This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCGCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.30 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 97,550 (3.8%) Reads that were too short: 23,225 (0.9%) Reads written (passing filters): 74,325 (2.9%) Total basepairs processed: 766,381,518 bp Total written (filtered): 18,702,432 bp (2.4%) === Adapter 1 === Sequence: TCCGCT; Type: regular 5'; Length: 6; Trimmed: 97550 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 310 621.6 0 310 7 1902 621.6 0 1902 8 21442 621.6 0 21442 9 26104 621.6 0 26104 10 37 621.6 0 37 11 19 621.6 0 19 12 15 621.6 0 15 13 16 621.6 0 16 14 22 621.6 0 22 15 14 621.6 0 14 16 14 621.6 0 14 17 20 621.6 0 20 18 7 621.6 0 7 19 14 621.6 0 14 20 12 621.6 0 12 21 14 621.6 0 14 22 8 621.6 0 8 23 13 621.6 0 13 24 19 621.6 0 19 25 18 621.6 0 18 26 13 621.6 0 13 27 13 621.6 0 13 28 20 621.6 0 20 29 22 621.6 0 22 30 18 621.6 0 18 31 17 621.6 0 17 32 23 621.6 0 23 33 25 621.6 0 25 34 29 621.6 0 29 35 32 621.6 0 32 36 30 621.6 0 30 37 156 621.6 0 156 38 77 621.6 0 77 39 132 621.6 0 132 40 22 621.6 0 22 41 14 621.6 0 14 42 28 621.6 0 28 43 17 621.6 0 17 44 18 621.6 0 18 45 23 621.6 0 23 46 20 621.6 0 20 47 11 621.6 0 11 48 24 621.6 0 24 49 25 621.6 0 25 50 31 621.6 0 31 51 18 621.6 0 18 52 146 621.6 0 146 53 83 621.6 0 83 54 132 621.6 0 132 55 18 621.6 0 18 56 11 621.6 0 11 57 16 621.6 0 16 58 12 621.6 0 12 59 17 621.6 0 17 60 29 621.6 0 29 61 38 621.6 0 38 62 940 621.6 0 940 63 99 621.6 0 99 64 18 621.6 0 18 65 22 621.6 0 22 66 14 621.6 0 14 67 33 621.6 0 33 68 23 621.6 0 23 69 12 621.6 0 12 70 20 621.6 0 20 71 27 621.6 0 27 72 44 621.6 0 44 73 62 621.6 0 62 74 9 621.6 0 9 75 19 621.6 0 19 76 22 621.6 0 22 77 16 621.6 0 16 78 20 621.6 0 20 79 25 621.6 0 25 80 12 621.6 0 12 81 51 621.6 0 51 82 18 621.6 0 18 83 21 621.6 0 21 84 81 621.6 0 81 85 23 621.6 0 23 86 80 621.6 0 80 87 22 621.6 0 22 88 14 621.6 0 14 89 33 621.6 0 33 90 35 621.6 0 35 91 26 621.6 0 26 92 39 621.6 0 39 93 33 621.6 0 33 94 31 621.6 0 31 95 51 621.6 0 51 96 35 621.6 0 35 97 56 621.6 0 56 98 16 621.6 0 16 99 17 621.6 0 17 100 19 621.6 0 19 101 20 621.6 0 20 102 14 621.6 0 14 103 29 621.6 0 29 104 81 621.6 0 81 105 67 621.6 0 67 106 99 621.6 0 99 107 46 621.6 0 46 108 103 621.6 0 103 109 66 621.6 0 66 110 125 621.6 0 125 111 60 621.6 0 60 112 80 621.6 0 80 113 72 621.6 0 72 114 64 621.6 0 64 115 43 621.6 0 43 116 30 621.6 0 30 117 58 621.6 0 58 118 50 621.6 0 50 119 40 621.6 0 40 120 32 621.6 0 32 121 14 621.6 0 14 122 22 621.6 0 22 123 22 621.6 0 22 124 27 621.6 0 27 125 47 621.6 0 47 126 88 621.6 0 88 127 283 621.6 0 283 128 88 621.6 0 88 129 191 621.6 0 191 130 35 621.6 0 35 131 50 621.6 0 50 132 88 621.6 0 88 133 38 621.6 0 38 134 34 621.6 0 34 135 24 621.6 0 24 136 37 621.6 0 37 137 39 621.6 0 39 138 49 621.6 0 49 139 79 621.6 0 79 140 112 621.6 0 112 141 102 621.6 0 102 142 222 621.6 0 222 143 527 621.6 0 527 144 6256 621.6 0 6256 145 3043 621.6 0 3043 146 5259 621.6 0 5259 147 39 621.6 0 39 148 26 621.6 0 26 149 48 621.6 0 48 150 33 621.6 0 33 151 91 621.6 0 91 152 58 621.6 0 58 153 115 621.6 0 115 154 53 621.6 0 53 155 26 621.6 0 26 156 78 621.6 0 78 157 61 621.6 0 61 158 628 621.6 0 628 159 32 621.6 0 32 160 23 621.6 0 23 161 26 621.6 0 26 162 26 621.6 0 26 163 29 621.6 0 29 164 29 621.6 0 29 165 21 621.6 0 21 166 21 621.6 0 21 167 23 621.6 0 23 168 19 621.6 0 19 169 14 621.6 0 14 170 21 621.6 0 21 171 30 621.6 0 30 172 41 621.6 0 41 173 28 621.6 0 28 174 27 621.6 0 27 175 17 621.6 0 17 176 21 621.6 0 21 177 25 621.6 0 25 178 26 621.6 0 26 179 91 621.6 0 91 180 58 621.6 0 58 181 41 621.6 0 41 182 31 621.6 0 31 183 24 621.6 0 24 184 22 621.6 0 22 185 32 621.6 0 32 186 48 621.6 0 48 187 37 621.6 0 37 188 23 621.6 0 23 189 24 621.6 0 24 190 57 621.6 0 57 191 113 621.6 0 113 192 212 621.6 0 212 193 27 621.6 0 27 194 24 621.6 0 24 195 18 621.6 0 18 196 33 621.6 0 33 197 33 621.6 0 33 198 73 621.6 0 73 199 22 621.6 0 22 200 39 621.6 0 39 201 15 621.6 0 15 202 13 621.6 0 13 203 23 621.6 0 23 204 42 621.6 0 42 205 23 621.6 0 23 206 28 621.6 0 28 207 25 621.6 0 25 208 11 621.6 0 11 209 24 621.6 0 24 210 14 621.6 0 14 211 5 621.6 0 5 212 14 621.6 0 14 213 23 621.6 0 23 214 27 621.6 0 27 215 27 621.6 0 27 216 14 621.6 0 14 217 27 621.6 0 27 218 14 621.6 0 14 219 36 621.6 0 36 220 23 621.6 0 23 221 19 621.6 0 19 222 21 621.6 0 21 223 18 621.6 0 18 224 36 621.6 0 36 225 36 621.6 0 36 226 30 621.6 0 30 227 38 621.6 0 38 228 34 621.6 0 34 229 51 621.6 0 51 230 101 621.6 0 101 231 147 621.6 0 147 232 84 621.6 0 84 233 53 621.6 0 53 234 70 621.6 0 70 235 182 621.6 0 182 236 247 621.6 0 247 237 240 621.6 0 240 238 189 621.6 0 189 239 65 621.6 0 65 240 243 621.6 0 243 241 172 621.6 0 172 242 373 621.6 0 373 243 64 621.6 0 64 244 47 621.6 0 47 245 36 621.6 0 36 246 54 621.6 0 54 247 107 621.6 0 107 248 65 621.6 0 65 249 129 621.6 0 129 250 110 621.6 0 110 251 129 621.6 0 129 252 170 621.6 0 170 253 61 621.6 0 61 254 33 621.6 0 33 255 53 621.6 0 53 256 75 621.6 0 75 257 79 621.6 0 79 258 165 621.6 0 165 259 428 621.6 0 428 260 3607 621.6 0 3607 261 3238 621.6 0 3238 262 6473 621.6 0 6473 263 1871 621.6 0 1871 264 67 621.6 0 67 265 28 621.6 0 28 266 37 621.6 0 37 267 54 621.6 0 54 268 59 621.6 0 59 269 44 621.6 0 44 270 89 621.6 0 89 271 52 621.6 0 52 272 180 621.6 0 180 273 40 621.6 0 40 274 31 621.6 0 31 275 27 621.6 0 27 276 37 621.6 0 37 277 64 621.6 0 64 278 54 621.6 0 54 279 78 621.6 0 78 280 87 621.6 0 87 281 93 621.6 0 93 282 86 621.6 0 86 283 184 621.6 0 184 284 379 621.6 0 379 285 97 621.6 0 97 286 49 621.6 0 49 287 46 621.6 0 46 288 177 621.6 0 177 289 112 621.6 0 112 290 91 621.6 0 91 291 145 621.6 0 145 292 131 621.6 0 131 293 224 621.6 0 224 294 122 621.6 0 122 295 94 621.6 0 94 296 108 621.6 0 108 297 50 621.6 0 50 298 36 621.6 0 36 299 40 621.6 0 40 300 27 621.6 0 27 301 50 621.6 0 50 Finished in 3.65 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 74,325 Reads with adapters: 27,414 (36.9%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 27,406 (36.9%) Total basepairs processed: 18,702,432 bp Total written (filtered): 7,442,348 bp (39.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 27414 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 11 0.0 3 0 0 11 19 347 0.0 3 0 341 6 20 26813 0.0 4 26393 409 11 21 46 0.0 4 9 35 2 22 6 0.0 4 2 1 3 23 54 0.0 4 32 0 1 19 2 47 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 126 2 0.0 4 0 0 0 0 2 127 123 0.0 4 0 0 0 0 123 128 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 152 1 0.0 4 1 153 2 0.0 4 0 0 0 0 2 202 1 0.0 4 0 0 1 216 1 0.0 4 0 0 0 0 1 230 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.97 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 27,406 Reads with adapters: 3,662 (13.4%) Reads written (passing filters): 27,406 (100.0%) Total basepairs processed: 7,442,348 bp Total written (filtered): 7,406,211 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3662 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 2.8% G: 94.6% T: 1.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 7 428.2 0 7 4 2 107.1 0 2 5 1 26.8 0 1 6 1 6.7 0 1 7 1 1.7 0 1 9 1695 0.1 0 64 1631 10 1585 0.0 1 54 1531 11 356 0.0 1 4 352 12 3 0.0 1 0 3 13 4 0.0 1 0 4 18 1 0.0 1 1 23 1 0.0 1 1 107 1 0.0 1 0 1 208 3 0.0 1 0 3 209 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.