This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCTGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.89 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 211,480 (8.3%) Reads that were too short: 31,876 (1.3%) Reads written (passing filters): 179,604 (7.1%) Total basepairs processed: 766,381,518 bp Total written (filtered): 32,428,283 bp (4.2%) === Adapter 1 === Sequence: CTCTGC; Type: regular 5'; Length: 6; Trimmed: 211480 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 345 621.6 0 345 7 8036 621.6 0 8036 8 355 621.6 0 355 9 6794 621.6 0 6794 10 57 621.6 0 57 11 54 621.6 0 54 12 32 621.6 0 32 13 47 621.6 0 47 14 131 621.6 0 131 15 53 621.6 0 53 16 94 621.6 0 94 17 39 621.6 0 39 18 40 621.6 0 40 19 37 621.6 0 37 20 30 621.6 0 30 21 74 621.6 0 74 22 56 621.6 0 56 23 64 621.6 0 64 24 82 621.6 0 82 25 77 621.6 0 77 26 40 621.6 0 40 27 57 621.6 0 57 28 51 621.6 0 51 29 42 621.6 0 42 30 47 621.6 0 47 31 50 621.6 0 50 32 51 621.6 0 51 33 51 621.6 0 51 34 64 621.6 0 64 35 45 621.6 0 45 36 65 621.6 0 65 37 55 621.6 0 55 38 65 621.6 0 65 39 68 621.6 0 68 40 61 621.6 0 61 41 69 621.6 0 69 42 74 621.6 0 74 43 57 621.6 0 57 44 51 621.6 0 51 45 61 621.6 0 61 46 87 621.6 0 87 47 54 621.6 0 54 48 70 621.6 0 70 49 52 621.6 0 52 50 55 621.6 0 55 51 75 621.6 0 75 52 68 621.6 0 68 53 68 621.6 0 68 54 62 621.6 0 62 55 66 621.6 0 66 56 158 621.6 0 158 57 171 621.6 0 171 58 319 621.6 0 319 59 543 621.6 0 543 60 174 621.6 0 174 61 330 621.6 0 330 62 56 621.6 0 56 63 60 621.6 0 60 64 74 621.6 0 74 65 77 621.6 0 77 66 56 621.6 0 56 67 101 621.6 0 101 68 51 621.6 0 51 69 83 621.6 0 83 70 71 621.6 0 71 71 56 621.6 0 56 72 87 621.6 0 87 73 118 621.6 0 118 74 84 621.6 0 84 75 66 621.6 0 66 76 70 621.6 0 70 77 192 621.6 0 192 78 47 621.6 0 47 79 63 621.6 0 63 80 57 621.6 0 57 81 55 621.6 0 55 82 67 621.6 0 67 83 105 621.6 0 105 84 116 621.6 0 116 85 161 621.6 0 161 86 109 621.6 0 109 87 169 621.6 0 169 88 86 621.6 0 86 89 119 621.6 0 119 90 124 621.6 0 124 91 147 621.6 0 147 92 91 621.6 0 91 93 69 621.6 0 69 94 102 621.6 0 102 95 89 621.6 0 89 96 107 621.6 0 107 97 279 621.6 0 279 98 89 621.6 0 89 99 128 621.6 0 128 100 127 621.6 0 127 101 120 621.6 0 120 102 156 621.6 0 156 103 121 621.6 0 121 104 102 621.6 0 102 105 114 621.6 0 114 106 486 621.6 0 486 107 351 621.6 0 351 108 673 621.6 0 673 109 321 621.6 0 321 110 518 621.6 0 518 111 543 621.6 0 543 112 1220 621.6 0 1220 113 2158 621.6 0 2158 114 27461 621.6 0 27461 115 15206 621.6 0 15206 116 25459 621.6 0 25459 117 211 621.6 0 211 118 139 621.6 0 139 119 65 621.6 0 65 120 83 621.6 0 83 121 54 621.6 0 54 122 37 621.6 0 37 123 80 621.6 0 80 124 63 621.6 0 63 125 80 621.6 0 80 126 195 621.6 0 195 127 273 621.6 0 273 128 160 621.6 0 160 129 201 621.6 0 201 130 159 621.6 0 159 131 141 621.6 0 141 132 85 621.6 0 85 133 76 621.6 0 76 134 79 621.6 0 79 135 147 621.6 0 147 136 290 621.6 0 290 137 144 621.6 0 144 138 131 621.6 0 131 139 105 621.6 0 105 140 423 621.6 0 423 141 899 621.6 0 899 142 634 621.6 0 634 143 686 621.6 0 686 144 307 621.6 0 307 145 430 621.6 0 430 146 849 621.6 0 849 147 6670 621.6 0 6670 148 3762 621.6 0 3762 149 10180 621.6 0 10180 150 427 621.6 0 427 151 721 621.6 0 721 152 1634 621.6 0 1634 153 10945 621.6 0 10945 154 10041 621.6 0 10041 155 20026 621.6 0 20026 156 401 621.6 0 401 157 244 621.6 0 244 158 235 621.6 0 235 159 127 621.6 0 127 160 134 621.6 0 134 161 150 621.6 0 150 162 193 621.6 0 193 163 166 621.6 0 166 164 140 621.6 0 140 165 97 621.6 0 97 166 82 621.6 0 82 167 197 621.6 0 197 168 548 621.6 0 548 169 394 621.6 0 394 170 641 621.6 0 641 171 166 621.6 0 166 172 75 621.6 0 75 173 48 621.6 0 48 174 65 621.6 0 65 175 74 621.6 0 74 176 48 621.6 0 48 177 60 621.6 0 60 178 87 621.6 0 87 179 65 621.6 0 65 180 72 621.6 0 72 181 117 621.6 0 117 182 121 621.6 0 121 183 845 621.6 0 845 184 619 621.6 0 619 185 875 621.6 0 875 186 680 621.6 0 680 187 292 621.6 0 292 188 126 621.6 0 126 189 66 621.6 0 66 190 111 621.6 0 111 191 143 621.6 0 143 192 82 621.6 0 82 193 74 621.6 0 74 194 57 621.6 0 57 195 41 621.6 0 41 196 50 621.6 0 50 197 72 621.6 0 72 198 77 621.6 0 77 199 83 621.6 0 83 200 69 621.6 0 69 201 73 621.6 0 73 202 93 621.6 0 93 203 117 621.6 0 117 204 97 621.6 0 97 205 58 621.6 0 58 206 54 621.6 0 54 207 59 621.6 0 59 208 45 621.6 0 45 209 54 621.6 0 54 210 34 621.6 0 34 211 64 621.6 0 64 212 62 621.6 0 62 213 84 621.6 0 84 214 70 621.6 0 70 215 186 621.6 0 186 216 436 621.6 0 436 217 331 621.6 0 331 218 558 621.6 0 558 219 52 621.6 0 52 220 44 621.6 0 44 221 42 621.6 0 42 222 54 621.6 0 54 223 49 621.6 0 49 224 62 621.6 0 62 225 66 621.6 0 66 226 120 621.6 0 120 227 109 621.6 0 109 228 105 621.6 0 105 229 63 621.6 0 63 230 74 621.6 0 74 231 75 621.6 0 75 232 101 621.6 0 101 233 247 621.6 0 247 234 242 621.6 0 242 235 287 621.6 0 287 236 152 621.6 0 152 237 140 621.6 0 140 238 73 621.6 0 73 239 72 621.6 0 72 240 48 621.6 0 48 241 68 621.6 0 68 242 88 621.6 0 88 243 71 621.6 0 71 244 137 621.6 0 137 245 128 621.6 0 128 246 159 621.6 0 159 247 93 621.6 0 93 248 102 621.6 0 102 249 191 621.6 0 191 250 163 621.6 0 163 251 208 621.6 0 208 252 72 621.6 0 72 253 125 621.6 0 125 254 86 621.6 0 86 255 96 621.6 0 96 256 73 621.6 0 73 257 70 621.6 0 70 258 78 621.6 0 78 259 87 621.6 0 87 260 84 621.6 0 84 261 125 621.6 0 125 262 116 621.6 0 116 263 97 621.6 0 97 264 91 621.6 0 91 265 98 621.6 0 98 266 92 621.6 0 92 267 142 621.6 0 142 268 164 621.6 0 164 269 158 621.6 0 158 270 252 621.6 0 252 271 411 621.6 0 411 272 240 621.6 0 240 273 154 621.6 0 154 274 156 621.6 0 156 275 160 621.6 0 160 276 238 621.6 0 238 277 199 621.6 0 199 278 360 621.6 0 360 279 889 621.6 0 889 280 1646 621.6 0 1646 281 6435 621.6 0 6435 282 3354 621.6 0 3354 283 4233 621.6 0 4233 284 173 621.6 0 173 285 182 621.6 0 182 286 148 621.6 0 148 287 116 621.6 0 116 288 95 621.6 0 95 289 137 621.6 0 137 290 454 621.6 0 454 291 423 621.6 0 423 292 734 621.6 0 734 293 402 621.6 0 402 294 264 621.6 0 264 295 422 621.6 0 422 296 391 621.6 0 391 297 428 621.6 0 428 298 485 621.6 0 485 299 147 621.6 0 147 300 75 621.6 0 75 301 132 621.6 0 132 Finished in 6.26 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 179,604 Reads with adapters: 8,415 (4.7%) Reads that were too short: 184 (0.1%) Reads written (passing filters): 8,231 (4.6%) Total basepairs processed: 32,428,283 bp Total written (filtered): 2,253,111 bp (6.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 8415 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 6 0.0 3 0 0 3 3 16 74 0.0 3 0 56 9 9 17 7899 0.0 3 7290 489 98 22 18 35 0.0 3 7 21 5 2 19 22 0.0 3 1 0 16 5 20 29 0.0 3 1 0 0 28 24 1 0.0 3 0 0 1 27 2 0.0 3 0 0 0 2 31 1 0.0 3 0 0 0 1 32 5 0.0 3 0 0 0 5 37 2 0.0 3 0 0 0 2 41 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 2 0.0 3 0 0 0 2 48 10 0.0 3 0 0 0 10 49 3 0.0 3 0 0 0 3 50 1 0.0 3 0 0 0 1 51 33 0.0 3 0 0 0 33 54 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 1 1 58 3 0.0 3 0 0 0 3 59 3 0.0 3 0 0 0 3 60 6 0.0 3 0 0 0 6 61 1 0.0 3 0 0 0 1 64 3 0.0 3 0 0 0 3 65 4 0.0 3 0 0 0 4 66 1 0.0 3 0 0 0 1 68 3 0.0 3 0 0 0 3 71 2 0.0 3 0 0 0 2 76 2 0.0 3 0 0 0 2 77 3 0.0 3 0 0 0 3 83 2 0.0 3 0 0 0 2 84 1 0.0 3 0 0 0 1 85 27 0.0 3 0 0 0 27 86 18 0.0 3 0 0 0 18 87 3 0.0 3 0 0 0 3 88 6 0.0 3 0 0 1 5 89 5 0.0 3 0 0 0 5 90 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 1 1 92 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 98 3 0.0 3 0 0 0 3 99 3 0.0 3 0 0 0 3 101 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 2 0.0 3 0 0 0 2 109 3 0.0 3 0 0 1 2 110 5 0.0 3 0 0 1 4 111 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 118 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 3 0.0 3 0 0 0 3 133 4 0.0 3 0 0 0 4 134 10 0.0 3 0 0 0 10 135 10 0.0 3 0 0 0 10 136 8 0.0 3 0 0 1 7 138 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 142 4 0.0 3 0 0 0 4 143 3 0.0 3 0 0 1 2 146 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 148 2 0.0 3 0 0 0 2 151 1 0.0 3 0 0 0 1 152 4 0.0 3 0 0 0 4 153 2 0.0 3 0 0 0 2 154 2 0.0 3 0 0 0 2 157 1 0.0 3 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 161 3 0.0 3 0 0 0 3 164 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 172 9 0.0 3 0 0 0 9 173 5 0.0 3 0 0 0 5 174 20 0.0 3 0 0 0 20 175 15 0.0 3 0 0 1 14 177 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 2 0.0 3 0 0 0 2 186 6 0.0 3 0 0 0 6 187 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 201 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 217 6 0.0 3 0 0 1 5 228 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 1 281 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.30 s (36 us/read; 1.65 M reads/minute). === Summary === Total reads processed: 8,231 Reads with adapters: 23 (0.3%) Reads written (passing filters): 8,231 (100.0%) Total basepairs processed: 2,253,111 bp Total written (filtered): 2,253,040 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 23 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 26.1% C: 39.1% G: 21.7% T: 13.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 21 128.6 0 21 4 2 32.2 0 2