This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.61 s (27 us/read; 2.18 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 317,566 (12.9%) Reads that were too short: 36,146 (1.5%) Reads written (passing filters): 281,420 (11.4%) Total basepairs processed: 740,966,583 bp Total written (filtered): 76,178,416 bp (10.3%) === Adapter 1 === Sequence: AGCTGG; Type: regular 5'; Length: 6; Trimmed: 317566 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2407 601.0 0 2407 7 54150 601.0 0 54150 8 2307 601.0 0 2307 9 29467 601.0 0 29467 10 4280 601.0 0 4280 11 131689 601.0 0 131689 12 269 601.0 0 269 13 113 601.0 0 113 14 90 601.0 0 90 15 105 601.0 0 105 16 85 601.0 0 85 17 69 601.0 0 69 18 76 601.0 0 76 19 99 601.0 0 99 20 125 601.0 0 125 21 72 601.0 0 72 22 78 601.0 0 78 23 69 601.0 0 69 24 71 601.0 0 71 25 92 601.0 0 92 26 110 601.0 0 110 27 118 601.0 0 118 28 288 601.0 0 288 29 123 601.0 0 123 30 281 601.0 0 281 31 84 601.0 0 84 32 99 601.0 0 99 33 78 601.0 0 78 34 112 601.0 0 112 35 61 601.0 0 61 36 94 601.0 0 94 37 110 601.0 0 110 38 89 601.0 0 89 39 105 601.0 0 105 40 104 601.0 0 104 41 152 601.0 0 152 42 101 601.0 0 101 43 67 601.0 0 67 44 103 601.0 0 103 45 83 601.0 0 83 46 95 601.0 0 95 47 94 601.0 0 94 48 109 601.0 0 109 49 130 601.0 0 130 50 218 601.0 0 218 51 97 601.0 0 97 52 96 601.0 0 96 53 100 601.0 0 100 54 92 601.0 0 92 55 94 601.0 0 94 56 124 601.0 0 124 57 100 601.0 0 100 58 87 601.0 0 87 59 75 601.0 0 75 60 102 601.0 0 102 61 114 601.0 0 114 62 138 601.0 0 138 63 103 601.0 0 103 64 97 601.0 0 97 65 92 601.0 0 92 66 85 601.0 0 85 67 99 601.0 0 99 68 89 601.0 0 89 69 118 601.0 0 118 70 399 601.0 0 399 71 111 601.0 0 111 72 350 601.0 0 350 73 99 601.0 0 99 74 86 601.0 0 86 75 95 601.0 0 95 76 115 601.0 0 115 77 114 601.0 0 114 78 124 601.0 0 124 79 300 601.0 0 300 80 459 601.0 0 459 81 724 601.0 0 724 82 84 601.0 0 84 83 251 601.0 0 251 84 692 601.0 0 692 85 1209 601.0 0 1209 86 97 601.0 0 97 87 83 601.0 0 83 88 80 601.0 0 80 89 133 601.0 0 133 90 114 601.0 0 114 91 73 601.0 0 73 92 84 601.0 0 84 93 96 601.0 0 96 94 72 601.0 0 72 95 106 601.0 0 106 96 104 601.0 0 104 97 251 601.0 0 251 98 202 601.0 0 202 99 756 601.0 0 756 100 451 601.0 0 451 101 127 601.0 0 127 102 194 601.0 0 194 103 139 601.0 0 139 104 109 601.0 0 109 105 177 601.0 0 177 106 350 601.0 0 350 107 200 601.0 0 200 108 181 601.0 0 181 109 207 601.0 0 207 110 321 601.0 0 321 111 351 601.0 0 351 112 695 601.0 0 695 113 14401 601.0 0 14401 114 7059 601.0 0 7059 115 2757 601.0 0 2757 116 127 601.0 0 127 117 86 601.0 0 86 118 117 601.0 0 117 119 125 601.0 0 125 120 93 601.0 0 93 121 92 601.0 0 92 122 95 601.0 0 95 123 119 601.0 0 119 124 196 601.0 0 196 125 158 601.0 0 158 126 139 601.0 0 139 127 196 601.0 0 196 128 268 601.0 0 268 129 1116 601.0 0 1116 130 1012 601.0 0 1012 131 1163 601.0 0 1163 132 103 601.0 0 103 133 166 601.0 0 166 134 77 601.0 0 77 135 274 601.0 0 274 136 114 601.0 0 114 137 154 601.0 0 154 138 161 601.0 0 161 139 315 601.0 0 315 140 289 601.0 0 289 141 184 601.0 0 184 142 351 601.0 0 351 143 413 601.0 0 413 144 132 601.0 0 132 145 115 601.0 0 115 146 146 601.0 0 146 147 70 601.0 0 70 148 124 601.0 0 124 149 97 601.0 0 97 150 102 601.0 0 102 151 71 601.0 0 71 152 94 601.0 0 94 153 107 601.0 0 107 154 381 601.0 0 381 155 538 601.0 0 538 156 432 601.0 0 432 157 390 601.0 0 390 158 198 601.0 0 198 159 93 601.0 0 93 160 82 601.0 0 82 161 82 601.0 0 82 162 86 601.0 0 86 163 119 601.0 0 119 164 84 601.0 0 84 165 118 601.0 0 118 166 73 601.0 0 73 167 260 601.0 0 260 168 256 601.0 0 256 169 148 601.0 0 148 170 307 601.0 0 307 171 87 601.0 0 87 172 103 601.0 0 103 173 74 601.0 0 74 174 72 601.0 0 72 175 89 601.0 0 89 176 91 601.0 0 91 177 77 601.0 0 77 178 89 601.0 0 89 179 193 601.0 0 193 180 62 601.0 0 62 181 71 601.0 0 71 182 70 601.0 0 70 183 93 601.0 0 93 184 85 601.0 0 85 185 95 601.0 0 95 186 90 601.0 0 90 187 97 601.0 0 97 188 91 601.0 0 91 189 100 601.0 0 100 190 120 601.0 0 120 191 112 601.0 0 112 192 122 601.0 0 122 193 76 601.0 0 76 194 81 601.0 0 81 195 86 601.0 0 86 196 148 601.0 0 148 197 100 601.0 0 100 198 93 601.0 0 93 199 89 601.0 0 89 200 325 601.0 0 325 201 126 601.0 0 126 202 183 601.0 0 183 203 87 601.0 0 87 204 107 601.0 0 107 205 123 601.0 0 123 206 100 601.0 0 100 207 77 601.0 0 77 208 75 601.0 0 75 209 121 601.0 0 121 210 134 601.0 0 134 211 399 601.0 0 399 212 233 601.0 0 233 213 263 601.0 0 263 214 151 601.0 0 151 215 135 601.0 0 135 216 233 601.0 0 233 217 124 601.0 0 124 218 102 601.0 0 102 219 66 601.0 0 66 220 80 601.0 0 80 221 84 601.0 0 84 222 89 601.0 0 89 223 80 601.0 0 80 224 93 601.0 0 93 225 119 601.0 0 119 226 112 601.0 0 112 227 110 601.0 0 110 228 979 601.0 0 979 229 643 601.0 0 643 230 378 601.0 0 378 231 154 601.0 0 154 232 169 601.0 0 169 233 145 601.0 0 145 234 233 601.0 0 233 235 335 601.0 0 335 236 400 601.0 0 400 237 212 601.0 0 212 238 97 601.0 0 97 239 95 601.0 0 95 240 117 601.0 0 117 241 119 601.0 0 119 242 107 601.0 0 107 243 182 601.0 0 182 244 102 601.0 0 102 245 98 601.0 0 98 246 181 601.0 0 181 247 281 601.0 0 281 248 261 601.0 0 261 249 171 601.0 0 171 250 135 601.0 0 135 251 85 601.0 0 85 252 141 601.0 0 141 253 127 601.0 0 127 254 124 601.0 0 124 255 199 601.0 0 199 256 194 601.0 0 194 257 336 601.0 0 336 258 157 601.0 0 157 259 115 601.0 0 115 260 71 601.0 0 71 261 119 601.0 0 119 262 149 601.0 0 149 263 132 601.0 0 132 264 121 601.0 0 121 265 109 601.0 0 109 266 63 601.0 0 63 267 94 601.0 0 94 268 261 601.0 0 261 269 394 601.0 0 394 270 384 601.0 0 384 271 235 601.0 0 235 272 158 601.0 0 158 273 232 601.0 0 232 274 242 601.0 0 242 275 131 601.0 0 131 276 158 601.0 0 158 277 74 601.0 0 74 278 171 601.0 0 171 279 179 601.0 0 179 280 134 601.0 0 134 281 348 601.0 0 348 282 205 601.0 0 205 283 177 601.0 0 177 284 176 601.0 0 176 285 173 601.0 0 173 286 170 601.0 0 170 287 140 601.0 0 140 288 217 601.0 0 217 289 188 601.0 0 188 290 195 601.0 0 195 291 195 601.0 0 195 292 203 601.0 0 203 293 260 601.0 0 260 294 773 601.0 0 773 295 1262 601.0 0 1262 296 3142 601.0 0 3142 297 2725 601.0 0 2725 298 4013 601.0 0 4013 299 4328 601.0 0 4328 300 2782 601.0 0 2782 301 311 601.0 0 311 Finished in 12.15 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 281,420 Reads with adapters: 53,535 (19.0%) Reads that were too short: 521 (0.2%) Reads written (passing filters): 53,014 (18.8%) Total basepairs processed: 76,178,416 bp Total written (filtered): 14,625,868 bp (19.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 53535 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 8 0.0 2 0 0 0 8 15 40 0.0 3 0 0 21 19 16 398 0.0 3 0 280 75 43 17 51569 0.0 3 47163 3484 701 221 18 195 0.0 3 16 94 21 64 19 156 0.0 3 0 0 97 59 20 244 0.0 3 0 0 0 244 21 2 0.0 3 1 0 0 1 23 27 0.0 3 1 0 0 26 29 1 0.0 3 1 31 1 0.0 3 0 0 0 1 32 59 0.0 3 0 0 1 58 33 1 0.0 3 1 34 4 0.0 3 0 0 0 4 35 10 0.0 3 0 0 0 10 37 1 0.0 3 0 0 0 1 38 2 0.0 3 0 0 0 2 39 5 0.0 3 0 0 0 5 40 2 0.0 3 0 0 0 2 43 2 0.0 3 0 0 0 2 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 3 0.0 3 0 0 0 3 50 21 0.0 3 0 0 0 21 52 1 0.0 3 0 0 0 1 53 54 0.0 3 0 0 1 53 54 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 56 11 0.0 3 0 0 0 11 57 1 0.0 3 0 0 0 1 58 15 0.0 3 0 0 1 14 59 5 0.0 3 0 0 0 5 61 5 0.0 3 0 0 0 5 62 9 0.0 3 0 0 0 9 63 3 0.0 3 0 0 0 3 64 6 0.0 3 0 0 1 5 65 7 0.0 3 0 0 2 5 66 2 0.0 3 0 0 0 2 68 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 73 23 0.0 3 0 0 18 5 74 2 0.0 3 0 0 0 2 77 19 0.0 3 0 0 0 19 78 4 0.0 3 0 0 0 4 79 11 0.0 3 0 0 0 11 80 2 0.0 3 0 0 1 1 81 1 0.0 3 0 0 0 1 82 2 0.0 3 0 0 1 1 83 6 0.0 3 0 0 0 6 85 3 0.0 3 0 0 0 3 86 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 94 7 0.0 3 0 0 0 7 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 104 26 0.0 3 0 0 2 24 105 2 0.0 3 0 0 0 2 106 2 0.0 3 0 0 0 2 109 2 0.0 3 0 1 0 1 111 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 113 7 0.0 3 0 0 0 7 114 1 0.0 3 0 0 0 1 115 3 0.0 3 0 0 0 3 116 1 0.0 3 0 0 0 1 117 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 1 120 3 0.0 3 0 0 0 3 121 2 0.0 3 0 0 0 2 122 14 0.0 3 0 0 1 13 123 1 0.0 3 0 0 0 1 124 5 0.0 3 0 0 0 5 125 19 0.0 3 0 1 0 18 126 3 0.0 3 0 0 1 2 127 1 0.0 3 0 0 0 1 129 3 0.0 3 0 0 0 3 130 1 0.0 3 0 0 0 1 131 3 0.0 3 0 0 0 3 132 4 0.0 3 0 0 0 4 133 2 0.0 3 0 0 0 2 134 2 0.0 3 0 0 0 2 135 3 0.0 3 0 0 0 3 137 2 0.0 3 0 0 0 2 138 2 0.0 3 1 0 0 1 143 1 0.0 3 0 0 0 1 144 3 0.0 3 0 0 0 3 146 2 0.0 3 0 0 0 2 147 4 0.0 3 0 0 0 4 149 5 0.0 3 0 0 0 5 151 4 0.0 3 0 0 0 4 153 15 0.0 3 0 0 2 13 154 21 0.0 3 0 0 0 21 155 4 0.0 3 0 0 0 4 156 2 0.0 3 0 0 0 2 158 1 0.0 3 0 0 0 1 159 1 0.0 3 1 160 1 0.0 3 0 0 0 1 161 2 0.0 3 0 0 0 2 162 2 0.0 3 0 0 0 2 163 1 0.0 3 0 0 0 1 164 12 0.0 3 0 0 1 11 165 4 0.0 3 0 0 0 4 166 3 0.0 3 0 0 0 3 168 3 0.0 3 0 0 0 3 170 3 0.0 3 0 0 0 3 171 1 0.0 3 0 0 0 1 172 2 0.0 3 0 0 0 2 174 3 0.0 3 0 0 1 2 176 4 0.0 3 0 0 0 4 177 1 0.0 3 0 0 0 1 178 16 0.0 3 0 0 0 16 179 2 0.0 3 0 0 0 2 180 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 1 1 183 3 0.0 3 0 0 0 3 184 2 0.0 3 0 0 0 2 185 2 0.0 3 0 0 0 2 186 2 0.0 3 0 0 0 2 187 4 0.0 3 0 0 1 3 188 3 0.0 3 0 0 2 1 189 1 0.0 3 0 0 0 1 190 9 0.0 3 0 0 0 9 191 2 0.0 3 0 0 0 2 192 13 0.0 3 0 0 0 13 193 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 199 1 0.0 3 0 0 0 1 200 31 0.0 3 0 0 0 31 202 1 0.0 3 0 0 0 1 203 29 0.0 3 0 0 0 29 204 3 0.0 3 0 0 0 3 205 2 0.0 3 0 0 0 2 206 3 0.0 3 0 0 0 3 207 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 214 3 0.0 3 0 0 0 3 215 4 0.0 3 0 0 0 4 216 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 0 2 219 3 0.0 3 0 0 0 3 221 1 0.0 3 0 0 0 1 223 2 0.0 3 0 0 0 2 224 2 0.0 3 0 0 0 2 228 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 234 2 0.0 3 0 0 0 2 235 1 0.0 3 0 0 0 1 236 10 0.0 3 0 0 0 10 237 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 250 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 275 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 0 1 277 22 0.0 3 0 0 0 22 278 21 0.0 3 0 0 1 20 279 40 0.0 3 0 0 0 40 280 33 0.0 3 0 0 2 31 281 21 0.0 3 0 0 2 19 282 5 0.0 3 0 0 0 5 283 4 0.0 3 0 0 0 4 284 1 0.0 3 0 0 0 1 285 2 0.0 3 0 0 0 2 286 6 0.0 3 0 0 0 6 289 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.84 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 53,014 Reads with adapters: 313 (0.6%) Reads written (passing filters): 53,014 (100.0%) Total basepairs processed: 14,625,868 bp Total written (filtered): 14,624,866 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 313 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 36.1% C: 27.5% G: 26.5% T: 9.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 276 828.3 0 276 4 22 207.1 0 22 5 8 51.8 0 8 6 5 12.9 0 5 8 2 0.8 0 2