This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 63.13 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,461,683 Reads with adapters: 490,024 (19.9%) Reads that were too short: 357,809 (14.5%) Reads written (passing filters): 132,215 (5.4%) Total basepairs processed: 740,966,583 bp Total written (filtered): 26,645,891 bp (3.6%) === Adapter 1 === Sequence: GTCCGG; Type: regular 5'; Length: 6; Trimmed: 490024 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2685 601.0 0 2685 7 20506 601.0 0 20506 8 1308 601.0 0 1308 9 33336 601.0 0 33336 10 109 601.0 0 109 11 50 601.0 0 50 12 47 601.0 0 47 13 44 601.0 0 44 14 45 601.0 0 45 15 35 601.0 0 35 16 54 601.0 0 54 17 43 601.0 0 43 18 47 601.0 0 47 19 39 601.0 0 39 20 34 601.0 0 34 21 35 601.0 0 35 22 36 601.0 0 36 23 41 601.0 0 41 24 35 601.0 0 35 25 28 601.0 0 28 26 21 601.0 0 21 27 31 601.0 0 31 28 20 601.0 0 20 29 26 601.0 0 26 30 37 601.0 0 37 31 145 601.0 0 145 32 109 601.0 0 109 33 39 601.0 0 39 34 58 601.0 0 58 35 83 601.0 0 83 36 42 601.0 0 42 37 39 601.0 0 39 38 56 601.0 0 56 39 47 601.0 0 47 40 25 601.0 0 25 41 40 601.0 0 40 42 42 601.0 0 42 43 32 601.0 0 32 44 33 601.0 0 33 45 33 601.0 0 33 46 32 601.0 0 32 47 42 601.0 0 42 48 43 601.0 0 43 49 30 601.0 0 30 50 30 601.0 0 30 51 26 601.0 0 26 52 32 601.0 0 32 53 38 601.0 0 38 54 29 601.0 0 29 55 38 601.0 0 38 56 50 601.0 0 50 57 60 601.0 0 60 58 54 601.0 0 54 59 68 601.0 0 68 60 31 601.0 0 31 61 50 601.0 0 50 62 29 601.0 0 29 63 42 601.0 0 42 64 23 601.0 0 23 65 67 601.0 0 67 66 113 601.0 0 113 67 90 601.0 0 90 68 27 601.0 0 27 69 40 601.0 0 40 70 60 601.0 0 60 71 47 601.0 0 47 72 56 601.0 0 56 73 55 601.0 0 55 74 47 601.0 0 47 75 258 601.0 0 258 76 301 601.0 0 301 77 277 601.0 0 277 78 73 601.0 0 73 79 26 601.0 0 26 80 33 601.0 0 33 81 42 601.0 0 42 82 37 601.0 0 37 83 33 601.0 0 33 84 46 601.0 0 46 85 34 601.0 0 34 86 33 601.0 0 33 87 35 601.0 0 35 88 54 601.0 0 54 89 53 601.0 0 53 90 51 601.0 0 51 91 94 601.0 0 94 92 87 601.0 0 87 93 85 601.0 0 85 94 58 601.0 0 58 95 46 601.0 0 46 96 42 601.0 0 42 97 46 601.0 0 46 98 74 601.0 0 74 99 90 601.0 0 90 100 98 601.0 0 98 101 48 601.0 0 48 102 55 601.0 0 55 103 79 601.0 0 79 104 47 601.0 0 47 105 53 601.0 0 53 106 53 601.0 0 53 107 53 601.0 0 53 108 57 601.0 0 57 109 41 601.0 0 41 110 66 601.0 0 66 111 79 601.0 0 79 112 112 601.0 0 112 113 2253 601.0 0 2253 114 1355 601.0 0 1355 115 1254 601.0 0 1254 116 95 601.0 0 95 117 41 601.0 0 41 118 42 601.0 0 42 119 58 601.0 0 58 120 146 601.0 0 146 121 81 601.0 0 81 122 85 601.0 0 85 123 91 601.0 0 91 124 83 601.0 0 83 125 76 601.0 0 76 126 63 601.0 0 63 127 82 601.0 0 82 128 84 601.0 0 84 129 70 601.0 0 70 130 71 601.0 0 71 131 142 601.0 0 142 132 80 601.0 0 80 133 77 601.0 0 77 134 132 601.0 0 132 135 63 601.0 0 63 136 80 601.0 0 80 137 69 601.0 0 69 138 58 601.0 0 58 139 90 601.0 0 90 140 88 601.0 0 88 141 81 601.0 0 81 142 106 601.0 0 106 143 120 601.0 0 120 144 154 601.0 0 154 145 219 601.0 0 219 146 265 601.0 0 265 147 5878 601.0 0 5878 148 4245 601.0 0 4245 149 1240 601.0 0 1240 150 667 601.0 0 667 151 41 601.0 0 41 152 68 601.0 0 68 153 63 601.0 0 63 154 83 601.0 0 83 155 51 601.0 0 51 156 89 601.0 0 89 157 75 601.0 0 75 158 81 601.0 0 81 159 64 601.0 0 64 160 71 601.0 0 71 161 63 601.0 0 63 162 72 601.0 0 72 163 68 601.0 0 68 164 63 601.0 0 63 165 48 601.0 0 48 166 38 601.0 0 38 167 56 601.0 0 56 168 88 601.0 0 88 169 160 601.0 0 160 170 89 601.0 0 89 171 58 601.0 0 58 172 79 601.0 0 79 173 100 601.0 0 100 174 113 601.0 0 113 175 78 601.0 0 78 176 82 601.0 0 82 177 63 601.0 0 63 178 102 601.0 0 102 179 153 601.0 0 153 180 167 601.0 0 167 181 209 601.0 0 209 182 177 601.0 0 177 183 144 601.0 0 144 184 131 601.0 0 131 185 217 601.0 0 217 186 303 601.0 0 303 187 438 601.0 0 438 188 311 601.0 0 311 189 788 601.0 0 788 190 1158 601.0 0 1158 191 1219 601.0 0 1219 192 904 601.0 0 904 193 706 601.0 0 706 194 590 601.0 0 590 195 748 601.0 0 748 196 600 601.0 0 600 197 705 601.0 0 705 198 934 601.0 0 934 199 4108 601.0 0 4108 200 21085 601.0 0 21085 201 10717 601.0 0 10717 202 4773 601.0 0 4773 203 2163 601.0 0 2163 204 2933 601.0 0 2933 205 44135 601.0 0 44135 206 66228 601.0 0 66228 207 34619 601.0 0 34619 208 11395 601.0 0 11395 209 6343 601.0 0 6343 210 3252 601.0 0 3252 211 8808 601.0 0 8808 212 3015 601.0 0 3015 213 1905 601.0 0 1905 214 602 601.0 0 602 215 520 601.0 0 520 216 640 601.0 0 640 217 699 601.0 0 699 218 859 601.0 0 859 219 1198 601.0 0 1198 220 1626 601.0 0 1626 221 1856 601.0 0 1856 222 1987 601.0 0 1987 223 882 601.0 0 882 224 1723 601.0 0 1723 225 16227 601.0 0 16227 226 32625 601.0 0 32625 227 15819 601.0 0 15819 228 4182 601.0 0 4182 229 70 601.0 0 70 230 72 601.0 0 72 231 68 601.0 0 68 232 39 601.0 0 39 233 43 601.0 0 43 234 75 601.0 0 75 235 97 601.0 0 97 236 106 601.0 0 106 237 88 601.0 0 88 238 126 601.0 0 126 239 72 601.0 0 72 240 128 601.0 0 128 241 69 601.0 0 69 242 114 601.0 0 114 243 76 601.0 0 76 244 691 601.0 0 691 245 778 601.0 0 778 246 687 601.0 0 687 247 230 601.0 0 230 248 366 601.0 0 366 249 815 601.0 0 815 250 337 601.0 0 337 251 330 601.0 0 330 252 296 601.0 0 296 253 422 601.0 0 422 254 200 601.0 0 200 255 442 601.0 0 442 256 263 601.0 0 263 257 373 601.0 0 373 258 387 601.0 0 387 259 1046 601.0 0 1046 260 1680 601.0 0 1680 261 2476 601.0 0 2476 262 45977 601.0 0 45977 263 12820 601.0 0 12820 264 5494 601.0 0 5494 265 260 601.0 0 260 266 195 601.0 0 195 267 51 601.0 0 51 268 122 601.0 0 122 269 189 601.0 0 189 270 195 601.0 0 195 271 217 601.0 0 217 272 61 601.0 0 61 273 58 601.0 0 58 274 121 601.0 0 121 275 139 601.0 0 139 276 276 601.0 0 276 277 110 601.0 0 110 278 92 601.0 0 92 279 60 601.0 0 60 280 83 601.0 0 83 281 100 601.0 0 100 282 146 601.0 0 146 283 231 601.0 0 231 284 220 601.0 0 220 285 174 601.0 0 174 286 65 601.0 0 65 287 81 601.0 0 81 288 90 601.0 0 90 289 135 601.0 0 135 290 150 601.0 0 150 291 128 601.0 0 128 292 2420 601.0 0 2420 293 939 601.0 0 939 294 534 601.0 0 534 295 187 601.0 0 187 296 319 601.0 0 319 297 274 601.0 0 274 298 244 601.0 0 244 299 137 601.0 0 137 300 373 601.0 0 373 301 266 601.0 0 266 Finished in 5.02 s (38 us/read; 1.58 M reads/minute). === Summary === Total reads processed: 132,215 Reads with adapters: 35,597 (26.9%) Reads that were too short: 378 (0.3%) Reads written (passing filters): 35,219 (26.6%) Total basepairs processed: 26,645,891 bp Total written (filtered): 9,653,623 bp (36.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 35597 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 30 0.0 3 0 0 19 11 16 358 0.0 3 0 292 49 17 17 34204 0.0 3 31489 2184 407 124 18 209 0.0 3 68 85 20 36 19 78 0.0 3 0 1 41 36 20 148 0.0 3 4 0 2 142 21 7 0.0 3 0 0 1 6 23 3 0.0 3 1 1 0 1 24 1 0.0 3 1 26 65 0.0 3 0 0 2 63 27 3 0.0 3 0 1 0 2 28 1 0.0 3 0 0 0 1 29 2 0.0 3 0 0 0 2 30 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 0 2 33 2 0.0 3 0 0 0 2 34 3 0.0 3 0 0 0 3 35 2 0.0 3 0 0 0 2 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 39 2 0.0 3 0 0 0 2 41 2 0.0 3 0 0 0 2 43 2 0.0 3 0 0 0 2 44 3 0.0 3 0 0 0 3 46 1 0.0 3 0 0 0 1 47 4 0.0 3 0 0 0 4 48 3 0.0 3 0 0 0 3 49 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 53 4 0.0 3 0 0 0 4 54 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 58 36 0.0 3 0 0 1 35 61 5 0.0 3 0 0 1 4 62 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 65 5 0.0 3 0 0 0 5 66 3 0.0 3 0 0 0 3 67 2 0.0 3 0 0 0 2 68 10 0.0 3 0 0 1 9 69 4 0.0 3 0 0 0 4 70 1 0.0 3 0 0 0 1 71 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 74 1 0.0 3 0 0 0 1 75 52 0.0 3 0 0 1 51 77 3 0.0 3 0 0 0 3 78 90 0.0 3 0 0 2 88 79 8 0.0 3 0 0 0 8 80 6 0.0 3 0 0 1 5 81 28 0.0 3 0 0 0 28 82 3 0.0 3 0 0 0 3 83 2 0.0 3 0 0 0 2 86 1 0.0 3 0 0 0 1 87 4 0.0 3 0 0 0 4 91 4 0.0 3 0 0 0 4 94 1 0.0 3 0 0 0 1 95 3 0.0 3 0 0 0 3 96 3 0.0 3 0 0 0 3 97 3 0.0 3 0 0 0 3 98 6 0.0 3 0 0 1 5 100 2 0.0 3 0 0 1 1 101 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 104 3 0.0 3 0 0 0 3 105 3 0.0 3 0 1 0 2 106 5 0.0 3 0 0 0 5 107 3 0.0 3 0 0 0 3 109 3 0.0 3 0 0 0 3 110 2 0.0 3 0 0 0 2 111 1 0.0 3 0 0 0 1 112 6 0.0 3 0 0 0 6 113 5 0.0 3 0 0 1 4 115 1 0.0 3 0 0 0 1 116 3 0.0 3 0 0 0 3 117 1 0.0 3 0 0 0 1 119 3 0.0 3 0 0 0 3 124 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 129 5 0.0 3 0 0 0 5 132 2 0.0 3 0 0 1 1 136 1 0.0 3 0 0 0 1 138 4 0.0 3 0 0 0 4 142 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 151 4 0.0 3 0 0 0 4 155 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 170 8 0.0 3 0 0 0 8 172 4 0.0 3 0 0 1 3 173 6 0.0 3 1 0 0 5 175 1 0.0 3 0 0 0 1 179 3 0.0 3 0 0 0 3 180 9 0.0 3 0 0 1 8 182 1 0.0 3 0 0 0 1 183 3 0.0 3 0 0 0 3 185 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 194 3 0.0 3 0 0 1 2 205 2 0.0 3 0 0 0 2 210 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 221 6 0.0 3 0 0 0 6 222 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 227 2 0.0 3 0 0 0 2 228 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 237 1 0.0 3 0 0 0 1 249 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 276 1 0.0 3 0 0 1 279 4 0.0 3 0 0 0 4 280 3 0.0 3 0 0 0 3 281 8 0.0 3 0 0 0 8 282 2 0.0 3 0 0 0 2 283 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 293 1 0.0 3 0 0 0 1 294 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.24 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 35,219 Reads with adapters: 593 (1.7%) Reads written (passing filters): 35,219 (100.0%) Total basepairs processed: 9,653,623 bp Total written (filtered): 9,651,791 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 593 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 44.4% C: 13.5% G: 38.6% T: 3.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 562 550.3 0 562 4 23 137.6 0 23 5 2 34.4 0 2 6 3 8.6 0 3 7 1 2.1 0 1 9 1 0.1 0 0 1 10 1 0.0 1 0 1