This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGGATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.05 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 94,678 (3.8%) Reads that were too short: 16,506 (0.7%) Reads written (passing filters): 78,172 (3.2%) Total basepairs processed: 740,966,583 bp Total written (filtered): 15,637,514 bp (2.1%) === Adapter 1 === Sequence: AGGATG; Type: regular 5'; Length: 6; Trimmed: 94678 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 97 601.0 0 97 7 3294 601.0 0 3294 8 4115 601.0 0 4115 9 54 601.0 0 54 10 51 601.0 0 51 11 51 601.0 0 51 12 35 601.0 0 35 13 36 601.0 0 36 14 46 601.0 0 46 15 48 601.0 0 48 16 48 601.0 0 48 17 48 601.0 0 48 18 67 601.0 0 67 19 60 601.0 0 60 20 58 601.0 0 58 21 53 601.0 0 53 22 51 601.0 0 51 23 53 601.0 0 53 24 48 601.0 0 48 25 38 601.0 0 38 26 50 601.0 0 50 27 76 601.0 0 76 28 82 601.0 0 82 29 51 601.0 0 51 30 132 601.0 0 132 31 48 601.0 0 48 32 57 601.0 0 57 33 71 601.0 0 71 34 227 601.0 0 227 35 43 601.0 0 43 36 75 601.0 0 75 37 86 601.0 0 86 38 55 601.0 0 55 39 58 601.0 0 58 40 84 601.0 0 84 41 55 601.0 0 55 42 71 601.0 0 71 43 76 601.0 0 76 44 43 601.0 0 43 45 74 601.0 0 74 46 122 601.0 0 122 47 54 601.0 0 54 48 72 601.0 0 72 49 152 601.0 0 152 50 70 601.0 0 70 51 125 601.0 0 125 52 73 601.0 0 73 53 68 601.0 0 68 54 64 601.0 0 64 55 59 601.0 0 59 56 99 601.0 0 99 57 46 601.0 0 46 58 75 601.0 0 75 59 71 601.0 0 71 60 85 601.0 0 85 61 79 601.0 0 79 62 96 601.0 0 96 63 82 601.0 0 82 64 106 601.0 0 106 65 111 601.0 0 111 66 240 601.0 0 240 67 442 601.0 0 442 68 149 601.0 0 149 69 235 601.0 0 235 70 204 601.0 0 204 71 109 601.0 0 109 72 165 601.0 0 165 73 159 601.0 0 159 74 177 601.0 0 177 75 159 601.0 0 159 76 160 601.0 0 160 77 282 601.0 0 282 78 315 601.0 0 315 79 997 601.0 0 997 80 14944 601.0 0 14944 81 10632 601.0 0 10632 82 11806 601.0 0 11806 83 98 601.0 0 98 84 139 601.0 0 139 85 36 601.0 0 36 86 59 601.0 0 59 87 104 601.0 0 104 88 61 601.0 0 61 89 86 601.0 0 86 90 30 601.0 0 30 91 35 601.0 0 35 92 41 601.0 0 41 93 37 601.0 0 37 94 40 601.0 0 40 95 77 601.0 0 77 96 49 601.0 0 49 97 56 601.0 0 56 98 43 601.0 0 43 99 87 601.0 0 87 100 43 601.0 0 43 101 35 601.0 0 35 102 37 601.0 0 37 103 75 601.0 0 75 104 60 601.0 0 60 105 51 601.0 0 51 106 54 601.0 0 54 107 65 601.0 0 65 108 39 601.0 0 39 109 40 601.0 0 40 110 45 601.0 0 45 111 58 601.0 0 58 112 93 601.0 0 93 113 109 601.0 0 109 114 44 601.0 0 44 115 35 601.0 0 35 116 47 601.0 0 47 117 55 601.0 0 55 118 55 601.0 0 55 119 75 601.0 0 75 120 114 601.0 0 114 121 147 601.0 0 147 122 138 601.0 0 138 123 62 601.0 0 62 124 104 601.0 0 104 125 61 601.0 0 61 126 70 601.0 0 70 127 456 601.0 0 456 128 230 601.0 0 230 129 308 601.0 0 308 130 104 601.0 0 104 131 72 601.0 0 72 132 152 601.0 0 152 133 64 601.0 0 64 134 57 601.0 0 57 135 64 601.0 0 64 136 114 601.0 0 114 137 44 601.0 0 44 138 49 601.0 0 49 139 77 601.0 0 77 140 85 601.0 0 85 141 91 601.0 0 91 142 55 601.0 0 55 143 54 601.0 0 54 144 101 601.0 0 101 145 139 601.0 0 139 146 101 601.0 0 101 147 64 601.0 0 64 148 127 601.0 0 127 149 56 601.0 0 56 150 101 601.0 0 101 151 49 601.0 0 49 152 62 601.0 0 62 153 58 601.0 0 58 154 63 601.0 0 63 155 55 601.0 0 55 156 55 601.0 0 55 157 39 601.0 0 39 158 49 601.0 0 49 159 38 601.0 0 38 160 46 601.0 0 46 161 38 601.0 0 38 162 46 601.0 0 46 163 60 601.0 0 60 164 66 601.0 0 66 165 71 601.0 0 71 166 54 601.0 0 54 167 376 601.0 0 376 168 72 601.0 0 72 169 406 601.0 0 406 170 69 601.0 0 69 171 44 601.0 0 44 172 47 601.0 0 47 173 60 601.0 0 60 174 66 601.0 0 66 175 120 601.0 0 120 176 67 601.0 0 67 177 74 601.0 0 74 178 79 601.0 0 79 179 75 601.0 0 75 180 50 601.0 0 50 181 96 601.0 0 96 182 109 601.0 0 109 183 94 601.0 0 94 184 122 601.0 0 122 185 116 601.0 0 116 186 140 601.0 0 140 187 271 601.0 0 271 188 2621 601.0 0 2621 189 7077 601.0 0 7077 190 4911 601.0 0 4911 191 490 601.0 0 490 192 465 601.0 0 465 193 133 601.0 0 133 194 96 601.0 0 96 195 140 601.0 0 140 196 152 601.0 0 152 197 72 601.0 0 72 198 48 601.0 0 48 199 55 601.0 0 55 200 44 601.0 0 44 201 63 601.0 0 63 202 43 601.0 0 43 203 51 601.0 0 51 204 69 601.0 0 69 205 182 601.0 0 182 206 546 601.0 0 546 207 439 601.0 0 439 208 1688 601.0 0 1688 209 63 601.0 0 63 210 79 601.0 0 79 211 146 601.0 0 146 212 182 601.0 0 182 213 187 601.0 0 187 214 123 601.0 0 123 215 65 601.0 0 65 216 165 601.0 0 165 217 113 601.0 0 113 218 84 601.0 0 84 219 98 601.0 0 98 220 51 601.0 0 51 221 373 601.0 0 373 222 199 601.0 0 199 223 290 601.0 0 290 224 105 601.0 0 105 225 65 601.0 0 65 226 50 601.0 0 50 227 119 601.0 0 119 228 53 601.0 0 53 229 70 601.0 0 70 230 69 601.0 0 69 231 73 601.0 0 73 232 49 601.0 0 49 233 46 601.0 0 46 234 50 601.0 0 50 235 52 601.0 0 52 236 43 601.0 0 43 237 59 601.0 0 59 238 42 601.0 0 42 239 76 601.0 0 76 240 44 601.0 0 44 241 68 601.0 0 68 242 56 601.0 0 56 243 76 601.0 0 76 244 73 601.0 0 73 245 56 601.0 0 56 246 46 601.0 0 46 247 75 601.0 0 75 248 87 601.0 0 87 249 60 601.0 0 60 250 55 601.0 0 55 251 73 601.0 0 73 252 87 601.0 0 87 253 56 601.0 0 56 254 84 601.0 0 84 255 88 601.0 0 88 256 95 601.0 0 95 257 82 601.0 0 82 258 111 601.0 0 111 259 84 601.0 0 84 260 68 601.0 0 68 261 85 601.0 0 85 262 64 601.0 0 64 263 78 601.0 0 78 264 57 601.0 0 57 265 50 601.0 0 50 266 58 601.0 0 58 267 87 601.0 0 87 268 197 601.0 0 197 269 170 601.0 0 170 270 183 601.0 0 183 271 1578 601.0 0 1578 272 1736 601.0 0 1736 273 538 601.0 0 538 274 204 601.0 0 204 275 69 601.0 0 69 276 47 601.0 0 47 277 56 601.0 0 56 278 73 601.0 0 73 279 73 601.0 0 73 280 84 601.0 0 84 281 72 601.0 0 72 282 582 601.0 0 582 283 318 601.0 0 318 284 213 601.0 0 213 285 60 601.0 0 60 286 83 601.0 0 83 287 74 601.0 0 74 288 74 601.0 0 74 289 51 601.0 0 51 290 55 601.0 0 55 291 81 601.0 0 81 292 204 601.0 0 204 293 278 601.0 0 278 294 336 601.0 0 336 295 144 601.0 0 144 296 77 601.0 0 77 297 91 601.0 0 91 298 87 601.0 0 87 299 59 601.0 0 59 300 151 601.0 0 151 301 148 601.0 0 148 Finished in 3.42 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 78,172 Reads with adapters: 4,212 (5.4%) Reads that were too short: 54 (0.1%) Reads written (passing filters): 4,158 (5.3%) Total basepairs processed: 15,637,514 bp Total written (filtered): 1,133,858 bp (7.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 4212 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 0 3 18 1 0.0 3 0 0 0 0 1 19 31 0.0 3 0 30 1 20 4092 0.0 4 4023 66 2 1 21 14 0.0 4 2 11 1 22 5 0.0 4 2 0 1 1 1 23 2 0.0 4 0 0 0 2 55 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 1 92 1 0.0 4 0 0 1 94 2 0.0 4 2 96 1 0.0 4 1 104 2 0.0 4 0 0 0 0 2 110 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 2 0.0 4 0 0 0 0 2 128 1 0.0 4 1 129 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 1 140 2 0.0 4 0 0 0 0 2 149 1 0.0 4 0 0 0 0 1 155 25 0.0 4 0 0 0 0 25 156 11 0.0 4 0 0 0 2 9 160 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 1 210 2 0.0 4 0 0 0 0 2 225 3 0.0 4 0 0 0 0 3 228 1 0.0 4 0 0 0 0 1 258 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.15 s (36 us/read; 1.66 M reads/minute). === Summary === Total reads processed: 4,158 Reads with adapters: 473 (11.4%) Reads written (passing filters): 4,158 (100.0%) Total basepairs processed: 1,133,858 bp Total written (filtered): 1,128,742 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 473 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.4% C: 3.0% G: 95.6% T: 1.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 65.0 0 1 4 1 16.2 0 1 9 15 0.0 0 0 15 10 43 0.0 1 1 42 11 412 0.0 1 1 411 12 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.