This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GATATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.83 s (26 us/read; 2.28 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 122,429 (5.0%) Reads that were too short: 20,900 (0.8%) Reads written (passing filters): 101,529 (4.1%) Total basepairs processed: 740,966,583 bp Total written (filtered): 26,949,513 bp (3.6%) === Adapter 1 === Sequence: GATATT; Type: regular 5'; Length: 6; Trimmed: 122429 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 616 601.0 0 616 7 37855 601.0 0 37855 8 1057 601.0 0 1057 9 31856 601.0 0 31856 10 188 601.0 0 188 11 166 601.0 0 166 12 159 601.0 0 159 13 145 601.0 0 145 14 134 601.0 0 134 15 184 601.0 0 184 16 175 601.0 0 175 17 156 601.0 0 156 18 146 601.0 0 146 19 156 601.0 0 156 20 136 601.0 0 136 21 165 601.0 0 165 22 172 601.0 0 172 23 154 601.0 0 154 24 160 601.0 0 160 25 181 601.0 0 181 26 171 601.0 0 171 27 154 601.0 0 154 28 145 601.0 0 145 29 135 601.0 0 135 30 140 601.0 0 140 31 131 601.0 0 131 32 141 601.0 0 141 33 169 601.0 0 169 34 204 601.0 0 204 35 978 601.0 0 978 36 743 601.0 0 743 37 329 601.0 0 329 38 122 601.0 0 122 39 129 601.0 0 129 40 131 601.0 0 131 41 159 601.0 0 159 42 129 601.0 0 129 43 127 601.0 0 127 44 125 601.0 0 125 45 105 601.0 0 105 46 120 601.0 0 120 47 135 601.0 0 135 48 125 601.0 0 125 49 172 601.0 0 172 50 126 601.0 0 126 51 174 601.0 0 174 52 134 601.0 0 134 53 119 601.0 0 119 54 116 601.0 0 116 55 121 601.0 0 121 56 136 601.0 0 136 57 106 601.0 0 106 58 121 601.0 0 121 59 114 601.0 0 114 60 196 601.0 0 196 61 134 601.0 0 134 62 132 601.0 0 132 63 130 601.0 0 130 64 156 601.0 0 156 65 139 601.0 0 139 66 122 601.0 0 122 67 118 601.0 0 118 68 122 601.0 0 122 69 129 601.0 0 129 70 170 601.0 0 170 71 130 601.0 0 130 72 163 601.0 0 163 73 127 601.0 0 127 74 120 601.0 0 120 75 107 601.0 0 107 76 135 601.0 0 135 77 118 601.0 0 118 78 135 601.0 0 135 79 145 601.0 0 145 80 148 601.0 0 148 81 148 601.0 0 148 82 128 601.0 0 128 83 121 601.0 0 121 84 135 601.0 0 135 85 142 601.0 0 142 86 114 601.0 0 114 87 104 601.0 0 104 88 252 601.0 0 252 89 119 601.0 0 119 90 168 601.0 0 168 91 143 601.0 0 143 92 125 601.0 0 125 93 124 601.0 0 124 94 154 601.0 0 154 95 155 601.0 0 155 96 133 601.0 0 133 97 154 601.0 0 154 98 128 601.0 0 128 99 187 601.0 0 187 100 322 601.0 0 322 101 317 601.0 0 317 102 141 601.0 0 141 103 180 601.0 0 180 104 149 601.0 0 149 105 157 601.0 0 157 106 155 601.0 0 155 107 143 601.0 0 143 108 132 601.0 0 132 109 142 601.0 0 142 110 182 601.0 0 182 111 335 601.0 0 335 112 328 601.0 0 328 113 313 601.0 0 313 114 142 601.0 0 142 115 118 601.0 0 118 116 120 601.0 0 120 117 136 601.0 0 136 118 148 601.0 0 148 119 125 601.0 0 125 120 133 601.0 0 133 121 198 601.0 0 198 122 375 601.0 0 375 123 185 601.0 0 185 124 159 601.0 0 159 125 116 601.0 0 116 126 115 601.0 0 115 127 116 601.0 0 116 128 106 601.0 0 106 129 137 601.0 0 137 130 148 601.0 0 148 131 150 601.0 0 150 132 144 601.0 0 144 133 131 601.0 0 131 134 125 601.0 0 125 135 104 601.0 0 104 136 130 601.0 0 130 137 116 601.0 0 116 138 120 601.0 0 120 139 239 601.0 0 239 140 124 601.0 0 124 141 205 601.0 0 205 142 123 601.0 0 123 143 214 601.0 0 214 144 127 601.0 0 127 145 133 601.0 0 133 146 123 601.0 0 123 147 182 601.0 0 182 148 131 601.0 0 131 149 146 601.0 0 146 150 92 601.0 0 92 151 125 601.0 0 125 152 124 601.0 0 124 153 135 601.0 0 135 154 124 601.0 0 124 155 134 601.0 0 134 156 143 601.0 0 143 157 143 601.0 0 143 158 131 601.0 0 131 159 368 601.0 0 368 160 404 601.0 0 404 161 268 601.0 0 268 162 99 601.0 0 99 163 105 601.0 0 105 164 111 601.0 0 111 165 133 601.0 0 133 166 111 601.0 0 111 167 142 601.0 0 142 168 143 601.0 0 143 169 111 601.0 0 111 170 108 601.0 0 108 171 123 601.0 0 123 172 95 601.0 0 95 173 115 601.0 0 115 174 133 601.0 0 133 175 135 601.0 0 135 176 107 601.0 0 107 177 130 601.0 0 130 178 124 601.0 0 124 179 123 601.0 0 123 180 104 601.0 0 104 181 134 601.0 0 134 182 149 601.0 0 149 183 140 601.0 0 140 184 142 601.0 0 142 185 177 601.0 0 177 186 155 601.0 0 155 187 143 601.0 0 143 188 145 601.0 0 145 189 129 601.0 0 129 190 134 601.0 0 134 191 143 601.0 0 143 192 146 601.0 0 146 193 124 601.0 0 124 194 133 601.0 0 133 195 189 601.0 0 189 196 131 601.0 0 131 197 114 601.0 0 114 198 114 601.0 0 114 199 102 601.0 0 102 200 111 601.0 0 111 201 134 601.0 0 134 202 138 601.0 0 138 203 125 601.0 0 125 204 200 601.0 0 200 205 120 601.0 0 120 206 213 601.0 0 213 207 221 601.0 0 221 208 175 601.0 0 175 209 212 601.0 0 212 210 153 601.0 0 153 211 113 601.0 0 113 212 119 601.0 0 119 213 134 601.0 0 134 214 132 601.0 0 132 215 122 601.0 0 122 216 80 601.0 0 80 217 147 601.0 0 147 218 143 601.0 0 143 219 115 601.0 0 115 220 155 601.0 0 155 221 178 601.0 0 178 222 228 601.0 0 228 223 181 601.0 0 181 224 243 601.0 0 243 225 125 601.0 0 125 226 121 601.0 0 121 227 143 601.0 0 143 228 137 601.0 0 137 229 151 601.0 0 151 230 152 601.0 0 152 231 143 601.0 0 143 232 172 601.0 0 172 233 179 601.0 0 179 234 231 601.0 0 231 235 1406 601.0 0 1406 236 945 601.0 0 945 237 3361 601.0 0 3361 238 1498 601.0 0 1498 239 1509 601.0 0 1509 240 139 601.0 0 139 241 119 601.0 0 119 242 181 601.0 0 181 243 165 601.0 0 165 244 104 601.0 0 104 245 97 601.0 0 97 246 112 601.0 0 112 247 68 601.0 0 68 248 108 601.0 0 108 249 92 601.0 0 92 250 142 601.0 0 142 251 94 601.0 0 94 252 92 601.0 0 92 253 107 601.0 0 107 254 106 601.0 0 106 255 103 601.0 0 103 256 141 601.0 0 141 257 125 601.0 0 125 258 144 601.0 0 144 259 273 601.0 0 273 260 220 601.0 0 220 261 154 601.0 0 154 262 104 601.0 0 104 263 73 601.0 0 73 264 111 601.0 0 111 265 95 601.0 0 95 266 100 601.0 0 100 267 88 601.0 0 88 268 91 601.0 0 91 269 81 601.0 0 81 270 94 601.0 0 94 271 87 601.0 0 87 272 97 601.0 0 97 273 106 601.0 0 106 274 110 601.0 0 110 275 89 601.0 0 89 276 95 601.0 0 95 277 83 601.0 0 83 278 116 601.0 0 116 279 99 601.0 0 99 280 129 601.0 0 129 281 95 601.0 0 95 282 100 601.0 0 100 283 130 601.0 0 130 284 105 601.0 0 105 285 97 601.0 0 97 286 107 601.0 0 107 287 116 601.0 0 116 288 102 601.0 0 102 289 115 601.0 0 115 290 112 601.0 0 112 291 112 601.0 0 112 292 110 601.0 0 110 293 112 601.0 0 112 294 106 601.0 0 106 295 98 601.0 0 98 296 88 601.0 0 88 297 115 601.0 0 115 298 107 601.0 0 107 299 99 601.0 0 99 300 103 601.0 0 103 301 117 601.0 0 117 Finished in 5.08 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 101,529 Reads with adapters: 37,850 (37.3%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 37,839 (37.3%) Total basepairs processed: 26,949,513 bp Total written (filtered): 10,364,891 bp (38.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 37850 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 13 0.0 3 0 0 11 0 2 19 396 0.0 3 0 386 8 0 2 20 37248 0.0 4 36650 573 19 6 21 122 0.0 4 47 74 1 22 9 0.0 4 0 1 7 1 23 25 0.0 4 1 0 0 23 1 24 1 0.0 4 0 0 0 0 1 29 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 99 14 0.0 4 0 0 0 0 14 104 4 0.0 4 0 0 0 0 4 115 1 0.0 4 1 127 3 0.0 4 0 0 0 0 3 133 1 0.0 4 1 135 1 0.0 4 0 0 0 0 1 139 1 0.0 4 1 153 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 241 1 0.0 4 0 1 244 1 0.0 4 0 0 1 258 2 0.0 4 0 0 0 0 2 260 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.28 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 37,839 Reads with adapters: 795 (2.1%) Reads written (passing filters): 37,839 (100.0%) Total basepairs processed: 10,364,891 bp Total written (filtered): 10,354,966 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 795 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.4% C: 7.8% G: 84.0% T: 2.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 46 591.2 0 46 4 6 147.8 0 6 5 3 37.0 0 3 9 3 0.1 0 2 1 10 9 0.0 1 0 9 11 671 0.0 1 8 663 12 42 0.0 1 2 40 13 3 0.0 1 1 2 15 1 0.0 1 0 1 38 1 0.0 1 1 82 1 0.0 1 1 123 1 0.0 1 1 152 4 0.0 1 0 4 210 3 0.0 1 2 1 211 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.