This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.48 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 189,112 (7.7%) Reads that were too short: 81,493 (3.3%) Reads written (passing filters): 107,619 (4.4%) Total basepairs processed: 740,966,583 bp Total written (filtered): 28,111,125 bp (3.8%) === Adapter 1 === Sequence: GTCTTA; Type: regular 5'; Length: 6; Trimmed: 189112 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 589 601.0 0 589 7 971 601.0 0 971 8 34793 601.0 0 34793 9 40488 601.0 0 40488 10 48 601.0 0 48 11 19 601.0 0 19 12 13 601.0 0 13 13 37 601.0 0 37 14 9 601.0 0 9 15 18 601.0 0 18 16 14 601.0 0 14 17 8 601.0 0 8 18 7 601.0 0 7 19 11 601.0 0 11 20 9 601.0 0 9 21 24 601.0 0 24 22 10 601.0 0 10 23 9 601.0 0 9 24 14 601.0 0 14 25 12 601.0 0 12 26 13 601.0 0 13 27 13 601.0 0 13 28 22 601.0 0 22 29 15 601.0 0 15 30 12 601.0 0 12 31 16 601.0 0 16 32 11 601.0 0 11 33 11 601.0 0 11 34 10 601.0 0 10 35 16 601.0 0 16 36 27 601.0 0 27 37 19 601.0 0 19 38 35 601.0 0 35 39 16 601.0 0 16 40 16 601.0 0 16 41 14 601.0 0 14 42 19 601.0 0 19 43 121 601.0 0 121 44 458 601.0 0 458 45 46 601.0 0 46 46 19 601.0 0 19 47 20 601.0 0 20 48 21 601.0 0 21 49 30 601.0 0 30 50 33 601.0 0 33 51 22 601.0 0 22 52 12 601.0 0 12 53 9 601.0 0 9 54 22 601.0 0 22 55 19 601.0 0 19 56 37 601.0 0 37 57 57 601.0 0 57 58 388 601.0 0 388 59 68 601.0 0 68 60 18 601.0 0 18 61 48 601.0 0 48 62 28 601.0 0 28 63 26 601.0 0 26 64 46 601.0 0 46 65 31 601.0 0 31 66 12 601.0 0 12 67 30 601.0 0 30 68 31 601.0 0 31 69 75 601.0 0 75 70 85 601.0 0 85 71 389 601.0 0 389 72 27 601.0 0 27 73 35 601.0 0 35 74 37 601.0 0 37 75 74 601.0 0 74 76 124 601.0 0 124 77 37 601.0 0 37 78 53 601.0 0 53 79 51 601.0 0 51 80 49 601.0 0 49 81 64 601.0 0 64 82 65 601.0 0 65 83 52 601.0 0 52 84 44 601.0 0 44 85 70 601.0 0 70 86 81 601.0 0 81 87 125 601.0 0 125 88 147 601.0 0 147 89 3945 601.0 0 3945 90 8311 601.0 0 8311 91 2058 601.0 0 2058 92 26 601.0 0 26 93 17 601.0 0 17 94 18 601.0 0 18 95 14 601.0 0 14 96 20 601.0 0 20 97 40 601.0 0 40 98 46 601.0 0 46 99 32 601.0 0 32 100 31 601.0 0 31 101 27 601.0 0 27 102 27 601.0 0 27 103 25 601.0 0 25 104 18 601.0 0 18 105 32 601.0 0 32 106 17 601.0 0 17 107 24 601.0 0 24 108 27 601.0 0 27 109 11 601.0 0 11 110 53 601.0 0 53 111 25 601.0 0 25 112 21 601.0 0 21 113 11 601.0 0 11 114 8 601.0 0 8 115 6 601.0 0 6 116 12 601.0 0 12 117 17 601.0 0 17 118 22 601.0 0 22 119 14 601.0 0 14 120 12 601.0 0 12 121 18 601.0 0 18 122 17 601.0 0 17 123 16 601.0 0 16 124 24 601.0 0 24 125 19 601.0 0 19 126 15 601.0 0 15 127 12 601.0 0 12 128 35 601.0 0 35 129 46 601.0 0 46 130 17 601.0 0 17 131 46 601.0 0 46 132 20 601.0 0 20 133 63 601.0 0 63 134 55 601.0 0 55 135 13 601.0 0 13 136 20 601.0 0 20 137 144 601.0 0 144 138 155 601.0 0 155 139 471 601.0 0 471 140 17 601.0 0 17 141 9 601.0 0 9 142 17 601.0 0 17 143 13 601.0 0 13 144 14 601.0 0 14 145 23 601.0 0 23 146 16 601.0 0 16 147 11 601.0 0 11 148 71 601.0 0 71 149 53 601.0 0 53 150 32 601.0 0 32 151 29 601.0 0 29 152 13 601.0 0 13 153 25 601.0 0 25 154 21 601.0 0 21 155 43 601.0 0 43 156 30 601.0 0 30 157 59 601.0 0 59 158 28 601.0 0 28 159 51 601.0 0 51 160 40 601.0 0 40 161 32 601.0 0 32 162 28 601.0 0 28 163 32 601.0 0 32 164 32 601.0 0 32 165 33 601.0 0 33 166 36 601.0 0 36 167 33 601.0 0 33 168 320 601.0 0 320 169 201 601.0 0 201 170 482 601.0 0 482 171 37 601.0 0 37 172 49 601.0 0 49 173 73 601.0 0 73 174 97 601.0 0 97 175 148 601.0 0 148 176 2358 601.0 0 2358 177 1480 601.0 0 1480 178 4013 601.0 0 4013 179 43 601.0 0 43 180 19 601.0 0 19 181 6 601.0 0 6 182 26 601.0 0 26 183 35 601.0 0 35 184 34 601.0 0 34 185 26 601.0 0 26 186 44 601.0 0 44 187 30 601.0 0 30 188 22 601.0 0 22 189 86 601.0 0 86 190 33 601.0 0 33 191 17 601.0 0 17 192 21 601.0 0 21 193 19 601.0 0 19 194 24 601.0 0 24 195 20 601.0 0 20 196 17 601.0 0 17 197 16 601.0 0 16 198 28 601.0 0 28 199 18 601.0 0 18 200 22 601.0 0 22 201 17 601.0 0 17 202 51 601.0 0 51 203 84 601.0 0 84 204 58 601.0 0 58 205 48 601.0 0 48 206 19 601.0 0 19 207 55 601.0 0 55 208 78 601.0 0 78 209 82 601.0 0 82 210 145 601.0 0 145 211 54 601.0 0 54 212 41 601.0 0 41 213 32 601.0 0 32 214 31 601.0 0 31 215 58 601.0 0 58 216 75 601.0 0 75 217 85 601.0 0 85 218 113 601.0 0 113 219 278 601.0 0 278 220 2781 601.0 0 2781 221 2560 601.0 0 2560 222 2776 601.0 0 2776 223 106 601.0 0 106 224 28 601.0 0 28 225 28 601.0 0 28 226 184 601.0 0 184 227 35 601.0 0 35 228 27 601.0 0 27 229 26 601.0 0 26 230 61 601.0 0 61 231 63 601.0 0 63 232 164 601.0 0 164 233 50 601.0 0 50 234 49 601.0 0 49 235 51 601.0 0 51 236 30 601.0 0 30 237 38 601.0 0 38 238 54 601.0 0 54 239 64 601.0 0 64 240 54 601.0 0 54 241 78 601.0 0 78 242 51 601.0 0 51 243 55 601.0 0 55 244 193 601.0 0 193 245 102 601.0 0 102 246 77 601.0 0 77 247 43 601.0 0 43 248 35 601.0 0 35 249 42 601.0 0 42 250 35 601.0 0 35 251 64 601.0 0 64 252 38 601.0 0 38 253 85 601.0 0 85 254 58 601.0 0 58 255 172 601.0 0 172 256 116 601.0 0 116 257 243 601.0 0 243 258 34 601.0 0 34 259 50 601.0 0 50 260 54 601.0 0 54 261 65 601.0 0 65 262 113 601.0 0 113 263 109 601.0 0 109 264 156 601.0 0 156 265 87 601.0 0 87 266 181 601.0 0 181 267 77 601.0 0 77 268 211 601.0 0 211 269 567 601.0 0 567 270 456 601.0 0 456 271 291 601.0 0 291 272 143 601.0 0 143 273 131 601.0 0 131 274 1802 601.0 0 1802 275 512 601.0 0 512 276 535 601.0 0 535 277 154 601.0 0 154 278 222 601.0 0 222 279 258 601.0 0 258 280 392 601.0 0 392 281 1069 601.0 0 1069 282 2222 601.0 0 2222 283 15096 601.0 0 15096 284 15689 601.0 0 15689 285 23478 601.0 0 23478 286 4363 601.0 0 4363 287 51 601.0 0 51 288 68 601.0 0 68 289 136 601.0 0 136 290 104 601.0 0 104 291 70 601.0 0 70 292 96 601.0 0 96 293 29 601.0 0 29 294 39 601.0 0 39 295 31 601.0 0 31 296 27 601.0 0 27 297 73 601.0 0 73 298 81 601.0 0 81 299 37 601.0 0 37 300 20 601.0 0 20 301 11 601.0 0 11 Finished in 5.43 s (50 us/read; 1.19 M reads/minute). === Summary === Total reads processed: 107,619 Reads with adapters: 41,779 (38.8%) Reads that were too short: 24 (0.0%) Reads written (passing filters): 41,755 (38.8%) Total basepairs processed: 28,111,125 bp Total written (filtered): 11,340,620 bp (40.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 41779 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 4 0.0 3 0 0 4 19 129 0.0 3 0 128 0 1 20 41318 0.0 4 40654 636 26 2 21 89 0.0 4 20 68 0 0 1 22 10 0.0 4 0 2 8 23 28 0.0 4 0 0 0 27 1 24 3 0.0 4 1 1 0 0 1 78 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 1 86 1 0.0 4 0 1 97 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 105 1 0.0 4 1 110 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 167 0.0 4 0 0 0 0 167 128 1 0.0 4 0 0 0 0 1 131 1 0.0 4 0 0 0 0 1 146 3 0.0 4 0 0 0 0 3 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 175 1 0.0 4 0 0 0 0 1 198 1 0.0 4 1 210 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 234 2 0.0 4 0 0 0 0 2 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.54 s (37 us/read; 1.63 M reads/minute). === Summary === Total reads processed: 41,755 Reads with adapters: 8,640 (20.7%) Reads written (passing filters): 41,755 (100.0%) Total basepairs processed: 11,340,620 bp Total written (filtered): 11,258,310 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 8640 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.9% C: 2.4% G: 95.5% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 14 652.4 0 14 4 10 163.1 0 10 5 3 40.8 0 3 6 2 10.2 0 2 7 2 2.5 0 2 8 4 0.6 0 4 9 5848 0.2 0 176 5672 10 2066 0.0 1 73 1993 11 672 0.0 1 7 665 12 7 0.0 1 0 7 13 2 0.0 1 0 2 14 1 0.0 1 0 1 70 1 0.0 1 0 1 150 6 0.0 1 0 6 169 1 0.0 1 1 208 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.