This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.70 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 125,972 (4.9%) Reads that were too short: 8,875 (0.3%) Reads written (passing filters): 117,097 (4.6%) Total basepairs processed: 766,381,518 bp Total written (filtered): 32,127,666 bp (4.2%) === Adapter 1 === Sequence: ACAGGT; Type: regular 5'; Length: 6; Trimmed: 125972 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 799 621.6 0 799 7 44912 621.6 0 44912 8 48406 621.6 0 48406 9 75 621.6 0 75 10 58 621.6 0 58 11 47 621.6 0 47 12 124 621.6 0 124 13 62 621.6 0 62 14 43 621.6 0 43 15 49 621.6 0 49 16 39 621.6 0 39 17 47 621.6 0 47 18 87 621.6 0 87 19 59 621.6 0 59 20 48 621.6 0 48 21 52 621.6 0 52 22 67 621.6 0 67 23 54 621.6 0 54 24 62 621.6 0 62 25 48 621.6 0 48 26 73 621.6 0 73 27 45 621.6 0 45 28 47 621.6 0 47 29 38 621.6 0 38 30 64 621.6 0 64 31 64 621.6 0 64 32 55 621.6 0 55 33 40 621.6 0 40 34 62 621.6 0 62 35 56 621.6 0 56 36 85 621.6 0 85 37 73 621.6 0 73 38 60 621.6 0 60 39 71 621.6 0 71 40 71 621.6 0 71 41 70 621.6 0 70 42 58 621.6 0 58 43 62 621.6 0 62 44 69 621.6 0 69 45 47 621.6 0 47 46 61 621.6 0 61 47 69 621.6 0 69 48 52 621.6 0 52 49 47 621.6 0 47 50 130 621.6 0 130 51 69 621.6 0 69 52 65 621.6 0 65 53 67 621.6 0 67 54 89 621.6 0 89 55 67 621.6 0 67 56 64 621.6 0 64 57 65 621.6 0 65 58 63 621.6 0 63 59 57 621.6 0 57 60 55 621.6 0 55 61 50 621.6 0 50 62 47 621.6 0 47 63 66 621.6 0 66 64 63 621.6 0 63 65 65 621.6 0 65 66 79 621.6 0 79 67 108 621.6 0 108 68 105 621.6 0 105 69 147 621.6 0 147 70 200 621.6 0 200 71 2245 621.6 0 2245 72 2205 621.6 0 2205 73 1662 621.6 0 1662 74 68 621.6 0 68 75 47 621.6 0 47 76 45 621.6 0 45 77 61 621.6 0 61 78 56 621.6 0 56 79 113 621.6 0 113 80 53 621.6 0 53 81 58 621.6 0 58 82 51 621.6 0 51 83 57 621.6 0 57 84 53 621.6 0 53 85 54 621.6 0 54 86 58 621.6 0 58 87 57 621.6 0 57 88 52 621.6 0 52 89 52 621.6 0 52 90 107 621.6 0 107 91 71 621.6 0 71 92 63 621.6 0 63 93 114 621.6 0 114 94 81 621.6 0 81 95 159 621.6 0 159 96 499 621.6 0 499 97 438 621.6 0 438 98 794 621.6 0 794 99 171 621.6 0 171 100 63 621.6 0 63 101 50 621.6 0 50 102 44 621.6 0 44 103 56 621.6 0 56 104 54 621.6 0 54 105 49 621.6 0 49 106 62 621.6 0 62 107 51 621.6 0 51 108 55 621.6 0 55 109 74 621.6 0 74 110 55 621.6 0 55 111 40 621.6 0 40 112 83 621.6 0 83 113 75 621.6 0 75 114 69 621.6 0 69 115 77 621.6 0 77 116 60 621.6 0 60 117 75 621.6 0 75 118 86 621.6 0 86 119 71 621.6 0 71 120 53 621.6 0 53 121 59 621.6 0 59 122 50 621.6 0 50 123 57 621.6 0 57 124 94 621.6 0 94 125 134 621.6 0 134 126 118 621.6 0 118 127 84 621.6 0 84 128 96 621.6 0 96 129 92 621.6 0 92 130 106 621.6 0 106 131 75 621.6 0 75 132 57 621.6 0 57 133 67 621.6 0 67 134 69 621.6 0 69 135 97 621.6 0 97 136 80 621.6 0 80 137 95 621.6 0 95 138 72 621.6 0 72 139 135 621.6 0 135 140 62 621.6 0 62 141 63 621.6 0 63 142 71 621.6 0 71 143 74 621.6 0 74 144 75 621.6 0 75 145 70 621.6 0 70 146 57 621.6 0 57 147 58 621.6 0 58 148 63 621.6 0 63 149 58 621.6 0 58 150 62 621.6 0 62 151 83 621.6 0 83 152 66 621.6 0 66 153 50 621.6 0 50 154 87 621.6 0 87 155 63 621.6 0 63 156 67 621.6 0 67 157 73 621.6 0 73 158 65 621.6 0 65 159 57 621.6 0 57 160 85 621.6 0 85 161 71 621.6 0 71 162 59 621.6 0 59 163 68 621.6 0 68 164 57 621.6 0 57 165 69 621.6 0 69 166 66 621.6 0 66 167 69 621.6 0 69 168 52 621.6 0 52 169 51 621.6 0 51 170 72 621.6 0 72 171 76 621.6 0 76 172 89 621.6 0 89 173 146 621.6 0 146 174 110 621.6 0 110 175 152 621.6 0 152 176 72 621.6 0 72 177 54 621.6 0 54 178 58 621.6 0 58 179 68 621.6 0 68 180 79 621.6 0 79 181 67 621.6 0 67 182 64 621.6 0 64 183 58 621.6 0 58 184 72 621.6 0 72 185 71 621.6 0 71 186 74 621.6 0 74 187 80 621.6 0 80 188 60 621.6 0 60 189 78 621.6 0 78 190 102 621.6 0 102 191 135 621.6 0 135 192 394 621.6 0 394 193 523 621.6 0 523 194 868 621.6 0 868 195 363 621.6 0 363 196 68 621.6 0 68 197 49 621.6 0 49 198 48 621.6 0 48 199 82 621.6 0 82 200 67 621.6 0 67 201 46 621.6 0 46 202 61 621.6 0 61 203 56 621.6 0 56 204 50 621.6 0 50 205 52 621.6 0 52 206 76 621.6 0 76 207 96 621.6 0 96 208 68 621.6 0 68 209 57 621.6 0 57 210 75 621.6 0 75 211 85 621.6 0 85 212 57 621.6 0 57 213 62 621.6 0 62 214 64 621.6 0 64 215 72 621.6 0 72 216 82 621.6 0 82 217 121 621.6 0 121 218 53 621.6 0 53 219 65 621.6 0 65 220 41 621.6 0 41 221 48 621.6 0 48 222 48 621.6 0 48 223 56 621.6 0 56 224 56 621.6 0 56 225 104 621.6 0 104 226 78 621.6 0 78 227 98 621.6 0 98 228 52 621.6 0 52 229 87 621.6 0 87 230 64 621.6 0 64 231 43 621.6 0 43 232 54 621.6 0 54 233 83 621.6 0 83 234 51 621.6 0 51 235 75 621.6 0 75 236 82 621.6 0 82 237 61 621.6 0 61 238 74 621.6 0 74 239 66 621.6 0 66 240 49 621.6 0 49 241 54 621.6 0 54 242 54 621.6 0 54 243 63 621.6 0 63 244 67 621.6 0 67 245 43 621.6 0 43 246 62 621.6 0 62 247 65 621.6 0 65 248 73 621.6 0 73 249 91 621.6 0 91 250 75 621.6 0 75 251 70 621.6 0 70 252 57 621.6 0 57 253 83 621.6 0 83 254 107 621.6 0 107 255 84 621.6 0 84 256 64 621.6 0 64 257 94 621.6 0 94 258 82 621.6 0 82 259 74 621.6 0 74 260 80 621.6 0 80 261 65 621.6 0 65 262 96 621.6 0 96 263 79 621.6 0 79 264 56 621.6 0 56 265 64 621.6 0 64 266 85 621.6 0 85 267 82 621.6 0 82 268 80 621.6 0 80 269 86 621.6 0 86 270 116 621.6 0 116 271 100 621.6 0 100 272 812 621.6 0 812 273 388 621.6 0 388 274 451 621.6 0 451 275 82 621.6 0 82 276 64 621.6 0 64 277 59 621.6 0 59 278 136 621.6 0 136 279 105 621.6 0 105 280 108 621.6 0 108 281 84 621.6 0 84 282 66 621.6 0 66 283 64 621.6 0 64 284 79 621.6 0 79 285 70 621.6 0 70 286 72 621.6 0 72 287 69 621.6 0 69 288 72 621.6 0 72 289 91 621.6 0 91 290 113 621.6 0 113 291 89 621.6 0 89 292 81 621.6 0 81 293 66 621.6 0 66 294 118 621.6 0 118 295 81 621.6 0 81 296 94 621.6 0 94 297 71 621.6 0 71 298 71 621.6 0 71 299 64 621.6 0 64 300 103 621.6 0 103 301 79 621.6 0 79 Finished in 6.00 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 117,097 Reads with adapters: 49,888 (42.6%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 49,877 (42.6%) Total basepairs processed: 32,127,666 bp Total written (filtered): 13,605,635 bp (42.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 49888 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 16 0.0 3 0 0 16 19 775 0.0 3 0 765 9 1 20 48820 0.0 4 48091 700 27 2 21 108 0.0 4 13 92 3 22 3 0.0 4 0 0 3 23 30 0.0 4 2 0 0 25 3 24 2 0.0 4 0 0 0 0 2 45 1 0.0 4 1 53 1 0.0 4 0 0 0 0 1 67 1 0.0 4 0 0 0 0 1 113 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 127 115 0.0 4 0 0 0 0 115 128 1 0.0 4 0 0 0 0 1 153 3 0.0 4 0 0 0 0 3 214 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 0 2 231 1 0.0 4 0 0 0 0 1 251 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.77 s (35 us/read; 1.69 M reads/minute). === Summary === Total reads processed: 49,877 Reads with adapters: 4,941 (9.9%) Reads written (passing filters): 49,877 (100.0%) Total basepairs processed: 13,605,635 bp Total written (filtered): 13,550,624 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 4941 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.1% C: 3.2% G: 94.0% T: 1.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 21 779.3 0 21 4 6 194.8 0 6 5 2 48.7 0 2 9 122 0.2 0 3 119 10 319 0.0 1 14 305 11 4452 0.0 1 15 4437 12 8 0.0 1 0 8 14 1 0.0 1 0 1 15 1 0.0 1 0 1 151 6 0.0 1 0 6 190 1 0.0 1 0 1 209 1 0.0 1 1 224 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.