This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCCTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 69.45 s (27 us/read; 2.20 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 221,205 (8.7%) Reads that were too short: 21,087 (0.8%) Reads written (passing filters): 200,118 (7.9%) Total basepairs processed: 766,381,518 bp Total written (filtered): 45,341,154 bp (5.9%) === Adapter 1 === Sequence: AGCCTC; Type: regular 5'; Length: 6; Trimmed: 221205 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 557 621.6 0 557 7 6472 621.6 0 6472 8 5405 621.6 0 5405 9 139 621.6 0 139 10 139 621.6 0 139 11 224 621.6 0 224 12 223 621.6 0 223 13 152 621.6 0 152 14 129 621.6 0 129 15 122 621.6 0 122 16 135 621.6 0 135 17 123 621.6 0 123 18 148 621.6 0 148 19 173 621.6 0 173 20 132 621.6 0 132 21 149 621.6 0 149 22 153 621.6 0 153 23 147 621.6 0 147 24 148 621.6 0 148 25 152 621.6 0 152 26 138 621.6 0 138 27 127 621.6 0 127 28 152 621.6 0 152 29 305 621.6 0 305 30 121 621.6 0 121 31 116 621.6 0 116 32 102 621.6 0 102 33 121 621.6 0 121 34 110 621.6 0 110 35 130 621.6 0 130 36 155 621.6 0 155 37 152 621.6 0 152 38 220 621.6 0 220 39 434 621.6 0 434 40 174 621.6 0 174 41 221 621.6 0 221 42 172 621.6 0 172 43 228 621.6 0 228 44 369 621.6 0 369 45 211 621.6 0 211 46 222 621.6 0 222 47 163 621.6 0 163 48 185 621.6 0 185 49 322 621.6 0 322 50 358 621.6 0 358 51 592 621.6 0 592 52 784 621.6 0 784 53 1002 621.6 0 1002 54 2731 621.6 0 2731 55 6539 621.6 0 6539 56 69180 621.6 0 69180 57 21087 621.6 0 21087 58 31595 621.6 0 31595 59 239 621.6 0 239 60 139 621.6 0 139 61 167 621.6 0 167 62 417 621.6 0 417 63 192 621.6 0 192 64 391 621.6 0 391 65 112 621.6 0 112 66 136 621.6 0 136 67 141 621.6 0 141 68 116 621.6 0 116 69 151 621.6 0 151 70 209 621.6 0 209 71 130 621.6 0 130 72 146 621.6 0 146 73 131 621.6 0 131 74 146 621.6 0 146 75 123 621.6 0 123 76 114 621.6 0 114 77 110 621.6 0 110 78 77 621.6 0 77 79 100 621.6 0 100 80 126 621.6 0 126 81 137 621.6 0 137 82 125 621.6 0 125 83 219 621.6 0 219 84 152 621.6 0 152 85 177 621.6 0 177 86 156 621.6 0 156 87 141 621.6 0 141 88 220 621.6 0 220 89 163 621.6 0 163 90 128 621.6 0 128 91 86 621.6 0 86 92 100 621.6 0 100 93 92 621.6 0 92 94 91 621.6 0 91 95 108 621.6 0 108 96 106 621.6 0 106 97 93 621.6 0 93 98 114 621.6 0 114 99 90 621.6 0 90 100 115 621.6 0 115 101 141 621.6 0 141 102 81 621.6 0 81 103 235 621.6 0 235 104 265 621.6 0 265 105 267 621.6 0 267 106 93 621.6 0 93 107 80 621.6 0 80 108 117 621.6 0 117 109 107 621.6 0 107 110 105 621.6 0 105 111 120 621.6 0 120 112 88 621.6 0 88 113 129 621.6 0 129 114 119 621.6 0 119 115 120 621.6 0 120 116 87 621.6 0 87 117 116 621.6 0 116 118 123 621.6 0 123 119 115 621.6 0 115 120 125 621.6 0 125 121 141 621.6 0 141 122 86 621.6 0 86 123 144 621.6 0 144 124 519 621.6 0 519 125 1421 621.6 0 1421 126 1046 621.6 0 1046 127 150 621.6 0 150 128 125 621.6 0 125 129 143 621.6 0 143 130 179 621.6 0 179 131 150 621.6 0 150 132 147 621.6 0 147 133 143 621.6 0 143 134 110 621.6 0 110 135 158 621.6 0 158 136 89 621.6 0 89 137 102 621.6 0 102 138 130 621.6 0 130 139 136 621.6 0 136 140 183 621.6 0 183 141 163 621.6 0 163 142 171 621.6 0 171 143 166 621.6 0 166 144 160 621.6 0 160 145 229 621.6 0 229 146 358 621.6 0 358 147 1721 621.6 0 1721 148 1243 621.6 0 1243 149 2051 621.6 0 2051 150 892 621.6 0 892 151 356 621.6 0 356 152 504 621.6 0 504 153 178 621.6 0 178 154 176 621.6 0 176 155 133 621.6 0 133 156 130 621.6 0 130 157 129 621.6 0 129 158 147 621.6 0 147 159 126 621.6 0 126 160 404 621.6 0 404 161 347 621.6 0 347 162 1346 621.6 0 1346 163 158 621.6 0 158 164 144 621.6 0 144 165 202 621.6 0 202 166 429 621.6 0 429 167 2278 621.6 0 2278 168 2108 621.6 0 2108 169 1978 621.6 0 1978 170 197 621.6 0 197 171 230 621.6 0 230 172 345 621.6 0 345 173 324 621.6 0 324 174 210 621.6 0 210 175 656 621.6 0 656 176 439 621.6 0 439 177 723 621.6 0 723 178 219 621.6 0 219 179 90 621.6 0 90 180 188 621.6 0 188 181 146 621.6 0 146 182 142 621.6 0 142 183 165 621.6 0 165 184 97 621.6 0 97 185 134 621.6 0 134 186 147 621.6 0 147 187 122 621.6 0 122 188 171 621.6 0 171 189 127 621.6 0 127 190 150 621.6 0 150 191 108 621.6 0 108 192 196 621.6 0 196 193 235 621.6 0 235 194 294 621.6 0 294 195 1143 621.6 0 1143 196 1116 621.6 0 1116 197 719 621.6 0 719 198 400 621.6 0 400 199 466 621.6 0 466 200 2905 621.6 0 2905 201 1386 621.6 0 1386 202 1659 621.6 0 1659 203 172 621.6 0 172 204 104 621.6 0 104 205 147 621.6 0 147 206 140 621.6 0 140 207 109 621.6 0 109 208 118 621.6 0 118 209 89 621.6 0 89 210 75 621.6 0 75 211 105 621.6 0 105 212 92 621.6 0 92 213 90 621.6 0 90 214 109 621.6 0 109 215 118 621.6 0 118 216 103 621.6 0 103 217 108 621.6 0 108 218 140 621.6 0 140 219 232 621.6 0 232 220 296 621.6 0 296 221 351 621.6 0 351 222 126 621.6 0 126 223 113 621.6 0 113 224 97 621.6 0 97 225 108 621.6 0 108 226 127 621.6 0 127 227 108 621.6 0 108 228 159 621.6 0 159 229 249 621.6 0 249 230 387 621.6 0 387 231 542 621.6 0 542 232 731 621.6 0 731 233 398 621.6 0 398 234 525 621.6 0 525 235 107 621.6 0 107 236 84 621.6 0 84 237 97 621.6 0 97 238 100 621.6 0 100 239 155 621.6 0 155 240 186 621.6 0 186 241 178 621.6 0 178 242 151 621.6 0 151 243 157 621.6 0 157 244 177 621.6 0 177 245 97 621.6 0 97 246 164 621.6 0 164 247 139 621.6 0 139 248 155 621.6 0 155 249 137 621.6 0 137 250 135 621.6 0 135 251 126 621.6 0 126 252 100 621.6 0 100 253 93 621.6 0 93 254 111 621.6 0 111 255 113 621.6 0 113 256 119 621.6 0 119 257 105 621.6 0 105 258 141 621.6 0 141 259 118 621.6 0 118 260 112 621.6 0 112 261 132 621.6 0 132 262 127 621.6 0 127 263 114 621.6 0 114 264 111 621.6 0 111 265 145 621.6 0 145 266 120 621.6 0 120 267 151 621.6 0 151 268 373 621.6 0 373 269 344 621.6 0 344 270 417 621.6 0 417 271 201 621.6 0 201 272 107 621.6 0 107 273 165 621.6 0 165 274 154 621.6 0 154 275 195 621.6 0 195 276 240 621.6 0 240 277 161 621.6 0 161 278 289 621.6 0 289 279 214 621.6 0 214 280 247 621.6 0 247 281 468 621.6 0 468 282 427 621.6 0 427 283 666 621.6 0 666 284 116 621.6 0 116 285 88 621.6 0 88 286 85 621.6 0 85 287 166 621.6 0 166 288 179 621.6 0 179 289 402 621.6 0 402 290 180 621.6 0 180 291 182 621.6 0 182 292 213 621.6 0 213 293 317 621.6 0 317 294 354 621.6 0 354 295 278 621.6 0 278 296 153 621.6 0 153 297 139 621.6 0 139 298 109 621.6 0 109 299 126 621.6 0 126 300 538 621.6 0 538 301 510 621.6 0 510 Finished in 7.79 s (39 us/read; 1.54 M reads/minute). === Summary === Total reads processed: 200,118 Reads with adapters: 6,972 (3.5%) Reads that were too short: 257 (0.1%) Reads written (passing filters): 6,715 (3.4%) Total basepairs processed: 45,341,154 bp Total written (filtered): 1,815,662 bp (4.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6972 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 3 0.0 3 0 0 0 3 16 66 0.0 3 0 51 8 7 17 6315 0.0 3 5893 333 61 28 18 17 0.0 3 1 13 2 1 19 13 0.0 3 1 0 5 7 20 40 0.0 3 0 0 1 39 22 6 0.0 3 0 0 0 6 23 3 0.0 3 0 0 0 3 24 1 0.0 3 1 25 3 0.0 3 0 0 0 3 28 3 0.0 3 0 0 0 3 29 3 0.0 3 0 0 0 3 32 2 0.0 3 0 0 0 2 33 1 0.0 3 0 0 0 1 34 2 0.0 3 0 0 0 2 37 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 44 3 0.0 3 0 0 0 3 46 1 0.0 3 0 0 0 1 47 8 0.0 3 0 0 1 7 49 5 0.0 3 0 0 0 5 50 2 0.0 3 0 0 0 2 51 5 0.0 3 0 0 0 5 52 2 0.0 3 0 0 0 2 53 76 0.0 3 0 0 0 76 56 11 0.0 3 0 0 0 11 58 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 64 1 0.0 3 0 0 0 1 65 3 0.0 3 0 0 0 3 66 4 0.0 3 0 0 0 4 67 7 0.0 3 0 0 1 6 68 14 0.0 3 0 0 0 14 69 4 0.0 3 0 0 0 4 71 4 0.0 3 0 0 0 4 72 2 0.0 3 0 0 0 2 75 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 83 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 92 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 98 4 0.0 3 0 0 0 4 101 1 0.0 3 0 0 0 1 104 3 0.0 3 0 0 0 3 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 108 3 0.0 3 0 0 0 3 111 5 0.0 3 0 0 0 5 112 2 0.0 3 0 0 0 2 113 2 0.0 3 0 0 0 2 115 2 0.0 3 0 0 0 2 116 20 0.0 3 0 0 0 20 117 3 0.0 3 0 0 0 3 119 5 0.0 3 0 0 1 4 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 125 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 134 4 0.0 3 0 0 0 4 136 19 0.0 3 0 0 0 19 137 2 0.0 3 0 0 1 1 138 1 0.0 3 0 0 0 1 139 4 0.0 3 0 0 1 3 140 3 0.0 3 0 0 0 3 141 4 0.0 3 0 0 0 4 142 3 0.0 3 0 0 0 3 143 2 0.0 3 0 0 0 2 144 1 0.0 3 0 0 0 1 145 40 0.0 3 0 0 1 39 146 2 0.0 3 0 1 0 1 149 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 155 2 0.0 3 0 0 0 2 156 3 0.0 3 0 0 0 3 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 165 27 0.0 3 0 0 1 26 166 1 0.0 3 0 0 0 1 167 2 0.0 3 0 0 1 1 168 3 0.0 3 0 0 0 3 169 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 172 3 0.0 3 0 0 0 3 175 1 0.0 3 0 0 0 1 180 2 0.0 3 0 0 0 2 181 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 184 2 0.0 3 0 0 0 2 187 2 0.0 3 0 0 0 2 189 2 0.0 3 0 0 0 2 192 2 0.0 3 0 0 0 2 195 1 0.0 3 0 0 0 1 198 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 1 206 1 0.0 3 0 0 0 1 219 2 0.0 3 0 0 0 2 221 14 0.0 3 0 0 0 14 222 3 0.0 3 0 0 0 3 223 1 0.0 3 0 0 0 1 224 1 0.0 3 0 0 0 1 226 1 0.0 3 0 0 0 1 229 9 0.0 3 0 0 0 9 230 5 0.0 3 0 0 0 5 231 15 0.0 3 0 0 0 15 232 20 0.0 3 0 0 1 19 233 5 0.0 3 0 0 0 5 236 2 0.0 3 0 0 0 2 237 4 0.0 3 0 0 0 4 238 3 0.0 3 0 0 0 3 239 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 243 3 0.0 3 0 0 0 3 253 2 0.0 3 0 0 0 2 267 1 0.0 3 0 0 0 1 269 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 281 2 0.0 3 0 0 0 2 282 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.23 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 6,715 Reads with adapters: 19 (0.3%) Reads written (passing filters): 6,715 (100.0%) Total basepairs processed: 1,815,662 bp Total written (filtered): 1,815,605 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 19 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 42.1% C: 26.3% G: 10.5% T: 21.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 19 104.9 0 19