This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACATTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.98 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 284,998 (11.6%) Reads that were too short: 5,477 (0.2%) Reads written (passing filters): 279,521 (11.4%) Total basepairs processed: 740,966,583 bp Total written (filtered): 81,382,994 bp (11.0%) === Adapter 1 === Sequence: ACATTG; Type: regular 5'; Length: 6; Trimmed: 284998 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1847 601.0 0 1847 7 119601 601.0 0 119601 8 151508 601.0 0 151508 9 130 601.0 0 130 10 22 601.0 0 22 11 12 601.0 0 12 12 14 601.0 0 14 13 8 601.0 0 8 14 8 601.0 0 8 15 25 601.0 0 25 16 23 601.0 0 23 17 20 601.0 0 20 18 29 601.0 0 29 19 22 601.0 0 22 20 34 601.0 0 34 21 18 601.0 0 18 22 11 601.0 0 11 23 23 601.0 0 23 24 16 601.0 0 16 25 22 601.0 0 22 26 14 601.0 0 14 27 18 601.0 0 18 28 17 601.0 0 17 29 20 601.0 0 20 30 21 601.0 0 21 31 19 601.0 0 19 32 24 601.0 0 24 33 26 601.0 0 26 34 29 601.0 0 29 35 21 601.0 0 21 36 127 601.0 0 127 37 118 601.0 0 118 38 89 601.0 0 89 39 19 601.0 0 19 40 12 601.0 0 12 41 25 601.0 0 25 42 23 601.0 0 23 43 79 601.0 0 79 44 104 601.0 0 104 45 101 601.0 0 101 46 12 601.0 0 12 47 24 601.0 0 24 48 19 601.0 0 19 49 41 601.0 0 41 50 20 601.0 0 20 51 29 601.0 0 29 52 30 601.0 0 30 53 33 601.0 0 33 54 32 601.0 0 32 55 29 601.0 0 29 56 28 601.0 0 28 57 40 601.0 0 40 58 44 601.0 0 44 59 40 601.0 0 40 60 22 601.0 0 22 61 29 601.0 0 29 62 26 601.0 0 26 63 20 601.0 0 20 64 19 601.0 0 19 65 20 601.0 0 20 66 30 601.0 0 30 67 26 601.0 0 26 68 17 601.0 0 17 69 31 601.0 0 31 70 27 601.0 0 27 71 165 601.0 0 165 72 34 601.0 0 34 73 28 601.0 0 28 74 20 601.0 0 20 75 31 601.0 0 31 76 28 601.0 0 28 77 25 601.0 0 25 78 26 601.0 0 26 79 26 601.0 0 26 80 14 601.0 0 14 81 28 601.0 0 28 82 21 601.0 0 21 83 28 601.0 0 28 84 29 601.0 0 29 85 23 601.0 0 23 86 13 601.0 0 13 87 30 601.0 0 30 88 24 601.0 0 24 89 20 601.0 0 20 90 26 601.0 0 26 91 26 601.0 0 26 92 17 601.0 0 17 93 32 601.0 0 32 94 35 601.0 0 35 95 43 601.0 0 43 96 42 601.0 0 42 97 13 601.0 0 13 98 11 601.0 0 11 99 26 601.0 0 26 100 19 601.0 0 19 101 25 601.0 0 25 102 24 601.0 0 24 103 30 601.0 0 30 104 21 601.0 0 21 105 23 601.0 0 23 106 25 601.0 0 25 107 14 601.0 0 14 108 30 601.0 0 30 109 15 601.0 0 15 110 18 601.0 0 18 111 26 601.0 0 26 112 26 601.0 0 26 113 23 601.0 0 23 114 15 601.0 0 15 115 44 601.0 0 44 116 25 601.0 0 25 117 11 601.0 0 11 118 28 601.0 0 28 119 17 601.0 0 17 120 25 601.0 0 25 121 17 601.0 0 17 122 50 601.0 0 50 123 21 601.0 0 21 124 7 601.0 0 7 125 23 601.0 0 23 126 76 601.0 0 76 127 24 601.0 0 24 128 61 601.0 0 61 129 48 601.0 0 48 130 38 601.0 0 38 131 74 601.0 0 74 132 95 601.0 0 95 133 49 601.0 0 49 134 50 601.0 0 50 135 161 601.0 0 161 136 124 601.0 0 124 137 156 601.0 0 156 138 25 601.0 0 25 139 101 601.0 0 101 140 74 601.0 0 74 141 134 601.0 0 134 142 48 601.0 0 48 143 61 601.0 0 61 144 27 601.0 0 27 145 34 601.0 0 34 146 26 601.0 0 26 147 43 601.0 0 43 148 88 601.0 0 88 149 65 601.0 0 65 150 49 601.0 0 49 151 21 601.0 0 21 152 23 601.0 0 23 153 65 601.0 0 65 154 25 601.0 0 25 155 29 601.0 0 29 156 23 601.0 0 23 157 10 601.0 0 10 158 37 601.0 0 37 159 24 601.0 0 24 160 25 601.0 0 25 161 23 601.0 0 23 162 21 601.0 0 21 163 19 601.0 0 19 164 20 601.0 0 20 165 27 601.0 0 27 166 21 601.0 0 21 167 27 601.0 0 27 168 22 601.0 0 22 169 26 601.0 0 26 170 18 601.0 0 18 171 16 601.0 0 16 172 24 601.0 0 24 173 24 601.0 0 24 174 18 601.0 0 18 175 25 601.0 0 25 176 20 601.0 0 20 177 17 601.0 0 17 178 26 601.0 0 26 179 28 601.0 0 28 180 25 601.0 0 25 181 21 601.0 0 21 182 42 601.0 0 42 183 14 601.0 0 14 184 25 601.0 0 25 185 32 601.0 0 32 186 24 601.0 0 24 187 24 601.0 0 24 188 26 601.0 0 26 189 18 601.0 0 18 190 20 601.0 0 20 191 86 601.0 0 86 192 26 601.0 0 26 193 26 601.0 0 26 194 26 601.0 0 26 195 31 601.0 0 31 196 45 601.0 0 45 197 25 601.0 0 25 198 22 601.0 0 22 199 13 601.0 0 13 200 20 601.0 0 20 201 22 601.0 0 22 202 30 601.0 0 30 203 21 601.0 0 21 204 16 601.0 0 16 205 12 601.0 0 12 206 77 601.0 0 77 207 54 601.0 0 54 208 77 601.0 0 77 209 55 601.0 0 55 210 68 601.0 0 68 211 31 601.0 0 31 212 148 601.0 0 148 213 30 601.0 0 30 214 52 601.0 0 52 215 66 601.0 0 66 216 105 601.0 0 105 217 24 601.0 0 24 218 10 601.0 0 10 219 25 601.0 0 25 220 27 601.0 0 27 221 46 601.0 0 46 222 27 601.0 0 27 223 31 601.0 0 31 224 29 601.0 0 29 225 52 601.0 0 52 226 26 601.0 0 26 227 23 601.0 0 23 228 45 601.0 0 45 229 27 601.0 0 27 230 63 601.0 0 63 231 19 601.0 0 19 232 34 601.0 0 34 233 37 601.0 0 37 234 42 601.0 0 42 235 51 601.0 0 51 236 27 601.0 0 27 237 32 601.0 0 32 238 22 601.0 0 22 239 18 601.0 0 18 240 39 601.0 0 39 241 22 601.0 0 22 242 35 601.0 0 35 243 26 601.0 0 26 244 37 601.0 0 37 245 37 601.0 0 37 246 32 601.0 0 32 247 9 601.0 0 9 248 21 601.0 0 21 249 23 601.0 0 23 250 20 601.0 0 20 251 20 601.0 0 20 252 22 601.0 0 22 253 36 601.0 0 36 254 45 601.0 0 45 255 30 601.0 0 30 256 38 601.0 0 38 257 31 601.0 0 31 258 26 601.0 0 26 259 29 601.0 0 29 260 42 601.0 0 42 261 31 601.0 0 31 262 29 601.0 0 29 263 27 601.0 0 27 264 39 601.0 0 39 265 46 601.0 0 46 266 36 601.0 0 36 267 51 601.0 0 51 268 41 601.0 0 41 269 137 601.0 0 137 270 112 601.0 0 112 271 119 601.0 0 119 272 58 601.0 0 58 273 44 601.0 0 44 274 58 601.0 0 58 275 79 601.0 0 79 276 53 601.0 0 53 277 41 601.0 0 41 278 34 601.0 0 34 279 91 601.0 0 91 280 89 601.0 0 89 281 206 601.0 0 206 282 51 601.0 0 51 283 44 601.0 0 44 284 36 601.0 0 36 285 73 601.0 0 73 286 27 601.0 0 27 287 27 601.0 0 27 288 56 601.0 0 56 289 42 601.0 0 42 290 66 601.0 0 66 291 39 601.0 0 39 292 48 601.0 0 48 293 71 601.0 0 71 294 256 601.0 0 256 295 364 601.0 0 364 296 203 601.0 0 203 297 133 601.0 0 133 298 49 601.0 0 49 299 52 601.0 0 52 300 54 601.0 0 54 301 166 601.0 0 166 Finished in 13.29 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 279,521 Reads with adapters: 156,375 (55.9%) Reads that were too short: 36 (0.0%) Reads written (passing filters): 156,339 (55.9%) Total basepairs processed: 81,382,994 bp Total written (filtered): 42,604,959 bp (52.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 156375 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 0 0 0 3 1 18 50 0.0 3 0 0 49 1 19 1638 0.0 3 0 1591 40 5 2 20 153327 0.0 4 150797 2426 86 16 2 21 351 0.0 4 82 262 6 1 22 20 0.0 4 3 2 14 1 23 170 0.0 4 74 2 1 76 17 24 7 0.0 4 1 2 0 1 3 25 2 0.0 4 1 0 1 26 1 0.0 4 0 0 0 1 27 2 0.0 4 1 0 0 1 29 1 0.0 4 1 31 1 0.0 4 1 35 2 0.0 4 1 1 42 1 0.0 4 1 68 1 0.0 4 1 86 1 0.0 4 1 91 1 0.0 4 0 0 0 0 1 92 2 0.0 4 0 0 0 0 2 98 3 0.0 4 0 0 0 0 3 125 2 0.0 4 0 0 0 0 2 126 7 0.0 4 0 0 0 0 7 127 734 0.0 4 0 0 0 0 734 128 2 0.0 4 0 0 0 0 2 138 1 0.0 4 1 139 1 0.0 4 1 146 1 0.0 4 0 0 0 1 150 1 0.0 4 1 153 7 0.0 4 0 0 0 1 6 185 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 1 200 1 0.0 4 1 205 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 226 1 0.0 4 0 0 0 0 1 228 4 0.0 4 0 0 0 0 4 229 3 0.0 4 0 0 0 0 3 233 2 0.0 4 0 0 0 0 2 234 3 0.0 4 0 0 0 0 3 235 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 257 2 0.0 4 0 0 0 0 2 258 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 1 260 1 0.0 4 0 0 0 0 1 262 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 294 4 0.0 4 0 0 0 0 4 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.48 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 156,339 Reads with adapters: 14,888 (9.5%) Reads written (passing filters): 156,339 (100.0%) Total basepairs processed: 42,604,959 bp Total written (filtered): 42,437,049 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 14888 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.4% C: 4.0% G: 92.7% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 85 2442.8 0 85 4 42 610.7 0 42 5 18 152.7 0 18 6 3 38.2 0 3 7 3 9.5 0 3 8 1 2.4 0 1 9 238 0.6 0 4 234 10 4663 0.1 1 188 4475 11 9738 0.0 1 65 9673 12 26 0.0 1 1 25 13 8 0.0 1 0 8 14 2 0.0 1 0 2 15 2 0.0 1 0 2 44 1 0.0 1 1 53 1 0.0 1 0 1 72 1 0.0 1 0 1 90 1 0.0 1 1 105 1 0.0 1 1 109 1 0.0 1 1 123 1 0.0 1 1 148 4 0.0 1 4 151 15 0.0 1 0 15 170 2 0.0 1 2 178 1 0.0 1 0 1 190 5 0.0 1 0 5 192 1 0.0 1 0 1 209 6 0.0 1 4 2 229 1 0.0 1 0 1 230 1 0.0 1 1 260 16 0.0 1 16 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.