This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACCATA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.18 s (26 us/read; 2.30 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 154,550 (6.3%) Reads that were too short: 56,815 (2.3%) Reads written (passing filters): 97,735 (4.0%) Total basepairs processed: 740,966,583 bp Total written (filtered): 17,626,228 bp (2.4%) === Adapter 1 === Sequence: ACCATA; Type: regular 5'; Length: 6; Trimmed: 154550 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 252 601.0 0 252 7 5512 601.0 0 5512 8 148 601.0 0 148 9 149 601.0 0 149 10 188 601.0 0 188 11 164 601.0 0 164 12 157 601.0 0 157 13 188 601.0 0 188 14 187 601.0 0 187 15 144 601.0 0 144 16 153 601.0 0 153 17 149 601.0 0 149 18 139 601.0 0 139 19 162 601.0 0 162 20 134 601.0 0 134 21 161 601.0 0 161 22 154 601.0 0 154 23 181 601.0 0 181 24 163 601.0 0 163 25 151 601.0 0 151 26 149 601.0 0 149 27 164 601.0 0 164 28 162 601.0 0 162 29 168 601.0 0 168 30 121 601.0 0 121 31 153 601.0 0 153 32 131 601.0 0 131 33 138 601.0 0 138 34 157 601.0 0 157 35 161 601.0 0 161 36 158 601.0 0 158 37 134 601.0 0 134 38 162 601.0 0 162 39 144 601.0 0 144 40 157 601.0 0 157 41 136 601.0 0 136 42 153 601.0 0 153 43 158 601.0 0 158 44 133 601.0 0 133 45 135 601.0 0 135 46 126 601.0 0 126 47 157 601.0 0 157 48 147 601.0 0 147 49 121 601.0 0 121 50 181 601.0 0 181 51 138 601.0 0 138 52 112 601.0 0 112 53 138 601.0 0 138 54 153 601.0 0 153 55 136 601.0 0 136 56 162 601.0 0 162 57 146 601.0 0 146 58 129 601.0 0 129 59 125 601.0 0 125 60 186 601.0 0 186 61 142 601.0 0 142 62 153 601.0 0 153 63 160 601.0 0 160 64 168 601.0 0 168 65 149 601.0 0 149 66 133 601.0 0 133 67 181 601.0 0 181 68 152 601.0 0 152 69 194 601.0 0 194 70 185 601.0 0 185 71 139 601.0 0 139 72 167 601.0 0 167 73 270 601.0 0 270 74 1459 601.0 0 1459 75 644 601.0 0 644 76 953 601.0 0 953 77 193 601.0 0 193 78 234 601.0 0 234 79 178 601.0 0 178 80 172 601.0 0 172 81 193 601.0 0 193 82 150 601.0 0 150 83 141 601.0 0 141 84 151 601.0 0 151 85 144 601.0 0 144 86 131 601.0 0 131 87 175 601.0 0 175 88 162 601.0 0 162 89 184 601.0 0 184 90 156 601.0 0 156 91 181 601.0 0 181 92 124 601.0 0 124 93 146 601.0 0 146 94 103 601.0 0 103 95 152 601.0 0 152 96 146 601.0 0 146 97 160 601.0 0 160 98 156 601.0 0 156 99 191 601.0 0 191 100 171 601.0 0 171 101 168 601.0 0 168 102 133 601.0 0 133 103 206 601.0 0 206 104 152 601.0 0 152 105 166 601.0 0 166 106 189 601.0 0 189 107 186 601.0 0 186 108 218 601.0 0 218 109 341 601.0 0 341 110 198 601.0 0 198 111 156 601.0 0 156 112 187 601.0 0 187 113 175 601.0 0 175 114 222 601.0 0 222 115 152 601.0 0 152 116 170 601.0 0 170 117 242 601.0 0 242 118 219 601.0 0 219 119 206 601.0 0 206 120 362 601.0 0 362 121 417 601.0 0 417 122 222 601.0 0 222 123 268 601.0 0 268 124 270 601.0 0 270 125 301 601.0 0 301 126 262 601.0 0 262 127 289 601.0 0 289 128 237 601.0 0 237 129 278 601.0 0 278 130 327 601.0 0 327 131 469 601.0 0 469 132 536 601.0 0 536 133 3467 601.0 0 3467 134 17545 601.0 0 17545 135 8033 601.0 0 8033 136 2607 601.0 0 2607 137 344 601.0 0 344 138 245 601.0 0 245 139 250 601.0 0 250 140 1599 601.0 0 1599 141 1712 601.0 0 1712 142 1118 601.0 0 1118 143 153 601.0 0 153 144 159 601.0 0 159 145 203 601.0 0 203 146 174 601.0 0 174 147 278 601.0 0 278 148 247 601.0 0 247 149 421 601.0 0 421 150 184 601.0 0 184 151 198 601.0 0 198 152 231 601.0 0 231 153 275 601.0 0 275 154 1774 601.0 0 1774 155 684 601.0 0 684 156 686 601.0 0 686 157 5905 601.0 0 5905 158 4961 601.0 0 4961 159 7459 601.0 0 7459 160 204 601.0 0 204 161 156 601.0 0 156 162 182 601.0 0 182 163 160 601.0 0 160 164 215 601.0 0 215 165 170 601.0 0 170 166 175 601.0 0 175 167 152 601.0 0 152 168 139 601.0 0 139 169 164 601.0 0 164 170 123 601.0 0 123 171 143 601.0 0 143 172 139 601.0 0 139 173 134 601.0 0 134 174 139 601.0 0 139 175 156 601.0 0 156 176 112 601.0 0 112 177 135 601.0 0 135 178 123 601.0 0 123 179 138 601.0 0 138 180 133 601.0 0 133 181 142 601.0 0 142 182 138 601.0 0 138 183 108 601.0 0 108 184 101 601.0 0 101 185 124 601.0 0 124 186 135 601.0 0 135 187 117 601.0 0 117 188 139 601.0 0 139 189 111 601.0 0 111 190 152 601.0 0 152 191 184 601.0 0 184 192 202 601.0 0 202 193 144 601.0 0 144 194 107 601.0 0 107 195 107 601.0 0 107 196 153 601.0 0 153 197 126 601.0 0 126 198 103 601.0 0 103 199 145 601.0 0 145 200 122 601.0 0 122 201 137 601.0 0 137 202 150 601.0 0 150 203 118 601.0 0 118 204 134 601.0 0 134 205 127 601.0 0 127 206 147 601.0 0 147 207 150 601.0 0 150 208 127 601.0 0 127 209 112 601.0 0 112 210 117 601.0 0 117 211 114 601.0 0 114 212 120 601.0 0 120 213 136 601.0 0 136 214 135 601.0 0 135 215 135 601.0 0 135 216 131 601.0 0 131 217 114 601.0 0 114 218 102 601.0 0 102 219 134 601.0 0 134 220 164 601.0 0 164 221 132 601.0 0 132 222 170 601.0 0 170 223 125 601.0 0 125 224 120 601.0 0 120 225 121 601.0 0 121 226 268 601.0 0 268 227 133 601.0 0 133 228 150 601.0 0 150 229 129 601.0 0 129 230 118 601.0 0 118 231 133 601.0 0 133 232 157 601.0 0 157 233 124 601.0 0 124 234 146 601.0 0 146 235 159 601.0 0 159 236 125 601.0 0 125 237 125 601.0 0 125 238 121 601.0 0 121 239 132 601.0 0 132 240 133 601.0 0 133 241 114 601.0 0 114 242 137 601.0 0 137 243 117 601.0 0 117 244 167 601.0 0 167 245 118 601.0 0 118 246 117 601.0 0 117 247 134 601.0 0 134 248 110 601.0 0 110 249 107 601.0 0 107 250 117 601.0 0 117 251 136 601.0 0 136 252 214 601.0 0 214 253 247 601.0 0 247 254 273 601.0 0 273 255 102 601.0 0 102 256 135 601.0 0 135 257 151 601.0 0 151 258 118 601.0 0 118 259 98 601.0 0 98 260 155 601.0 0 155 261 130 601.0 0 130 262 165 601.0 0 165 263 115 601.0 0 115 264 163 601.0 0 163 265 104 601.0 0 104 266 111 601.0 0 111 267 125 601.0 0 125 268 225 601.0 0 225 269 445 601.0 0 445 270 683 601.0 0 683 271 469 601.0 0 469 272 614 601.0 0 614 273 165 601.0 0 165 274 191 601.0 0 191 275 211 601.0 0 211 276 765 601.0 0 765 277 1427 601.0 0 1427 278 2557 601.0 0 2557 279 1336 601.0 0 1336 280 215 601.0 0 215 281 197 601.0 0 197 282 285 601.0 0 285 283 163 601.0 0 163 284 175 601.0 0 175 285 256 601.0 0 256 286 181 601.0 0 181 287 147 601.0 0 147 288 194 601.0 0 194 289 366 601.0 0 366 290 325 601.0 0 325 291 332 601.0 0 332 292 378 601.0 0 378 293 436 601.0 0 436 294 797 601.0 0 797 295 19983 601.0 0 19983 296 9639 601.0 0 9639 297 4121 601.0 0 4121 298 122 601.0 0 122 299 121 601.0 0 121 300 111 601.0 0 111 301 115 601.0 0 115 Finished in 3.80 s (39 us/read; 1.54 M reads/minute). === Summary === Total reads processed: 97,735 Reads with adapters: 3,137 (3.2%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 3,124 (3.2%) Total basepairs processed: 17,626,228 bp Total written (filtered): 854,082 bp (4.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 3137 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 3 0.0 3 0 0 3 19 21 0.0 3 0 20 1 20 3076 0.0 4 2793 276 7 21 5 0.0 4 2 2 1 23 1 0.0 4 0 0 0 1 40 1 0.0 4 0 0 0 0 1 54 1 0.0 4 1 79 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 88 1 0.0 4 1 91 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 1 110 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 1 125 1 0.0 4 0 0 0 0 1 127 13 0.0 4 0 0 0 0 13 128 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 216 1 0.0 4 0 0 0 1 232 1 0.0 4 0 0 0 0 1 289 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.11 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 3,124 Reads with adapters: 108 (3.5%) Reads written (passing filters): 3,124 (100.0%) Total basepairs processed: 854,082 bp Total written (filtered): 852,931 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 108 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.4% C: 3.7% G: 85.2% T: 3.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 5 48.8 0 5 4 3 12.2 0 3 11 95 0.0 1 0 95 12 4 0.0 1 0 4 31 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.