This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.11 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 232,214 (9.1%) Reads that were too short: 35,111 (1.4%) Reads written (passing filters): 197,103 (7.7%) Total basepairs processed: 766,381,518 bp Total written (filtered): 55,862,376 bp (7.3%) === Adapter 1 === Sequence: TGTGGC; Type: regular 5'; Length: 6; Trimmed: 232214 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 4408 621.6 0 4408 7 95269 621.6 0 95269 8 2019 621.6 0 2019 9 76519 621.6 0 76519 10 113 621.6 0 113 11 67 621.6 0 67 12 137 621.6 0 137 13 51 621.6 0 51 14 75 621.6 0 75 15 58 621.6 0 58 16 66 621.6 0 66 17 75 621.6 0 75 18 70 621.6 0 70 19 82 621.6 0 82 20 86 621.6 0 86 21 60 621.6 0 60 22 69 621.6 0 69 23 66 621.6 0 66 24 62 621.6 0 62 25 68 621.6 0 68 26 64 621.6 0 64 27 60 621.6 0 60 28 84 621.6 0 84 29 49 621.6 0 49 30 65 621.6 0 65 31 51 621.6 0 51 32 60 621.6 0 60 33 104 621.6 0 104 34 63 621.6 0 63 35 52 621.6 0 52 36 70 621.6 0 70 37 75 621.6 0 75 38 71 621.6 0 71 39 77 621.6 0 77 40 86 621.6 0 86 41 105 621.6 0 105 42 91 621.6 0 91 43 84 621.6 0 84 44 48 621.6 0 48 45 52 621.6 0 52 46 68 621.6 0 68 47 90 621.6 0 90 48 67 621.6 0 67 49 74 621.6 0 74 50 61 621.6 0 61 51 49 621.6 0 49 52 86 621.6 0 86 53 83 621.6 0 83 54 87 621.6 0 87 55 78 621.6 0 78 56 50 621.6 0 50 57 119 621.6 0 119 58 95 621.6 0 95 59 65 621.6 0 65 60 74 621.6 0 74 61 77 621.6 0 77 62 77 621.6 0 77 63 88 621.6 0 88 64 87 621.6 0 87 65 56 621.6 0 56 66 63 621.6 0 63 67 73 621.6 0 73 68 76 621.6 0 76 69 71 621.6 0 71 70 49 621.6 0 49 71 124 621.6 0 124 72 63 621.6 0 63 73 106 621.6 0 106 74 40 621.6 0 40 75 52 621.6 0 52 76 39 621.6 0 39 77 72 621.6 0 72 78 50 621.6 0 50 79 50 621.6 0 50 80 70 621.6 0 70 81 62 621.6 0 62 82 67 621.6 0 67 83 46 621.6 0 46 84 60 621.6 0 60 85 67 621.6 0 67 86 53 621.6 0 53 87 51 621.6 0 51 88 52 621.6 0 52 89 51 621.6 0 51 90 43 621.6 0 43 91 56 621.6 0 56 92 63 621.6 0 63 93 58 621.6 0 58 94 54 621.6 0 54 95 77 621.6 0 77 96 53 621.6 0 53 97 61 621.6 0 61 98 46 621.6 0 46 99 60 621.6 0 60 100 66 621.6 0 66 101 43 621.6 0 43 102 45 621.6 0 45 103 55 621.6 0 55 104 50 621.6 0 50 105 54 621.6 0 54 106 52 621.6 0 52 107 73 621.6 0 73 108 41 621.6 0 41 109 59 621.6 0 59 110 45 621.6 0 45 111 56 621.6 0 56 112 44 621.6 0 44 113 59 621.6 0 59 114 54 621.6 0 54 115 39 621.6 0 39 116 49 621.6 0 49 117 126 621.6 0 126 118 54 621.6 0 54 119 34 621.6 0 34 120 83 621.6 0 83 121 48 621.6 0 48 122 34 621.6 0 34 123 120 621.6 0 120 124 66 621.6 0 66 125 76 621.6 0 76 126 49 621.6 0 49 127 68 621.6 0 68 128 121 621.6 0 121 129 128 621.6 0 128 130 3852 621.6 0 3852 131 1448 621.6 0 1448 132 1870 621.6 0 1870 133 160 621.6 0 160 134 61 621.6 0 61 135 71 621.6 0 71 136 108 621.6 0 108 137 189 621.6 0 189 138 234 621.6 0 234 139 124 621.6 0 124 140 43 621.6 0 43 141 26 621.6 0 26 142 43 621.6 0 43 143 34 621.6 0 34 144 26 621.6 0 26 145 28 621.6 0 28 146 43 621.6 0 43 147 47 621.6 0 47 148 58 621.6 0 58 149 41 621.6 0 41 150 47 621.6 0 47 151 52 621.6 0 52 152 63 621.6 0 63 153 52 621.6 0 52 154 43 621.6 0 43 155 50 621.6 0 50 156 26 621.6 0 26 157 28 621.6 0 28 158 37 621.6 0 37 159 45 621.6 0 45 160 50 621.6 0 50 161 26 621.6 0 26 162 26 621.6 0 26 163 40 621.6 0 40 164 35 621.6 0 35 165 34 621.6 0 34 166 49 621.6 0 49 167 36 621.6 0 36 168 47 621.6 0 47 169 28 621.6 0 28 170 30 621.6 0 30 171 34 621.6 0 34 172 31 621.6 0 31 173 39 621.6 0 39 174 30 621.6 0 30 175 28 621.6 0 28 176 36 621.6 0 36 177 47 621.6 0 47 178 62 621.6 0 62 179 56 621.6 0 56 180 26 621.6 0 26 181 45 621.6 0 45 182 44 621.6 0 44 183 58 621.6 0 58 184 39 621.6 0 39 185 48 621.6 0 48 186 118 621.6 0 118 187 72 621.6 0 72 188 56 621.6 0 56 189 59 621.6 0 59 190 44 621.6 0 44 191 36 621.6 0 36 192 36 621.6 0 36 193 47 621.6 0 47 194 46 621.6 0 46 195 41 621.6 0 41 196 38 621.6 0 38 197 63 621.6 0 63 198 42 621.6 0 42 199 30 621.6 0 30 200 40 621.6 0 40 201 31 621.6 0 31 202 32 621.6 0 32 203 36 621.6 0 36 204 41 621.6 0 41 205 37 621.6 0 37 206 42 621.6 0 42 207 46 621.6 0 46 208 37 621.6 0 37 209 37 621.6 0 37 210 30 621.6 0 30 211 46 621.6 0 46 212 154 621.6 0 154 213 610 621.6 0 610 214 127 621.6 0 127 215 39 621.6 0 39 216 54 621.6 0 54 217 59 621.6 0 59 218 188 621.6 0 188 219 170 621.6 0 170 220 133 621.6 0 133 221 47 621.6 0 47 222 36 621.6 0 36 223 42 621.6 0 42 224 32 621.6 0 32 225 44 621.6 0 44 226 47 621.6 0 47 227 27 621.6 0 27 228 36 621.6 0 36 229 33 621.6 0 33 230 44 621.6 0 44 231 33 621.6 0 33 232 66 621.6 0 66 233 46 621.6 0 46 234 56 621.6 0 56 235 80 621.6 0 80 236 112 621.6 0 112 237 106 621.6 0 106 238 119 621.6 0 119 239 70 621.6 0 70 240 36 621.6 0 36 241 71 621.6 0 71 242 58 621.6 0 58 243 235 621.6 0 235 244 214 621.6 0 214 245 243 621.6 0 243 246 111 621.6 0 111 247 96 621.6 0 96 248 75 621.6 0 75 249 67 621.6 0 67 250 71 621.6 0 71 251 51 621.6 0 51 252 49 621.6 0 49 253 36 621.6 0 36 254 61 621.6 0 61 255 70 621.6 0 70 256 219 621.6 0 219 257 174 621.6 0 174 258 174 621.6 0 174 259 141 621.6 0 141 260 983 621.6 0 983 261 754 621.6 0 754 262 760 621.6 0 760 263 150 621.6 0 150 264 62 621.6 0 62 265 67 621.6 0 67 266 54 621.6 0 54 267 64 621.6 0 64 268 69 621.6 0 69 269 60 621.6 0 60 270 71 621.6 0 71 271 75 621.6 0 75 272 130 621.6 0 130 273 234 621.6 0 234 274 93 621.6 0 93 275 118 621.6 0 118 276 202 621.6 0 202 277 103 621.6 0 103 278 125 621.6 0 125 279 199 621.6 0 199 280 226 621.6 0 226 281 216 621.6 0 216 282 224 621.6 0 224 283 266 621.6 0 266 284 367 621.6 0 367 285 439 621.6 0 439 286 7002 621.6 0 7002 287 6402 621.6 0 6402 288 9142 621.6 0 9142 289 101 621.6 0 101 290 55 621.6 0 55 291 35 621.6 0 35 292 45 621.6 0 45 293 54 621.6 0 54 294 54 621.6 0 54 295 51 621.6 0 51 296 126 621.6 0 126 297 136 621.6 0 136 298 283 621.6 0 283 299 178 621.6 0 178 300 59 621.6 0 59 301 31 621.6 0 31 Finished in 9.71 s (49 us/read; 1.22 M reads/minute). === Summary === Total reads processed: 197,103 Reads with adapters: 95,111 (48.3%) Reads that were too short: 22 (0.0%) Reads written (passing filters): 95,089 (48.2%) Total basepairs processed: 55,862,376 bp Total written (filtered): 25,975,209 bp (46.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 95111 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 26 0.0 3 0 0 24 2 19 917 0.0 3 0 901 15 1 20 93152 0.0 4 91724 1379 38 7 4 21 169 0.0 4 23 141 4 1 22 17 0.0 4 5 0 12 23 40 0.0 4 1 0 0 36 3 24 3 0.0 4 0 0 0 1 2 27 1 0.0 4 1 79 1 0.0 4 0 0 0 1 84 1 0.0 4 0 0 0 1 103 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 125 2 0.0 4 0 0 0 0 2 126 5 0.0 4 0 0 0 0 5 127 683 0.0 4 0 0 0 0 683 128 2 0.0 4 0 0 0 0 2 129 66 0.0 4 0 0 0 0 66 139 3 0.0 4 1 0 0 0 2 153 1 0.0 4 0 0 0 0 1 155 1 0.0 4 1 196 1 0.0 4 1 215 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 228 5 0.0 4 0 0 0 0 5 229 3 0.0 4 0 0 0 0 3 233 2 0.0 4 0 0 1 0 1 235 1 0.0 4 0 0 0 0 1 237 1 0.0 4 0 0 0 0 1 238 1 0.0 4 0 0 0 0 1 265 1 0.0 4 0 0 0 0 1 288 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.28 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 95,089 Reads with adapters: 2,137 (2.2%) Reads written (passing filters): 95,089 (100.0%) Total basepairs processed: 25,975,209 bp Total written (filtered): 25,949,743 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2137 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 7.3% C: 5.6% G: 85.2% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 159 1485.8 0 159 4 22 371.4 0 22 5 9 92.9 0 9 6 1 23.2 0 1 9 6 0.4 0 0 6 10 40 0.1 1 1 39 11 1752 0.0 1 7 1745 12 107 0.0 1 10 97 13 4 0.0 1 1 3 15 3 0.0 1 0 3 16 1 0.0 1 0 1 20 3 0.0 1 1 2 21 2 0.0 1 2 24 7 0.0 1 6 1 35 1 0.0 1 1 40 1 0.0 1 0 1 114 2 0.0 1 2 152 2 0.0 1 0 2 163 1 0.0 1 1 167 1 0.0 1 1 171 6 0.0 1 6 179 1 0.0 1 1 202 1 0.0 1 1 210 2 0.0 1 1 1 225 2 0.0 1 2 243 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.