This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAAGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.77 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 432,774 (17.6%) Reads that were too short: 10,007 (0.4%) Reads written (passing filters): 422,767 (17.2%) Total basepairs processed: 740,966,583 bp Total written (filtered): 113,163,400 bp (15.3%) === Adapter 1 === Sequence: GAAGCT; Type: regular 5'; Length: 6; Trimmed: 432774 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1815 601.0 0 1815 7 5782 601.0 0 5782 8 145921 601.0 0 145921 9 171255 601.0 0 171255 10 208 601.0 0 208 11 56 601.0 0 56 12 42 601.0 0 42 13 58 601.0 0 58 14 47 601.0 0 47 15 45 601.0 0 45 16 69 601.0 0 69 17 49 601.0 0 49 18 59 601.0 0 59 19 45 601.0 0 45 20 39 601.0 0 39 21 65 601.0 0 65 22 57 601.0 0 57 23 43 601.0 0 43 24 56 601.0 0 56 25 54 601.0 0 54 26 71 601.0 0 71 27 60 601.0 0 60 28 59 601.0 0 59 29 64 601.0 0 64 30 100 601.0 0 100 31 85 601.0 0 85 32 75 601.0 0 75 33 67 601.0 0 67 34 109 601.0 0 109 35 58 601.0 0 58 36 250 601.0 0 250 37 162 601.0 0 162 38 250 601.0 0 250 39 121 601.0 0 121 40 80 601.0 0 80 41 70 601.0 0 70 42 74 601.0 0 74 43 55 601.0 0 55 44 62 601.0 0 62 45 78 601.0 0 78 46 108 601.0 0 108 47 87 601.0 0 87 48 232 601.0 0 232 49 59 601.0 0 59 50 55 601.0 0 55 51 81 601.0 0 81 52 79 601.0 0 79 53 105 601.0 0 105 54 136 601.0 0 136 55 103 601.0 0 103 56 61 601.0 0 61 57 69 601.0 0 69 58 56 601.0 0 56 59 58 601.0 0 58 60 86 601.0 0 86 61 106 601.0 0 106 62 76 601.0 0 76 63 74 601.0 0 74 64 130 601.0 0 130 65 66 601.0 0 66 66 76 601.0 0 76 67 83 601.0 0 83 68 80 601.0 0 80 69 117 601.0 0 117 70 79 601.0 0 79 71 82 601.0 0 82 72 98 601.0 0 98 73 97 601.0 0 97 74 107 601.0 0 107 75 79 601.0 0 79 76 127 601.0 0 127 77 159 601.0 0 159 78 152 601.0 0 152 79 237 601.0 0 237 80 585 601.0 0 585 81 3664 601.0 0 3664 82 4400 601.0 0 4400 83 9532 601.0 0 9532 84 125 601.0 0 125 85 246 601.0 0 246 86 152 601.0 0 152 87 191 601.0 0 191 88 112 601.0 0 112 89 68 601.0 0 68 90 80 601.0 0 80 91 54 601.0 0 54 92 81 601.0 0 81 93 50 601.0 0 50 94 59 601.0 0 59 95 93 601.0 0 93 96 92 601.0 0 92 97 70 601.0 0 70 98 148 601.0 0 148 99 108 601.0 0 108 100 86 601.0 0 86 101 87 601.0 0 87 102 158 601.0 0 158 103 95 601.0 0 95 104 126 601.0 0 126 105 96 601.0 0 96 106 115 601.0 0 115 107 195 601.0 0 195 108 456 601.0 0 456 109 414 601.0 0 414 110 364 601.0 0 364 111 130 601.0 0 130 112 354 601.0 0 354 113 147 601.0 0 147 114 129 601.0 0 129 115 202 601.0 0 202 116 242 601.0 0 242 117 211 601.0 0 211 118 238 601.0 0 238 119 650 601.0 0 650 120 592 601.0 0 592 121 911 601.0 0 911 122 37696 601.0 0 37696 123 6800 601.0 0 6800 124 7169 601.0 0 7169 125 95 601.0 0 95 126 98 601.0 0 98 127 97 601.0 0 97 128 59 601.0 0 59 129 113 601.0 0 113 130 84 601.0 0 84 131 75 601.0 0 75 132 46 601.0 0 46 133 64 601.0 0 64 134 78 601.0 0 78 135 62 601.0 0 62 136 91 601.0 0 91 137 72 601.0 0 72 138 67 601.0 0 67 139 68 601.0 0 68 140 68 601.0 0 68 141 96 601.0 0 96 142 371 601.0 0 371 143 222 601.0 0 222 144 928 601.0 0 928 145 261 601.0 0 261 146 237 601.0 0 237 147 100 601.0 0 100 148 114 601.0 0 114 149 162 601.0 0 162 150 128 601.0 0 128 151 226 601.0 0 226 152 115 601.0 0 115 153 67 601.0 0 67 154 62 601.0 0 62 155 70 601.0 0 70 156 45 601.0 0 45 157 67 601.0 0 67 158 69 601.0 0 69 159 48 601.0 0 48 160 64 601.0 0 64 161 71 601.0 0 71 162 49 601.0 0 49 163 103 601.0 0 103 164 55 601.0 0 55 165 100 601.0 0 100 166 361 601.0 0 361 167 130 601.0 0 130 168 410 601.0 0 410 169 110 601.0 0 110 170 186 601.0 0 186 171 189 601.0 0 189 172 67 601.0 0 67 173 62 601.0 0 62 174 42 601.0 0 42 175 72 601.0 0 72 176 77 601.0 0 77 177 96 601.0 0 96 178 80 601.0 0 80 179 96 601.0 0 96 180 112 601.0 0 112 181 125 601.0 0 125 182 904 601.0 0 904 183 2706 601.0 0 2706 184 2059 601.0 0 2059 185 75 601.0 0 75 186 87 601.0 0 87 187 72 601.0 0 72 188 59 601.0 0 59 189 48 601.0 0 48 190 52 601.0 0 52 191 57 601.0 0 57 192 63 601.0 0 63 193 60 601.0 0 60 194 61 601.0 0 61 195 84 601.0 0 84 196 57 601.0 0 57 197 44 601.0 0 44 198 80 601.0 0 80 199 63 601.0 0 63 200 107 601.0 0 107 201 59 601.0 0 59 202 52 601.0 0 52 203 59 601.0 0 59 204 50 601.0 0 50 205 95 601.0 0 95 206 65 601.0 0 65 207 56 601.0 0 56 208 52 601.0 0 52 209 57 601.0 0 57 210 59 601.0 0 59 211 46 601.0 0 46 212 67 601.0 0 67 213 50 601.0 0 50 214 76 601.0 0 76 215 57 601.0 0 57 216 44 601.0 0 44 217 51 601.0 0 51 218 57 601.0 0 57 219 65 601.0 0 65 220 73 601.0 0 73 221 73 601.0 0 73 222 62 601.0 0 62 223 64 601.0 0 64 224 65 601.0 0 65 225 72 601.0 0 72 226 74 601.0 0 74 227 45 601.0 0 45 228 55 601.0 0 55 229 61 601.0 0 61 230 73 601.0 0 73 231 44 601.0 0 44 232 38 601.0 0 38 233 59 601.0 0 59 234 45 601.0 0 45 235 50 601.0 0 50 236 58 601.0 0 58 237 88 601.0 0 88 238 54 601.0 0 54 239 80 601.0 0 80 240 155 601.0 0 155 241 134 601.0 0 134 242 128 601.0 0 128 243 61 601.0 0 61 244 59 601.0 0 59 245 69 601.0 0 69 246 46 601.0 0 46 247 65 601.0 0 65 248 51 601.0 0 51 249 60 601.0 0 60 250 64 601.0 0 64 251 52 601.0 0 52 252 57 601.0 0 57 253 57 601.0 0 57 254 67 601.0 0 67 255 90 601.0 0 90 256 70 601.0 0 70 257 57 601.0 0 57 258 83 601.0 0 83 259 103 601.0 0 103 260 208 601.0 0 208 261 168 601.0 0 168 262 82 601.0 0 82 263 140 601.0 0 140 264 120 601.0 0 120 265 106 601.0 0 106 266 542 601.0 0 542 267 256 601.0 0 256 268 750 601.0 0 750 269 107 601.0 0 107 270 64 601.0 0 64 271 47 601.0 0 47 272 63 601.0 0 63 273 65 601.0 0 65 274 290 601.0 0 290 275 157 601.0 0 157 276 120 601.0 0 120 277 83 601.0 0 83 278 72 601.0 0 72 279 92 601.0 0 92 280 58 601.0 0 58 281 120 601.0 0 120 282 76 601.0 0 76 283 89 601.0 0 89 284 51 601.0 0 51 285 110 601.0 0 110 286 223 601.0 0 223 287 230 601.0 0 230 288 219 601.0 0 219 289 176 601.0 0 176 290 72 601.0 0 72 291 61 601.0 0 61 292 108 601.0 0 108 293 119 601.0 0 119 294 161 601.0 0 161 295 133 601.0 0 133 296 250 601.0 0 250 297 94 601.0 0 94 298 69 601.0 0 69 299 76 601.0 0 76 300 90 601.0 0 90 301 71 601.0 0 71 Finished in 19.01 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 422,767 Reads with adapters: 178,630 (42.3%) Reads that were too short: 48 (0.0%) Reads written (passing filters): 178,582 (42.2%) Total basepairs processed: 113,163,400 bp Total written (filtered): 48,560,568 bp (42.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 178630 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 75 0.0 3 0 0 72 2 1 19 2537 0.0 3 0 2498 31 5 3 20 172442 0.0 4 169689 2653 90 9 1 21 373 0.0 4 51 313 8 0 1 22 2810 0.0 4 2753 43 13 1 23 119 0.0 4 2 2 1 99 15 24 8 0.0 4 1 0 1 0 6 25 4 0.0 4 0 1 0 3 26 2 0.0 4 1 0 0 0 1 27 1 0.0 4 1 28 2 0.0 4 1 0 1 33 1 0.0 4 0 0 0 0 1 42 1 0.0 4 1 46 1 0.0 4 1 47 1 0.0 4 0 0 1 49 1 0.0 4 0 0 0 0 1 74 1 0.0 4 1 92 4 0.0 4 1 1 1 0 1 93 1 0.0 4 0 0 0 0 1 94 2 0.0 4 1 1 97 1 0.0 4 1 98 2 0.0 4 1 0 1 99 2 0.0 4 1 0 0 0 1 100 1 0.0 4 0 0 0 0 1 106 1 0.0 4 1 126 2 0.0 4 0 0 0 0 2 127 190 0.0 4 0 0 0 0 190 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 1 144 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 149 2 0.0 4 0 0 0 0 2 150 3 0.0 4 0 0 0 0 3 153 3 0.0 4 0 0 0 0 3 168 1 0.0 4 0 0 0 0 1 178 2 0.0 4 0 0 0 0 2 179 4 0.0 4 0 0 0 0 4 202 1 0.0 4 0 0 0 1 207 1 0.0 4 0 0 0 0 1 210 1 0.0 4 1 227 2 0.0 4 0 0 0 0 2 228 2 0.0 4 0 0 0 0 2 230 1 0.0 4 0 0 0 0 1 232 3 0.0 4 0 0 0 0 3 234 1 0.0 4 0 0 0 0 1 235 2 0.0 4 0 0 0 0 2 242 2 0.0 4 0 0 0 0 2 252 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 262 5 0.0 4 0 0 0 0 5 263 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 6.42 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 178,582 Reads with adapters: 32,912 (18.4%) Reads written (passing filters): 178,582 (100.0%) Total basepairs processed: 48,560,568 bp Total written (filtered): 48,242,717 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 32912 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.7% C: 2.4% G: 95.8% T: 1.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 45 2790.3 0 45 4 25 697.6 0 25 5 10 174.4 0 10 6 10 43.6 0 10 7 10 10.9 0 10 8 3 2.7 0 3 9 19948 0.7 0 611 19337 10 11254 0.2 1 378 10876 11 1514 0.0 1 11 1503 12 36 0.0 1 1 35 13 8 0.0 1 0 8 14 1 0.0 1 0 1 20 1 0.0 1 1 22 1 0.0 1 1 39 1 0.0 1 1 64 1 0.0 1 1 91 1 0.0 1 1 105 1 0.0 1 1 120 3 0.0 1 3 125 1 0.0 1 1 138 2 0.0 1 2 141 1 0.0 1 1 150 6 0.0 1 0 6 161 1 0.0 1 1 169 2 0.0 1 1 1 170 1 0.0 1 1 189 2 0.0 1 0 2 195 1 0.0 1 1 208 17 0.0 1 12 5 228 2 0.0 1 2 245 1 0.0 1 1 257 2 0.0 1 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.