This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.73 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 135,981 (5.3%) Reads that were too short: 17,442 (0.7%) Reads written (passing filters): 118,539 (4.7%) Total basepairs processed: 766,381,518 bp Total written (filtered): 31,702,202 bp (4.1%) === Adapter 1 === Sequence: ACAGGT; Type: regular 5'; Length: 6; Trimmed: 135981 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 970 621.6 0 970 7 48300 621.6 0 48300 8 40702 621.6 0 40702 9 103 621.6 0 103 10 55 621.6 0 55 11 73 621.6 0 73 12 224 621.6 0 224 13 50 621.6 0 50 14 43 621.6 0 43 15 42 621.6 0 42 16 47 621.6 0 47 17 52 621.6 0 52 18 81 621.6 0 81 19 75 621.6 0 75 20 154 621.6 0 154 21 71 621.6 0 71 22 72 621.6 0 72 23 56 621.6 0 56 24 66 621.6 0 66 25 61 621.6 0 61 26 87 621.6 0 87 27 67 621.6 0 67 28 66 621.6 0 66 29 62 621.6 0 62 30 58 621.6 0 58 31 55 621.6 0 55 32 69 621.6 0 69 33 49 621.6 0 49 34 93 621.6 0 93 35 68 621.6 0 68 36 52 621.6 0 52 37 62 621.6 0 62 38 60 621.6 0 60 39 81 621.6 0 81 40 66 621.6 0 66 41 56 621.6 0 56 42 48 621.6 0 48 43 65 621.6 0 65 44 73 621.6 0 73 45 68 621.6 0 68 46 71 621.6 0 71 47 95 621.6 0 95 48 68 621.6 0 68 49 52 621.6 0 52 50 102 621.6 0 102 51 47 621.6 0 47 52 43 621.6 0 43 53 72 621.6 0 72 54 99 621.6 0 99 55 82 621.6 0 82 56 56 621.6 0 56 57 56 621.6 0 56 58 73 621.6 0 73 59 89 621.6 0 89 60 61 621.6 0 61 61 64 621.6 0 64 62 63 621.6 0 63 63 65 621.6 0 65 64 59 621.6 0 59 65 69 621.6 0 69 66 98 621.6 0 98 67 113 621.6 0 113 68 113 621.6 0 113 69 154 621.6 0 154 70 230 621.6 0 230 71 1943 621.6 0 1943 72 1821 621.6 0 1821 73 1434 621.6 0 1434 74 72 621.6 0 72 75 43 621.6 0 43 76 74 621.6 0 74 77 127 621.6 0 127 78 78 621.6 0 78 79 168 621.6 0 168 80 59 621.6 0 59 81 49 621.6 0 49 82 77 621.6 0 77 83 86 621.6 0 86 84 84 621.6 0 84 85 79 621.6 0 79 86 101 621.6 0 101 87 124 621.6 0 124 88 69 621.6 0 69 89 59 621.6 0 59 90 117 621.6 0 117 91 77 621.6 0 77 92 65 621.6 0 65 93 114 621.6 0 114 94 122 621.6 0 122 95 173 621.6 0 173 96 881 621.6 0 881 97 371 621.6 0 371 98 748 621.6 0 748 99 161 621.6 0 161 100 69 621.6 0 69 101 65 621.6 0 65 102 49 621.6 0 49 103 72 621.6 0 72 104 51 621.6 0 51 105 58 621.6 0 58 106 66 621.6 0 66 107 60 621.6 0 60 108 77 621.6 0 77 109 80 621.6 0 80 110 52 621.6 0 52 111 66 621.6 0 66 112 80 621.6 0 80 113 81 621.6 0 81 114 84 621.6 0 84 115 78 621.6 0 78 116 85 621.6 0 85 117 88 621.6 0 88 118 92 621.6 0 92 119 96 621.6 0 96 120 73 621.6 0 73 121 75 621.6 0 75 122 70 621.6 0 70 123 73 621.6 0 73 124 127 621.6 0 127 125 191 621.6 0 191 126 218 621.6 0 218 127 135 621.6 0 135 128 158 621.6 0 158 129 174 621.6 0 174 130 171 621.6 0 171 131 107 621.6 0 107 132 106 621.6 0 106 133 155 621.6 0 155 134 100 621.6 0 100 135 177 621.6 0 177 136 112 621.6 0 112 137 106 621.6 0 106 138 105 621.6 0 105 139 337 621.6 0 337 140 96 621.6 0 96 141 108 621.6 0 108 142 100 621.6 0 100 143 117 621.6 0 117 144 76 621.6 0 76 145 92 621.6 0 92 146 93 621.6 0 93 147 70 621.6 0 70 148 81 621.6 0 81 149 72 621.6 0 72 150 72 621.6 0 72 151 107 621.6 0 107 152 78 621.6 0 78 153 114 621.6 0 114 154 117 621.6 0 117 155 119 621.6 0 119 156 104 621.6 0 104 157 74 621.6 0 74 158 73 621.6 0 73 159 88 621.6 0 88 160 90 621.6 0 90 161 79 621.6 0 79 162 77 621.6 0 77 163 65 621.6 0 65 164 95 621.6 0 95 165 107 621.6 0 107 166 97 621.6 0 97 167 92 621.6 0 92 168 80 621.6 0 80 169 80 621.6 0 80 170 101 621.6 0 101 171 93 621.6 0 93 172 140 621.6 0 140 173 365 621.6 0 365 174 189 621.6 0 189 175 330 621.6 0 330 176 122 621.6 0 122 177 77 621.6 0 77 178 75 621.6 0 75 179 105 621.6 0 105 180 120 621.6 0 120 181 91 621.6 0 91 182 78 621.6 0 78 183 82 621.6 0 82 184 104 621.6 0 104 185 115 621.6 0 115 186 146 621.6 0 146 187 133 621.6 0 133 188 83 621.6 0 83 189 95 621.6 0 95 190 142 621.6 0 142 191 233 621.6 0 233 192 656 621.6 0 656 193 883 621.6 0 883 194 1737 621.6 0 1737 195 710 621.6 0 710 196 77 621.6 0 77 197 61 621.6 0 61 198 103 621.6 0 103 199 98 621.6 0 98 200 97 621.6 0 97 201 94 621.6 0 94 202 71 621.6 0 71 203 112 621.6 0 112 204 96 621.6 0 96 205 85 621.6 0 85 206 118 621.6 0 118 207 194 621.6 0 194 208 110 621.6 0 110 209 152 621.6 0 152 210 191 621.6 0 191 211 181 621.6 0 181 212 156 621.6 0 156 213 94 621.6 0 94 214 97 621.6 0 97 215 193 621.6 0 193 216 313 621.6 0 313 217 289 621.6 0 289 218 88 621.6 0 88 219 130 621.6 0 130 220 145 621.6 0 145 221 136 621.6 0 136 222 91 621.6 0 91 223 78 621.6 0 78 224 78 621.6 0 78 225 108 621.6 0 108 226 133 621.6 0 133 227 124 621.6 0 124 228 84 621.6 0 84 229 90 621.6 0 90 230 101 621.6 0 101 231 96 621.6 0 96 232 97 621.6 0 97 233 113 621.6 0 113 234 88 621.6 0 88 235 107 621.6 0 107 236 134 621.6 0 134 237 144 621.6 0 144 238 124 621.6 0 124 239 82 621.6 0 82 240 100 621.6 0 100 241 114 621.6 0 114 242 115 621.6 0 115 243 91 621.6 0 91 244 84 621.6 0 84 245 107 621.6 0 107 246 115 621.6 0 115 247 127 621.6 0 127 248 113 621.6 0 113 249 154 621.6 0 154 250 101 621.6 0 101 251 86 621.6 0 86 252 83 621.6 0 83 253 142 621.6 0 142 254 181 621.6 0 181 255 199 621.6 0 199 256 137 621.6 0 137 257 116 621.6 0 116 258 155 621.6 0 155 259 177 621.6 0 177 260 148 621.6 0 148 261 114 621.6 0 114 262 211 621.6 0 211 263 114 621.6 0 114 264 108 621.6 0 108 265 95 621.6 0 95 266 136 621.6 0 136 267 199 621.6 0 199 268 154 621.6 0 154 269 138 621.6 0 138 270 194 621.6 0 194 271 227 621.6 0 227 272 2153 621.6 0 2153 273 953 621.6 0 953 274 1284 621.6 0 1284 275 171 621.6 0 171 276 103 621.6 0 103 277 81 621.6 0 81 278 151 621.6 0 151 279 223 621.6 0 223 280 204 621.6 0 204 281 151 621.6 0 151 282 137 621.6 0 137 283 87 621.6 0 87 284 121 621.6 0 121 285 119 621.6 0 119 286 144 621.6 0 144 287 114 621.6 0 114 288 132 621.6 0 132 289 139 621.6 0 139 290 163 621.6 0 163 291 146 621.6 0 146 292 140 621.6 0 140 293 137 621.6 0 137 294 226 621.6 0 226 295 195 621.6 0 195 296 227 621.6 0 227 297 129 621.6 0 129 298 90 621.6 0 90 299 123 621.6 0 123 300 138 621.6 0 138 301 103 621.6 0 103 Finished in 5.51 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 118,539 Reads with adapters: 47,722 (40.3%) Reads that were too short: 100 (0.1%) Reads written (passing filters): 47,622 (40.2%) Total basepairs processed: 31,702,202 bp Total written (filtered): 13,178,732 bp (41.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 47722 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 6 0.0 2 0 0 0 6 15 36 0.0 3 0 0 13 23 16 373 0.0 3 0 307 41 25 17 46761 0.0 3 43485 2667 498 111 18 110 0.0 3 1 79 9 21 19 133 0.0 3 0 0 71 62 20 124 0.0 3 0 0 0 124 21 1 0.0 3 1 22 1 0.0 3 0 0 0 1 24 1 0.0 3 1 25 1 0.0 3 0 0 0 1 29 1 0.0 3 0 0 0 1 32 2 0.0 3 0 0 1 1 33 1 0.0 3 0 0 0 1 34 2 0.0 3 1 0 0 1 35 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 41 1 0.0 3 0 0 1 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 2 0.0 3 0 0 0 2 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 1 50 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 9 0.0 3 0 0 1 8 54 1 0.0 3 0 0 1 55 1 0.0 3 0 0 0 1 56 2 0.0 3 0 0 0 2 59 1 0.0 3 0 0 0 1 61 3 0.0 3 0 0 0 3 64 3 0.0 3 0 0 0 3 65 1 0.0 3 0 0 1 66 1 0.0 3 0 0 0 1 67 8 0.0 3 0 0 0 8 68 3 0.0 3 0 0 0 3 71 2 0.0 3 0 0 0 2 79 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 89 2 0.0 3 0 0 1 1 94 1 0.0 3 0 0 0 1 95 2 0.0 3 0 0 0 2 96 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 99 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 105 3 0.0 3 0 0 0 3 106 2 0.0 3 0 0 0 2 115 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 132 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 152 2 0.0 3 0 0 0 2 155 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 5 0.0 3 0 0 1 4 165 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 1 167 1 0.0 3 0 0 0 1 169 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 190 2 0.0 3 0 0 0 2 191 5 0.0 3 0 0 0 5 192 1 0.0 3 0 0 0 1 194 4 0.0 3 0 0 0 4 195 1 0.0 3 0 0 0 1 205 6 0.0 3 0 0 0 6 207 5 0.0 3 0 0 0 5 210 1 0.0 3 0 0 0 1 214 3 0.0 3 0 0 0 3 217 3 0.0 3 0 0 0 3 218 2 0.0 3 0 0 0 2 221 2 0.0 3 0 0 0 2 225 2 0.0 3 0 0 0 2 231 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 267 1 0.0 3 0 0 1 278 1 0.0 3 0 0 0 1 279 7 0.0 3 0 0 0 7 280 4 0.0 3 0 0 1 3 281 11 0.0 3 0 0 0 11 282 7 0.0 3 0 0 0 7 287 1 0.0 3 0 0 0 1 288 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.68 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 47,622 Reads with adapters: 148 (0.3%) Reads written (passing filters): 47,622 (100.0%) Total basepairs processed: 13,178,732 bp Total written (filtered): 13,178,258 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 148 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 19.6% C: 28.4% G: 41.9% T: 10.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 120 744.1 0 120 4 26 186.0 0 26 5 2 46.5 0 2