This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.72 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 206,041 (8.4%) Reads that were too short: 101,435 (4.1%) Reads written (passing filters): 104,606 (4.2%) Total basepairs processed: 740,966,583 bp Total written (filtered): 27,204,836 bp (3.7%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 206041 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 646 601.0 0 646 7 1345 601.0 0 1345 8 37382 601.0 0 37382 9 32481 601.0 0 32481 10 47 601.0 0 47 11 36 601.0 0 36 12 46 601.0 0 46 13 78 601.0 0 78 14 22 601.0 0 22 15 29 601.0 0 29 16 32 601.0 0 32 17 17 601.0 0 17 18 17 601.0 0 17 19 20 601.0 0 20 20 30 601.0 0 30 21 15 601.0 0 15 22 36 601.0 0 36 23 31 601.0 0 31 24 29 601.0 0 29 25 31 601.0 0 31 26 35 601.0 0 35 27 31 601.0 0 31 28 57 601.0 0 57 29 20 601.0 0 20 30 24 601.0 0 24 31 24 601.0 0 24 32 22 601.0 0 22 33 34 601.0 0 34 34 26 601.0 0 26 35 26 601.0 0 26 36 91 601.0 0 91 37 36 601.0 0 36 38 36 601.0 0 36 39 24 601.0 0 24 40 24 601.0 0 24 41 27 601.0 0 27 42 43 601.0 0 43 43 113 601.0 0 113 44 455 601.0 0 455 45 41 601.0 0 41 46 39 601.0 0 39 47 39 601.0 0 39 48 32 601.0 0 32 49 57 601.0 0 57 50 52 601.0 0 52 51 33 601.0 0 33 52 25 601.0 0 25 53 22 601.0 0 22 54 40 601.0 0 40 55 45 601.0 0 45 56 193 601.0 0 193 57 165 601.0 0 165 58 207 601.0 0 207 59 46 601.0 0 46 60 40 601.0 0 40 61 98 601.0 0 98 62 43 601.0 0 43 63 42 601.0 0 42 64 84 601.0 0 84 65 63 601.0 0 63 66 19 601.0 0 19 67 56 601.0 0 56 68 69 601.0 0 69 69 277 601.0 0 277 70 325 601.0 0 325 71 1443 601.0 0 1443 72 77 601.0 0 77 73 64 601.0 0 64 74 59 601.0 0 59 75 78 601.0 0 78 76 143 601.0 0 143 77 60 601.0 0 60 78 87 601.0 0 87 79 73 601.0 0 73 80 76 601.0 0 76 81 87 601.0 0 87 82 81 601.0 0 81 83 75 601.0 0 75 84 57 601.0 0 57 85 83 601.0 0 83 86 112 601.0 0 112 87 151 601.0 0 151 88 194 601.0 0 194 89 3594 601.0 0 3594 90 7113 601.0 0 7113 91 1992 601.0 0 1992 92 51 601.0 0 51 93 42 601.0 0 42 94 31 601.0 0 31 95 38 601.0 0 38 96 36 601.0 0 36 97 192 601.0 0 192 98 148 601.0 0 148 99 79 601.0 0 79 100 96 601.0 0 96 101 62 601.0 0 62 102 103 601.0 0 103 103 42 601.0 0 42 104 33 601.0 0 33 105 46 601.0 0 46 106 35 601.0 0 35 107 58 601.0 0 58 108 59 601.0 0 59 109 42 601.0 0 42 110 78 601.0 0 78 111 75 601.0 0 75 112 45 601.0 0 45 113 37 601.0 0 37 114 20 601.0 0 20 115 19 601.0 0 19 116 21 601.0 0 21 117 44 601.0 0 44 118 30 601.0 0 30 119 40 601.0 0 40 120 39 601.0 0 39 121 41 601.0 0 41 122 35 601.0 0 35 123 35 601.0 0 35 124 37 601.0 0 37 125 50 601.0 0 50 126 37 601.0 0 37 127 46 601.0 0 46 128 44 601.0 0 44 129 57 601.0 0 57 130 37 601.0 0 37 131 47 601.0 0 47 132 58 601.0 0 58 133 97 601.0 0 97 134 81 601.0 0 81 135 33 601.0 0 33 136 38 601.0 0 38 137 166 601.0 0 166 138 168 601.0 0 168 139 482 601.0 0 482 140 35 601.0 0 35 141 43 601.0 0 43 142 47 601.0 0 47 143 38 601.0 0 38 144 42 601.0 0 42 145 31 601.0 0 31 146 24 601.0 0 24 147 36 601.0 0 36 148 214 601.0 0 214 149 94 601.0 0 94 150 134 601.0 0 134 151 44 601.0 0 44 152 44 601.0 0 44 153 60 601.0 0 60 154 37 601.0 0 37 155 63 601.0 0 63 156 41 601.0 0 41 157 129 601.0 0 129 158 71 601.0 0 71 159 83 601.0 0 83 160 94 601.0 0 94 161 63 601.0 0 63 162 52 601.0 0 52 163 43 601.0 0 43 164 45 601.0 0 45 165 48 601.0 0 48 166 31 601.0 0 31 167 42 601.0 0 42 168 233 601.0 0 233 169 164 601.0 0 164 170 347 601.0 0 347 171 36 601.0 0 36 172 41 601.0 0 41 173 69 601.0 0 69 174 81 601.0 0 81 175 189 601.0 0 189 176 1703 601.0 0 1703 177 1220 601.0 0 1220 178 2995 601.0 0 2995 179 78 601.0 0 78 180 34 601.0 0 34 181 22 601.0 0 22 182 90 601.0 0 90 183 97 601.0 0 97 184 59 601.0 0 59 185 44 601.0 0 44 186 63 601.0 0 63 187 40 601.0 0 40 188 43 601.0 0 43 189 97 601.0 0 97 190 33 601.0 0 33 191 28 601.0 0 28 192 34 601.0 0 34 193 43 601.0 0 43 194 47 601.0 0 47 195 40 601.0 0 40 196 55 601.0 0 55 197 82 601.0 0 82 198 57 601.0 0 57 199 44 601.0 0 44 200 35 601.0 0 35 201 36 601.0 0 36 202 111 601.0 0 111 203 154 601.0 0 154 204 106 601.0 0 106 205 99 601.0 0 99 206 55 601.0 0 55 207 113 601.0 0 113 208 100 601.0 0 100 209 186 601.0 0 186 210 310 601.0 0 310 211 234 601.0 0 234 212 94 601.0 0 94 213 84 601.0 0 84 214 79 601.0 0 79 215 91 601.0 0 91 216 103 601.0 0 103 217 110 601.0 0 110 218 117 601.0 0 117 219 338 601.0 0 338 220 2447 601.0 0 2447 221 2307 601.0 0 2307 222 3234 601.0 0 3234 223 165 601.0 0 165 224 51 601.0 0 51 225 79 601.0 0 79 226 180 601.0 0 180 227 55 601.0 0 55 228 42 601.0 0 42 229 30 601.0 0 30 230 167 601.0 0 167 231 126 601.0 0 126 232 202 601.0 0 202 233 82 601.0 0 82 234 70 601.0 0 70 235 88 601.0 0 88 236 51 601.0 0 51 237 102 601.0 0 102 238 76 601.0 0 76 239 106 601.0 0 106 240 138 601.0 0 138 241 122 601.0 0 122 242 117 601.0 0 117 243 132 601.0 0 132 244 336 601.0 0 336 245 197 601.0 0 197 246 132 601.0 0 132 247 71 601.0 0 71 248 66 601.0 0 66 249 86 601.0 0 86 250 93 601.0 0 93 251 82 601.0 0 82 252 51 601.0 0 51 253 125 601.0 0 125 254 89 601.0 0 89 255 204 601.0 0 204 256 149 601.0 0 149 257 254 601.0 0 254 258 57 601.0 0 57 259 106 601.0 0 106 260 109 601.0 0 109 261 101 601.0 0 101 262 338 601.0 0 338 263 389 601.0 0 389 264 302 601.0 0 302 265 226 601.0 0 226 266 202 601.0 0 202 267 123 601.0 0 123 268 509 601.0 0 509 269 1300 601.0 0 1300 270 771 601.0 0 771 271 520 601.0 0 520 272 186 601.0 0 186 273 116 601.0 0 116 274 1103 601.0 0 1103 275 349 601.0 0 349 276 433 601.0 0 433 277 199 601.0 0 199 278 285 601.0 0 285 279 351 601.0 0 351 280 592 601.0 0 592 281 1090 601.0 0 1090 282 2924 601.0 0 2924 283 18778 601.0 0 18778 284 21365 601.0 0 21365 285 27788 601.0 0 27788 286 4656 601.0 0 4656 287 69 601.0 0 69 288 185 601.0 0 185 289 350 601.0 0 350 290 237 601.0 0 237 291 100 601.0 0 100 292 79 601.0 0 79 293 34 601.0 0 34 294 36 601.0 0 36 295 54 601.0 0 54 296 82 601.0 0 82 297 125 601.0 0 125 298 103 601.0 0 103 299 54 601.0 0 54 300 47 601.0 0 47 301 24 601.0 0 24 Finished in 4.94 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 104,606 Reads with adapters: 38,402 (36.7%) Reads that were too short: 133 (0.1%) Reads written (passing filters): 38,269 (36.6%) Total basepairs processed: 27,204,836 bp Total written (filtered): 10,539,930 bp (38.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 38402 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 16 0.0 2 0 0 0 16 15 35 0.0 3 0 0 18 17 16 354 0.0 3 0 290 40 24 17 37379 0.0 3 34347 2420 494 118 18 92 0.0 3 6 61 13 12 19 153 0.0 3 0 1 89 63 20 87 0.0 3 0 0 0 87 22 1 0.0 3 0 0 0 1 26 1 0.0 3 0 0 0 1 28 1 0.0 3 1 29 1 0.0 3 0 0 0 1 32 2 0.0 3 0 0 0 2 40 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 18 0.0 3 0 0 0 18 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 1 49 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 3 0.0 3 0 0 0 3 66 1 0.0 3 0 0 0 1 69 3 0.0 3 0 0 0 3 72 1 0.0 3 0 0 0 1 74 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 1 77 15 0.0 3 0 0 0 15 80 2 0.0 3 0 0 0 2 82 3 0.0 3 0 0 0 3 84 2 0.0 3 0 0 0 2 90 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 100 2 0.0 3 0 0 0 2 101 18 0.0 3 0 0 1 17 102 31 0.0 3 0 0 0 31 103 24 0.0 3 0 0 0 24 104 1 0.0 3 0 0 0 1 105 6 0.0 3 0 0 0 6 106 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 6 0.0 3 0 0 0 6 115 2 0.0 3 0 0 1 1 118 1 0.0 3 0 0 0 1 119 2 0.0 3 0 0 0 2 120 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 128 13 0.0 3 0 0 0 13 132 1 0.0 3 0 0 0 1 133 2 0.0 3 0 0 0 2 135 2 0.0 3 0 0 0 2 136 5 0.0 3 0 0 0 5 137 5 0.0 3 0 0 0 5 138 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 155 1 0.0 3 1 156 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 164 3 0.0 3 0 0 0 3 173 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 177 2 0.0 3 0 0 0 2 178 8 0.0 3 0 0 0 8 179 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 194 4 0.0 3 0 0 0 4 196 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 203 7 0.0 3 0 0 1 6 204 3 0.0 3 0 0 0 3 205 1 0.0 3 0 0 0 1 207 2 0.0 3 0 0 0 2 208 1 0.0 3 0 0 0 1 209 2 0.0 3 0 0 0 2 211 2 0.0 3 0 0 0 2 218 2 0.0 3 0 0 0 2 227 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 0 1 279 6 0.0 3 0 0 0 6 280 7 0.0 3 0 0 0 7 281 6 0.0 3 0 0 0 6 282 2 0.0 3 0 0 1 1 283 2 0.0 3 0 0 0 2 289 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.34 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 38,269 Reads with adapters: 566 (1.5%) Reads written (passing filters): 38,269 (100.0%) Total basepairs processed: 10,539,930 bp Total written (filtered): 10,538,130 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 566 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 58.7% C: 13.1% G: 23.5% T: 4.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 479 598.0 0 479 4 79 149.5 0 79 5 6 37.4 0 6 6 1 9.3 0 1 11 1 0.0 1 0 1