This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TATACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.06 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 43,425 (1.8%) Reads that were too short: 10,512 (0.4%) Reads written (passing filters): 32,913 (1.3%) Total basepairs processed: 740,966,583 bp Total written (filtered): 8,414,620 bp (1.1%) === Adapter 1 === Sequence: TATACC; Type: regular 5'; Length: 6; Trimmed: 43425 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2885 601.0 0 2885 7 4939 601.0 0 4939 8 135 601.0 0 135 9 3763 601.0 0 3763 10 281 601.0 0 281 11 9675 601.0 0 9675 12 42 601.0 0 42 13 43 601.0 0 43 14 31 601.0 0 31 15 31 601.0 0 31 16 36 601.0 0 36 17 39 601.0 0 39 18 28 601.0 0 28 19 35 601.0 0 35 20 47 601.0 0 47 21 55 601.0 0 55 22 47 601.0 0 47 23 52 601.0 0 52 24 31 601.0 0 31 25 38 601.0 0 38 26 35 601.0 0 35 27 44 601.0 0 44 28 29 601.0 0 29 29 29 601.0 0 29 30 37 601.0 0 37 31 44 601.0 0 44 32 35 601.0 0 35 33 32 601.0 0 32 34 39 601.0 0 39 35 38 601.0 0 38 36 33 601.0 0 33 37 38 601.0 0 38 38 36 601.0 0 36 39 30 601.0 0 30 40 30 601.0 0 30 41 35 601.0 0 35 42 31 601.0 0 31 43 38 601.0 0 38 44 34 601.0 0 34 45 32 601.0 0 32 46 34 601.0 0 34 47 42 601.0 0 42 48 43 601.0 0 43 49 47 601.0 0 47 50 38 601.0 0 38 51 35 601.0 0 35 52 90 601.0 0 90 53 46 601.0 0 46 54 38 601.0 0 38 55 52 601.0 0 52 56 56 601.0 0 56 57 31 601.0 0 31 58 35 601.0 0 35 59 48 601.0 0 48 60 32 601.0 0 32 61 38 601.0 0 38 62 28 601.0 0 28 63 41 601.0 0 41 64 46 601.0 0 46 65 56 601.0 0 56 66 42 601.0 0 42 67 47 601.0 0 47 68 40 601.0 0 40 69 44 601.0 0 44 70 44 601.0 0 44 71 54 601.0 0 54 72 99 601.0 0 99 73 241 601.0 0 241 74 44 601.0 0 44 75 176 601.0 0 176 76 44 601.0 0 44 77 33 601.0 0 33 78 24 601.0 0 24 79 42 601.0 0 42 80 30 601.0 0 30 81 36 601.0 0 36 82 35 601.0 0 35 83 28 601.0 0 28 84 35 601.0 0 35 85 32 601.0 0 32 86 33 601.0 0 33 87 48 601.0 0 48 88 46 601.0 0 46 89 48 601.0 0 48 90 46 601.0 0 46 91 40 601.0 0 40 92 39 601.0 0 39 93 35 601.0 0 35 94 30 601.0 0 30 95 47 601.0 0 47 96 37 601.0 0 37 97 25 601.0 0 25 98 37 601.0 0 37 99 47 601.0 0 47 100 47 601.0 0 47 101 224 601.0 0 224 102 135 601.0 0 135 103 160 601.0 0 160 104 44 601.0 0 44 105 101 601.0 0 101 106 61 601.0 0 61 107 26 601.0 0 26 108 44 601.0 0 44 109 39 601.0 0 39 110 30 601.0 0 30 111 86 601.0 0 86 112 124 601.0 0 124 113 127 601.0 0 127 114 108 601.0 0 108 115 65 601.0 0 65 116 39 601.0 0 39 117 62 601.0 0 62 118 85 601.0 0 85 119 44 601.0 0 44 120 36 601.0 0 36 121 43 601.0 0 43 122 33 601.0 0 33 123 51 601.0 0 51 124 58 601.0 0 58 125 54 601.0 0 54 126 40 601.0 0 40 127 43 601.0 0 43 128 37 601.0 0 37 129 32 601.0 0 32 130 43 601.0 0 43 131 67 601.0 0 67 132 156 601.0 0 156 133 147 601.0 0 147 134 175 601.0 0 175 135 41 601.0 0 41 136 49 601.0 0 49 137 158 601.0 0 158 138 113 601.0 0 113 139 125 601.0 0 125 140 36 601.0 0 36 141 77 601.0 0 77 142 56 601.0 0 56 143 58 601.0 0 58 144 156 601.0 0 156 145 186 601.0 0 186 146 460 601.0 0 460 147 34 601.0 0 34 148 46 601.0 0 46 149 154 601.0 0 154 150 69 601.0 0 69 151 95 601.0 0 95 152 47 601.0 0 47 153 30 601.0 0 30 154 58 601.0 0 58 155 70 601.0 0 70 156 96 601.0 0 96 157 30 601.0 0 30 158 67 601.0 0 67 159 57 601.0 0 57 160 55 601.0 0 55 161 52 601.0 0 52 162 39 601.0 0 39 163 38 601.0 0 38 164 49 601.0 0 49 165 42 601.0 0 42 166 55 601.0 0 55 167 38 601.0 0 38 168 52 601.0 0 52 169 67 601.0 0 67 170 161 601.0 0 161 171 178 601.0 0 178 172 195 601.0 0 195 173 39 601.0 0 39 174 24 601.0 0 24 175 35 601.0 0 35 176 37 601.0 0 37 177 58 601.0 0 58 178 75 601.0 0 75 179 94 601.0 0 94 180 87 601.0 0 87 181 42 601.0 0 42 182 36 601.0 0 36 183 30 601.0 0 30 184 34 601.0 0 34 185 45 601.0 0 45 186 38 601.0 0 38 187 50 601.0 0 50 188 41 601.0 0 41 189 58 601.0 0 58 190 43 601.0 0 43 191 34 601.0 0 34 192 40 601.0 0 40 193 36 601.0 0 36 194 38 601.0 0 38 195 44 601.0 0 44 196 39 601.0 0 39 197 40 601.0 0 40 198 57 601.0 0 57 199 53 601.0 0 53 200 38 601.0 0 38 201 37 601.0 0 37 202 42 601.0 0 42 203 38 601.0 0 38 204 61 601.0 0 61 205 63 601.0 0 63 206 41 601.0 0 41 207 35 601.0 0 35 208 38 601.0 0 38 209 52 601.0 0 52 210 111 601.0 0 111 211 117 601.0 0 117 212 219 601.0 0 219 213 53 601.0 0 53 214 62 601.0 0 62 215 38 601.0 0 38 216 55 601.0 0 55 217 43 601.0 0 43 218 55 601.0 0 55 219 58 601.0 0 58 220 53 601.0 0 53 221 34 601.0 0 34 222 35 601.0 0 35 223 56 601.0 0 56 224 75 601.0 0 75 225 104 601.0 0 104 226 776 601.0 0 776 227 654 601.0 0 654 228 535 601.0 0 535 229 73 601.0 0 73 230 31 601.0 0 31 231 42 601.0 0 42 232 40 601.0 0 40 233 74 601.0 0 74 234 44 601.0 0 44 235 48 601.0 0 48 236 56 601.0 0 56 237 56 601.0 0 56 238 42 601.0 0 42 239 40 601.0 0 40 240 62 601.0 0 62 241 38 601.0 0 38 242 43 601.0 0 43 243 52 601.0 0 52 244 53 601.0 0 53 245 87 601.0 0 87 246 91 601.0 0 91 247 167 601.0 0 167 248 114 601.0 0 114 249 73 601.0 0 73 250 63 601.0 0 63 251 49 601.0 0 49 252 35 601.0 0 35 253 54 601.0 0 54 254 49 601.0 0 49 255 70 601.0 0 70 256 86 601.0 0 86 257 86 601.0 0 86 258 100 601.0 0 100 259 97 601.0 0 97 260 157 601.0 0 157 261 70 601.0 0 70 262 72 601.0 0 72 263 55 601.0 0 55 264 93 601.0 0 93 265 102 601.0 0 102 266 168 601.0 0 168 267 57 601.0 0 57 268 53 601.0 0 53 269 60 601.0 0 60 270 48 601.0 0 48 271 59 601.0 0 59 272 122 601.0 0 122 273 103 601.0 0 103 274 77 601.0 0 77 275 67 601.0 0 67 276 70 601.0 0 70 277 160 601.0 0 160 278 77 601.0 0 77 279 63 601.0 0 63 280 105 601.0 0 105 281 95 601.0 0 95 282 98 601.0 0 98 283 86 601.0 0 86 284 290 601.0 0 290 285 474 601.0 0 474 286 679 601.0 0 679 287 448 601.0 0 448 288 106 601.0 0 106 289 70 601.0 0 70 290 48 601.0 0 48 291 64 601.0 0 64 292 91 601.0 0 91 293 92 601.0 0 92 294 39 601.0 0 39 295 62 601.0 0 62 296 69 601.0 0 69 297 53 601.0 0 53 298 65 601.0 0 65 299 77 601.0 0 77 300 59 601.0 0 59 301 91 601.0 0 91 Finished in 1.72 s (52 us/read; 1.15 M reads/minute). === Summary === Total reads processed: 32,913 Reads with adapters: 7,852 (23.9%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 7,846 (23.8%) Total basepairs processed: 8,414,620 bp Total written (filtered): 2,111,559 bp (25.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 7852 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 22 0.0 3 0 21 1 20 4812 0.0 4 4742 63 3 3 1 21 7 0.0 4 1 5 1 22 15 0.0 4 1 13 1 23 2678 0.0 4 2624 51 2 1 24 8 0.0 4 1 7 25 1 0.0 4 1 26 1 0.0 4 0 0 0 1 102 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 1 124 5 0.0 4 0 0 0 0 5 125 283 0.0 4 0 0 0 0 283 127 13 0.0 4 0 0 0 0 13 145 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 226 2 0.0 4 0 0 0 0 2 289 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.27 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 7,846 Reads with adapters: 741 (9.4%) Reads written (passing filters): 7,846 (100.0%) Total basepairs processed: 2,111,559 bp Total written (filtered): 2,104,198 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 741 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.3% C: 3.2% G: 93.3% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 15 122.6 0 15 4 2 30.6 0 2 5 1 7.7 0 1 7 1 0.5 0 1 8 1 0.1 0 1 9 509 0.0 0 22 487 10 129 0.0 1 4 125 11 67 0.0 1 0 67 12 12 0.0 1 0 12 13 1 0.0 1 0 1 140 1 0.0 1 1 175 1 0.0 1 1 208 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.