This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.70 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 91,410 (3.6%) Reads that were too short: 29,420 (1.2%) Reads written (passing filters): 61,990 (2.4%) Total basepairs processed: 766,381,518 bp Total written (filtered): 14,974,305 bp (2.0%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 91410 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 318 621.6 0 318 7 928 621.6 0 928 8 16859 621.6 0 16859 9 14482 621.6 0 14482 10 105 621.6 0 105 11 105 621.6 0 105 12 93 621.6 0 93 13 81 621.6 0 81 14 90 621.6 0 90 15 88 621.6 0 88 16 111 621.6 0 111 17 82 621.6 0 82 18 78 621.6 0 78 19 66 621.6 0 66 20 88 621.6 0 88 21 104 621.6 0 104 22 94 621.6 0 94 23 103 621.6 0 103 24 85 621.6 0 85 25 104 621.6 0 104 26 82 621.6 0 82 27 72 621.6 0 72 28 109 621.6 0 109 29 90 621.6 0 90 30 87 621.6 0 87 31 79 621.6 0 79 32 101 621.6 0 101 33 84 621.6 0 84 34 97 621.6 0 97 35 83 621.6 0 83 36 93 621.6 0 93 37 98 621.6 0 98 38 193 621.6 0 193 39 106 621.6 0 106 40 161 621.6 0 161 41 103 621.6 0 103 42 95 621.6 0 95 43 87 621.6 0 87 44 68 621.6 0 68 45 78 621.6 0 78 46 99 621.6 0 99 47 90 621.6 0 90 48 103 621.6 0 103 49 109 621.6 0 109 50 86 621.6 0 86 51 107 621.6 0 107 52 126 621.6 0 126 53 115 621.6 0 115 54 104 621.6 0 104 55 96 621.6 0 96 56 100 621.6 0 100 57 94 621.6 0 94 58 109 621.6 0 109 59 70 621.6 0 70 60 123 621.6 0 123 61 111 621.6 0 111 62 124 621.6 0 124 63 120 621.6 0 120 64 106 621.6 0 106 65 109 621.6 0 109 66 134 621.6 0 134 67 136 621.6 0 136 68 128 621.6 0 128 69 111 621.6 0 111 70 111 621.6 0 111 71 127 621.6 0 127 72 114 621.6 0 114 73 88 621.6 0 88 74 131 621.6 0 131 75 136 621.6 0 136 76 121 621.6 0 121 77 110 621.6 0 110 78 159 621.6 0 159 79 100 621.6 0 100 80 125 621.6 0 125 81 92 621.6 0 92 82 128 621.6 0 128 83 115 621.6 0 115 84 137 621.6 0 137 85 102 621.6 0 102 86 92 621.6 0 92 87 127 621.6 0 127 88 126 621.6 0 126 89 95 621.6 0 95 90 121 621.6 0 121 91 193 621.6 0 193 92 120 621.6 0 120 93 144 621.6 0 144 94 139 621.6 0 139 95 211 621.6 0 211 96 186 621.6 0 186 97 769 621.6 0 769 98 1126 621.6 0 1126 99 963 621.6 0 963 100 401 621.6 0 401 101 103 621.6 0 103 102 80 621.6 0 80 103 94 621.6 0 94 104 100 621.6 0 100 105 116 621.6 0 116 106 124 621.6 0 124 107 138 621.6 0 138 108 159 621.6 0 159 109 186 621.6 0 186 110 151 621.6 0 151 111 557 621.6 0 557 112 211 621.6 0 211 113 453 621.6 0 453 114 86 621.6 0 86 115 109 621.6 0 109 116 78 621.6 0 78 117 110 621.6 0 110 118 103 621.6 0 103 119 116 621.6 0 116 120 111 621.6 0 111 121 92 621.6 0 92 122 119 621.6 0 119 123 89 621.6 0 89 124 90 621.6 0 90 125 89 621.6 0 89 126 113 621.6 0 113 127 97 621.6 0 97 128 166 621.6 0 166 129 139 621.6 0 139 130 163 621.6 0 163 131 147 621.6 0 147 132 353 621.6 0 353 133 167 621.6 0 167 134 251 621.6 0 251 135 116 621.6 0 116 136 135 621.6 0 135 137 95 621.6 0 95 138 117 621.6 0 117 139 84 621.6 0 84 140 112 621.6 0 112 141 151 621.6 0 151 142 103 621.6 0 103 143 92 621.6 0 92 144 109 621.6 0 109 145 122 621.6 0 122 146 147 621.6 0 147 147 152 621.6 0 152 148 131 621.6 0 131 149 117 621.6 0 117 150 103 621.6 0 103 151 93 621.6 0 93 152 114 621.6 0 114 153 146 621.6 0 146 154 442 621.6 0 442 155 544 621.6 0 544 156 624 621.6 0 624 157 197 621.6 0 197 158 107 621.6 0 107 159 105 621.6 0 105 160 91 621.6 0 91 161 147 621.6 0 147 162 110 621.6 0 110 163 90 621.6 0 90 164 113 621.6 0 113 165 145 621.6 0 145 166 678 621.6 0 678 167 254 621.6 0 254 168 267 621.6 0 267 169 91 621.6 0 91 170 126 621.6 0 126 171 91 621.6 0 91 172 109 621.6 0 109 173 93 621.6 0 93 174 102 621.6 0 102 175 106 621.6 0 106 176 115 621.6 0 115 177 111 621.6 0 111 178 121 621.6 0 121 179 131 621.6 0 131 180 157 621.6 0 157 181 196 621.6 0 196 182 106 621.6 0 106 183 108 621.6 0 108 184 120 621.6 0 120 185 132 621.6 0 132 186 119 621.6 0 119 187 106 621.6 0 106 188 127 621.6 0 127 189 120 621.6 0 120 190 105 621.6 0 105 191 93 621.6 0 93 192 128 621.6 0 128 193 380 621.6 0 380 194 310 621.6 0 310 195 293 621.6 0 293 196 353 621.6 0 353 197 151 621.6 0 151 198 161 621.6 0 161 199 168 621.6 0 168 200 367 621.6 0 367 201 232 621.6 0 232 202 261 621.6 0 261 203 99 621.6 0 99 204 103 621.6 0 103 205 115 621.6 0 115 206 136 621.6 0 136 207 135 621.6 0 135 208 108 621.6 0 108 209 94 621.6 0 94 210 90 621.6 0 90 211 86 621.6 0 86 212 109 621.6 0 109 213 95 621.6 0 95 214 130 621.6 0 130 215 132 621.6 0 132 216 132 621.6 0 132 217 180 621.6 0 180 218 140 621.6 0 140 219 142 621.6 0 142 220 629 621.6 0 629 221 607 621.6 0 607 222 595 621.6 0 595 223 221 621.6 0 221 224 122 621.6 0 122 225 98 621.6 0 98 226 109 621.6 0 109 227 136 621.6 0 136 228 116 621.6 0 116 229 119 621.6 0 119 230 103 621.6 0 103 231 123 621.6 0 123 232 115 621.6 0 115 233 106 621.6 0 106 234 92 621.6 0 92 235 136 621.6 0 136 236 152 621.6 0 152 237 345 621.6 0 345 238 609 621.6 0 609 239 432 621.6 0 432 240 229 621.6 0 229 241 226 621.6 0 226 242 255 621.6 0 255 243 586 621.6 0 586 244 425 621.6 0 425 245 443 621.6 0 443 246 199 621.6 0 199 247 126 621.6 0 126 248 167 621.6 0 167 249 193 621.6 0 193 250 142 621.6 0 142 251 174 621.6 0 174 252 184 621.6 0 184 253 201 621.6 0 201 254 1025 621.6 0 1025 255 602 621.6 0 602 256 1042 621.6 0 1042 257 143 621.6 0 143 258 161 621.6 0 161 259 198 621.6 0 198 260 233 621.6 0 233 261 378 621.6 0 378 262 4165 621.6 0 4165 263 1628 621.6 0 1628 264 2378 621.6 0 2378 265 213 621.6 0 213 266 116 621.6 0 116 267 98 621.6 0 98 268 103 621.6 0 103 269 128 621.6 0 128 270 154 621.6 0 154 271 146 621.6 0 146 272 118 621.6 0 118 273 114 621.6 0 114 274 344 621.6 0 344 275 294 621.6 0 294 276 254 621.6 0 254 277 134 621.6 0 134 278 129 621.6 0 129 279 175 621.6 0 175 280 189 621.6 0 189 281 252 621.6 0 252 282 365 621.6 0 365 283 267 621.6 0 267 284 153 621.6 0 153 285 149 621.6 0 149 286 122 621.6 0 122 287 122 621.6 0 122 288 160 621.6 0 160 289 246 621.6 0 246 290 192 621.6 0 192 291 192 621.6 0 192 292 141 621.6 0 141 293 126 621.6 0 126 294 172 621.6 0 172 295 457 621.6 0 457 296 142 621.6 0 142 297 136 621.6 0 136 298 101 621.6 0 101 299 129 621.6 0 129 300 173 621.6 0 173 301 159 621.6 0 159 Finished in 2.80 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 61,990 Reads with adapters: 17,112 (27.6%) Reads that were too short: 88 (0.1%) Reads written (passing filters): 17,024 (27.5%) Total basepairs processed: 14,974,305 bp Total written (filtered): 4,686,769 bp (31.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 17112 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 6 0.0 2 0 0 0 6 15 14 0.0 3 0 0 10 4 16 245 0.0 3 0 223 16 6 17 16528 0.0 3 15327 986 176 39 18 51 0.0 3 2 37 4 8 19 73 0.0 3 4 3 34 32 20 27 0.0 3 2 0 0 25 22 2 0.0 3 0 0 0 2 24 1 0.0 3 0 0 0 1 26 1 0.0 3 0 1 31 1 0.0 3 0 0 0 1 33 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 37 10 0.0 3 0 0 0 10 40 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 1 52 3 0.0 3 0 0 0 3 55 2 0.0 3 0 0 0 2 59 3 0.0 3 0 0 0 3 60 3 0.0 3 0 0 0 3 63 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 67 1 0.0 3 0 0 0 1 73 2 0.0 3 0 0 0 2 74 4 0.0 3 0 0 0 4 75 1 0.0 3 0 0 0 1 76 15 0.0 3 0 0 0 15 77 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 80 5 0.0 3 0 0 0 5 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 3 0.0 3 0 0 0 3 90 1 0.0 3 0 0 0 1 93 7 0.0 3 0 0 0 7 94 4 0.0 3 0 0 0 4 96 2 0.0 3 0 0 0 2 97 1 0.0 3 0 0 0 1 98 3 0.0 3 0 0 0 3 100 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 1 102 2 0.0 3 0 0 0 2 103 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 116 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 128 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 138 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 148 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 154 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 161 4 0.0 3 0 0 1 3 164 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 187 1 0.0 3 0 0 0 1 188 23 0.0 3 0 0 0 23 190 1 0.0 3 0 0 0 1 194 2 0.0 3 0 0 0 2 195 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 222 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 281 3 0.0 3 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.60 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 17,024 Reads with adapters: 92 (0.5%) Reads written (passing filters): 17,024 (100.0%) Total basepairs processed: 4,686,769 bp Total written (filtered): 4,686,443 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 92 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 31.5% C: 8.7% G: 53.3% T: 6.5% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 44 266.0 0 44 4 46 66.5 0 46 5 2 16.6 0 2