This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATTCTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.10 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 285,391 (11.6%) Reads that were too short: 3,021 (0.1%) Reads written (passing filters): 282,370 (11.5%) Total basepairs processed: 740,966,583 bp Total written (filtered): 54,380,685 bp (7.3%) === Adapter 1 === Sequence: ATTCTA; Type: regular 5'; Length: 6; Trimmed: 285391 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 636 601.0 0 636 7 32545 601.0 0 32545 8 40791 601.0 0 40791 9 31 601.0 0 31 10 15 601.0 0 15 11 38 601.0 0 38 12 89 601.0 0 89 13 20 601.0 0 20 14 6 601.0 0 6 15 6 601.0 0 6 16 3 601.0 0 3 17 9 601.0 0 9 18 6 601.0 0 6 19 10 601.0 0 10 20 12 601.0 0 12 21 17 601.0 0 17 22 5 601.0 0 5 23 6 601.0 0 6 24 11 601.0 0 11 25 16 601.0 0 16 26 9 601.0 0 9 27 25 601.0 0 25 28 11 601.0 0 11 29 3 601.0 0 3 30 10 601.0 0 10 31 12 601.0 0 12 32 10 601.0 0 10 33 14 601.0 0 14 34 12 601.0 0 12 35 10 601.0 0 10 36 5 601.0 0 5 37 2 601.0 0 2 38 7 601.0 0 7 39 6 601.0 0 6 40 2 601.0 0 2 41 12 601.0 0 12 42 8 601.0 0 8 43 24 601.0 0 24 44 23 601.0 0 23 45 15 601.0 0 15 46 8 601.0 0 8 47 9 601.0 0 9 48 6 601.0 0 6 49 5 601.0 0 5 50 12 601.0 0 12 51 12 601.0 0 12 52 6 601.0 0 6 53 4 601.0 0 4 54 15 601.0 0 15 55 11 601.0 0 11 56 9 601.0 0 9 57 6 601.0 0 6 58 9 601.0 0 9 59 14 601.0 0 14 60 19 601.0 0 19 61 11 601.0 0 11 62 1 601.0 0 1 63 6 601.0 0 6 64 11 601.0 0 11 65 7 601.0 0 7 66 7 601.0 0 7 67 12 601.0 0 12 68 7 601.0 0 7 69 12 601.0 0 12 70 13 601.0 0 13 71 12 601.0 0 12 72 12 601.0 0 12 73 11 601.0 0 11 74 103 601.0 0 103 75 18 601.0 0 18 76 6 601.0 0 6 77 4 601.0 0 4 78 11 601.0 0 11 79 16 601.0 0 16 80 85 601.0 0 85 81 8 601.0 0 8 82 64 601.0 0 64 83 7 601.0 0 7 84 14 601.0 0 14 85 26 601.0 0 26 86 13 601.0 0 13 87 17 601.0 0 17 88 11 601.0 0 11 89 18 601.0 0 18 90 17 601.0 0 17 91 18 601.0 0 18 92 19 601.0 0 19 93 15 601.0 0 15 94 22 601.0 0 22 95 12 601.0 0 12 96 32 601.0 0 32 97 25 601.0 0 25 98 55 601.0 0 55 99 28 601.0 0 28 100 10 601.0 0 10 101 16 601.0 0 16 102 32 601.0 0 32 103 58 601.0 0 58 104 100 601.0 0 100 105 103 601.0 0 103 106 59 601.0 0 59 107 49 601.0 0 49 108 82 601.0 0 82 109 27 601.0 0 27 110 24 601.0 0 24 111 36 601.0 0 36 112 79 601.0 0 79 113 42 601.0 0 42 114 43 601.0 0 43 115 352 601.0 0 352 116 28 601.0 0 28 117 23 601.0 0 23 118 28 601.0 0 28 119 22 601.0 0 22 120 25 601.0 0 25 121 38 601.0 0 38 122 201 601.0 0 201 123 201 601.0 0 201 124 379 601.0 0 379 125 596 601.0 0 596 126 114 601.0 0 114 127 174 601.0 0 174 128 109 601.0 0 109 129 115 601.0 0 115 130 344 601.0 0 344 131 137 601.0 0 137 132 194 601.0 0 194 133 139 601.0 0 139 134 178 601.0 0 178 135 400 601.0 0 400 136 270 601.0 0 270 137 586 601.0 0 586 138 992 601.0 0 992 139 1134 601.0 0 1134 140 1988 601.0 0 1988 141 3125 601.0 0 3125 142 44942 601.0 0 44942 143 24514 601.0 0 24514 144 103295 601.0 0 103295 145 399 601.0 0 399 146 44 601.0 0 44 147 44 601.0 0 44 148 23 601.0 0 23 149 45 601.0 0 45 150 41 601.0 0 41 151 28 601.0 0 28 152 34 601.0 0 34 153 44 601.0 0 44 154 16 601.0 0 16 155 55 601.0 0 55 156 38 601.0 0 38 157 60 601.0 0 60 158 90 601.0 0 90 159 115 601.0 0 115 160 244 601.0 0 244 161 423 601.0 0 423 162 5404 601.0 0 5404 163 2598 601.0 0 2598 164 11179 601.0 0 11179 165 49 601.0 0 49 166 10 601.0 0 10 167 21 601.0 0 21 168 17 601.0 0 17 169 20 601.0 0 20 170 10 601.0 0 10 171 7 601.0 0 7 172 5 601.0 0 5 173 3 601.0 0 3 174 6 601.0 0 6 175 7 601.0 0 7 176 14 601.0 0 14 177 20 601.0 0 20 178 17 601.0 0 17 179 7 601.0 0 7 180 6 601.0 0 6 181 5 601.0 0 5 182 5 601.0 0 5 183 9 601.0 0 9 184 6 601.0 0 6 185 13 601.0 0 13 186 5 601.0 0 5 187 8 601.0 0 8 188 35 601.0 0 35 189 63 601.0 0 63 190 144 601.0 0 144 191 8 601.0 0 8 192 4 601.0 0 4 193 9 601.0 0 9 194 7 601.0 0 7 195 13 601.0 0 13 196 12 601.0 0 12 197 12 601.0 0 12 198 5 601.0 0 5 199 11 601.0 0 11 200 12 601.0 0 12 201 15 601.0 0 15 202 6 601.0 0 6 203 7 601.0 0 7 204 11 601.0 0 11 205 9 601.0 0 9 206 16 601.0 0 16 207 7 601.0 0 7 208 22 601.0 0 22 209 19 601.0 0 19 210 30 601.0 0 30 211 32 601.0 0 32 212 27 601.0 0 27 213 16 601.0 0 16 214 34 601.0 0 34 215 58 601.0 0 58 216 128 601.0 0 128 217 56 601.0 0 56 218 12 601.0 0 12 219 9 601.0 0 9 220 10 601.0 0 10 221 10 601.0 0 10 222 18 601.0 0 18 223 28 601.0 0 28 224 22 601.0 0 22 225 27 601.0 0 27 226 65 601.0 0 65 227 104 601.0 0 104 228 10 601.0 0 10 229 12 601.0 0 12 230 12 601.0 0 12 231 20 601.0 0 20 232 13 601.0 0 13 233 18 601.0 0 18 234 18 601.0 0 18 235 11 601.0 0 11 236 14 601.0 0 14 237 39 601.0 0 39 238 46 601.0 0 46 239 30 601.0 0 30 240 48 601.0 0 48 241 23 601.0 0 23 242 170 601.0 0 170 243 20 601.0 0 20 244 12 601.0 0 12 245 23 601.0 0 23 246 21 601.0 0 21 247 10 601.0 0 10 248 13 601.0 0 13 249 12 601.0 0 12 250 15 601.0 0 15 251 7 601.0 0 7 252 17 601.0 0 17 253 17 601.0 0 17 254 14 601.0 0 14 255 48 601.0 0 48 256 30 601.0 0 30 257 115 601.0 0 115 258 34 601.0 0 34 259 29 601.0 0 29 260 33 601.0 0 33 261 64 601.0 0 64 262 97 601.0 0 97 263 83 601.0 0 83 264 15 601.0 0 15 265 13 601.0 0 13 266 16 601.0 0 16 267 13 601.0 0 13 268 14 601.0 0 14 269 27 601.0 0 27 270 17 601.0 0 17 271 17 601.0 0 17 272 14 601.0 0 14 273 15 601.0 0 15 274 16 601.0 0 16 275 12 601.0 0 12 276 23 601.0 0 23 277 20 601.0 0 20 278 93 601.0 0 93 279 109 601.0 0 109 280 72 601.0 0 72 281 39 601.0 0 39 282 27 601.0 0 27 283 33 601.0 0 33 284 23 601.0 0 23 285 21 601.0 0 21 286 16 601.0 0 16 287 32 601.0 0 32 288 22 601.0 0 22 289 21 601.0 0 21 290 20 601.0 0 20 291 12 601.0 0 12 292 24 601.0 0 24 293 21 601.0 0 21 294 19 601.0 0 19 295 34 601.0 0 34 296 52 601.0 0 52 297 41 601.0 0 41 298 24 601.0 0 24 299 18 601.0 0 18 300 26 601.0 0 26 301 9 601.0 0 9 Finished in 11.03 s (39 us/read; 1.54 M reads/minute). === Summary === Total reads processed: 282,370 Reads with adapters: 41,955 (14.9%) Reads that were too short: 10 (0.0%) Reads written (passing filters): 41,945 (14.9%) Total basepairs processed: 54,380,685 bp Total written (filtered): 11,426,323 bp (21.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 41955 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 4 19 114 0.0 3 0 109 4 0 1 20 41441 0.0 4 40745 678 16 2 21 107 0.0 4 11 95 1 22 11 0.0 4 1 0 10 23 31 0.0 4 2 1 0 27 1 34 1 0.0 4 1 37 6 0.0 4 0 0 0 0 6 38 1 0.0 4 0 0 0 0 1 55 1 0.0 4 1 84 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 127 224 0.0 4 0 0 0 1 223 140 1 0.0 4 0 0 1 153 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 190 1 0.0 4 1 209 2 0.0 4 0 0 0 0 2 228 2 0.0 4 0 0 0 0 2 257 1 0.0 4 0 0 0 0 1 285 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.48 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 41,945 Reads with adapters: 4,244 (10.1%) Reads written (passing filters): 41,945 (100.0%) Total basepairs processed: 11,426,323 bp Total written (filtered): 11,375,535 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4244 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 5.3% G: 91.6% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 37 655.4 0 37 4 24 163.8 0 24 5 2 41.0 0 2 8 1 0.6 0 1 9 180 0.2 0 8 172 10 102 0.0 1 3 99 11 3870 0.0 1 25 3845 12 3 0.0 1 0 3 14 1 0.0 1 0 1 209 5 0.0 1 5 224 18 0.0 1 16 2 226 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.