This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 64.30 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 110,238 (4.5%) Reads that were too short: 24,391 (1.0%) Reads written (passing filters): 85,847 (3.5%) Total basepairs processed: 740,966,583 bp Total written (filtered): 17,090,862 bp (2.3%) === Adapter 1 === Sequence: AGGATG; Type: regular 5'; Length: 6; Trimmed: 110238 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 173 601.0 0 173 7 4105 601.0 0 4105 8 3040 601.0 0 3040 9 87 601.0 0 87 10 66 601.0 0 66 11 85 601.0 0 85 12 60 601.0 0 60 13 61 601.0 0 61 14 73 601.0 0 73 15 69 601.0 0 69 16 70 601.0 0 70 17 88 601.0 0 88 18 192 601.0 0 192 19 114 601.0 0 114 20 89 601.0 0 89 21 90 601.0 0 90 22 70 601.0 0 70 23 62 601.0 0 62 24 59 601.0 0 59 25 65 601.0 0 65 26 77 601.0 0 77 27 66 601.0 0 66 28 99 601.0 0 99 29 71 601.0 0 71 30 125 601.0 0 125 31 72 601.0 0 72 32 68 601.0 0 68 33 105 601.0 0 105 34 229 601.0 0 229 35 78 601.0 0 78 36 88 601.0 0 88 37 93 601.0 0 93 38 95 601.0 0 95 39 74 601.0 0 74 40 88 601.0 0 88 41 61 601.0 0 61 42 94 601.0 0 94 43 85 601.0 0 85 44 42 601.0 0 42 45 76 601.0 0 76 46 135 601.0 0 135 47 77 601.0 0 77 48 85 601.0 0 85 49 158 601.0 0 158 50 87 601.0 0 87 51 120 601.0 0 120 52 82 601.0 0 82 53 89 601.0 0 89 54 79 601.0 0 79 55 81 601.0 0 81 56 122 601.0 0 122 57 71 601.0 0 71 58 117 601.0 0 117 59 105 601.0 0 105 60 90 601.0 0 90 61 104 601.0 0 104 62 80 601.0 0 80 63 76 601.0 0 76 64 101 601.0 0 101 65 119 601.0 0 119 66 226 601.0 0 226 67 420 601.0 0 420 68 164 601.0 0 164 69 214 601.0 0 214 70 252 601.0 0 252 71 147 601.0 0 147 72 189 601.0 0 189 73 207 601.0 0 207 74 166 601.0 0 166 75 206 601.0 0 206 76 248 601.0 0 248 77 366 601.0 0 366 78 416 601.0 0 416 79 1047 601.0 0 1047 80 15367 601.0 0 15367 81 11262 601.0 0 11262 82 12506 601.0 0 12506 83 160 601.0 0 160 84 257 601.0 0 257 85 103 601.0 0 103 86 107 601.0 0 107 87 116 601.0 0 116 88 108 601.0 0 108 89 107 601.0 0 107 90 49 601.0 0 49 91 50 601.0 0 50 92 40 601.0 0 40 93 53 601.0 0 53 94 78 601.0 0 78 95 214 601.0 0 214 96 99 601.0 0 99 97 75 601.0 0 75 98 68 601.0 0 68 99 104 601.0 0 104 100 67 601.0 0 67 101 44 601.0 0 44 102 42 601.0 0 42 103 100 601.0 0 100 104 63 601.0 0 63 105 76 601.0 0 76 106 59 601.0 0 59 107 64 601.0 0 64 108 53 601.0 0 53 109 48 601.0 0 48 110 64 601.0 0 64 111 81 601.0 0 81 112 106 601.0 0 106 113 145 601.0 0 145 114 71 601.0 0 71 115 58 601.0 0 58 116 58 601.0 0 58 117 145 601.0 0 145 118 128 601.0 0 128 119 145 601.0 0 145 120 279 601.0 0 279 121 333 601.0 0 333 122 222 601.0 0 222 123 106 601.0 0 106 124 130 601.0 0 130 125 139 601.0 0 139 126 112 601.0 0 112 127 558 601.0 0 558 128 282 601.0 0 282 129 428 601.0 0 428 130 130 601.0 0 130 131 80 601.0 0 80 132 205 601.0 0 205 133 90 601.0 0 90 134 65 601.0 0 65 135 90 601.0 0 90 136 137 601.0 0 137 137 84 601.0 0 84 138 83 601.0 0 83 139 143 601.0 0 143 140 144 601.0 0 144 141 140 601.0 0 140 142 104 601.0 0 104 143 85 601.0 0 85 144 125 601.0 0 125 145 174 601.0 0 174 146 112 601.0 0 112 147 102 601.0 0 102 148 185 601.0 0 185 149 104 601.0 0 104 150 107 601.0 0 107 151 43 601.0 0 43 152 81 601.0 0 81 153 69 601.0 0 69 154 82 601.0 0 82 155 66 601.0 0 66 156 62 601.0 0 62 157 49 601.0 0 49 158 68 601.0 0 68 159 69 601.0 0 69 160 71 601.0 0 71 161 77 601.0 0 77 162 58 601.0 0 58 163 70 601.0 0 70 164 87 601.0 0 87 165 62 601.0 0 62 166 94 601.0 0 94 167 386 601.0 0 386 168 124 601.0 0 124 169 392 601.0 0 392 170 72 601.0 0 72 171 66 601.0 0 66 172 63 601.0 0 63 173 84 601.0 0 84 174 83 601.0 0 83 175 146 601.0 0 146 176 99 601.0 0 99 177 121 601.0 0 121 178 103 601.0 0 103 179 84 601.0 0 84 180 82 601.0 0 82 181 129 601.0 0 129 182 138 601.0 0 138 183 175 601.0 0 175 184 152 601.0 0 152 185 170 601.0 0 170 186 208 601.0 0 208 187 359 601.0 0 359 188 2692 601.0 0 2692 189 6779 601.0 0 6779 190 4775 601.0 0 4775 191 678 601.0 0 678 192 708 601.0 0 708 193 333 601.0 0 333 194 225 601.0 0 225 195 236 601.0 0 236 196 308 601.0 0 308 197 102 601.0 0 102 198 68 601.0 0 68 199 84 601.0 0 84 200 75 601.0 0 75 201 72 601.0 0 72 202 96 601.0 0 96 203 96 601.0 0 96 204 98 601.0 0 98 205 239 601.0 0 239 206 644 601.0 0 644 207 568 601.0 0 568 208 1954 601.0 0 1954 209 137 601.0 0 137 210 125 601.0 0 125 211 408 601.0 0 408 212 576 601.0 0 576 213 482 601.0 0 482 214 182 601.0 0 182 215 73 601.0 0 73 216 151 601.0 0 151 217 106 601.0 0 106 218 83 601.0 0 83 219 95 601.0 0 95 220 83 601.0 0 83 221 340 601.0 0 340 222 244 601.0 0 244 223 271 601.0 0 271 224 121 601.0 0 121 225 83 601.0 0 83 226 86 601.0 0 86 227 154 601.0 0 154 228 107 601.0 0 107 229 180 601.0 0 180 230 178 601.0 0 178 231 97 601.0 0 97 232 114 601.0 0 114 233 133 601.0 0 133 234 126 601.0 0 126 235 115 601.0 0 115 236 91 601.0 0 91 237 128 601.0 0 128 238 120 601.0 0 120 239 113 601.0 0 113 240 106 601.0 0 106 241 180 601.0 0 180 242 118 601.0 0 118 243 123 601.0 0 123 244 107 601.0 0 107 245 99 601.0 0 99 246 124 601.0 0 124 247 133 601.0 0 133 248 137 601.0 0 137 249 72 601.0 0 72 250 88 601.0 0 88 251 126 601.0 0 126 252 166 601.0 0 166 253 105 601.0 0 105 254 125 601.0 0 125 255 167 601.0 0 167 256 198 601.0 0 198 257 173 601.0 0 173 258 201 601.0 0 201 259 142 601.0 0 142 260 125 601.0 0 125 261 105 601.0 0 105 262 86 601.0 0 86 263 117 601.0 0 117 264 98 601.0 0 98 265 83 601.0 0 83 266 116 601.0 0 116 267 146 601.0 0 146 268 493 601.0 0 493 269 388 601.0 0 388 270 319 601.0 0 319 271 1743 601.0 0 1743 272 1858 601.0 0 1858 273 956 601.0 0 956 274 323 601.0 0 323 275 81 601.0 0 81 276 79 601.0 0 79 277 87 601.0 0 87 278 182 601.0 0 182 279 109 601.0 0 109 280 137 601.0 0 137 281 114 601.0 0 114 282 951 601.0 0 951 283 493 601.0 0 493 284 281 601.0 0 281 285 80 601.0 0 80 286 93 601.0 0 93 287 119 601.0 0 119 288 161 601.0 0 161 289 115 601.0 0 115 290 106 601.0 0 106 291 136 601.0 0 136 292 234 601.0 0 234 293 276 601.0 0 276 294 420 601.0 0 420 295 224 601.0 0 224 296 135 601.0 0 135 297 153 601.0 0 153 298 142 601.0 0 142 299 131 601.0 0 131 300 344 601.0 0 344 301 365 601.0 0 365 Finished in 3.29 s (38 us/read; 1.57 M reads/minute). === Summary === Total reads processed: 85,847 Reads with adapters: 4,731 (5.5%) Reads that were too short: 281 (0.3%) Reads written (passing filters): 4,450 (5.2%) Total basepairs processed: 17,090,862 bp Total written (filtered): 1,153,971 bp (6.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4731 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 7 0.0 3 0 0 2 5 16 37 0.0 3 0 32 3 2 17 3829 0.0 3 3513 243 57 16 18 14 0.0 3 0 9 1 4 19 39 0.0 3 10 9 10 10 20 10 0.0 3 0 0 1 9 21 1 0.0 3 0 0 0 1 22 1 0.0 3 0 0 0 1 23 3 0.0 3 0 0 0 3 24 1 0.0 3 0 0 0 1 27 3 0.0 3 0 0 0 3 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 0 1 32 6 0.0 3 0 0 0 6 33 3 0.0 3 0 0 1 2 35 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 40 4 0.0 3 0 0 0 4 41 1 0.0 3 0 0 0 1 42 9 0.0 3 0 0 0 9 43 4 0.0 3 0 0 0 4 45 2 0.0 3 0 0 0 2 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 52 16 0.0 3 9 4 3 53 2 0.0 3 0 0 0 2 54 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 6 0.0 3 0 0 0 6 64 8 0.0 3 0 0 0 8 65 16 0.0 3 0 0 0 16 66 3 0.0 3 0 0 0 3 67 2 0.0 3 0 0 0 2 68 3 0.0 3 0 0 0 3 69 2 0.0 3 0 0 0 2 70 1 0.0 3 0 0 0 1 71 2 0.0 3 0 0 0 2 72 4 0.0 3 0 0 0 4 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 79 3 0.0 3 0 0 0 3 80 1 0.0 3 0 0 0 1 81 4 0.0 3 0 0 0 4 82 3 0.0 3 0 0 0 3 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 1 85 1 0.0 3 0 0 0 1 86 98 0.0 3 0 0 0 98 87 7 0.0 3 0 0 0 7 88 2 0.0 3 0 0 0 2 89 20 0.0 3 0 0 2 18 90 4 0.0 3 0 0 0 4 91 17 0.0 3 0 0 1 16 92 11 0.0 3 0 0 0 11 93 4 0.0 3 0 0 0 4 94 6 0.0 3 0 0 0 6 95 7 0.0 3 0 0 0 7 97 4 0.0 3 0 0 0 4 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 3 0.0 3 0 0 0 3 101 3 0.0 3 0 0 0 3 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 3 0.0 3 0 0 0 3 105 1 0.0 3 0 0 0 1 106 29 0.0 3 0 0 0 29 107 1 0.0 3 0 0 0 1 108 3 0.0 3 0 0 0 3 109 10 0.0 3 0 0 0 10 110 11 0.0 3 0 0 2 9 111 124 0.0 3 0 0 1 123 112 8 0.0 3 0 0 0 8 113 1 0.0 3 0 0 0 1 114 19 0.0 3 0 0 0 19 115 12 0.0 3 0 0 0 12 117 2 0.0 3 0 0 0 2 118 3 0.0 3 0 0 0 3 123 2 0.0 3 0 0 0 2 126 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 20 0.0 3 0 0 1 19 130 11 0.0 3 0 0 2 9 132 2 0.0 3 0 0 0 2 133 1 0.0 3 0 0 0 1 134 3 0.0 3 0 0 0 3 135 7 0.0 3 0 0 0 7 136 9 0.0 3 0 0 2 7 137 12 0.0 3 0 0 0 12 138 2 0.0 3 0 0 0 2 139 2 0.0 3 0 0 1 1 140 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 0 2 145 3 0.0 3 0 0 0 3 146 7 0.0 3 0 0 0 7 147 3 0.0 3 0 0 0 3 149 3 0.0 3 0 0 0 3 150 1 0.0 3 0 0 0 1 151 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 154 2 0.0 3 0 0 0 2 156 2 0.0 3 0 0 0 2 157 5 0.0 3 0 0 0 5 158 2 0.0 3 0 0 0 2 159 2 0.0 3 0 0 0 2 161 2 0.0 3 0 0 0 2 162 6 0.0 3 0 0 0 6 163 4 0.0 3 0 0 0 4 165 1 0.0 3 0 0 0 1 166 8 0.0 3 0 0 0 8 167 1 0.0 3 0 0 0 1 171 9 0.0 3 0 0 1 8 172 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 176 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 188 2 0.0 3 0 0 0 2 189 2 0.0 3 0 0 0 2 190 1 0.0 3 0 0 0 1 191 12 0.0 3 0 0 0 12 192 1 0.0 3 0 0 0 1 193 3 0.0 3 0 0 0 3 195 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 1 199 1 0.0 3 0 0 0 1 200 2 0.0 3 0 0 0 2 201 1 0.0 3 0 0 0 1 203 2 0.0 3 0 0 0 2 204 4 0.0 3 0 0 0 4 205 1 0.0 3 0 0 0 1 206 3 0.0 3 0 0 0 3 207 6 0.0 3 0 0 0 6 208 1 0.0 3 0 0 0 1 210 3 0.0 3 0 0 0 3 212 7 0.0 3 0 0 0 7 213 11 0.0 3 0 0 2 9 214 1 0.0 3 0 0 1 215 2 0.0 3 0 0 0 2 216 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 6 0.0 3 0 0 0 6 220 3 0.0 3 0 0 0 3 221 2 0.0 3 0 0 0 2 225 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 255 1 0.0 3 0 0 1 261 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.16 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 4,450 Reads with adapters: 24 (0.5%) Reads written (passing filters): 4,450 (100.0%) Total basepairs processed: 1,153,971 bp Total written (filtered): 1,153,899 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 24 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 20.8% C: 29.2% G: 37.5% T: 12.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 24 69.5 0 24