This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGCGAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.66 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 73,095 (3.0%) Reads that were too short: 11,513 (0.5%) Reads written (passing filters): 61,582 (2.5%) Total basepairs processed: 740,966,583 bp Total written (filtered): 16,556,396 bp (2.2%) === Adapter 1 === Sequence: CGCGAT; Type: regular 5'; Length: 6; Trimmed: 73095 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1184 601.0 0 1184 7 25503 601.0 0 25503 8 286 601.0 0 286 9 20743 601.0 0 20743 10 50 601.0 0 50 11 39 601.0 0 39 12 27 601.0 0 27 13 37 601.0 0 37 14 35 601.0 0 35 15 36 601.0 0 36 16 38 601.0 0 38 17 38 601.0 0 38 18 35 601.0 0 35 19 29 601.0 0 29 20 30 601.0 0 30 21 50 601.0 0 50 22 31 601.0 0 31 23 47 601.0 0 47 24 46 601.0 0 46 25 31 601.0 0 31 26 51 601.0 0 51 27 36 601.0 0 36 28 41 601.0 0 41 29 37 601.0 0 37 30 39 601.0 0 39 31 27 601.0 0 27 32 38 601.0 0 38 33 30 601.0 0 30 34 39 601.0 0 39 35 36 601.0 0 36 36 22 601.0 0 22 37 42 601.0 0 42 38 59 601.0 0 59 39 46 601.0 0 46 40 30 601.0 0 30 41 29 601.0 0 29 42 107 601.0 0 107 43 66 601.0 0 66 44 77 601.0 0 77 45 38 601.0 0 38 46 23 601.0 0 23 47 27 601.0 0 27 48 47 601.0 0 47 49 36 601.0 0 36 50 42 601.0 0 42 51 26 601.0 0 26 52 32 601.0 0 32 53 28 601.0 0 28 54 33 601.0 0 33 55 41 601.0 0 41 56 30 601.0 0 30 57 20 601.0 0 20 58 57 601.0 0 57 59 28 601.0 0 28 60 30 601.0 0 30 61 30 601.0 0 30 62 30 601.0 0 30 63 45 601.0 0 45 64 31 601.0 0 31 65 38 601.0 0 38 66 60 601.0 0 60 67 60 601.0 0 60 68 31 601.0 0 31 69 39 601.0 0 39 70 36 601.0 0 36 71 36 601.0 0 36 72 42 601.0 0 42 73 44 601.0 0 44 74 52 601.0 0 52 75 66 601.0 0 66 76 54 601.0 0 54 77 40 601.0 0 40 78 30 601.0 0 30 79 98 601.0 0 98 80 65 601.0 0 65 81 75 601.0 0 75 82 26 601.0 0 26 83 39 601.0 0 39 84 30 601.0 0 30 85 44 601.0 0 44 86 31 601.0 0 31 87 61 601.0 0 61 88 55 601.0 0 55 89 57 601.0 0 57 90 68 601.0 0 68 91 49 601.0 0 49 92 150 601.0 0 150 93 578 601.0 0 578 94 1534 601.0 0 1534 95 49 601.0 0 49 96 56 601.0 0 56 97 39 601.0 0 39 98 46 601.0 0 46 99 38 601.0 0 38 100 49 601.0 0 49 101 36 601.0 0 36 102 30 601.0 0 30 103 31 601.0 0 31 104 53 601.0 0 53 105 58 601.0 0 58 106 43 601.0 0 43 107 34 601.0 0 34 108 54 601.0 0 54 109 60 601.0 0 60 110 63 601.0 0 63 111 58 601.0 0 58 112 188 601.0 0 188 113 48 601.0 0 48 114 57 601.0 0 57 115 62 601.0 0 62 116 36 601.0 0 36 117 65 601.0 0 65 118 44 601.0 0 44 119 50 601.0 0 50 120 48 601.0 0 48 121 59 601.0 0 59 122 49 601.0 0 49 123 310 601.0 0 310 124 431 601.0 0 431 125 673 601.0 0 673 126 46 601.0 0 46 127 50 601.0 0 50 128 73 601.0 0 73 129 368 601.0 0 368 130 118 601.0 0 118 131 79 601.0 0 79 132 80 601.0 0 80 133 41 601.0 0 41 134 26 601.0 0 26 135 37 601.0 0 37 136 44 601.0 0 44 137 38 601.0 0 38 138 46 601.0 0 46 139 49 601.0 0 49 140 50 601.0 0 50 141 42 601.0 0 42 142 53 601.0 0 53 143 41 601.0 0 41 144 41 601.0 0 41 145 38 601.0 0 38 146 44 601.0 0 44 147 44 601.0 0 44 148 67 601.0 0 67 149 25 601.0 0 25 150 32 601.0 0 32 151 31 601.0 0 31 152 55 601.0 0 55 153 40 601.0 0 40 154 51 601.0 0 51 155 50 601.0 0 50 156 57 601.0 0 57 157 37 601.0 0 37 158 90 601.0 0 90 159 27 601.0 0 27 160 35 601.0 0 35 161 44 601.0 0 44 162 57 601.0 0 57 163 51 601.0 0 51 164 69 601.0 0 69 165 38 601.0 0 38 166 33 601.0 0 33 167 88 601.0 0 88 168 53 601.0 0 53 169 81 601.0 0 81 170 44 601.0 0 44 171 40 601.0 0 40 172 47 601.0 0 47 173 73 601.0 0 73 174 58 601.0 0 58 175 52 601.0 0 52 176 46 601.0 0 46 177 37 601.0 0 37 178 173 601.0 0 173 179 167 601.0 0 167 180 127 601.0 0 127 181 67 601.0 0 67 182 45 601.0 0 45 183 41 601.0 0 41 184 282 601.0 0 282 185 61 601.0 0 61 186 89 601.0 0 89 187 44 601.0 0 44 188 73 601.0 0 73 189 45 601.0 0 45 190 52 601.0 0 52 191 49 601.0 0 49 192 47 601.0 0 47 193 51 601.0 0 51 194 71 601.0 0 71 195 94 601.0 0 94 196 50 601.0 0 50 197 61 601.0 0 61 198 142 601.0 0 142 199 195 601.0 0 195 200 167 601.0 0 167 201 87 601.0 0 87 202 59 601.0 0 59 203 45 601.0 0 45 204 55 601.0 0 55 205 79 601.0 0 79 206 64 601.0 0 64 207 103 601.0 0 103 208 72 601.0 0 72 209 44 601.0 0 44 210 50 601.0 0 50 211 58 601.0 0 58 212 34 601.0 0 34 213 32 601.0 0 32 214 46 601.0 0 46 215 62 601.0 0 62 216 44 601.0 0 44 217 39 601.0 0 39 218 69 601.0 0 69 219 116 601.0 0 116 220 128 601.0 0 128 221 161 601.0 0 161 222 431 601.0 0 431 223 391 601.0 0 391 224 2623 601.0 0 2623 225 92 601.0 0 92 226 63 601.0 0 63 227 59 601.0 0 59 228 43 601.0 0 43 229 52 601.0 0 52 230 60 601.0 0 60 231 48 601.0 0 48 232 36 601.0 0 36 233 50 601.0 0 50 234 56 601.0 0 56 235 38 601.0 0 38 236 38 601.0 0 38 237 30 601.0 0 30 238 81 601.0 0 81 239 104 601.0 0 104 240 486 601.0 0 486 241 803 601.0 0 803 242 774 601.0 0 774 243 68 601.0 0 68 244 68 601.0 0 68 245 32 601.0 0 32 246 49 601.0 0 49 247 56 601.0 0 56 248 58 601.0 0 58 249 51 601.0 0 51 250 73 601.0 0 73 251 86 601.0 0 86 252 94 601.0 0 94 253 63 601.0 0 63 254 40 601.0 0 40 255 57 601.0 0 57 256 52 601.0 0 52 257 45 601.0 0 45 258 47 601.0 0 47 259 41 601.0 0 41 260 67 601.0 0 67 261 55 601.0 0 55 262 135 601.0 0 135 263 85 601.0 0 85 264 42 601.0 0 42 265 44 601.0 0 44 266 47 601.0 0 47 267 52 601.0 0 52 268 43 601.0 0 43 269 59 601.0 0 59 270 45 601.0 0 45 271 62 601.0 0 62 272 54 601.0 0 54 273 43 601.0 0 43 274 49 601.0 0 49 275 43 601.0 0 43 276 44 601.0 0 44 277 57 601.0 0 57 278 53 601.0 0 53 279 64 601.0 0 64 280 43 601.0 0 43 281 43 601.0 0 43 282 43 601.0 0 43 283 48 601.0 0 48 284 69 601.0 0 69 285 48 601.0 0 48 286 75 601.0 0 75 287 77 601.0 0 77 288 158 601.0 0 158 289 170 601.0 0 170 290 152 601.0 0 152 291 93 601.0 0 93 292 73 601.0 0 73 293 76 601.0 0 76 294 54 601.0 0 54 295 58 601.0 0 58 296 49 601.0 0 49 297 63 601.0 0 63 298 68 601.0 0 68 299 61 601.0 0 61 300 85 601.0 0 85 301 66 601.0 0 66 Finished in 3.15 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 61,582 Reads with adapters: 26,266 (42.7%) Reads that were too short: 24 (0.0%) Reads written (passing filters): 26,242 (42.6%) Total basepairs processed: 16,556,396 bp Total written (filtered): 7,183,798 bp (43.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 26266 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 7 0.0 3 0 0 7 19 300 0.0 3 0 296 3 0 1 20 25794 0.0 4 25442 338 11 2 1 21 45 0.0 4 8 36 1 22 2 0.0 4 0 0 2 23 22 0.0 4 0 0 0 18 4 24 1 0.0 4 0 0 0 0 1 27 3 0.0 4 0 0 0 0 3 28 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 86 8 0.0 4 0 0 0 0 8 96 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 50 0.0 4 0 0 0 0 50 129 1 0.0 4 0 0 0 0 1 135 1 0.0 4 1 193 1 0.0 4 0 0 0 0 1 194 13 0.0 4 0 0 0 0 13 201 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 254 1 0.0 4 1 262 1 0.0 4 0 1 275 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.91 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 26,242 Reads with adapters: 311 (1.2%) Reads written (passing filters): 26,242 (100.0%) Total basepairs processed: 7,183,798 bp Total written (filtered): 7,178,242 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 311 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 15.4% C: 8.4% G: 73.3% T: 2.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 43 410.0 0 43 4 5 102.5 0 5 10 11 0.0 1 0 11 11 78 0.0 1 2 76 12 77 0.0 1 13 64 14 2 0.0 1 1 1 15 72 0.0 1 48 24 16 2 0.0 1 2 20 4 0.0 1 3 1 25 2 0.0 1 2 33 1 0.0 1 1 79 3 0.0 1 3 109 1 0.0 1 1 111 1 0.0 1 1 118 1 0.0 1 1 152 1 0.0 1 0 1 210 6 0.0 1 3 3 225 1 0.0 1 1