72 601.0 0 72 104 114 601.0 0 114 105 110 601.0 0 110 106 59 601.0 0 59 107 75 601.0 0 75 108 65 601.0 0 65 109 36 601.0 0 36 110 27 601.0 0 27 111 36 601.0 0 36 112 81 601.0 0 81 113 51 601.0 0 51 114 51 601.0 0 51 115 288 601.0 0 288 116 25 601.0 0 25 117 20 601.0 0 20 118 14 601.0 0 14 119 26 601.0 0 26 120 38 601.0 0 38 121 44 601.0 0 44 122 158 601.0 0 158 123 205 601.0 0 205 124 437 601.0 0 437 125 408 601.0 0 408 126 105 601.0 0 105 127 154 601.0 0 154 128 93 601.0 0 93 129 100 601.0 0 100 130 287 601.0 0 287 131 110 601.0 0 110 132 153 601.0 0 153 133 132 601.0 0 132 134 145 601.0 0 145 135 363 601.0 0 363 136 301 601.0 0 301 137 502 601.0 0 502 138 883 601.0 0 883 139 1091 601.0 0 1091 140 1767 601.0 0 1767 141 3210 601.0 0 3210 142 33741 601.0 0 33741 143 19921 601.0 0 19921 144 77353 601.0 0 77353 145 321 601.0 0 321 146 42 601.0 0 42 147 64 601.0 0 64 148 24 601.0 0 24 149 62 601.0 0 62 150 28 601.0 0 28 151 36 601.0 0 36 152 36 601.0 0 36 153 34 601.0 0 34 154 31 601.0 0 31 155 50 601.0 0 50 156 71 601.0 0 71 157 93 601.0 0 93 158 93 601.0 0 93 159 125 601.0 0 125 160 241 601.0 0 241 161 511 601.0 0 511 162 3954 601.0 0 3954 163 2203 601.0 0 2203 164 8177 601.0 0 8177 165 32 601.0 0 32 166 17 601.0 0 17 167 27 601.0 0 27 168 38 601.0 0 38 169 59 601.0 0 59 170 11 601.0 0 11 171 8 601.0 0 8 172 15 601.0 0 15 173 11 601.0 0 11 174 4 601.0 0 4 175 16 601.0 0 16 176 19 601.0 0 19 177 57 601.0 0 57 178 44 601.0 0 44 179 25 601.0 0 25 180 14 601.0 0 14 181 15 601.0 0 15 182 14 601.0 0 14 183 21 601.0 0 21 184 18 601.0 0 18 185 29 601.0 0 29 186 9 601.0 0 9 187 29 601.0 0 29 188 47 601.0 0 47 189 85 601.0 0 85 190 190 601.0 0 190 191 10 601.0 0 10 192 9 601.0 0 9 193 18 601.0 0 18 194 5 601.0 0 5 195 15 601.0 0 15 196 21 601.0 0 21 197 13 601.0 0 13 198 12 601.0 0 12 199 20 601.0 0 20 200 11 601.0 0 11 201 18 601.0 0 18 202 14 601.0 0 14 203 15 601.0 0 15 204 10 601.0 0 10 205 12 601.0 0 12 206 16 601.0 0 16 207 36 601.0 0 36 208 22 601.0 0 22 209 30 601.0 0 30 210 71 601.0 0 71 211 108 601.0 0 108 212 148 601.0 0 148 213 38 601.0 0 38 214 77 601.0 0 77 215 107 601.0 0 107 216 280 601.0 0 280 217 127 601.0 0 127 218 26 601.0 0 26 219 14 601.0 0 14 220 12 601.0 0 12 221 31 601.0 0 31 222 29 601.0 0 29 223 50 601.0 0 50 224 46 601.0 0 46 225 48 601.0 0 48 226 53 601.0 0 53 227 69 601.0 0 69 228 20 601.0 0 20 229 19 601.0 0 19 230 23 601.0 0 23 231 16 601.0 0 16 232 91 601.0 0 91 233 65 601.0 0 65 234 48 601.0 0 48 235 11 601.0 0 11 236 19 601.0 0 19 237 41 601.0 0 41 238 45 601.0 0 45 239 37 601.0 0 37 240 42 601.0 0 42 241 32 601.0 0 32 242 96 601.0 0 96 243 23 601.0 0 23 244 18 601.0 0 18 245 28 601.0 0 28 246 19 601.0 0 19 247 26 601.0 0 26 248 14 601.0 0 14 249 13 601.0 0 13 250 24 601.0 0 24 251 16 601.0 0 16 252 21 601.0 0 21 253 16 601.0 0 16 254 14 601.0 0 14 255 42 601.0 0 42 256 53 601.0 0 53 257 93 601.0 0 93 258 53 601.0 0 53 259 52 601.0 0 52 260 51 601.0 0 51 261 83 601.0 0 83 262 131 601.0 0 131 263 85 601.0 0 85 264 56 601.0 0 56 265 27 601.0 0 27 266 23 601.0 0 23 267 18 601.0 0 18 268 28 601.0 0 28 269 43 601.0 0 43 270 40 601.0 0 40 271 32 601.0 0 32 272 19 601.0 0 19 273 13 601.0 0 13 274 42 601.0 0 42 275 29 601.0 0 29 276 60 601.0 0 60 277 37 601.0 0 37 278 77 601.0 0 77 279 180 601.0 0 180 280 93 601.0 0 93 281 73 601.0 0 73 282 46 601.0 0 46 283 61 601.0 0 61 284 48 601.0 0 48 285 46 601.0 0 46 286 31 601.0 0 31 287 38 601.0 0 38 288 29 601.0 0 29 289 28 601.0 0 28 290 27 601.0 0 27 291 41 601.0 0 41 292 19 601.0 0 19 293 23 601.0 0 23 294 29 601.0 0 29 295 48 601.0 0 48 296 55 601.0 0 55 297 41 601.0 0 41 298 25 601.0 0 25 299 24 601.0 0 24 300 27 601.0 0 27 301 12 601.0 0 12 Finished in 8.60 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 231,573 Reads with adapters: 36,665 (15.8%) Reads that were too short: 122 (0.1%) Reads written (passing filters): 36,543 (15.8%) Total basepairs processed: 45,900,105 bp Total written (filtered): 10,116,663 bp (22.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 36665 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 11 0.0 2 0 0 0 11 15 58 0.0 3 0 0 48 10 16 785 0.0 3 0 656 85 44 17 35301 0.0 3 32077 2588 515 121 18 113 0.0 3 10 80 9 14 19 194 0.0 3 2 0 98 94 20 33 0.0 3 2 1 1 29 22 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 36 1 0.0 3 1 37 1 0.0 3 1 44 1 0.0 3 1 48 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 1 1 59 15 0.0 3 0 0 0 15 62 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 0 1 71 3 0.0 3 0 0 0 3 76 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 90 2 0.0 3 0 0 0 2 100 2 0.0 3 0 0 0 2 101 1 0.0 3 0 1 103 5 0.0 3 0 0 0 5 105 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 4 0.0 3 0 0 0 4 115 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 3 0.0 3 0 0 0 3 144 4 0.0 3 0 0 0 4 145 6 0.0 3 0 0 1 5 146 12 0.0 3 0 0 0 12 147 9 0.0 3 0 0 0 9 150 2 0.0 3 0 0 0 2 153 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 156 9 0.0 3 0 0 0 9 159 2 0.0 3 0 0 0 2 164 7 0.0 3 0 0 0 7 166 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 183 2 0.0 3 0 0 0 2 184 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 4 0.0 3 0 0 0 4 207 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 1 221 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 227 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 250 1 0.0 3 0 0 0 1 260 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 1 0.0 3 0 0 0 1 281 10 0.0 3 0 0 0 10 282 4 0.0 3 0 0 1 3 283 1 0.0 3 0 0 0 1 284 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.29 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 36,543 Reads with adapters: 155 (0.4%) Reads written (passing filters): 36,543 (100.0%) Total basepairs processed: 10,116,663 bp Total written (filtered): 10,116,179 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 155 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 25.8% C: 18.1% G: 23.9% T: 32.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 145 571.0 0 145 4 5 142.7 0 5 5 1 35.7 0 1 6 4 8.9 0 4