This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.38 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 68,090 (2.7%) Reads that were too short: 18,040 (0.7%) Reads written (passing filters): 50,050 (2.0%) Total basepairs processed: 766,381,518 bp Total written (filtered): 12,948,868 bp (1.7%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 68090 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 228 621.6 0 228 7 708 621.6 0 708 8 15655 621.6 0 15655 9 17441 621.6 0 17441 10 79 621.6 0 79 11 51 621.6 0 51 12 39 621.6 0 39 13 65 621.6 0 65 14 51 621.6 0 51 15 49 621.6 0 49 16 44 621.6 0 44 17 38 621.6 0 38 18 36 621.6 0 36 19 46 621.6 0 46 20 59 621.6 0 59 21 57 621.6 0 57 22 63 621.6 0 63 23 60 621.6 0 60 24 60 621.6 0 60 25 52 621.6 0 52 26 53 621.6 0 53 27 42 621.6 0 42 28 64 621.6 0 64 29 54 621.6 0 54 30 51 621.6 0 51 31 63 621.6 0 63 32 66 621.6 0 66 33 52 621.6 0 52 34 67 621.6 0 67 35 49 621.6 0 49 36 61 621.6 0 61 37 66 621.6 0 66 38 135 621.6 0 135 39 69 621.6 0 69 40 83 621.6 0 83 41 70 621.6 0 70 42 54 621.6 0 54 43 57 621.6 0 57 44 53 621.6 0 53 45 46 621.6 0 46 46 54 621.6 0 54 47 47 621.6 0 47 48 60 621.6 0 60 49 48 621.6 0 48 50 51 621.6 0 51 51 73 621.6 0 73 52 64 621.6 0 64 53 57 621.6 0 57 54 65 621.6 0 65 55 55 621.6 0 55 56 71 621.6 0 71 57 42 621.6 0 42 58 55 621.6 0 55 59 43 621.6 0 43 60 91 621.6 0 91 61 70 621.6 0 70 62 55 621.6 0 55 63 80 621.6 0 80 64 73 621.6 0 73 65 61 621.6 0 61 66 81 621.6 0 81 67 75 621.6 0 75 68 63 621.6 0 63 69 68 621.6 0 68 70 67 621.6 0 67 71 78 621.6 0 78 72 71 621.6 0 71 73 74 621.6 0 74 74 89 621.6 0 89 75 81 621.6 0 81 76 45 621.6 0 45 77 60 621.6 0 60 78 82 621.6 0 82 79 42 621.6 0 42 80 56 621.6 0 56 81 73 621.6 0 73 82 80 621.6 0 80 83 55 621.6 0 55 84 90 621.6 0 90 85 80 621.6 0 80 86 72 621.6 0 72 87 63 621.6 0 63 88 81 621.6 0 81 89 81 621.6 0 81 90 39 621.6 0 39 91 130 621.6 0 130 92 76 621.6 0 76 93 77 621.6 0 77 94 74 621.6 0 74 95 149 621.6 0 149 96 90 621.6 0 90 97 283 621.6 0 283 98 358 621.6 0 358 99 285 621.6 0 285 100 160 621.6 0 160 101 66 621.6 0 66 102 52 621.6 0 52 103 58 621.6 0 58 104 76 621.6 0 76 105 79 621.6 0 79 106 63 621.6 0 63 107 77 621.6 0 77 108 79 621.6 0 79 109 91 621.6 0 91 110 87 621.6 0 87 111 494 621.6 0 494 112 175 621.6 0 175 113 283 621.6 0 283 114 63 621.6 0 63 115 65 621.6 0 65 116 47 621.6 0 47 117 58 621.6 0 58 118 72 621.6 0 72 119 82 621.6 0 82 120 75 621.6 0 75 121 55 621.6 0 55 122 78 621.6 0 78 123 59 621.6 0 59 124 66 621.6 0 66 125 74 621.6 0 74 126 58 621.6 0 58 127 74 621.6 0 74 128 108 621.6 0 108 129 84 621.6 0 84 130 75 621.6 0 75 131 76 621.6 0 76 132 134 621.6 0 134 133 68 621.6 0 68 134 102 621.6 0 102 135 69 621.6 0 69 136 80 621.6 0 80 137 71 621.6 0 71 138 70 621.6 0 70 139 52 621.6 0 52 140 62 621.6 0 62 141 83 621.6 0 83 142 66 621.6 0 66 143 57 621.6 0 57 144 57 621.6 0 57 145 79 621.6 0 79 146 76 621.6 0 76 147 58 621.6 0 58 148 77 621.6 0 77 149 63 621.6 0 63 150 66 621.6 0 66 151 66 621.6 0 66 152 75 621.6 0 75 153 84 621.6 0 84 154 195 621.6 0 195 155 249 621.6 0 249 156 220 621.6 0 220 157 121 621.6 0 121 158 64 621.6 0 64 159 60 621.6 0 60 160 55 621.6 0 55 161 118 621.6 0 118 162 56 621.6 0 56 163 52 621.6 0 52 164 104 621.6 0 104 165 69 621.6 0 69 166 238 621.6 0 238 167 91 621.6 0 91 168 110 621.6 0 110 169 65 621.6 0 65 170 61 621.6 0 61 171 46 621.6 0 46 172 66 621.6 0 66 173 52 621.6 0 52 174 77 621.6 0 77 175 61 621.6 0 61 176 65 621.6 0 65 177 69 621.6 0 69 178 64 621.6 0 64 179 87 621.6 0 87 180 122 621.6 0 122 181 135 621.6 0 135 182 72 621.6 0 72 183 61 621.6 0 61 184 68 621.6 0 68 185 55 621.6 0 55 186 75 621.6 0 75 187 68 621.6 0 68 188 54 621.6 0 54 189 71 621.6 0 71 190 70 621.6 0 70 191 70 621.6 0 70 192 57 621.6 0 57 193 140 621.6 0 140 194 221 621.6 0 221 195 123 621.6 0 123 196 246 621.6 0 246 197 67 621.6 0 67 198 82 621.6 0 82 199 92 621.6 0 92 200 131 621.6 0 131 201 112 621.6 0 112 202 107 621.6 0 107 203 53 621.6 0 53 204 54 621.6 0 54 205 51 621.6 0 51 206 76 621.6 0 76 207 84 621.6 0 84 208 78 621.6 0 78 209 64 621.6 0 64 210 74 621.6 0 74 211 59 621.6 0 59 212 61 621.6 0 61 213 83 621.6 0 83 214 86 621.6 0 86 215 66 621.6 0 66 216 66 621.6 0 66 217 81 621.6 0 81 218 73 621.6 0 73 219 91 621.6 0 91 220 370 621.6 0 370 221 259 621.6 0 259 222 243 621.6 0 243 223 120 621.6 0 120 224 83 621.6 0 83 225 63 621.6 0 63 226 66 621.6 0 66 227 83 621.6 0 83 228 76 621.6 0 76 229 84 621.6 0 84 230 76 621.6 0 76 231 61 621.6 0 61 232 62 621.6 0 62 233 60 621.6 0 60 234 75 621.6 0 75 235 57 621.6 0 57 236 89 621.6 0 89 237 316 621.6 0 316 238 614 621.6 0 614 239 366 621.6 0 366 240 84 621.6 0 84 241 139 621.6 0 139 242 117 621.6 0 117 243 318 621.6 0 318 244 191 621.6 0 191 245 199 621.6 0 199 246 98 621.6 0 98 247 77 621.6 0 77 248 83 621.6 0 83 249 139 621.6 0 139 250 100 621.6 0 100 251 105 621.6 0 105 252 117 621.6 0 117 253 120 621.6 0 120 254 344 621.6 0 344 255 195 621.6 0 195 256 416 621.6 0 416 257 106 621.6 0 106 258 110 621.6 0 110 259 112 621.6 0 112 260 137 621.6 0 137 261 254 621.6 0 254 262 3092 621.6 0 3092 263 1019 621.6 0 1019 264 1813 621.6 0 1813 265 128 621.6 0 128 266 72 621.6 0 72 267 63 621.6 0 63 268 65 621.6 0 65 269 78 621.6 0 78 270 79 621.6 0 79 271 75 621.6 0 75 272 79 621.6 0 79 273 79 621.6 0 79 274 151 621.6 0 151 275 113 621.6 0 113 276 146 621.6 0 146 277 84 621.6 0 84 278 57 621.6 0 57 279 97 621.6 0 97 280 105 621.6 0 105 281 116 621.6 0 116 282 135 621.6 0 135 283 111 621.6 0 111 284 89 621.6 0 89 285 76 621.6 0 76 286 99 621.6 0 99 287 76 621.6 0 76 288 89 621.6 0 89 289 145 621.6 0 145 290 108 621.6 0 108 291 108 621.6 0 108 292 92 621.6 0 92 293 119 621.6 0 119 294 126 621.6 0 126 295 574 621.6 0 574 296 81 621.6 0 81 297 71 621.6 0 71 298 69 621.6 0 69 299 86 621.6 0 86 300 102 621.6 0 102 301 82 621.6 0 82 Finished in 2.56 s (51 us/read; 1.17 M reads/minute). === Summary === Total reads processed: 50,050 Reads with adapters: 18,380 (36.7%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 18,374 (36.7%) Total basepairs processed: 12,948,868 bp Total written (filtered): 4,991,453 bp (38.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 18380 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 10 0.0 3 0 0 9 1 19 275 0.0 3 0 270 5 20 17949 0.0 4 17689 251 6 3 21 45 0.0 4 4 41 22 1 0.0 4 0 0 1 23 20 0.0 4 2 0 0 18 35 2 0.0 4 2 95 1 0.0 4 0 0 0 1 121 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 70 0.0 4 0 0 0 0 70 136 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 257 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.65 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 18,374 Reads with adapters: 2,679 (14.6%) Reads written (passing filters): 18,374 (100.0%) Total basepairs processed: 4,991,453 bp Total written (filtered): 4,964,553 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2679 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.7% C: 3.3% G: 94.7% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 287.1 0 1 4 4 71.8 0 4 9 1291 0.1 0 39 1252 10 1153 0.0 1 62 1091 11 211 0.0 1 1 210 12 4 0.0 1 0 4 13 8 0.0 1 0 8 46 1 0.0 1 1 152 1 0.0 1 0 1 169 1 0.0 1 1 223 4 0.0 1 3 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.