This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAAGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.29 s (27 us/read; 2.23 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 402,867 (16.4%) Reads that were too short: 11,291 (0.5%) Reads written (passing filters): 391,576 (15.9%) Total basepairs processed: 740,966,583 bp Total written (filtered): 104,668,849 bp (14.1%) === Adapter 1 === Sequence: GAAGCT; Type: regular 5'; Length: 6; Trimmed: 402867 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2400 601.0 0 2400 7 8861 601.0 0 8861 8 157270 601.0 0 157270 9 131206 601.0 0 131206 10 238 601.0 0 238 11 62 601.0 0 62 12 50 601.0 0 50 13 36 601.0 0 36 14 29 601.0 0 29 15 39 601.0 0 39 16 48 601.0 0 48 17 35 601.0 0 35 18 44 601.0 0 44 19 56 601.0 0 56 20 93 601.0 0 93 21 79 601.0 0 79 22 52 601.0 0 52 23 71 601.0 0 71 24 74 601.0 0 74 25 82 601.0 0 82 26 109 601.0 0 109 27 51 601.0 0 51 28 69 601.0 0 69 29 65 601.0 0 65 30 72 601.0 0 72 31 70 601.0 0 70 32 75 601.0 0 75 33 68 601.0 0 68 34 221 601.0 0 221 35 170 601.0 0 170 36 242 601.0 0 242 37 125 601.0 0 125 38 226 601.0 0 226 39 122 601.0 0 122 40 64 601.0 0 64 41 61 601.0 0 61 42 62 601.0 0 62 43 50 601.0 0 50 44 67 601.0 0 67 45 61 601.0 0 61 46 85 601.0 0 85 47 93 601.0 0 93 48 227 601.0 0 227 49 61 601.0 0 61 50 64 601.0 0 64 51 64 601.0 0 64 52 65 601.0 0 65 53 100 601.0 0 100 54 141 601.0 0 141 55 124 601.0 0 124 56 50 601.0 0 50 57 69 601.0 0 69 58 42 601.0 0 42 59 36 601.0 0 36 60 64 601.0 0 64 61 110 601.0 0 110 62 58 601.0 0 58 63 66 601.0 0 66 64 87 601.0 0 87 65 60 601.0 0 60 66 65 601.0 0 65 67 76 601.0 0 76 68 70 601.0 0 70 69 94 601.0 0 94 70 54 601.0 0 54 71 66 601.0 0 66 72 72 601.0 0 72 73 58 601.0 0 58 74 80 601.0 0 80 75 88 601.0 0 88 76 101 601.0 0 101 77 127 601.0 0 127 78 159 601.0 0 159 79 235 601.0 0 235 80 583 601.0 0 583 81 2885 601.0 0 2885 82 3518 601.0 0 3518 83 7246 601.0 0 7246 84 126 601.0 0 126 85 579 601.0 0 579 86 264 601.0 0 264 87 454 601.0 0 454 88 94 601.0 0 94 89 60 601.0 0 60 90 61 601.0 0 61 91 53 601.0 0 53 92 56 601.0 0 56 93 53 601.0 0 53 94 51 601.0 0 51 95 92 601.0 0 92 96 66 601.0 0 66 97 70 601.0 0 70 98 123 601.0 0 123 99 58 601.0 0 58 100 63 601.0 0 63 101 96 601.0 0 96 102 368 601.0 0 368 103 123 601.0 0 123 104 155 601.0 0 155 105 81 601.0 0 81 106 137 601.0 0 137 107 138 601.0 0 138 108 297 601.0 0 297 109 405 601.0 0 405 110 344 601.0 0 344 111 148 601.0 0 148 112 331 601.0 0 331 113 117 601.0 0 117 114 116 601.0 0 116 115 218 601.0 0 218 116 163 601.0 0 163 117 202 601.0 0 202 118 280 601.0 0 280 119 1372 601.0 0 1372 120 870 601.0 0 870 121 1259 601.0 0 1259 122 34179 601.0 0 34179 123 7228 601.0 0 7228 124 6669 601.0 0 6669 125 114 601.0 0 114 126 126 601.0 0 126 127 89 601.0 0 89 128 60 601.0 0 60 129 99 601.0 0 99 130 78 601.0 0 78 131 62 601.0 0 62 132 47 601.0 0 47 133 83 601.0 0 83 134 69 601.0 0 69 135 44 601.0 0 44 136 73 601.0 0 73 137 73 601.0 0 73 138 75 601.0 0 75 139 79 601.0 0 79 140 67 601.0 0 67 141 116 601.0 0 116 142 320 601.0 0 320 143 206 601.0 0 206 144 963 601.0 0 963 145 320 601.0 0 320 146 324 601.0 0 324 147 149 601.0 0 149 148 120 601.0 0 120 149 357 601.0 0 357 150 221 601.0 0 221 151 340 601.0 0 340 152 126 601.0 0 126 153 96 601.0 0 96 154 88 601.0 0 88 155 146 601.0 0 146 156 55 601.0 0 55 157 57 601.0 0 57 158 90 601.0 0 90 159 66 601.0 0 66 160 66 601.0 0 66 161 40 601.0 0 40 162 49 601.0 0 49 163 65 601.0 0 65 164 37 601.0 0 37 165 75 601.0 0 75 166 315 601.0 0 315 167 120 601.0 0 120 168 345 601.0 0 345 169 114 601.0 0 114 170 195 601.0 0 195 171 220 601.0 0 220 172 37 601.0 0 37 173 82 601.0 0 82 174 52 601.0 0 52 175 61 601.0 0 61 176 55 601.0 0 55 177 80 601.0 0 80 178 66 601.0 0 66 179 87 601.0 0 87 180 104 601.0 0 104 181 127 601.0 0 127 182 809 601.0 0 809 183 2160 601.0 0 2160 184 1632 601.0 0 1632 185 73 601.0 0 73 186 107 601.0 0 107 187 121 601.0 0 121 188 42 601.0 0 42 189 37 601.0 0 37 190 55 601.0 0 55 191 32 601.0 0 32 192 40 601.0 0 40 193 91 601.0 0 91 194 119 601.0 0 119 195 123 601.0 0 123 196 93 601.0 0 93 197 40 601.0 0 40 198 103 601.0 0 103 199 95 601.0 0 95 200 179 601.0 0 179 201 98 601.0 0 98 202 35 601.0 0 35 203 30 601.0 0 30 204 34 601.0 0 34 205 128 601.0 0 128 206 69 601.0 0 69 207 57 601.0 0 57 208 43 601.0 0 43 209 66 601.0 0 66 210 64 601.0 0 64 211 56 601.0 0 56 212 50 601.0 0 50 213 55 601.0 0 55 214 45 601.0 0 45 215 35 601.0 0 35 216 34 601.0 0 34 217 33 601.0 0 33 218 47 601.0 0 47 219 79 601.0 0 79 220 57 601.0 0 57 221 60 601.0 0 60 222 52 601.0 0 52 223 50 601.0 0 50 224 46 601.0 0 46 225 44 601.0 0 44 226 105 601.0 0 105 227 57 601.0 0 57 228 69 601.0 0 69 229 60 601.0 0 60 230 61 601.0 0 61 231 61 601.0 0 61 232 50 601.0 0 50 233 50 601.0 0 50 234 56 601.0 0 56 235 36 601.0 0 36 236 56 601.0 0 56 237 51 601.0 0 51 238 55 601.0 0 55 239 70 601.0 0 70 240 226 601.0 0 226 241 163 601.0 0 163 242 191 601.0 0 191 243 67 601.0 0 67 244 71 601.0 0 71 245 63 601.0 0 63 246 47 601.0 0 47 247 64 601.0 0 64 248 50 601.0 0 50 249 53 601.0 0 53 250 54 601.0 0 54 251 34 601.0 0 34 252 39 601.0 0 39 253 65 601.0 0 65 254 59 601.0 0 59 255 96 601.0 0 96 256 73 601.0 0 73 257 58 601.0 0 58 258 115 601.0 0 115 259 101 601.0 0 101 260 205 601.0 0 205 261 235 601.0 0 235 262 111 601.0 0 111 263 149 601.0 0 149 264 88 601.0 0 88 265 77 601.0 0 77 266 684 601.0 0 684 267 359 601.0 0 359 268 749 601.0 0 749 269 149 601.0 0 149 270 60 601.0 0 60 271 55 601.0 0 55 272 72 601.0 0 72 273 62 601.0 0 62 274 373 601.0 0 373 275 263 601.0 0 263 276 225 601.0 0 225 277 121 601.0 0 121 278 122 601.0 0 122 279 148 601.0 0 148 280 94 601.0 0 94 281 199 601.0 0 199 282 63 601.0 0 63 283 84 601.0 0 84 284 44 601.0 0 44 285 128 601.0 0 128 286 230 601.0 0 230 287 261 601.0 0 261 288 318 601.0 0 318 289 192 601.0 0 192 290 154 601.0 0 154 291 67 601.0 0 67 292 121 601.0 0 121 293 165 601.0 0 165 294 227 601.0 0 227 295 164 601.0 0 164 296 220 601.0 0 220 297 89 601.0 0 89 298 95 601.0 0 95 299 92 601.0 0 92 300 100 601.0 0 100 301 82 601.0 0 82 Finished in 16.28 s (42 us/read; 1.44 M reads/minute). === Summary === Total reads processed: 391,576 Reads with adapters: 163,053 (41.6%) Reads that were too short: 1,002 (0.3%) Reads written (passing filters): 162,051 (41.4%) Total basepairs processed: 104,668,849 bp Total written (filtered): 44,417,159 bp (42.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 163053 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 36 0.0 2 0 0 0 36 15 118 0.0 3 0 0 74 44 16 1199 0.0 3 0 973 127 99 17 157345 0.0 3 145868 9352 1712 413 18 501 0.0 3 15 369 52 65 19 464 0.0 3 3 0 273 188 20 275 0.0 3 5 0 0 270 22 50 0.0 3 1 0 0 49 24 1 0.0 3 0 0 0 1 25 45 0.0 3 0 0 27 18 26 2 0.0 3 1 0 0 1 27 2 0.0 3 0 0 0 2 28 58 0.0 3 1 0 0 57 29 40 0.0 3 0 0 0 40 32 2 0.0 3 0 0 0 2 34 533 0.0 3 0 0 0 533 35 1 0.0 3 1 36 1 0.0 3 0 0 0 1 37 3 0.0 3 0 0 0 3 38 7 0.0 3 0 0 0 7 39 1 0.0 3 0 0 0 1 40 2 0.0 3 0 0 0 2 41 1 0.0 3 0 0 0 1 44 5 0.0 3 0 0 1 4 45 1 0.0 3 0 0 0 1 46 4 0.0 3 0 0 0 4 47 16 0.0 3 0 0 0 16 48 11 0.0 3 0 0 0 11 49 126 0.0 3 0 0 3 123 50 912 0.0 3 0 0 10 902 51 38 0.0 3 0 0 1 37 52 125 0.0 3 0 0 3 122 53 93 0.0 3 0 0 4 89 54 5 0.0 3 0 0 0 5 55 9 0.0 3 0 0 0 9 56 2 0.0 3 0 0 0 2 58 2 0.0 3 0 0 0 2 59 4 0.0 3 0 0 0 4 60 4 0.0 3 0 0 0 4 61 3 0.0 3 0 0 0 3 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 6 0.0 3 0 0 0 6 67 6 0.0 3 1 0 1 4 68 6 0.0 3 0 0 0 6 69 6 0.0 3 0 0 0 6 70 6 0.0 3 0 0 0 6 71 3 0.0 3 0 0 0 3 72 6 0.0 3 0 0 0 6 73 14 0.0 3 0 0 2 12 74 1 0.0 3 0 0 0 1 75 5 0.0 3 0 0 0 5 76 28 0.0 3 0 0 1 27 77 3 0.0 3 0 0 0 3 78 6 0.0 3 0 0 0 6 81 2 0.0 3 0 0 0 2 82 2 0.0 3 0 0 1 1 83 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 88 3 0.0 3 1 0 0 2 89 4 0.0 3 0 0 0 4 90 3 0.0 3 0 0 0 3 91 1 0.0 3 0 0 1 92 1 0.0 3 1 93 3 0.0 3 0 0 0 3 95 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 1 98 7 0.0 3 0 0 0 7 99 1 0.0 3 0 0 0 1 100 4 0.0 3 0 0 0 4 101 55 0.0 3 0 0 1 54 102 12 0.0 3 0 0 1 11 103 4 0.0 3 0 0 0 4 104 1 0.0 3 0 0 0 1 105 3 0.0 3 0 0 0 3 106 21 0.0 3 0 0 1 20 107 8 0.0 3 0 0 0 8 108 1 0.0 3 0 0 0 1 109 5 0.0 3 0 0 0 5 110 19 0.0 3 0 0 0 19 111 1 0.0 3 0 0 0 1 112 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 118 4 0.0 3 0 0 0 4 119 1 0.0 3 0 0 1 120 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 124 2 0.0 3 0 0 0 2 126 2 0.0 3 0 0 0 2 127 6 0.0 3 0 1 0 5 130 4 0.0 3 0 0 1 3 132 2 0.0 3 0 0 0 2 133 3 0.0 3 0 0 0 3 134 2 0.0 3 0 0 0 2 135 1 0.0 3 0 0 0 1 136 3 0.0 3 0 0 0 3 137 2 0.0 3 0 0 0 2 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 3 0.0 3 0 0 1 2 142 3 0.0 3 0 0 0 3 147 2 0.0 3 0 0 0 2 149 1 0.0 3 0 0 0 1 150 1 0.0 3 0 0 0 1 151 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 153 2 0.0 3 0 0 0 2 154 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 156 7 0.0 3 0 0 0 7 157 9 0.0 3 0 0 0 9 158 1 0.0 3 0 0 0 1 160 4 0.0 3 0 0 0 4 161 16 0.0 3 1 0 0 15 162 126 0.0 3 0 0 4 122 163 35 0.0 3 0 0 0 35 164 50 0.0 3 0 0 2 48 165 141 0.0 3 0 0 7 134 166 16 0.0 3 0 0 0 16 167 14 0.0 3 0 0 2 12 170 2 0.0 3 0 0 0 2 172 2 0.0 3 0 0 0 2 173 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 176 3 0.0 3 0 0 1 2 178 3 0.0 3 0 0 0 3 179 8 0.0 3 0 0 1 7 181 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 7 0.0 3 0 0 0 7 184 6 0.0 3 0 0 0 6 185 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 187 3 0.0 3 0 0 0 3 188 2 0.0 3 0 0 0 2 189 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 9 0.0 3 0 0 0 9 193 1 0.0 3 0 0 0 1 194 9 0.0 3 0 0 0 9 197 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 205 28 0.0 3 0 0 0 28 206 3 0.0 3 0 0 0 3 207 2 0.0 3 0 0 0 2 208 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 217 6 0.0 3 0 0 0 6 218 1 0.0 3 0 0 0 1 221 3 0.0 3 0 0 0 3 225 2 0.0 3 0 0 0 2 226 2 0.0 3 0 0 0 2 236 2 0.0 3 0 0 0 2 237 2 0.0 3 0 0 0 2 238 1 0.0 3 0 0 0 1 244 2 0.0 3 0 0 0 2 255 1 0.0 3 0 0 0 1 265 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 273 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 22 0.0 3 0 0 0 22 280 21 0.0 3 0 0 1 20 281 35 0.0 3 0 0 0 35 282 29 0.0 3 0 0 1 28 283 8 0.0 3 0 0 2 6 285 3 0.0 3 0 0 1 2 286 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 288 5 0.0 3 0 0 0 5 291 1 0.0 3 0 0 0 1 292 3 0.0 3 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.65 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 162,051 Reads with adapters: 2,196 (1.4%) Reads written (passing filters): 162,051 (100.0%) Total basepairs processed: 44,417,159 bp Total written (filtered): 44,410,203 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 2196 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 59.3% C: 14.4% G: 22.3% T: 4.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1855 2532.0 0 1855 4 316 633.0 0 316 5 23 158.3 0 23 6 2 39.6 0 2