This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCTAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.96 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 350,359 (14.2%) Reads that were too short: 12,544 (0.5%) Reads written (passing filters): 337,815 (13.7%) Total basepairs processed: 740,966,583 bp Total written (filtered): 96,403,083 bp (13.0%) === Adapter 1 === Sequence: ACCTAT; Type: regular 5'; Length: 6; Trimmed: 350359 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1583 601.0 0 1583 7 89660 601.0 0 89660 8 1761 601.0 0 1761 9 113416 601.0 0 113416 10 834 601.0 0 834 11 964 601.0 0 964 12 22872 601.0 0 22872 13 78179 601.0 0 78179 14 11996 601.0 0 11996 15 41 601.0 0 41 16 46 601.0 0 46 17 44 601.0 0 44 18 32 601.0 0 32 19 27 601.0 0 27 20 41 601.0 0 41 21 35 601.0 0 35 22 35 601.0 0 35 23 31 601.0 0 31 24 37 601.0 0 37 25 30 601.0 0 30 26 37 601.0 0 37 27 38 601.0 0 38 28 32 601.0 0 32 29 29 601.0 0 29 30 34 601.0 0 34 31 31 601.0 0 31 32 27 601.0 0 27 33 28 601.0 0 28 34 25 601.0 0 25 35 26 601.0 0 26 36 37 601.0 0 37 37 38 601.0 0 38 38 48 601.0 0 48 39 31 601.0 0 31 40 52 601.0 0 52 41 41 601.0 0 41 42 43 601.0 0 43 43 48 601.0 0 48 44 116 601.0 0 116 45 146 601.0 0 146 46 35 601.0 0 35 47 24 601.0 0 24 48 39 601.0 0 39 49 45 601.0 0 45 50 45 601.0 0 45 51 37 601.0 0 37 52 48 601.0 0 48 53 53 601.0 0 53 54 35 601.0 0 35 55 26 601.0 0 26 56 31 601.0 0 31 57 30 601.0 0 30 58 48 601.0 0 48 59 34 601.0 0 34 60 31 601.0 0 31 61 27 601.0 0 27 62 27 601.0 0 27 63 46 601.0 0 46 64 33 601.0 0 33 65 50 601.0 0 50 66 45 601.0 0 45 67 47 601.0 0 47 68 37 601.0 0 37 69 39 601.0 0 39 70 26 601.0 0 26 71 30 601.0 0 30 72 38 601.0 0 38 73 43 601.0 0 43 74 52 601.0 0 52 75 33 601.0 0 33 76 38 601.0 0 38 77 47 601.0 0 47 78 54 601.0 0 54 79 52 601.0 0 52 80 51 601.0 0 51 81 40 601.0 0 40 82 49 601.0 0 49 83 42 601.0 0 42 84 49 601.0 0 49 85 44 601.0 0 44 86 61 601.0 0 61 87 63 601.0 0 63 88 48 601.0 0 48 89 52 601.0 0 52 90 79 601.0 0 79 91 69 601.0 0 69 92 90 601.0 0 90 93 54 601.0 0 54 94 66 601.0 0 66 95 50 601.0 0 50 96 44 601.0 0 44 97 35 601.0 0 35 98 54 601.0 0 54 99 47 601.0 0 47 100 47 601.0 0 47 101 37 601.0 0 37 102 61 601.0 0 61 103 53 601.0 0 53 104 202 601.0 0 202 105 140 601.0 0 140 106 139 601.0 0 139 107 60 601.0 0 60 108 69 601.0 0 69 109 51 601.0 0 51 110 76 601.0 0 76 111 80 601.0 0 80 112 75 601.0 0 75 113 81 601.0 0 81 114 46 601.0 0 46 115 56 601.0 0 56 116 107 601.0 0 107 117 81 601.0 0 81 118 54 601.0 0 54 119 64 601.0 0 64 120 64 601.0 0 64 121 62 601.0 0 62 122 57 601.0 0 57 123 58 601.0 0 58 124 76 601.0 0 76 125 95 601.0 0 95 126 473 601.0 0 473 127 288 601.0 0 288 128 712 601.0 0 712 129 214 601.0 0 214 130 55 601.0 0 55 131 47 601.0 0 47 132 65 601.0 0 65 133 58 601.0 0 58 134 61 601.0 0 61 135 63 601.0 0 63 136 68 601.0 0 68 137 75 601.0 0 75 138 185 601.0 0 185 139 116 601.0 0 116 140 255 601.0 0 255 141 153 601.0 0 153 142 133 601.0 0 133 143 71 601.0 0 71 144 68 601.0 0 68 145 69 601.0 0 69 146 57 601.0 0 57 147 63 601.0 0 63 148 48 601.0 0 48 149 46 601.0 0 46 150 55 601.0 0 55 151 146 601.0 0 146 152 186 601.0 0 186 153 230 601.0 0 230 154 69 601.0 0 69 155 76 601.0 0 76 156 101 601.0 0 101 157 100 601.0 0 100 158 1376 601.0 0 1376 159 1027 601.0 0 1027 160 1247 601.0 0 1247 161 195 601.0 0 195 162 142 601.0 0 142 163 74 601.0 0 74 164 58 601.0 0 58 165 57 601.0 0 57 166 38 601.0 0 38 167 46 601.0 0 46 168 85 601.0 0 85 169 295 601.0 0 295 170 95 601.0 0 95 171 84 601.0 0 84 172 44 601.0 0 44 173 44 601.0 0 44 174 66 601.0 0 66 175 54 601.0 0 54 176 54 601.0 0 54 177 43 601.0 0 43 178 45 601.0 0 45 179 64 601.0 0 64 180 46 601.0 0 46 181 59 601.0 0 59 182 66 601.0 0 66 183 58 601.0 0 58 184 47 601.0 0 47 185 44 601.0 0 44 186 50 601.0 0 50 187 37 601.0 0 37 188 56 601.0 0 56 189 73 601.0 0 73 190 112 601.0 0 112 191 33 601.0 0 33 192 64 601.0 0 64 193 54 601.0 0 54 194 47 601.0 0 47 195 43 601.0 0 43 196 39 601.0 0 39 197 51 601.0 0 51 198 56 601.0 0 56 199 53 601.0 0 53 200 57 601.0 0 57 201 57 601.0 0 57 202 59 601.0 0 59 203 49 601.0 0 49 204 61 601.0 0 61 205 38 601.0 0 38 206 44 601.0 0 44 207 54 601.0 0 54 208 58 601.0 0 58 209 63 601.0 0 63 210 60 601.0 0 60 211 49 601.0 0 49 212 43 601.0 0 43 213 44 601.0 0 44 214 112 601.0 0 112 215 99 601.0 0 99 216 146 601.0 0 146 217 38 601.0 0 38 218 84 601.0 0 84 219 52 601.0 0 52 220 95 601.0 0 95 221 197 601.0 0 197 222 183 601.0 0 183 223 74 601.0 0 74 224 66 601.0 0 66 225 143 601.0 0 143 226 140 601.0 0 140 227 82 601.0 0 82 228 36 601.0 0 36 229 52 601.0 0 52 230 47 601.0 0 47 231 76 601.0 0 76 232 100 601.0 0 100 233 265 601.0 0 265 234 138 601.0 0 138 235 250 601.0 0 250 236 81 601.0 0 81 237 76 601.0 0 76 238 56 601.0 0 56 239 38 601.0 0 38 240 49 601.0 0 49 241 80 601.0 0 80 242 81 601.0 0 81 243 67 601.0 0 67 244 67 601.0 0 67 245 49 601.0 0 49 246 54 601.0 0 54 247 50 601.0 0 50 248 39 601.0 0 39 249 61 601.0 0 61 250 58 601.0 0 58 251 69 601.0 0 69 252 69 601.0 0 69 253 73 601.0 0 73 254 58 601.0 0 58 255 47 601.0 0 47 256 67 601.0 0 67 257 75 601.0 0 75 258 61 601.0 0 61 259 49 601.0 0 49 260 71 601.0 0 71 261 82 601.0 0 82 262 167 601.0 0 167 263 194 601.0 0 194 264 152 601.0 0 152 265 52 601.0 0 52 266 50 601.0 0 50 267 149 601.0 0 149 268 133 601.0 0 133 269 105 601.0 0 105 270 77 601.0 0 77 271 66 601.0 0 66 272 54 601.0 0 54 273 58 601.0 0 58 274 55 601.0 0 55 275 81 601.0 0 81 276 79 601.0 0 79 277 321 601.0 0 321 278 259 601.0 0 259 279 260 601.0 0 260 280 182 601.0 0 182 281 618 601.0 0 618 282 1297 601.0 0 1297 283 1146 601.0 0 1146 284 457 601.0 0 457 285 47 601.0 0 47 286 94 601.0 0 94 287 97 601.0 0 97 288 295 601.0 0 295 289 200 601.0 0 200 290 233 601.0 0 233 291 132 601.0 0 132 292 116 601.0 0 116 293 70 601.0 0 70 294 84 601.0 0 84 295 83 601.0 0 83 296 85 601.0 0 85 297 62 601.0 0 62 298 53 601.0 0 53 299 35 601.0 0 35 300 47 601.0 0 47 301 75 601.0 0 75 Finished in 16.12 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 337,815 Reads with adapters: 117,468 (34.8%) Reads that were too short: 42 (0.0%) Reads written (passing filters): 117,426 (34.8%) Total basepairs processed: 96,403,083 bp Total written (filtered): 31,770,345 bp (33.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 117468 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 3 1 18 12 0.0 3 0 0 12 19 249 0.0 3 0 241 6 2 20 115112 0.0 4 113252 1783 68 5 4 21 277 0.0 4 33 237 6 1 22 19 0.0 4 3 1 14 1 23 101 0.0 4 0 0 3 81 17 24 4 0.0 4 0 0 0 0 4 25 1 0.0 4 0 0 1 26 1 0.0 4 1 27 1 0.0 4 1 28 1 0.0 4 1 30 1 0.0 4 0 0 0 0 1 33 1 0.0 4 1 35 1 0.0 4 1 37 1 0.0 4 1 99 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 120 2 0.0 4 0 0 0 0 2 121 9 0.0 4 0 0 0 0 9 122 847 0.0 4 0 0 0 0 847 123 2 0.0 4 0 0 0 0 2 125 4 0.0 4 0 0 0 0 4 126 2 0.0 4 0 0 0 0 2 127 749 0.0 4 0 0 0 0 749 130 9 0.0 4 1 0 0 0 8 136 1 0.0 4 1 146 1 0.0 4 1 148 1 0.0 4 0 0 0 0 1 149 4 0.0 4 0 0 0 1 3 153 2 0.0 4 0 0 0 0 2 165 1 0.0 4 0 0 0 1 171 1 0.0 4 1 180 1 0.0 4 0 0 0 0 1 181 1 0.0 4 1 185 1 0.0 4 1 186 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 1 218 1 0.0 4 0 0 0 0 1 223 7 0.0 4 0 0 0 0 7 224 2 0.0 4 0 0 0 0 2 228 7 0.0 4 0 0 0 0 7 229 2 0.0 4 0 0 0 0 2 230 2 0.0 4 0 0 0 0 2 231 1 0.0 4 0 0 1 232 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 245 1 0.0 4 0 0 0 0 1 252 1 0.0 4 0 0 0 0 1 254 1 0.0 4 0 0 0 0 1 263 2 0.0 4 0 0 0 0 2 264 1 0.0 4 0 0 0 0 1 280 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 287 1 0.0 4 0 0 0 0 1 293 2 0.0 4 0 0 0 0 2 294 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.32 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 117,426 Reads with adapters: 29,352 (25.0%) Reads written (passing filters): 117,426 (100.0%) Total basepairs processed: 31,770,345 bp Total written (filtered): 31,493,709 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 29352 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.6% C: 2.3% G: 96.2% T: 0.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 61 1834.8 0 61 4 29 458.7 0 29 5 12 114.7 0 12 6 5 28.7 0 5 7 1 7.2 0 1 8 4 1.8 0 4 9 21964 0.4 0 707 21257 10 5782 0.1 1 203 5579 11 1422 0.0 1 10 1412 12 35 0.0 1 0 35 13 6 0.0 1 1 5 15 1 0.0 1 0 1 38 1 0.0 1 1 40 1 0.0 1 1 43 1 0.0 1 0 1 84 1 0.0 1 1 89 1 0.0 1 1 95 1 0.0 1 0 1 116 1 0.0 1 1 147 1 0.0 1 1 150 10 0.0 1 0 10 152 1 0.0 1 0 1 161 1 0.0 1 1 175 1 0.0 1 1 189 1 0.0 1 0 1 208 5 0.0 1 2 3 210 1 0.0 1 1 220 1 0.0 1 0 1 258 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.