This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.98 s (27 us/read; 2.24 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 282,386 (11.5%) Reads that were too short: 12,579 (0.5%) Reads written (passing filters): 269,807 (11.0%) Total basepairs processed: 740,966,583 bp Total written (filtered): 78,214,728 bp (10.6%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 282386 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2356 601.0 0 2356 7 148829 601.0 0 148829 8 108006 601.0 0 108006 9 96 601.0 0 96 10 19 601.0 0 19 11 11 601.0 0 11 12 14 601.0 0 14 13 15 601.0 0 15 14 18 601.0 0 18 15 16 601.0 0 16 16 17 601.0 0 17 17 18 601.0 0 18 18 31 601.0 0 31 19 40 601.0 0 40 20 65 601.0 0 65 21 17 601.0 0 17 22 21 601.0 0 21 23 26 601.0 0 26 24 8 601.0 0 8 25 16 601.0 0 16 26 31 601.0 0 31 27 21 601.0 0 21 28 28 601.0 0 28 29 18 601.0 0 18 30 23 601.0 0 23 31 25 601.0 0 25 32 19 601.0 0 19 33 33 601.0 0 33 34 38 601.0 0 38 35 62 601.0 0 62 36 243 601.0 0 243 37 467 601.0 0 467 38 146 601.0 0 146 39 35 601.0 0 35 40 24 601.0 0 24 41 27 601.0 0 27 42 28 601.0 0 28 43 385 601.0 0 385 44 903 601.0 0 903 45 572 601.0 0 572 46 21 601.0 0 21 47 8 601.0 0 8 48 17 601.0 0 17 49 19 601.0 0 19 50 18 601.0 0 18 51 19 601.0 0 19 52 24 601.0 0 24 53 21 601.0 0 21 54 23 601.0 0 23 55 34 601.0 0 34 56 39 601.0 0 39 57 39 601.0 0 39 58 26 601.0 0 26 59 18 601.0 0 18 60 15 601.0 0 15 61 33 601.0 0 33 62 20 601.0 0 20 63 22 601.0 0 22 64 21 601.0 0 21 65 16 601.0 0 16 66 37 601.0 0 37 67 17 601.0 0 17 68 25 601.0 0 25 69 20 601.0 0 20 70 41 601.0 0 41 71 136 601.0 0 136 72 26 601.0 0 26 73 46 601.0 0 46 74 26 601.0 0 26 75 20 601.0 0 20 76 17 601.0 0 17 77 18 601.0 0 18 78 38 601.0 0 38 79 33 601.0 0 33 80 23 601.0 0 23 81 22 601.0 0 22 82 16 601.0 0 16 83 22 601.0 0 22 84 28 601.0 0 28 85 30 601.0 0 30 86 20 601.0 0 20 87 24 601.0 0 24 88 25 601.0 0 25 89 26 601.0 0 26 90 27 601.0 0 27 91 26 601.0 0 26 92 30 601.0 0 30 93 23 601.0 0 23 94 30 601.0 0 30 95 27 601.0 0 27 96 59 601.0 0 59 97 15 601.0 0 15 98 13 601.0 0 13 99 22 601.0 0 22 100 15 601.0 0 15 101 15 601.0 0 15 102 12 601.0 0 12 103 23 601.0 0 23 104 12 601.0 0 12 105 31 601.0 0 31 106 23 601.0 0 23 107 25 601.0 0 25 108 40 601.0 0 40 109 27 601.0 0 27 110 16 601.0 0 16 111 21 601.0 0 21 112 37 601.0 0 37 113 30 601.0 0 30 114 27 601.0 0 27 115 37 601.0 0 37 116 19 601.0 0 19 117 13 601.0 0 13 118 26 601.0 0 26 119 23 601.0 0 23 120 32 601.0 0 32 121 32 601.0 0 32 122 70 601.0 0 70 123 34 601.0 0 34 124 19 601.0 0 19 125 50 601.0 0 50 126 121 601.0 0 121 127 68 601.0 0 68 128 143 601.0 0 143 129 67 601.0 0 67 130 59 601.0 0 59 131 93 601.0 0 93 132 92 601.0 0 92 133 47 601.0 0 47 134 47 601.0 0 47 135 306 601.0 0 306 136 225 601.0 0 225 137 300 601.0 0 300 138 38 601.0 0 38 139 76 601.0 0 76 140 102 601.0 0 102 141 167 601.0 0 167 142 98 601.0 0 98 143 88 601.0 0 88 144 41 601.0 0 41 145 44 601.0 0 44 146 42 601.0 0 42 147 61 601.0 0 61 148 173 601.0 0 173 149 102 601.0 0 102 150 95 601.0 0 95 151 37 601.0 0 37 152 47 601.0 0 47 153 64 601.0 0 64 154 35 601.0 0 35 155 53 601.0 0 53 156 47 601.0 0 47 157 33 601.0 0 33 158 42 601.0 0 42 159 51 601.0 0 51 160 25 601.0 0 25 161 45 601.0 0 45 162 26 601.0 0 26 163 30 601.0 0 30 164 33 601.0 0 33 165 34 601.0 0 34 166 39 601.0 0 39 167 60 601.0 0 60 168 46 601.0 0 46 169 84 601.0 0 84 170 35 601.0 0 35 171 33 601.0 0 33 172 33 601.0 0 33 173 36 601.0 0 36 174 25 601.0 0 25 175 37 601.0 0 37 176 33 601.0 0 33 177 39 601.0 0 39 178 28 601.0 0 28 179 32 601.0 0 32 180 27 601.0 0 27 181 51 601.0 0 51 182 81 601.0 0 81 183 44 601.0 0 44 184 42 601.0 0 42 185 55 601.0 0 55 186 50 601.0 0 50 187 53 601.0 0 53 188 35 601.0 0 35 189 38 601.0 0 38 190 43 601.0 0 43 191 84 601.0 0 84 192 31 601.0 0 31 193 43 601.0 0 43 194 50 601.0 0 50 195 62 601.0 0 62 196 43 601.0 0 43 197 34 601.0 0 34 198 27 601.0 0 27 199 33 601.0 0 33 200 42 601.0 0 42 201 45 601.0 0 45 202 60 601.0 0 60 203 50 601.0 0 50 204 27 601.0 0 27 205 30 601.0 0 30 206 52 601.0 0 52 207 115 601.0 0 115 208 170 601.0 0 170 209 151 601.0 0 151 210 124 601.0 0 124 211 63 601.0 0 63 212 193 601.0 0 193 213 81 601.0 0 81 214 159 601.0 0 159 215 143 601.0 0 143 216 261 601.0 0 261 217 51 601.0 0 51 218 28 601.0 0 28 219 57 601.0 0 57 220 51 601.0 0 51 221 86 601.0 0 86 222 64 601.0 0 64 223 51 601.0 0 51 224 51 601.0 0 51 225 67 601.0 0 67 226 71 601.0 0 71 227 50 601.0 0 50 228 87 601.0 0 87 229 81 601.0 0 81 230 132 601.0 0 132 231 53 601.0 0 53 232 70 601.0 0 70 233 149 601.0 0 149 234 153 601.0 0 153 235 155 601.0 0 155 236 91 601.0 0 91 237 107 601.0 0 107 238 40 601.0 0 40 239 41 601.0 0 41 240 51 601.0 0 51 241 47 601.0 0 47 242 62 601.0 0 62 243 59 601.0 0 59 244 54 601.0 0 54 245 56 601.0 0 56 246 49 601.0 0 49 247 30 601.0 0 30 248 29 601.0 0 29 249 30 601.0 0 30 250 39 601.0 0 39 251 49 601.0 0 49 252 64 601.0 0 64 253 53 601.0 0 53 254 68 601.0 0 68 255 66 601.0 0 66 256 72 601.0 0 72 257 57 601.0 0 57 258 67 601.0 0 67 259 93 601.0 0 93 260 97 601.0 0 97 261 78 601.0 0 78 262 73 601.0 0 73 263 53 601.0 0 53 264 65 601.0 0 65 265 64 601.0 0 64 266 51 601.0 0 51 267 69 601.0 0 69 268 143 601.0 0 143 269 387 601.0 0 387 270 315 601.0 0 315 271 353 601.0 0 353 272 114 601.0 0 114 273 111 601.0 0 111 274 120 601.0 0 120 275 172 601.0 0 172 276 120 601.0 0 120 277 79 601.0 0 79 278 63 601.0 0 63 279 135 601.0 0 135 280 149 601.0 0 149 281 264 601.0 0 264 282 133 601.0 0 133 283 104 601.0 0 104 284 79 601.0 0 79 285 105 601.0 0 105 286 90 601.0 0 90 287 65 601.0 0 65 288 111 601.0 0 111 289 96 601.0 0 96 290 152 601.0 0 152 291 100 601.0 0 100 292 102 601.0 0 102 293 197 601.0 0 197 294 797 601.0 0 797 295 1021 601.0 0 1021 296 606 601.0 0 606 297 311 601.0 0 311 298 154 601.0 0 154 299 97 601.0 0 97 300 167 601.0 0 167 301 457 601.0 0 457 Finished in 12.00 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 269,807 Reads with adapters: 146,759 (54.4%) Reads that were too short: 223 (0.1%) Reads written (passing filters): 146,536 (54.3%) Total basepairs processed: 78,214,728 bp Total written (filtered): 40,563,028 bp (51.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 146759 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 33 0.0 2 0 0 0 33 15 132 0.0 3 0 0 82 50 16 1441 0.0 3 0 1113 197 131 17 143252 0.0 3 130971 9889 1914 478 18 371 0.0 3 33 238 32 68 19 555 0.0 3 0 0 316 239 20 564 0.0 3 0 0 0 564 23 2 0.0 3 0 1 0 1 24 1 0.0 3 1 25 1 0.0 3 0 0 0 1 27 2 0.0 3 0 0 0 2 28 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 32 3 0.0 3 0 0 0 3 36 2 0.0 3 1 0 0 1 38 1 0.0 3 1 40 1 0.0 3 0 0 1 42 1 0.0 3 0 0 0 1 43 15 0.0 3 0 0 0 15 45 2 0.0 3 0 0 0 2 46 4 0.0 3 0 0 0 4 48 2 0.0 3 0 0 0 2 51 3 0.0 3 0 0 0 3 52 1 0.0 3 0 0 0 1 53 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 61 2 0.0 3 0 0 0 2 65 5 0.0 3 0 0 0 5 74 1 0.0 3 0 0 0 1 79 4 0.0 3 0 0 0 4 81 1 0.0 3 0 0 0 1 86 4 0.0 3 0 0 1 3 88 1 0.0 3 0 0 0 1 94 2 0.0 3 0 0 0 2 95 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 123 4 0.0 3 0 0 0 4 124 1 0.0 3 0 0 0 1 125 3 0.0 3 0 0 0 3 126 1 0.0 3 0 0 0 1 128 13 0.0 3 0 0 0 13 129 8 0.0 3 0 0 0 8 131 2 0.0 3 0 0 0 2 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 135 8 0.0 3 0 0 0 8 136 2 0.0 3 0 0 0 2 138 3 0.0 3 0 0 0 3 139 1 0.0 3 0 0 1 145 2 0.0 3 0 0 0 2 146 3 0.0 3 0 0 0 3 147 3 0.0 3 0 0 0 3 148 7 0.0 3 0 0 0 7 149 2 0.0 3 0 0 0 2 151 3 0.0 3 0 0 0 3 153 2 0.0 3 0 0 0 2 154 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 156 8 0.0 3 0 0 0 8 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 2 0.0 3 0 0 0 2 163 5 0.0 3 0 0 0 5 164 3 0.0 3 0 0 0 3 165 3 0.0 3 0 0 0 3 167 2 0.0 3 0 0 0 2 168 3 0.0 3 0 0 0 3 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 1 174 2 0.0 3 0 0 0 2 175 1 0.0 3 0 0 1 178 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 1 1 183 3 0.0 3 0 0 0 3 184 4 0.0 3 0 0 0 4 185 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 187 2 0.0 3 0 0 0 2 188 3 0.0 3 0 0 0 3 190 1 0.0 3 0 0 1 191 2 0.0 3 0 0 0 2 192 9 0.0 3 0 0 1 8 193 1 0.0 3 0 0 0 1 194 16 0.0 3 0 0 0 16 199 2 0.0 3 0 0 0 2 204 2 0.0 3 0 0 0 2 205 9 0.0 3 0 0 0 9 206 4 0.0 3 0 0 0 4 207 9 0.0 3 0 0 0 9 208 2 0.0 3 0 0 0 2 209 2 0.0 3 0 0 0 2 216 2 0.0 3 0 0 0 2 217 1 0.0 3 0 0 0 1 218 10 0.0 3 0 0 0 10 219 1 0.0 3 0 0 0 1 221 3 0.0 3 0 0 0 3 222 3 0.0 3 0 0 0 3 223 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 232 1 0.0 3 0 0 0 1 238 2 0.0 3 0 0 0 2 239 4 0.0 3 0 0 0 4 241 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 244 2 0.0 3 0 0 0 2 247 1 0.0 3 0 0 0 1 248 1 0.0 3 0 0 0 1 249 3 0.0 3 0 0 0 3 250 1 0.0 3 0 0 0 1 251 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 262 2 0.0 3 0 0 0 2 268 1 0.0 3 0 0 0 1 270 2 0.0 3 0 0 0 2 271 2 0.0 3 0 0 0 2 272 1 0.0 3 0 0 0 1 278 2 0.0 3 0 0 0 2 279 10 0.0 3 0 0 0 10 280 13 0.0 3 0 0 0 13 281 26 0.0 3 0 0 0 26 282 26 0.0 3 0 0 1 25 283 10 0.0 3 0 0 0 10 284 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 288 2 0.0 3 0 0 0 2 289 1 0.0 3 0 0 0 1 290 1 0.0 3 0 0 0 1 291 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.06 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 146,536 Reads with adapters: 609 (0.4%) Reads written (passing filters): 146,536 (100.0%) Total basepairs processed: 40,563,028 bp Total written (filtered): 40,561,035 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 609 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 17.7% C: 51.6% G: 17.2% T: 13.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 503 2289.6 0 503 4 60 572.4 0 60 5 34 143.1 0 34 6 11 35.8 0 11 8 1 2.2 0 1