This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.30 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 135,769 (5.3%) Reads that were too short: 58,064 (2.3%) Reads written (passing filters): 77,705 (3.1%) Total basepairs processed: 766,381,518 bp Total written (filtered): 19,026,838 bp (2.5%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 135769 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 535 621.6 0 535 7 2492 621.6 0 2492 8 23550 621.6 0 23550 9 20375 621.6 0 20375 10 59 621.6 0 59 11 27 621.6 0 27 12 49 621.6 0 49 13 31 621.6 0 31 14 36 621.6 0 36 15 28 621.6 0 28 16 25 621.6 0 25 17 56 621.6 0 56 18 29 621.6 0 29 19 29 621.6 0 29 20 31 621.6 0 31 21 23 621.6 0 23 22 30 621.6 0 30 23 28 621.6 0 28 24 52 621.6 0 52 25 40 621.6 0 40 26 37 621.6 0 37 27 26 621.6 0 26 28 29 621.6 0 29 29 34 621.6 0 34 30 29 621.6 0 29 31 32 621.6 0 32 32 76 621.6 0 76 33 42 621.6 0 42 34 61 621.6 0 61 35 74 621.6 0 74 36 44 621.6 0 44 37 266 621.6 0 266 38 113 621.6 0 113 39 182 621.6 0 182 40 80 621.6 0 80 41 81 621.6 0 81 42 172 621.6 0 172 43 31 621.6 0 31 44 43 621.6 0 43 45 29 621.6 0 29 46 31 621.6 0 31 47 65 621.6 0 65 48 47 621.6 0 47 49 54 621.6 0 54 50 37 621.6 0 37 51 34 621.6 0 34 52 331 621.6 0 331 53 185 621.6 0 185 54 367 621.6 0 367 55 34 621.6 0 34 56 32 621.6 0 32 57 52 621.6 0 52 58 31 621.6 0 31 59 39 621.6 0 39 60 43 621.6 0 43 61 69 621.6 0 69 62 59 621.6 0 59 63 32 621.6 0 32 64 25 621.6 0 25 65 64 621.6 0 64 66 90 621.6 0 90 67 52 621.6 0 52 68 102 621.6 0 102 69 16 621.6 0 16 70 41 621.6 0 41 71 55 621.6 0 55 72 103 621.6 0 103 73 86 621.6 0 86 74 24 621.6 0 24 75 29 621.6 0 29 76 24 621.6 0 24 77 36 621.6 0 36 78 22 621.6 0 22 79 27 621.6 0 27 80 25 621.6 0 25 81 60 621.6 0 60 82 21 621.6 0 21 83 58 621.6 0 58 84 155 621.6 0 155 85 85 621.6 0 85 86 153 621.6 0 153 87 45 621.6 0 45 88 34 621.6 0 34 89 41 621.6 0 41 90 108 621.6 0 108 91 93 621.6 0 93 92 97 621.6 0 97 93 130 621.6 0 130 94 77 621.6 0 77 95 141 621.6 0 141 96 59 621.6 0 59 97 94 621.6 0 94 98 53 621.6 0 53 99 31 621.6 0 31 100 27 621.6 0 27 101 28 621.6 0 28 102 25 621.6 0 25 103 38 621.6 0 38 104 109 621.6 0 109 105 102 621.6 0 102 106 158 621.6 0 158 107 128 621.6 0 128 108 374 621.6 0 374 109 271 621.6 0 271 110 589 621.6 0 589 111 164 621.6 0 164 112 189 621.6 0 189 113 192 621.6 0 192 114 91 621.6 0 91 115 70 621.6 0 70 116 50 621.6 0 50 117 92 621.6 0 92 118 70 621.6 0 70 119 158 621.6 0 158 120 41 621.6 0 41 121 32 621.6 0 32 122 56 621.6 0 56 123 45 621.6 0 45 124 50 621.6 0 50 125 60 621.6 0 60 126 105 621.6 0 105 127 474 621.6 0 474 128 185 621.6 0 185 129 394 621.6 0 394 130 48 621.6 0 48 131 55 621.6 0 55 132 98 621.6 0 98 133 57 621.6 0 57 134 62 621.6 0 62 135 42 621.6 0 42 136 43 621.6 0 43 137 47 621.6 0 47 138 63 621.6 0 63 139 95 621.6 0 95 140 110 621.6 0 110 141 129 621.6 0 129 142 195 621.6 0 195 143 502 621.6 0 502 144 5077 621.6 0 5077 145 2482 621.6 0 2482 146 4403 621.6 0 4403 147 62 621.6 0 62 148 55 621.6 0 55 149 74 621.6 0 74 150 64 621.6 0 64 151 302 621.6 0 302 152 198 621.6 0 198 153 464 621.6 0 464 154 191 621.6 0 191 155 85 621.6 0 85 156 212 621.6 0 212 157 167 621.6 0 167 158 1552 621.6 0 1552 159 64 621.6 0 64 160 55 621.6 0 55 161 72 621.6 0 72 162 44 621.6 0 44 163 62 621.6 0 62 164 38 621.6 0 38 165 49 621.6 0 49 166 61 621.6 0 61 167 45 621.6 0 45 168 30 621.6 0 30 169 35 621.6 0 35 170 60 621.6 0 60 171 68 621.6 0 68 172 106 621.6 0 106 173 93 621.6 0 93 174 62 621.6 0 62 175 115 621.6 0 115 176 76 621.6 0 76 177 47 621.6 0 47 178 54 621.6 0 54 179 161 621.6 0 161 180 111 621.6 0 111 181 111 621.6 0 111 182 44 621.6 0 44 183 29 621.6 0 29 184 54 621.6 0 54 185 95 621.6 0 95 186 95 621.6 0 95 187 73 621.6 0 73 188 52 621.6 0 52 189 50 621.6 0 50 190 98 621.6 0 98 191 117 621.6 0 117 192 376 621.6 0 376 193 66 621.6 0 66 194 74 621.6 0 74 195 38 621.6 0 38 196 64 621.6 0 64 197 85 621.6 0 85 198 194 621.6 0 194 199 105 621.6 0 105 200 117 621.6 0 117 201 55 621.6 0 55 202 44 621.6 0 44 203 62 621.6 0 62 204 94 621.6 0 94 205 64 621.6 0 64 206 60 621.6 0 60 207 63 621.6 0 63 208 54 621.6 0 54 209 33 621.6 0 33 210 42 621.6 0 42 211 32 621.6 0 32 212 56 621.6 0 56 213 51 621.6 0 51 214 47 621.6 0 47 215 47 621.6 0 47 216 38 621.6 0 38 217 54 621.6 0 54 218 45 621.6 0 45 219 49 621.6 0 49 220 38 621.6 0 38 221 40 621.6 0 40 222 33 621.6 0 33 223 46 621.6 0 46 224 42 621.6 0 42 225 101 621.6 0 101 226 91 621.6 0 91 227 103 621.6 0 103 228 126 621.6 0 126 229 95 621.6 0 95 230 198 621.6 0 198 231 213 621.6 0 213 232 141 621.6 0 141 233 149 621.6 0 149 234 172 621.6 0 172 235 544 621.6 0 544 236 765 621.6 0 765 237 728 621.6 0 728 238 682 621.6 0 682 239 196 621.6 0 196 240 647 621.6 0 647 241 510 621.6 0 510 242 1437 621.6 0 1437 243 175 621.6 0 175 244 123 621.6 0 123 245 90 621.6 0 90 246 131 621.6 0 131 247 312 621.6 0 312 248 211 621.6 0 211 249 329 621.6 0 329 250 283 621.6 0 283 251 260 621.6 0 260 252 382 621.6 0 382 253 150 621.6 0 150 254 103 621.6 0 103 255 147 621.6 0 147 256 226 621.6 0 226 257 269 621.6 0 269 258 467 621.6 0 467 259 1106 621.6 0 1106 260 7690 621.6 0 7690 261 7689 621.6 0 7689 262 16578 621.6 0 16578 263 4145 621.6 0 4145 264 107 621.6 0 107 265 74 621.6 0 74 266 93 621.6 0 93 267 153 621.6 0 153 268 153 621.6 0 153 269 128 621.6 0 128 270 172 621.6 0 172 271 200 621.6 0 200 272 311 621.6 0 311 273 79 621.6 0 79 274 66 621.6 0 66 275 67 621.6 0 67 276 66 621.6 0 66 277 126 621.6 0 126 278 109 621.6 0 109 279 192 621.6 0 192 280 292 621.6 0 292 281 326 621.6 0 326 282 348 621.6 0 348 283 206 621.6 0 206 284 325 621.6 0 325 285 117 621.6 0 117 286 149 621.6 0 149 287 144 621.6 0 144 288 400 621.6 0 400 289 256 621.6 0 256 290 325 621.6 0 325 291 501 621.6 0 501 292 587 621.6 0 587 293 1025 621.6 0 1025 294 642 621.6 0 642 295 480 621.6 0 480 296 511 621.6 0 511 297 124 621.6 0 124 298 78 621.6 0 78 299 89 621.6 0 89 300 67 621.6 0 67 301 78 621.6 0 78 Finished in 3.53 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 77,705 Reads with adapters: 25,373 (32.7%) Reads that were too short: 65 (0.1%) Reads written (passing filters): 25,308 (32.6%) Total basepairs processed: 19,026,838 bp Total written (filtered): 6,978,848 bp (36.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 25373 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 10 0.0 2 0 0 0 10 15 16 0.0 3 0 0 11 5 16 220 0.0 3 0 181 22 17 17 24814 0.0 3 23279 1239 233 63 18 72 0.0 3 2 52 9 9 19 52 0.0 3 0 0 30 22 20 72 0.0 3 0 0 0 72 22 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 26 2 0.0 3 1 0 0 1 27 1 0.0 3 0 0 0 1 28 2 0.0 3 0 0 0 2 32 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 47 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 0 1 62 3 0.0 3 0 0 0 3 65 3 0.0 3 0 0 0 3 66 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 1 76 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 3 0.0 3 0 0 0 3 81 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 88 2 0.0 3 0 0 0 2 91 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 98 2 0.0 3 0 0 0 2 100 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 2 0.0 3 0 0 0 2 107 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 1 142 3 0.0 3 0 0 0 3 147 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 2 0.0 3 0 0 0 2 151 4 0.0 3 0 0 0 4 152 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 155 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 160 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 171 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 186 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 198 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 205 2 0.0 3 0 0 1 1 206 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 0 2 225 1 0.0 3 0 0 0 1 235 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 4 0.0 3 0 0 0 4 283 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.88 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 25,308 Reads with adapters: 148 (0.6%) Reads written (passing filters): 25,308 (100.0%) Total basepairs processed: 6,978,848 bp Total written (filtered): 6,978,339 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 148 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 29.7% C: 9.5% G: 52.7% T: 8.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 86 395.4 0 86 4 59 98.9 0 59 5 3 24.7 0 3