This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCTGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.46 s (28 us/read; 2.16 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 348,190 (14.1%) Reads that were too short: 27,831 (1.1%) Reads written (passing filters): 320,359 (13.0%) Total basepairs processed: 740,966,583 bp Total written (filtered): 87,686,444 bp (11.8%) === Adapter 1 === Sequence: AGCTGG; Type: regular 5'; Length: 6; Trimmed: 348190 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2280 601.0 0 2280 7 45645 601.0 0 45645 8 2004 601.0 0 2004 9 41411 601.0 0 41411 10 3547 601.0 0 3547 11 171223 601.0 0 171223 12 254 601.0 0 254 13 92 601.0 0 92 14 82 601.0 0 82 15 83 601.0 0 83 16 65 601.0 0 65 17 72 601.0 0 72 18 66 601.0 0 66 19 73 601.0 0 73 20 80 601.0 0 80 21 60 601.0 0 60 22 71 601.0 0 71 23 67 601.0 0 67 24 78 601.0 0 78 25 72 601.0 0 72 26 87 601.0 0 87 27 69 601.0 0 69 28 99 601.0 0 99 29 90 601.0 0 90 30 266 601.0 0 266 31 76 601.0 0 76 32 71 601.0 0 71 33 79 601.0 0 79 34 89 601.0 0 89 35 82 601.0 0 82 36 101 601.0 0 101 37 95 601.0 0 95 38 80 601.0 0 80 39 129 601.0 0 129 40 93 601.0 0 93 41 199 601.0 0 199 42 122 601.0 0 122 43 71 601.0 0 71 44 101 601.0 0 101 45 83 601.0 0 83 46 111 601.0 0 111 47 97 601.0 0 97 48 119 601.0 0 119 49 120 601.0 0 120 50 229 601.0 0 229 51 82 601.0 0 82 52 88 601.0 0 88 53 103 601.0 0 103 54 89 601.0 0 89 55 126 601.0 0 126 56 97 601.0 0 97 57 99 601.0 0 99 58 74 601.0 0 74 59 90 601.0 0 90 60 80 601.0 0 80 61 98 601.0 0 98 62 116 601.0 0 116 63 118 601.0 0 118 64 118 601.0 0 118 65 101 601.0 0 101 66 91 601.0 0 91 67 80 601.0 0 80 68 94 601.0 0 94 69 95 601.0 0 95 70 400 601.0 0 400 71 130 601.0 0 130 72 381 601.0 0 381 73 87 601.0 0 87 74 101 601.0 0 101 75 85 601.0 0 85 76 89 601.0 0 89 77 90 601.0 0 90 78 101 601.0 0 101 79 202 601.0 0 202 80 464 601.0 0 464 81 434 601.0 0 434 82 107 601.0 0 107 83 277 601.0 0 277 84 824 601.0 0 824 85 1387 601.0 0 1387 86 90 601.0 0 90 87 93 601.0 0 93 88 98 601.0 0 98 89 109 601.0 0 109 90 91 601.0 0 91 91 94 601.0 0 94 92 91 601.0 0 91 93 91 601.0 0 91 94 94 601.0 0 94 95 90 601.0 0 90 96 94 601.0 0 94 97 197 601.0 0 197 98 183 601.0 0 183 99 738 601.0 0 738 100 449 601.0 0 449 101 158 601.0 0 158 102 185 601.0 0 185 103 156 601.0 0 156 104 134 601.0 0 134 105 172 601.0 0 172 106 338 601.0 0 338 107 188 601.0 0 188 108 224 601.0 0 224 109 191 601.0 0 191 110 301 601.0 0 301 111 272 601.0 0 272 112 473 601.0 0 473 113 13803 601.0 0 13803 114 6129 601.0 0 6129 115 2630 601.0 0 2630 116 142 601.0 0 142 117 75 601.0 0 75 118 156 601.0 0 156 119 102 601.0 0 102 120 91 601.0 0 91 121 81 601.0 0 81 122 95 601.0 0 95 123 137 601.0 0 137 124 166 601.0 0 166 125 143 601.0 0 143 126 122 601.0 0 122 127 125 601.0 0 125 128 188 601.0 0 188 129 1094 601.0 0 1094 130 858 601.0 0 858 131 1158 601.0 0 1158 132 109 601.0 0 109 133 115 601.0 0 115 134 92 601.0 0 92 135 217 601.0 0 217 136 92 601.0 0 92 137 105 601.0 0 105 138 91 601.0 0 91 139 133 601.0 0 133 140 117 601.0 0 117 141 81 601.0 0 81 142 254 601.0 0 254 143 344 601.0 0 344 144 122 601.0 0 122 145 75 601.0 0 75 146 139 601.0 0 139 147 86 601.0 0 86 148 112 601.0 0 112 149 82 601.0 0 82 150 119 601.0 0 119 151 60 601.0 0 60 152 95 601.0 0 95 153 134 601.0 0 134 154 444 601.0 0 444 155 709 601.0 0 709 156 587 601.0 0 587 157 487 601.0 0 487 158 273 601.0 0 273 159 104 601.0 0 104 160 86 601.0 0 86 161 67 601.0 0 67 162 82 601.0 0 82 163 139 601.0 0 139 164 83 601.0 0 83 165 128 601.0 0 128 166 107 601.0 0 107 167 333 601.0 0 333 168 268 601.0 0 268 169 129 601.0 0 129 170 441 601.0 0 441 171 135 601.0 0 135 172 102 601.0 0 102 173 105 601.0 0 105 174 104 601.0 0 104 175 95 601.0 0 95 176 83 601.0 0 83 177 88 601.0 0 88 178 105 601.0 0 105 179 223 601.0 0 223 180 80 601.0 0 80 181 71 601.0 0 71 182 83 601.0 0 83 183 80 601.0 0 80 184 100 601.0 0 100 185 91 601.0 0 91 186 93 601.0 0 93 187 107 601.0 0 107 188 96 601.0 0 96 189 120 601.0 0 120 190 95 601.0 0 95 191 92 601.0 0 92 192 92 601.0 0 92 193 87 601.0 0 87 194 111 601.0 0 111 195 79 601.0 0 79 196 97 601.0 0 97 197 102 601.0 0 102 198 100 601.0 0 100 199 104 601.0 0 104 200 305 601.0 0 305 201 123 601.0 0 123 202 161 601.0 0 161 203 88 601.0 0 88 204 97 601.0 0 97 205 134 601.0 0 134 206 96 601.0 0 96 207 72 601.0 0 72 208 81 601.0 0 81 209 101 601.0 0 101 210 108 601.0 0 108 211 664 601.0 0 664 212 110 601.0 0 110 213 118 601.0 0 118 214 92 601.0 0 92 215 77 601.0 0 77 216 178 601.0 0 178 217 178 601.0 0 178 218 105 601.0 0 105 219 93 601.0 0 93 220 85 601.0 0 85 221 99 601.0 0 99 222 115 601.0 0 115 223 109 601.0 0 109 224 92 601.0 0 92 225 119 601.0 0 119 226 107 601.0 0 107 227 92 601.0 0 92 228 346 601.0 0 346 229 239 601.0 0 239 230 178 601.0 0 178 231 140 601.0 0 140 232 125 601.0 0 125 233 102 601.0 0 102 234 127 601.0 0 127 235 177 601.0 0 177 236 184 601.0 0 184 237 134 601.0 0 134 238 58 601.0 0 58 239 121 601.0 0 121 240 92 601.0 0 92 241 91 601.0 0 91 242 98 601.0 0 98 243 123 601.0 0 123 244 86 601.0 0 86 245 80 601.0 0 80 246 75 601.0 0 75 247 118 601.0 0 118 248 162 601.0 0 162 249 112 601.0 0 112 250 155 601.0 0 155 251 66 601.0 0 66 252 95 601.0 0 95 253 90 601.0 0 90 254 83 601.0 0 83 255 104 601.0 0 104 256 113 601.0 0 113 257 207 601.0 0 207 258 116 601.0 0 116 259 87 601.0 0 87 260 75 601.0 0 75 261 108 601.0 0 108 262 95 601.0 0 95 263 115 601.0 0 115 264 168 601.0 0 168 265 107 601.0 0 107 266 89 601.0 0 89 267 118 601.0 0 118 268 156 601.0 0 156 269 195 601.0 0 195 270 183 601.0 0 183 271 140 601.0 0 140 272 116 601.0 0 116 273 86 601.0 0 86 274 117 601.0 0 117 275 86 601.0 0 86 276 168 601.0 0 168 277 84 601.0 0 84 278 181 601.0 0 181 279 151 601.0 0 151 280 111 601.0 0 111 281 117 601.0 0 117 282 114 601.0 0 114 283 128 601.0 0 128 284 132 601.0 0 132 285 161 601.0 0 161 286 104 601.0 0 104 287 95 601.0 0 95 288 130 601.0 0 130 289 145 601.0 0 145 290 141 601.0 0 141 291 155 601.0 0 155 292 139 601.0 0 139 293 147 601.0 0 147 294 388 601.0 0 388 295 662 601.0 0 662 296 1545 601.0 0 1545 297 1038 601.0 0 1038 298 2684 601.0 0 2684 299 5805 601.0 0 5805 300 3736 601.0 0 3736 301 161 601.0 0 161 Finished in 15.95 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 320,359 Reads with adapters: 42,002 (13.1%) Reads that were too short: 48 (0.0%) Reads written (passing filters): 41,954 (13.1%) Total basepairs processed: 87,686,444 bp Total written (filtered): 11,410,718 bp (13.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 42002 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 19 0.0 3 0 0 19 19 463 0.0 3 0 458 5 20 41283 0.0 4 40662 594 24 2 1 21 89 0.0 4 21 65 2 1 22 11 0.0 4 1 0 9 1 23 50 0.0 4 1 1 0 40 8 24 4 0.0 4 2 0 0 0 2 25 2 0.0 4 1 0 0 0 1 26 2 0.0 4 0 0 1 0 1 27 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 0 1 35 1 0.0 4 0 0 0 0 1 40 1 0.0 4 1 44 1 0.0 4 1 45 1 0.0 4 0 0 0 0 1 75 1 0.0 4 1 88 1 0.0 4 0 1 94 2 0.0 4 2 96 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 101 1 0.0 4 0 1 105 1 0.0 4 1 113 2 0.0 4 0 0 0 0 2 115 1 0.0 4 1 121 8 0.0 4 0 0 0 0 8 122 2 0.0 4 1 0 1 123 1 0.0 4 0 0 0 0 1 124 2 0.0 4 2 127 1 0.0 4 0 0 0 0 1 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 1 130 2 0.0 4 2 136 2 0.0 4 2 151 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 160 1 0.0 4 1 167 2 0.0 4 2 169 3 0.0 4 0 0 0 0 3 170 1 0.0 4 1 171 1 0.0 4 1 175 1 0.0 4 1 176 1 0.0 4 1 177 2 0.0 4 1 0 1 197 1 0.0 4 0 0 1 203 1 0.0 4 1 209 1 0.0 4 0 0 0 0 1 210 2 0.0 4 1 0 0 0 1 219 1 0.0 4 0 1 221 1 0.0 4 0 0 0 0 1 224 1 0.0 4 1 226 1 0.0 4 1 228 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 1 232 1 0.0 4 0 0 0 0 1 233 1 0.0 4 1 234 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 251 1 0.0 4 1 252 1 0.0 4 0 0 1 257 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 266 1 0.0 4 0 1 273 1 0.0 4 0 0 0 0 1 280 1 0.0 4 0 0 0 0 1 292 2 0.0 4 0 0 0 0 2 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.49 s (36 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 41,954 Reads with adapters: 6,295 (15.0%) Reads written (passing filters): 41,954 (100.0%) Total basepairs processed: 11,410,718 bp Total written (filtered): 11,344,505 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6295 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.0% C: 3.1% G: 94.2% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 10 655.5 0 10 4 13 163.9 0 13 5 3 41.0 0 3 6 5 10.2 0 5 8 2 0.6 0 2 9 3041 0.2 0 97 2944 10 2830 0.0 1 103 2727 11 321 0.0 1 1 320 12 5 0.0 1 0 5 13 14 0.0 1 0 14 18 1 0.0 1 1 22 6 0.0 1 5 1 81 1 0.0 1 1 83 1 0.0 1 1 94 4 0.0 1 4 100 1 0.0 1 1 104 1 0.0 1 1 105 1 0.0 1 1 107 1 0.0 1 0 1 115 1 0.0 1 1 120 16 0.0 1 14 2 125 1 0.0 1 1 150 1 0.0 1 0 1 155 1 0.0 1 1 195 1 0.0 1 1 208 4 0.0 1 2 2 210 1 0.0 1 0 1 211 3 0.0 1 2 1 226 1 0.0 1 1 234 1 0.0 1 1 241 2 0.0 1 2 245 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.