This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGTAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.13 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 64,277 (2.6%) Reads that were too short: 12,619 (0.5%) Reads written (passing filters): 51,658 (2.1%) Total basepairs processed: 740,966,583 bp Total written (filtered): 11,677,151 bp (1.6%) === Adapter 1 === Sequence: AGGTAA; Type: regular 5'; Length: 6; Trimmed: 64277 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 370 601.0 0 370 7 13000 601.0 0 13000 8 183 601.0 0 183 9 10089 601.0 0 10089 10 74 601.0 0 74 11 56 601.0 0 56 12 48 601.0 0 48 13 64 601.0 0 64 14 58 601.0 0 58 15 58 601.0 0 58 16 81 601.0 0 81 17 77 601.0 0 77 18 62 601.0 0 62 19 39 601.0 0 39 20 43 601.0 0 43 21 65 601.0 0 65 22 64 601.0 0 64 23 45 601.0 0 45 24 60 601.0 0 60 25 53 601.0 0 53 26 59 601.0 0 59 27 49 601.0 0 49 28 62 601.0 0 62 29 68 601.0 0 68 30 99 601.0 0 99 31 120 601.0 0 120 32 106 601.0 0 106 33 97 601.0 0 97 34 72 601.0 0 72 35 45 601.0 0 45 36 81 601.0 0 81 37 143 601.0 0 143 38 77 601.0 0 77 39 76 601.0 0 76 40 62 601.0 0 62 41 77 601.0 0 77 42 82 601.0 0 82 43 47 601.0 0 47 44 75 601.0 0 75 45 77 601.0 0 77 46 60 601.0 0 60 47 60 601.0 0 60 48 61 601.0 0 61 49 84 601.0 0 84 50 79 601.0 0 79 51 119 601.0 0 119 52 70 601.0 0 70 53 59 601.0 0 59 54 79 601.0 0 79 55 63 601.0 0 63 56 80 601.0 0 80 57 44 601.0 0 44 58 66 601.0 0 66 59 81 601.0 0 81 60 67 601.0 0 67 61 59 601.0 0 59 62 68 601.0 0 68 63 92 601.0 0 92 64 51 601.0 0 51 65 83 601.0 0 83 66 105 601.0 0 105 67 110 601.0 0 110 68 884 601.0 0 884 69 546 601.0 0 546 70 668 601.0 0 668 71 62 601.0 0 62 72 71 601.0 0 71 73 64 601.0 0 64 74 60 601.0 0 60 75 61 601.0 0 61 76 39 601.0 0 39 77 61 601.0 0 61 78 62 601.0 0 62 79 69 601.0 0 69 80 256 601.0 0 256 81 92 601.0 0 92 82 69 601.0 0 69 83 62 601.0 0 62 84 67 601.0 0 67 85 76 601.0 0 76 86 57 601.0 0 57 87 49 601.0 0 49 88 80 601.0 0 80 89 68 601.0 0 68 90 70 601.0 0 70 91 78 601.0 0 78 92 55 601.0 0 55 93 56 601.0 0 56 94 71 601.0 0 71 95 69 601.0 0 69 96 71 601.0 0 71 97 66 601.0 0 66 98 87 601.0 0 87 99 83 601.0 0 83 100 71 601.0 0 71 101 83 601.0 0 83 102 68 601.0 0 68 103 89 601.0 0 89 104 82 601.0 0 82 105 73 601.0 0 73 106 56 601.0 0 56 107 74 601.0 0 74 108 73 601.0 0 73 109 71 601.0 0 71 110 72 601.0 0 72 111 61 601.0 0 61 112 54 601.0 0 54 113 75 601.0 0 75 114 70 601.0 0 70 115 61 601.0 0 61 116 217 601.0 0 217 117 107 601.0 0 107 118 100 601.0 0 100 119 92 601.0 0 92 120 70 601.0 0 70 121 124 601.0 0 124 122 103 601.0 0 103 123 81 601.0 0 81 124 140 601.0 0 140 125 73 601.0 0 73 126 118 601.0 0 118 127 169 601.0 0 169 128 121 601.0 0 121 129 59 601.0 0 59 130 97 601.0 0 97 131 110 601.0 0 110 132 108 601.0 0 108 133 223 601.0 0 223 134 386 601.0 0 386 135 606 601.0 0 606 136 200 601.0 0 200 137 116 601.0 0 116 138 251 601.0 0 251 139 109 601.0 0 109 140 68 601.0 0 68 141 67 601.0 0 67 142 135 601.0 0 135 143 103 601.0 0 103 144 137 601.0 0 137 145 82 601.0 0 82 146 108 601.0 0 108 147 77 601.0 0 77 148 149 601.0 0 149 149 72 601.0 0 72 150 113 601.0 0 113 151 106 601.0 0 106 152 580 601.0 0 580 153 388 601.0 0 388 154 399 601.0 0 399 155 113 601.0 0 113 156 129 601.0 0 129 157 151 601.0 0 151 158 73 601.0 0 73 159 386 601.0 0 386 160 95 601.0 0 95 161 332 601.0 0 332 162 97 601.0 0 97 163 100 601.0 0 100 164 942 601.0 0 942 165 92 601.0 0 92 166 274 601.0 0 274 167 261 601.0 0 261 168 259 601.0 0 259 169 252 601.0 0 252 170 77 601.0 0 77 171 74 601.0 0 74 172 66 601.0 0 66 173 68 601.0 0 68 174 112 601.0 0 112 175 433 601.0 0 433 176 263 601.0 0 263 177 208 601.0 0 208 178 128 601.0 0 128 179 125 601.0 0 125 180 608 601.0 0 608 181 888 601.0 0 888 182 573 601.0 0 573 183 198 601.0 0 198 184 131 601.0 0 131 185 101 601.0 0 101 186 166 601.0 0 166 187 121 601.0 0 121 188 75 601.0 0 75 189 63 601.0 0 63 190 101 601.0 0 101 191 80 601.0 0 80 192 68 601.0 0 68 193 80 601.0 0 80 194 129 601.0 0 129 195 214 601.0 0 214 196 278 601.0 0 278 197 385 601.0 0 385 198 252 601.0 0 252 199 80 601.0 0 80 200 1122 601.0 0 1122 201 1306 601.0 0 1306 202 829 601.0 0 829 203 160 601.0 0 160 204 56 601.0 0 56 205 89 601.0 0 89 206 73 601.0 0 73 207 78 601.0 0 78 208 75 601.0 0 75 209 75 601.0 0 75 210 48 601.0 0 48 211 76 601.0 0 76 212 98 601.0 0 98 213 80 601.0 0 80 214 84 601.0 0 84 215 50 601.0 0 50 216 67 601.0 0 67 217 60 601.0 0 60 218 66 601.0 0 66 219 74 601.0 0 74 220 83 601.0 0 83 221 114 601.0 0 114 222 148 601.0 0 148 223 93 601.0 0 93 224 102 601.0 0 102 225 161 601.0 0 161 226 266 601.0 0 266 227 131 601.0 0 131 228 66 601.0 0 66 229 87 601.0 0 87 230 73 601.0 0 73 231 60 601.0 0 60 232 84 601.0 0 84 233 92 601.0 0 92 234 67 601.0 0 67 235 68 601.0 0 68 236 81 601.0 0 81 237 87 601.0 0 87 238 126 601.0 0 126 239 98 601.0 0 98 240 106 601.0 0 106 241 107 601.0 0 107 242 99 601.0 0 99 243 98 601.0 0 98 244 75 601.0 0 75 245 71 601.0 0 71 246 62 601.0 0 62 247 66 601.0 0 66 248 82 601.0 0 82 249 147 601.0 0 147 250 130 601.0 0 130 251 124 601.0 0 124 252 88 601.0 0 88 253 245 601.0 0 245 254 113 601.0 0 113 255 390 601.0 0 390 256 117 601.0 0 117 257 91 601.0 0 91 258 238 601.0 0 238 259 286 601.0 0 286 260 260 601.0 0 260 261 74 601.0 0 74 262 103 601.0 0 103 263 161 601.0 0 161 264 619 601.0 0 619 265 323 601.0 0 323 266 151 601.0 0 151 267 68 601.0 0 68 268 94 601.0 0 94 269 98 601.0 0 98 270 82 601.0 0 82 271 66 601.0 0 66 272 81 601.0 0 81 273 78 601.0 0 78 274 89 601.0 0 89 275 107 601.0 0 107 276 604 601.0 0 604 277 330 601.0 0 330 278 170 601.0 0 170 279 50 601.0 0 50 280 58 601.0 0 58 281 68 601.0 0 68 282 67 601.0 0 67 283 61 601.0 0 61 284 70 601.0 0 70 285 86 601.0 0 86 286 92 601.0 0 92 287 62 601.0 0 62 288 80 601.0 0 80 289 72 601.0 0 72 290 81 601.0 0 81 291 71 601.0 0 71 292 97 601.0 0 97 293 82 601.0 0 82 294 134 601.0 0 134 295 72 601.0 0 72 296 103 601.0 0 103 297 89 601.0 0 89 298 177 601.0 0 177 299 146 601.0 0 146 300 130 601.0 0 130 301 153 601.0 0 153 Finished in 2.40 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 51,658 Reads with adapters: 12,923 (25.0%) Reads that were too short: 24 (0.0%) Reads written (passing filters): 12,899 (25.0%) Total basepairs processed: 11,677,151 bp Total written (filtered): 3,533,773 bp (30.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 12923 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 19 29 0.0 3 0 26 3 20 12820 0.0 4 12609 204 5 2 21 37 0.0 4 8 29 22 1 0.0 4 0 0 1 23 5 0.0 4 1 0 0 3 1 24 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 1 40 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 58 1 0.0 4 0 0 0 1 63 1 0.0 4 0 0 0 0 1 69 2 0.0 4 1 0 1 71 1 0.0 4 1 86 1 0.0 4 1 87 1 0.0 4 1 88 1 0.0 4 0 0 1 91 2 0.0 4 0 0 0 0 2 92 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 130 4 0.0 4 0 0 0 0 4 144 1 0.0 4 0 0 0 0 1 170 2 0.0 4 0 0 0 0 2 172 1 0.0 4 0 0 0 0 1 228 2 0.0 4 0 0 0 1 1 229 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.44 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 12,899 Reads with adapters: 275 (2.1%) Reads written (passing filters): 12,899 (100.0%) Total basepairs processed: 3,533,773 bp Total written (filtered): 3,528,971 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 275 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.7% C: 5.8% G: 80.7% T: 4.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 26 201.5 0 26 4 2 50.4 0 2 5 1 12.6 0 1 10 1 0.0 1 0 1 11 181 0.0 1 2 179 12 47 0.0 1 5 42 15 7 0.0 1 3 4 16 1 0.0 1 1 225 9 0.0 1 9 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.