This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCAAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.63 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 46,061 (1.9%) Reads that were too short: 18,371 (0.7%) Reads written (passing filters): 27,690 (1.1%) Total basepairs processed: 740,966,583 bp Total written (filtered): 4,342,520 bp (0.6%) === Adapter 1 === Sequence: CCAAGG; Type: regular 5'; Length: 6; Trimmed: 46061 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 108 601.0 0 108 7 3 601.0 0 3 8 61 601.0 0 61 9 23 601.0 0 23 10 26 601.0 0 26 11 7 601.0 0 7 12 4 601.0 0 4 13 42 601.0 0 42 14 171 601.0 0 171 15 199 601.0 0 199 16 26 601.0 0 26 17 20 601.0 0 20 18 58 601.0 0 58 19 87 601.0 0 87 20 115 601.0 0 115 21 20 601.0 0 20 22 2 601.0 0 2 23 3 601.0 0 3 24 3 601.0 0 3 25 6 601.0 0 6 26 26 601.0 0 26 27 50 601.0 0 50 28 12 601.0 0 12 29 4 601.0 0 4 30 5 601.0 0 5 31 2 601.0 0 2 32 1 601.0 0 1 33 15 601.0 0 15 34 6 601.0 0 6 35 8 601.0 0 8 36 5 601.0 0 5 38 9 601.0 0 9 39 5 601.0 0 5 40 7 601.0 0 7 41 15 601.0 0 15 42 12 601.0 0 12 43 115 601.0 0 115 44 249 601.0 0 249 45 341 601.0 0 341 46 172 601.0 0 172 47 14 601.0 0 14 48 6 601.0 0 6 49 12 601.0 0 12 50 13 601.0 0 13 51 25 601.0 0 25 52 18 601.0 0 18 53 12 601.0 0 12 54 30 601.0 0 30 55 10 601.0 0 10 56 58 601.0 0 58 57 11 601.0 0 11 58 16 601.0 0 16 59 18 601.0 0 18 60 29 601.0 0 29 61 15 601.0 0 15 62 15 601.0 0 15 63 12 601.0 0 12 64 17 601.0 0 17 65 25 601.0 0 25 66 28 601.0 0 28 67 46 601.0 0 46 68 44 601.0 0 44 69 19 601.0 0 19 70 20 601.0 0 20 71 41 601.0 0 41 72 22 601.0 0 22 73 52 601.0 0 52 74 47 601.0 0 47 75 39 601.0 0 39 76 154 601.0 0 154 77 731 601.0 0 731 78 1460 601.0 0 1460 79 2211 601.0 0 2211 80 43 601.0 0 43 81 10 601.0 0 10 82 45 601.0 0 45 83 12 601.0 0 12 84 31 601.0 0 31 85 23 601.0 0 23 86 24 601.0 0 24 87 6 601.0 0 6 88 6 601.0 0 6 89 5 601.0 0 5 90 10 601.0 0 10 91 28 601.0 0 28 92 71 601.0 0 71 93 22 601.0 0 22 94 49 601.0 0 49 95 39 601.0 0 39 96 97 601.0 0 97 97 22 601.0 0 22 98 2 601.0 0 2 99 6 601.0 0 6 100 17 601.0 0 17 101 16 601.0 0 16 102 16 601.0 0 16 103 28 601.0 0 28 104 53 601.0 0 53 105 20 601.0 0 20 106 9 601.0 0 9 107 13 601.0 0 13 108 19 601.0 0 19 109 18 601.0 0 18 110 21 601.0 0 21 111 14 601.0 0 14 112 25 601.0 0 25 113 35 601.0 0 35 114 224 601.0 0 224 115 129 601.0 0 129 116 144 601.0 0 144 117 60 601.0 0 60 118 10 601.0 0 10 119 17 601.0 0 17 120 26 601.0 0 26 121 51 601.0 0 51 122 35 601.0 0 35 123 46 601.0 0 46 124 33 601.0 0 33 125 33 601.0 0 33 126 46 601.0 0 46 127 37 601.0 0 37 128 34 601.0 0 34 129 100 601.0 0 100 130 25 601.0 0 25 131 19 601.0 0 19 132 22 601.0 0 22 133 61 601.0 0 61 134 25 601.0 0 25 135 85 601.0 0 85 136 348 601.0 0 348 137 312 601.0 0 312 138 242 601.0 0 242 139 112 601.0 0 112 140 309 601.0 0 309 141 34 601.0 0 34 142 62 601.0 0 62 143 27 601.0 0 27 144 31 601.0 0 31 145 28 601.0 0 28 146 32 601.0 0 32 147 21 601.0 0 21 148 56 601.0 0 56 149 55 601.0 0 55 150 104 601.0 0 104 151 49 601.0 0 49 152 66 601.0 0 66 153 26 601.0 0 26 154 56 601.0 0 56 155 28 601.0 0 28 156 22 601.0 0 22 157 49 601.0 0 49 158 17 601.0 0 17 159 28 601.0 0 28 160 33 601.0 0 33 161 34 601.0 0 34 162 60 601.0 0 60 163 89 601.0 0 89 164 90 601.0 0 90 165 122 601.0 0 122 166 120 601.0 0 120 167 33 601.0 0 33 168 21 601.0 0 21 169 38 601.0 0 38 170 38 601.0 0 38 171 45 601.0 0 45 172 69 601.0 0 69 173 1245 601.0 0 1245 174 641 601.0 0 641 175 703 601.0 0 703 176 63 601.0 0 63 177 56 601.0 0 56 178 55 601.0 0 55 179 135 601.0 0 135 180 801 601.0 0 801 181 770 601.0 0 770 182 1415 601.0 0 1415 183 88 601.0 0 88 184 69 601.0 0 69 185 77 601.0 0 77 186 60 601.0 0 60 187 60 601.0 0 60 188 114 601.0 0 114 189 219 601.0 0 219 190 394 601.0 0 394 191 1904 601.0 0 1904 192 2894 601.0 0 2894 193 3028 601.0 0 3028 194 373 601.0 0 373 195 18 601.0 0 18 196 18 601.0 0 18 197 25 601.0 0 25 198 29 601.0 0 29 199 30 601.0 0 30 200 27 601.0 0 27 201 43 601.0 0 43 202 256 601.0 0 256 203 381 601.0 0 381 204 576 601.0 0 576 205 552 601.0 0 552 206 71 601.0 0 71 207 20 601.0 0 20 208 204 601.0 0 204 209 245 601.0 0 245 210 186 601.0 0 186 211 63 601.0 0 63 212 18 601.0 0 18 213 19 601.0 0 19 214 21 601.0 0 21 215 24 601.0 0 24 216 59 601.0 0 59 217 97 601.0 0 97 218 89 601.0 0 89 219 44 601.0 0 44 220 14 601.0 0 14 221 19 601.0 0 19 222 22 601.0 0 22 223 26 601.0 0 26 224 53 601.0 0 53 225 63 601.0 0 63 226 37 601.0 0 37 227 78 601.0 0 78 228 30 601.0 0 30 229 42 601.0 0 42 230 12 601.0 0 12 231 52 601.0 0 52 232 66 601.0 0 66 233 98 601.0 0 98 234 68 601.0 0 68 235 74 601.0 0 74 236 78 601.0 0 78 237 34 601.0 0 34 238 33 601.0 0 33 239 26 601.0 0 26 240 37 601.0 0 37 241 24 601.0 0 24 242 32 601.0 0 32 243 17 601.0 0 17 244 34 601.0 0 34 245 37 601.0 0 37 246 43 601.0 0 43 247 35 601.0 0 35 248 40 601.0 0 40 249 29 601.0 0 29 250 27 601.0 0 27 251 65 601.0 0 65 252 397 601.0 0 397 253 485 601.0 0 485 254 377 601.0 0 377 255 61 601.0 0 61 256 25 601.0 0 25 257 29 601.0 0 29 258 269 601.0 0 269 259 412 601.0 0 412 260 257 601.0 0 257 261 483 601.0 0 483 262 492 601.0 0 492 263 171 601.0 0 171 264 207 601.0 0 207 265 280 601.0 0 280 266 175 601.0 0 175 267 73 601.0 0 73 268 31 601.0 0 31 269 40 601.0 0 40 270 91 601.0 0 91 271 106 601.0 0 106 272 135 601.0 0 135 273 105 601.0 0 105 274 77 601.0 0 77 275 90 601.0 0 90 276 60 601.0 0 60 277 69 601.0 0 69 278 69 601.0 0 69 279 118 601.0 0 118 280 150 601.0 0 150 281 103 601.0 0 103 282 86 601.0 0 86 283 399 601.0 0 399 284 3193 601.0 0 3193 285 1602 601.0 0 1602 286 1846 601.0 0 1846 287 142 601.0 0 142 288 187 601.0 0 187 289 66 601.0 0 66 290 73 601.0 0 73 291 106 601.0 0 106 292 65 601.0 0 65 293 72 601.0 0 72 294 58 601.0 0 58 295 156 601.0 0 156 296 187 601.0 0 187 297 168 601.0 0 168 298 78 601.0 0 78 299 43 601.0 0 43 300 27 601.0 0 27 301 110 601.0 0 110 Finished in 0.92 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 27,690 Reads with adapters: 567 (2.0%) Reads that were too short: 128 (0.5%) Reads written (passing filters): 439 (1.6%) Total basepairs processed: 4,342,520 bp Total written (filtered): 78,334 bp (1.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 567 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 0 3 16 2 0.0 3 0 0 1 1 17 14 0.0 3 7 5 2 18 139 0.0 3 0 0 1 138 19 9 0.0 3 0 5 0 4 20 96 0.0 3 88 5 2 1 21 24 0.0 3 0 0 1 23 22 2 0.0 3 0 0 0 2 23 2 0.0 3 0 0 0 2 25 3 0.0 3 0 0 0 3 26 2 0.0 3 0 0 1 1 29 1 0.0 3 0 0 0 1 30 19 0.0 3 1 0 0 18 31 1 0.0 3 0 0 0 1 33 4 0.0 3 0 0 1 3 34 3 0.0 3 0 0 0 3 35 1 0.0 3 0 0 0 1 36 2 0.0 3 0 0 0 2 39 3 0.0 3 0 0 0 3 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 0 1 42 3 0.0 3 0 0 0 3 43 3 0.0 3 0 0 0 3 44 4 0.0 3 0 0 0 4 45 3 0.0 3 0 0 0 3 48 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 53 9 0.0 3 0 0 0 9 54 1 0.0 3 0 0 0 1 55 3 0.0 3 0 0 0 3 56 8 0.0 3 0 0 0 8 57 1 0.0 3 0 0 0 1 58 3 0.0 3 0 0 0 3 59 3 0.0 3 0 0 0 3 61 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 69 4 0.0 3 0 0 0 4 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 74 2 0.0 3 0 0 0 2 77 4 0.0 3 0 0 0 4 78 3 0.0 3 0 0 0 3 80 2 0.0 3 0 0 0 2 81 16 0.0 3 7 4 3 2 82 1 0.0 3 0 0 0 1 83 6 0.0 3 0 0 0 6 84 1 0.0 3 0 0 0 1 85 2 0.0 3 0 0 0 2 87 2 0.0 3 0 0 0 2 90 3 0.0 3 0 0 0 3 95 4 0.0 3 0 0 0 4 96 4 0.0 3 0 0 0 4 97 2 0.0 3 0 0 0 2 98 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 103 2 0.0 3 0 0 0 2 104 2 0.0 3 0 0 0 2 106 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 0 1 108 3 0.0 3 0 0 0 3 109 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 0 2 118 2 0.0 3 0 0 0 2 119 14 0.0 3 0 0 0 14 120 2 0.0 3 0 0 1 1 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 129 2 0.0 3 0 0 0 2 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 143 1 0.0 3 0 0 0 1 145 4 0.0 3 0 0 0 4 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 5 0.0 3 0 0 0 5 149 3 0.0 3 0 0 0 3 151 3 0.0 3 0 0 0 3 153 1 0.0 3 0 0 0 1 154 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 158 7 0.0 3 0 0 1 6 160 1 0.0 3 0 0 0 1 162 2 0.0 3 0 0 0 2 163 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 173 3 0.0 3 0 0 0 3 175 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 1 186 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 201 2 0.0 3 0 0 0 2 203 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 208 2 0.0 3 0 0 0 2 213 1 0.0 3 0 0 1 235 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 245 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (23 us/read; 2.63 M reads/minute). === Summary === Total reads processed: 439 Reads with adapters: 7 (1.6%) Reads written (passing filters): 439 (100.0%) Total basepairs processed: 78,334 bp Total written (filtered): 78,310 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 7 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 42.9% C: 14.3% G: 28.6% T: 14.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 4 6.9 0 4 4 3 1.7 0 3