This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCCTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 68.02 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 229,848 (9.0%) Reads that were too short: 16,762 (0.7%) Reads written (passing filters): 213,086 (8.4%) Total basepairs processed: 766,381,518 bp Total written (filtered): 49,081,274 bp (6.4%) === Adapter 1 === Sequence: AGCCTC; Type: regular 5'; Length: 6; Trimmed: 229848 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 402 621.6 0 402 7 5829 621.6 0 5829 8 6892 621.6 0 6892 9 141 621.6 0 141 10 154 621.6 0 154 11 133 621.6 0 133 12 203 621.6 0 203 13 136 621.6 0 136 14 108 621.6 0 108 15 162 621.6 0 162 16 127 621.6 0 127 17 160 621.6 0 160 18 166 621.6 0 166 19 203 621.6 0 203 20 165 621.6 0 165 21 179 621.6 0 179 22 157 621.6 0 157 23 155 621.6 0 155 24 173 621.6 0 173 25 158 621.6 0 158 26 177 621.6 0 177 27 121 621.6 0 121 28 137 621.6 0 137 29 408 621.6 0 408 30 143 621.6 0 143 31 108 621.6 0 108 32 121 621.6 0 121 33 130 621.6 0 130 34 127 621.6 0 127 35 130 621.6 0 130 36 185 621.6 0 185 37 157 621.6 0 157 38 246 621.6 0 246 39 455 621.6 0 455 40 214 621.6 0 214 41 222 621.6 0 222 42 192 621.6 0 192 43 241 621.6 0 241 44 414 621.6 0 414 45 226 621.6 0 226 46 220 621.6 0 220 47 191 621.6 0 191 48 174 621.6 0 174 49 283 621.6 0 283 50 357 621.6 0 357 51 599 621.6 0 599 52 796 621.6 0 796 53 954 621.6 0 954 54 2633 621.6 0 2633 55 5916 621.6 0 5916 56 80439 621.6 0 80439 57 22957 621.6 0 22957 58 37343 621.6 0 37343 59 269 621.6 0 269 60 165 621.6 0 165 61 125 621.6 0 125 62 163 621.6 0 163 63 149 621.6 0 149 64 150 621.6 0 150 65 112 621.6 0 112 66 130 621.6 0 130 67 132 621.6 0 132 68 141 621.6 0 141 69 133 621.6 0 133 70 149 621.6 0 149 71 137 621.6 0 137 72 134 621.6 0 134 73 130 621.6 0 130 74 138 621.6 0 138 75 143 621.6 0 143 76 105 621.6 0 105 77 114 621.6 0 114 78 108 621.6 0 108 79 100 621.6 0 100 80 116 621.6 0 116 81 138 621.6 0 138 82 103 621.6 0 103 83 170 621.6 0 170 84 139 621.6 0 139 85 112 621.6 0 112 86 109 621.6 0 109 87 104 621.6 0 104 88 99 621.6 0 99 89 96 621.6 0 96 90 130 621.6 0 130 91 76 621.6 0 76 92 99 621.6 0 99 93 92 621.6 0 92 94 101 621.6 0 101 95 70 621.6 0 70 96 126 621.6 0 126 97 95 621.6 0 95 98 99 621.6 0 99 99 120 621.6 0 120 100 124 621.6 0 124 101 108 621.6 0 108 102 97 621.6 0 97 103 183 621.6 0 183 104 175 621.6 0 175 105 182 621.6 0 182 106 97 621.6 0 97 107 103 621.6 0 103 108 119 621.6 0 119 109 133 621.6 0 133 110 108 621.6 0 108 111 116 621.6 0 116 112 86 621.6 0 86 113 105 621.6 0 105 114 128 621.6 0 128 115 105 621.6 0 105 116 111 621.6 0 111 117 128 621.6 0 128 118 109 621.6 0 109 119 139 621.6 0 139 120 148 621.6 0 148 121 111 621.6 0 111 122 97 621.6 0 97 123 123 621.6 0 123 124 465 621.6 0 465 125 1452 621.6 0 1452 126 903 621.6 0 903 127 150 621.6 0 150 128 106 621.6 0 106 129 124 621.6 0 124 130 155 621.6 0 155 131 142 621.6 0 142 132 137 621.6 0 137 133 126 621.6 0 126 134 109 621.6 0 109 135 149 621.6 0 149 136 127 621.6 0 127 137 117 621.6 0 117 138 138 621.6 0 138 139 138 621.6 0 138 140 122 621.6 0 122 141 151 621.6 0 151 142 124 621.6 0 124 143 144 621.6 0 144 144 166 621.6 0 166 145 171 621.6 0 171 146 242 621.6 0 242 147 870 621.6 0 870 148 603 621.6 0 603 149 1626 621.6 0 1626 150 758 621.6 0 758 151 326 621.6 0 326 152 458 621.6 0 458 153 135 621.6 0 135 154 189 621.6 0 189 155 181 621.6 0 181 156 132 621.6 0 132 157 156 621.6 0 156 158 109 621.6 0 109 159 137 621.6 0 137 160 206 621.6 0 206 161 197 621.6 0 197 162 420 621.6 0 420 163 165 621.6 0 165 164 134 621.6 0 134 165 237 621.6 0 237 166 399 621.6 0 399 167 2676 621.6 0 2676 168 2633 621.6 0 2633 169 1416 621.6 0 1416 170 145 621.6 0 145 171 149 621.6 0 149 172 186 621.6 0 186 173 174 621.6 0 174 174 145 621.6 0 145 175 309 621.6 0 309 176 245 621.6 0 245 177 355 621.6 0 355 178 122 621.6 0 122 179 113 621.6 0 113 180 169 621.6 0 169 181 109 621.6 0 109 182 98 621.6 0 98 183 134 621.6 0 134 184 106 621.6 0 106 185 92 621.6 0 92 186 150 621.6 0 150 187 119 621.6 0 119 188 141 621.6 0 141 189 157 621.6 0 157 190 114 621.6 0 114 191 98 621.6 0 98 192 122 621.6 0 122 193 130 621.6 0 130 194 158 621.6 0 158 195 408 621.6 0 408 196 458 621.6 0 458 197 297 621.6 0 297 198 270 621.6 0 270 199 549 621.6 0 549 200 4622 621.6 0 4622 201 1946 621.6 0 1946 202 2612 621.6 0 2612 203 181 621.6 0 181 204 102 621.6 0 102 205 107 621.6 0 107 206 103 621.6 0 103 207 128 621.6 0 128 208 140 621.6 0 140 209 108 621.6 0 108 210 94 621.6 0 94 211 106 621.6 0 106 212 121 621.6 0 121 213 118 621.6 0 118 214 122 621.6 0 122 215 106 621.6 0 106 216 114 621.6 0 114 217 133 621.6 0 133 218 105 621.6 0 105 219 135 621.6 0 135 220 160 621.6 0 160 221 156 621.6 0 156 222 120 621.6 0 120 223 79 621.6 0 79 224 106 621.6 0 106 225 107 621.6 0 107 226 112 621.6 0 112 227 106 621.6 0 106 228 140 621.6 0 140 229 131 621.6 0 131 230 270 621.6 0 270 231 339 621.6 0 339 232 313 621.6 0 313 233 201 621.6 0 201 234 235 621.6 0 235 235 105 621.6 0 105 236 98 621.6 0 98 237 104 621.6 0 104 238 126 621.6 0 126 239 137 621.6 0 137 240 129 621.6 0 129 241 148 621.6 0 148 242 132 621.6 0 132 243 140 621.6 0 140 244 134 621.6 0 134 245 93 621.6 0 93 246 129 621.6 0 129 247 103 621.6 0 103 248 134 621.6 0 134 249 122 621.6 0 122 250 133 621.6 0 133 251 96 621.6 0 96 252 151 621.6 0 151 253 115 621.6 0 115 254 103 621.6 0 103 255 117 621.6 0 117 256 110 621.6 0 110 257 85 621.6 0 85 258 122 621.6 0 122 259 89 621.6 0 89 260 115 621.6 0 115 261 116 621.6 0 116 262 124 621.6 0 124 263 104 621.6 0 104 264 108 621.6 0 108 265 131 621.6 0 131 266 102 621.6 0 102 267 115 621.6 0 115 268 193 621.6 0 193 269 179 621.6 0 179 270 169 621.6 0 169 271 128 621.6 0 128 272 136 621.6 0 136 273 163 621.6 0 163 274 128 621.6 0 128 275 142 621.6 0 142 276 242 621.6 0 242 277 155 621.6 0 155 278 210 621.6 0 210 279 145 621.6 0 145 280 154 621.6 0 154 281 237 621.6 0 237 282 253 621.6 0 253 283 412 621.6 0 412 284 106 621.6 0 106 285 100 621.6 0 100 286 114 621.6 0 114 287 124 621.6 0 124 288 122 621.6 0 122 289 181 621.6 0 181 290 161 621.6 0 161 291 135 621.6 0 135 292 110 621.6 0 110 293 157 621.6 0 157 294 143 621.6 0 143 295 164 621.6 0 164 296 166 621.6 0 166 297 120 621.6 0 120 298 107 621.6 0 107 299 140 621.6 0 140 300 235 621.6 0 235 301 251 621.6 0 251 Finished in 9.47 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 213,086 Reads with adapters: 7,103 (3.3%) Reads that were too short: 34 (0.0%) Reads written (passing filters): 7,069 (3.3%) Total basepairs processed: 49,081,274 bp Total written (filtered): 1,926,236 bp (3.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 7103 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 2 0.0 3 0 0 2 19 99 0.0 3 0 97 2 20 6764 0.0 4 6679 81 4 21 20 0.0 4 2 18 22 5 0.0 4 0 3 2 23 146 0.0 4 134 5 0 7 24 1 0.0 4 0 0 0 0 1 26 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 1 94 1 0.0 4 0 0 0 0 1 102 1 0.0 4 1 106 1 0.0 4 1 117 1 0.0 4 0 0 0 0 1 122 2 0.0 4 0 0 0 0 2 123 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 127 25 0.0 4 0 0 0 0 25 144 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 0 1 147 1 0.0 4 1 176 1 0.0 4 0 1 202 24 0.0 4 0 0 0 1 23 221 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.25 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 7,069 Reads with adapters: 908 (12.8%) Reads written (passing filters): 7,069 (100.0%) Total basepairs processed: 1,926,236 bp Total written (filtered): 1,910,480 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 908 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.7% C: 5.6% G: 92.4% T: 1.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 110.5 0 1 4 1 27.6 0 1 9 25 0.0 0 2 23 10 114 0.0 1 3 111 11 713 0.0 1 1 712 12 1 0.0 1 0 1 19 1 0.0 1 0 1 90 31 0.0 1 30 1 170 1 0.0 1 1 177 16 0.0 1 16 178 1 0.0 1 0 1 180 3 0.0 1 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.