This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.93 s (27 us/read; 2.21 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 366,893 (14.9%) Reads that were too short: 22,075 (0.9%) Reads written (passing filters): 344,818 (14.0%) Total basepairs processed: 740,966,583 bp Total written (filtered): 97,480,369 bp (13.2%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 366893 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1844 601.0 0 1844 7 108458 601.0 0 108458 8 3514 601.0 0 3514 9 80524 601.0 0 80524 10 1029 601.0 0 1029 11 1261 601.0 0 1261 12 26819 601.0 0 26819 13 82684 601.0 0 82684 14 13861 601.0 0 13861 15 59 601.0 0 59 16 54 601.0 0 54 17 52 601.0 0 52 18 52 601.0 0 52 19 41 601.0 0 41 20 47 601.0 0 47 21 42 601.0 0 42 22 57 601.0 0 57 23 53 601.0 0 53 24 52 601.0 0 52 25 45 601.0 0 45 26 69 601.0 0 69 27 146 601.0 0 146 28 124 601.0 0 124 29 65 601.0 0 65 30 50 601.0 0 50 31 44 601.0 0 44 32 53 601.0 0 53 33 48 601.0 0 48 34 71 601.0 0 71 35 42 601.0 0 42 36 57 601.0 0 57 37 54 601.0 0 54 38 44 601.0 0 44 39 52 601.0 0 52 40 90 601.0 0 90 41 44 601.0 0 44 42 51 601.0 0 51 43 67 601.0 0 67 44 187 601.0 0 187 45 290 601.0 0 290 46 44 601.0 0 44 47 39 601.0 0 39 48 48 601.0 0 48 49 51 601.0 0 51 50 51 601.0 0 51 51 50 601.0 0 50 52 32 601.0 0 32 53 60 601.0 0 60 54 53 601.0 0 53 55 40 601.0 0 40 56 30 601.0 0 30 57 55 601.0 0 55 58 58 601.0 0 58 59 46 601.0 0 46 60 42 601.0 0 42 61 59 601.0 0 59 62 43 601.0 0 43 63 61 601.0 0 61 64 41 601.0 0 41 65 60 601.0 0 60 66 66 601.0 0 66 67 50 601.0 0 50 68 55 601.0 0 55 69 36 601.0 0 36 70 55 601.0 0 55 71 41 601.0 0 41 72 49 601.0 0 49 73 70 601.0 0 70 74 72 601.0 0 72 75 47 601.0 0 47 76 48 601.0 0 48 77 66 601.0 0 66 78 68 601.0 0 68 79 56 601.0 0 56 80 58 601.0 0 58 81 57 601.0 0 57 82 47 601.0 0 47 83 53 601.0 0 53 84 47 601.0 0 47 85 39 601.0 0 39 86 69 601.0 0 69 87 61 601.0 0 61 88 57 601.0 0 57 89 84 601.0 0 84 90 106 601.0 0 106 91 79 601.0 0 79 92 123 601.0 0 123 93 69 601.0 0 69 94 79 601.0 0 79 95 58 601.0 0 58 96 43 601.0 0 43 97 53 601.0 0 53 98 67 601.0 0 67 99 54 601.0 0 54 100 78 601.0 0 78 101 66 601.0 0 66 102 58 601.0 0 58 103 68 601.0 0 68 104 529 601.0 0 529 105 396 601.0 0 396 106 307 601.0 0 307 107 106 601.0 0 106 108 125 601.0 0 125 109 96 601.0 0 96 110 162 601.0 0 162 111 146 601.0 0 146 112 175 601.0 0 175 113 89 601.0 0 89 114 118 601.0 0 118 115 91 601.0 0 91 116 116 601.0 0 116 117 109 601.0 0 109 118 58 601.0 0 58 119 79 601.0 0 79 120 87 601.0 0 87 121 60 601.0 0 60 122 73 601.0 0 73 123 70 601.0 0 70 124 157 601.0 0 157 125 152 601.0 0 152 126 653 601.0 0 653 127 340 601.0 0 340 128 779 601.0 0 779 129 344 601.0 0 344 130 82 601.0 0 82 131 74 601.0 0 74 132 97 601.0 0 97 133 88 601.0 0 88 134 87 601.0 0 87 135 108 601.0 0 108 136 108 601.0 0 108 137 136 601.0 0 136 138 1014 601.0 0 1014 139 322 601.0 0 322 140 692 601.0 0 692 141 279 601.0 0 279 142 202 601.0 0 202 143 71 601.0 0 71 144 121 601.0 0 121 145 111 601.0 0 111 146 114 601.0 0 114 147 94 601.0 0 94 148 112 601.0 0 112 149 54 601.0 0 54 150 68 601.0 0 68 151 201 601.0 0 201 152 286 601.0 0 286 153 428 601.0 0 428 154 121 601.0 0 121 155 121 601.0 0 121 156 130 601.0 0 130 157 115 601.0 0 115 158 1294 601.0 0 1294 159 1289 601.0 0 1289 160 1361 601.0 0 1361 161 567 601.0 0 567 162 251 601.0 0 251 163 144 601.0 0 144 164 75 601.0 0 75 165 86 601.0 0 86 166 58 601.0 0 58 167 90 601.0 0 90 168 122 601.0 0 122 169 201 601.0 0 201 170 97 601.0 0 97 171 103 601.0 0 103 172 62 601.0 0 62 173 80 601.0 0 80 174 82 601.0 0 82 175 79 601.0 0 79 176 106 601.0 0 106 177 88 601.0 0 88 178 49 601.0 0 49 179 56 601.0 0 56 180 76 601.0 0 76 181 132 601.0 0 132 182 181 601.0 0 181 183 105 601.0 0 105 184 74 601.0 0 74 185 77 601.0 0 77 186 55 601.0 0 55 187 86 601.0 0 86 188 117 601.0 0 117 189 178 601.0 0 178 190 172 601.0 0 172 191 46 601.0 0 46 192 71 601.0 0 71 193 69 601.0 0 69 194 61 601.0 0 61 195 49 601.0 0 49 196 55 601.0 0 55 197 80 601.0 0 80 198 86 601.0 0 86 199 79 601.0 0 79 200 99 601.0 0 99 201 166 601.0 0 166 202 88 601.0 0 88 203 78 601.0 0 78 204 76 601.0 0 76 205 72 601.0 0 72 206 78 601.0 0 78 207 125 601.0 0 125 208 99 601.0 0 99 209 95 601.0 0 95 210 71 601.0 0 71 211 112 601.0 0 112 212 70 601.0 0 70 213 118 601.0 0 118 214 182 601.0 0 182 215 200 601.0 0 200 216 247 601.0 0 247 217 67 601.0 0 67 218 88 601.0 0 88 219 75 601.0 0 75 220 204 601.0 0 204 221 461 601.0 0 461 222 269 601.0 0 269 223 131 601.0 0 131 224 58 601.0 0 58 225 142 601.0 0 142 226 162 601.0 0 162 227 108 601.0 0 108 228 61 601.0 0 61 229 53 601.0 0 53 230 72 601.0 0 72 231 193 601.0 0 193 232 220 601.0 0 220 233 320 601.0 0 320 234 148 601.0 0 148 235 216 601.0 0 216 236 99 601.0 0 99 237 87 601.0 0 87 238 73 601.0 0 73 239 62 601.0 0 62 240 79 601.0 0 79 241 130 601.0 0 130 242 172 601.0 0 172 243 141 601.0 0 141 244 130 601.0 0 130 245 98 601.0 0 98 246 74 601.0 0 74 247 77 601.0 0 77 248 78 601.0 0 78 249 63 601.0 0 63 250 69 601.0 0 69 251 89 601.0 0 89 252 103 601.0 0 103 253 136 601.0 0 136 254 96 601.0 0 96 255 84 601.0 0 84 256 111 601.0 0 111 257 90 601.0 0 90 258 57 601.0 0 57 259 56 601.0 0 56 260 92 601.0 0 92 261 113 601.0 0 113 262 172 601.0 0 172 263 179 601.0 0 179 264 138 601.0 0 138 265 100 601.0 0 100 266 90 601.0 0 90 267 264 601.0 0 264 268 299 601.0 0 299 269 228 601.0 0 228 270 141 601.0 0 141 271 99 601.0 0 99 272 86 601.0 0 86 273 69 601.0 0 69 274 90 601.0 0 90 275 87 601.0 0 87 276 134 601.0 0 134 277 348 601.0 0 348 278 251 601.0 0 251 279 332 601.0 0 332 280 386 601.0 0 386 281 1566 601.0 0 1566 282 2497 601.0 0 2497 283 3357 601.0 0 3357 284 1071 601.0 0 1071 285 84 601.0 0 84 286 129 601.0 0 129 287 112 601.0 0 112 288 597 601.0 0 597 289 385 601.0 0 385 290 378 601.0 0 378 291 221 601.0 0 221 292 200 601.0 0 200 293 92 601.0 0 92 294 121 601.0 0 121 295 128 601.0 0 128 296 109 601.0 0 109 297 72 601.0 0 72 298 61 601.0 0 61 299 46 601.0 0 46 300 49 601.0 0 49 301 89 601.0 0 89 Finished in 15.38 s (45 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 344,818 Reads with adapters: 111,037 (32.2%) Reads that were too short: 801 (0.2%) Reads written (passing filters): 110,236 (32.0%) Total basepairs processed: 97,480,369 bp Total written (filtered): 30,152,610 bp (30.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 111037 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 23 0.0 2 0 0 0 23 15 75 0.0 3 0 0 42 33 16 845 0.0 3 0 645 113 87 17 105884 0.0 3 97117 7036 1383 348 18 242 0.0 3 16 175 22 29 19 445 0.0 3 0 0 255 190 20 185 0.0 3 19 2 0 164 22 2 0.0 3 2 23 1 0.0 3 0 0 0 1 24 1 0.0 3 0 1 26 1 0.0 3 1 28 1 0.0 3 1 31 6 0.0 3 1 0 0 5 32 43 0.0 3 1 0 0 42 34 1 0.0 3 0 0 0 1 35 8 0.0 3 0 0 1 7 36 3 0.0 3 0 1 0 2 39 5 0.0 3 0 0 0 5 40 3 0.0 3 0 0 0 3 41 3 0.0 3 0 0 0 3 42 1 0.0 3 0 0 0 1 43 40 0.0 3 0 0 3 37 45 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 48 3 0.0 3 0 0 0 3 49 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 53 63 0.0 3 0 0 1 62 54 3 0.0 3 0 0 1 2 55 1 0.0 3 0 0 0 1 56 16 0.0 3 0 0 0 16 57 11 0.0 3 0 0 0 11 59 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 67 41 0.0 3 0 0 0 41 68 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 70 12 0.0 3 0 0 0 12 71 11 0.0 3 0 0 0 11 74 1 0.0 3 0 0 0 1 75 2 0.0 3 0 0 1 1 76 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 83 3 0.0 3 0 0 1 2 84 3 0.0 3 0 0 0 3 85 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 3 0.0 3 0 0 0 3 88 17 0.0 3 0 0 0 17 90 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 101 4 0.0 3 0 0 4 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 4 0.0 3 0 0 1 3 105 2 0.0 3 0 0 0 2 107 4 0.0 3 0 0 0 4 108 2 0.0 3 0 0 0 2 109 2 0.0 3 0 0 0 2 110 77 0.0 3 0 0 5 72 111 8 0.0 3 0 0 0 8 112 4 0.0 3 0 0 0 4 113 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 119 2 0.0 3 0 0 1 1 120 5 0.0 3 0 0 0 5 122 1 0.0 3 0 0 0 1 124 10 0.0 3 0 0 0 10 125 2 0.0 3 0 0 1 1 126 4 0.0 3 0 0 0 4 127 8 0.0 3 1 0 0 7 129 4 0.0 3 0 0 0 4 130 1 0.0 3 0 0 0 1 131 3 0.0 3 0 0 0 3 132 4 0.0 3 0 0 0 4 133 6 0.0 3 0 0 0 6 134 2 0.0 3 0 0 0 2 135 2 0.0 3 0 0 0 2 136 2 0.0 3 0 0 0 2 137 5 0.0 3 0 0 0 5 138 4 0.0 3 0 0 0 4 139 2 0.0 3 0 0 0 2 142 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 146 1 0.0 3 0 0 0 1 147 2 0.0 3 0 0 0 2 148 1 0.0 3 0 0 0 1 149 2 0.0 3 0 0 0 2 150 1 0.0 3 0 0 0 1 152 2 0.0 3 0 0 0 2 153 3 0.0 3 0 0 0 3 154 116 0.0 3 0 0 0 116 155 9 0.0 3 0 0 0 9 156 6 0.0 3 0 0 1 5 157 27 0.0 3 0 0 2 25 158 11 0.0 3 0 0 0 11 159 465 0.0 3 0 0 9 456 160 117 0.0 3 0 0 0 117 161 39 0.0 3 0 0 0 39 162 134 0.0 3 0 0 4 130 163 21 0.0 3 0 0 1 20 164 18 0.0 3 0 0 1 17 165 24 0.0 3 0 0 0 24 166 5 0.0 3 0 0 0 5 167 12 0.0 3 0 0 0 12 168 7 0.0 3 0 0 1 6 169 13 0.0 3 0 0 0 13 170 10 0.0 3 0 0 0 10 171 7 0.0 3 0 0 0 7 172 4 0.0 3 0 0 0 4 173 5 0.0 3 0 0 1 4 174 14 0.0 3 0 0 0 14 175 3 0.0 3 0 0 0 3 176 4 0.0 3 0 0 0 4 177 7 0.0 3 0 0 1 6 178 64 0.0 3 0 0 2 62 179 467 0.0 3 0 0 1 466 180 301 0.0 3 0 0 1 300 181 10 0.0 3 0 0 1 9 182 67 0.0 3 0 0 2 65 183 52 0.0 3 0 0 3 49 184 4 0.0 3 0 0 1 3 185 17 0.0 3 0 0 0 17 186 4 0.0 3 0 0 1 3 187 4 0.0 3 0 0 0 4 188 7 0.0 3 0 0 1 6 189 5 0.0 3 0 0 1 4 191 3 0.0 3 0 0 0 3 192 2 0.0 3 0 0 0 2 193 2 0.0 3 0 0 0 2 194 3 0.0 3 0 0 0 3 196 2 0.0 3 0 0 0 2 197 7 0.0 3 0 0 1 6 198 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 201 2 0.0 3 0 0 0 2 203 10 0.0 3 0 0 0 10 204 6 0.0 3 0 0 2 4 205 41 0.0 3 0 0 0 41 206 7 0.0 3 0 0 0 7 208 2 0.0 3 0 0 0 2 209 1 0.0 3 0 0 0 1 210 11 0.0 3 0 0 1 10 211 6 0.0 3 0 0 1 5 212 8 0.0 3 0 0 0 8 213 3 0.0 3 0 0 0 3 214 29 0.0 3 0 0 0 29 215 4 0.0 3 0 0 1 3 216 1 0.0 3 0 0 0 1 217 8 0.0 3 0 0 0 8 218 3 0.0 3 0 0 0 3 221 2 0.0 3 0 0 0 2 222 4 0.0 3 0 0 0 4 223 3 0.0 3 0 0 0 3 224 2 0.0 3 0 0 0 2 226 3 0.0 3 0 0 0 3 227 3 0.0 3 0 0 0 3 229 2 0.0 3 0 0 0 2 230 31 0.0 3 0 0 0 31 231 15 0.0 3 0 0 1 14 232 5 0.0 3 0 0 0 5 233 4 0.0 3 0 0 0 4 234 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 0 2 239 32 0.0 3 0 0 0 32 240 7 0.0 3 0 0 1 6 241 1 0.0 3 0 0 1 242 2 0.0 3 0 0 0 2 243 3 0.0 3 0 0 0 3 245 1 0.0 3 0 0 0 1 246 2 0.0 3 0 0 0 2 247 2 0.0 3 0 0 0 2 249 1 0.0 3 0 0 0 1 250 1 0.0 3 0 0 0 1 251 2 0.0 3 0 0 0 2 252 6 0.0 3 0 0 0 6 253 1 0.0 3 0 0 0 1 254 9 0.0 3 0 0 0 9 255 11 0.0 3 0 0 1 10 256 4 0.0 3 0 0 0 4 257 2 0.0 3 0 0 0 2 258 12 0.0 3 0 0 0 12 259 7 0.0 3 0 0 0 7 260 2 0.0 3 0 0 0 2 261 5 0.0 3 0 0 0 5 262 6 0.0 3 0 0 0 6 264 1 0.0 3 0 0 0 1 265 3 0.0 3 0 0 0 3 266 4 0.0 3 0 0 0 4 267 1 0.0 3 0 0 0 1 268 2 0.0 3 0 0 0 2 269 7 0.0 3 0 0 1 6 270 6 0.0 3 0 0 0 6 271 23 0.0 3 0 0 1 22 272 21 0.0 3 0 0 1 20 273 13 0.0 3 0 0 0 13 274 23 0.0 3 0 0 0 23 275 11 0.0 3 0 0 1 10 276 5 0.0 3 0 0 1 4 277 12 0.0 3 0 0 0 12 278 6 0.0 3 0 0 0 6 279 18 0.0 3 0 0 0 18 280 54 0.0 3 0 0 1 53 281 78 0.0 3 0 0 4 74 282 20 0.0 3 0 0 2 18 283 10 0.0 3 0 0 0 10 284 3 0.0 3 0 0 0 3 285 2 0.0 3 0 0 0 2 286 12 0.0 3 0 0 0 12 287 2 0.0 3 0 0 1 1 288 13 0.0 3 0 0 0 13 289 13 0.0 3 0 0 0 13 290 1 0.0 3 0 0 0 1 291 2 0.0 3 0 0 0 2 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.77 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 110,236 Reads with adapters: 706 (0.6%) Reads written (passing filters): 110,236 (100.0%) Total basepairs processed: 30,152,610 bp Total written (filtered): 30,150,345 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 706 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 18.7% C: 34.6% G: 24.1% T: 22.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 613 1722.4 0 613 4 63 430.6 0 63 5 15 107.7 0 15 6 13 26.9 0 13 9 1 0.4 0 0 1 12 1 0.0 1 0 1