This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.74 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 301,432 (11.8%) Reads that were too short: 18,381 (0.7%) Reads written (passing filters): 283,051 (11.1%) Total basepairs processed: 766,381,518 bp Total written (filtered): 67,569,940 bp (8.8%) === Adapter 1 === Sequence: GTCAAT; Type: regular 5'; Length: 6; Trimmed: 301432 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 774 621.6 0 774 7 9863 621.6 0 9863 8 255 621.6 0 255 9 11197 621.6 0 11197 10 82 621.6 0 82 11 74 621.6 0 74 12 85 621.6 0 85 13 95 621.6 0 95 14 85 621.6 0 85 15 85 621.6 0 85 16 90 621.6 0 90 17 106 621.6 0 106 18 109 621.6 0 109 19 83 621.6 0 83 20 100 621.6 0 100 21 111 621.6 0 111 22 98 621.6 0 98 23 112 621.6 0 112 24 110 621.6 0 110 25 116 621.6 0 116 26 142 621.6 0 142 27 121 621.6 0 121 28 186 621.6 0 186 29 212 621.6 0 212 30 202 621.6 0 202 31 181 621.6 0 181 32 259 621.6 0 259 33 167 621.6 0 167 34 206 621.6 0 206 35 319 621.6 0 319 36 168 621.6 0 168 37 217 621.6 0 217 38 805 621.6 0 805 39 259 621.6 0 259 40 285 621.6 0 285 41 378 621.6 0 378 42 4706 621.6 0 4706 43 2801 621.6 0 2801 44 7164 621.6 0 7164 45 217 621.6 0 217 46 173 621.6 0 173 47 374 621.6 0 374 48 1116 621.6 0 1116 49 184 621.6 0 184 50 401 621.6 0 401 51 231 621.6 0 231 52 304 621.6 0 304 53 968 621.6 0 968 54 580 621.6 0 580 55 532 621.6 0 532 56 165 621.6 0 165 57 216 621.6 0 216 58 464 621.6 0 464 59 505 621.6 0 505 60 872 621.6 0 872 61 1394 621.6 0 1394 62 1274 621.6 0 1274 63 2961 621.6 0 2961 64 8400 621.6 0 8400 65 61378 621.6 0 61378 66 74301 621.6 0 74301 67 70236 621.6 0 70236 68 412 621.6 0 412 69 110 621.6 0 110 70 91 621.6 0 91 71 97 621.6 0 97 72 70 621.6 0 70 73 95 621.6 0 95 74 59 621.6 0 59 75 70 621.6 0 70 76 87 621.6 0 87 77 139 621.6 0 139 78 71 621.6 0 71 79 81 621.6 0 81 80 82 621.6 0 82 81 88 621.6 0 88 82 77 621.6 0 77 83 105 621.6 0 105 84 72 621.6 0 72 85 74 621.6 0 74 86 68 621.6 0 68 87 81 621.6 0 81 88 84 621.6 0 84 89 97 621.6 0 97 90 88 621.6 0 88 91 78 621.6 0 78 92 86 621.6 0 86 93 99 621.6 0 99 94 95 621.6 0 95 95 98 621.6 0 98 96 98 621.6 0 98 97 78 621.6 0 78 98 139 621.6 0 139 99 103 621.6 0 103 100 148 621.6 0 148 101 133 621.6 0 133 102 145 621.6 0 145 103 75 621.6 0 75 104 79 621.6 0 79 105 88 621.6 0 88 106 69 621.6 0 69 107 69 621.6 0 69 108 94 621.6 0 94 109 74 621.6 0 74 110 159 621.6 0 159 111 84 621.6 0 84 112 103 621.6 0 103 113 86 621.6 0 86 114 80 621.6 0 80 115 79 621.6 0 79 116 65 621.6 0 65 117 89 621.6 0 89 118 74 621.6 0 74 119 80 621.6 0 80 120 78 621.6 0 78 121 103 621.6 0 103 122 66 621.6 0 66 123 82 621.6 0 82 124 107 621.6 0 107 125 82 621.6 0 82 126 84 621.6 0 84 127 98 621.6 0 98 128 73 621.6 0 73 129 92 621.6 0 92 130 111 621.6 0 111 131 87 621.6 0 87 132 79 621.6 0 79 133 100 621.6 0 100 134 698 621.6 0 698 135 631 621.6 0 631 136 328 621.6 0 328 137 125 621.6 0 125 138 116 621.6 0 116 139 116 621.6 0 116 140 86 621.6 0 86 141 75 621.6 0 75 142 80 621.6 0 80 143 71 621.6 0 71 144 68 621.6 0 68 145 53 621.6 0 53 146 77 621.6 0 77 147 87 621.6 0 87 148 84 621.6 0 84 149 67 621.6 0 67 150 44 621.6 0 44 151 58 621.6 0 58 152 57 621.6 0 57 153 78 621.6 0 78 154 74 621.6 0 74 155 66 621.6 0 66 156 55 621.6 0 55 157 76 621.6 0 76 158 102 621.6 0 102 159 90 621.6 0 90 160 93 621.6 0 93 161 71 621.6 0 71 162 119 621.6 0 119 163 73 621.6 0 73 164 64 621.6 0 64 165 71 621.6 0 71 166 64 621.6 0 64 167 73 621.6 0 73 168 77 621.6 0 77 169 83 621.6 0 83 170 48 621.6 0 48 171 62 621.6 0 62 172 72 621.6 0 72 173 72 621.6 0 72 174 123 621.6 0 123 175 75 621.6 0 75 176 66 621.6 0 66 177 59 621.6 0 59 178 113 621.6 0 113 179 112 621.6 0 112 180 121 621.6 0 121 181 104 621.6 0 104 182 84 621.6 0 84 183 141 621.6 0 141 184 149 621.6 0 149 185 131 621.6 0 131 186 118 621.6 0 118 187 61 621.6 0 61 188 58 621.6 0 58 189 69 621.6 0 69 190 69 621.6 0 69 191 90 621.6 0 90 192 65 621.6 0 65 193 59 621.6 0 59 194 57 621.6 0 57 195 74 621.6 0 74 196 71 621.6 0 71 197 101 621.6 0 101 198 100 621.6 0 100 199 93 621.6 0 93 200 124 621.6 0 124 201 119 621.6 0 119 202 171 621.6 0 171 203 167 621.6 0 167 204 94 621.6 0 94 205 82 621.6 0 82 206 83 621.6 0 83 207 82 621.6 0 82 208 77 621.6 0 77 209 93 621.6 0 93 210 60 621.6 0 60 211 77 621.6 0 77 212 76 621.6 0 76 213 85 621.6 0 85 214 84 621.6 0 84 215 70 621.6 0 70 216 104 621.6 0 104 217 95 621.6 0 95 218 99 621.6 0 99 219 125 621.6 0 125 220 133 621.6 0 133 221 158 621.6 0 158 222 995 621.6 0 995 223 986 621.6 0 986 224 808 621.6 0 808 225 272 621.6 0 272 226 75 621.6 0 75 227 75 621.6 0 75 228 91 621.6 0 91 229 64 621.6 0 64 230 58 621.6 0 58 231 68 621.6 0 68 232 59 621.6 0 59 233 65 621.6 0 65 234 74 621.6 0 74 235 73 621.6 0 73 236 92 621.6 0 92 237 82 621.6 0 82 238 93 621.6 0 93 239 72 621.6 0 72 240 104 621.6 0 104 241 100 621.6 0 100 242 311 621.6 0 311 243 311 621.6 0 311 244 255 621.6 0 255 245 138 621.6 0 138 246 93 621.6 0 93 247 75 621.6 0 75 248 75 621.6 0 75 249 80 621.6 0 80 250 85 621.6 0 85 251 141 621.6 0 141 252 159 621.6 0 159 253 251 621.6 0 251 254 327 621.6 0 327 255 1120 621.6 0 1120 256 595 621.6 0 595 257 1887 621.6 0 1887 258 167 621.6 0 167 259 117 621.6 0 117 260 324 621.6 0 324 261 485 621.6 0 485 262 644 621.6 0 644 263 131 621.6 0 131 264 102 621.6 0 102 265 161 621.6 0 161 266 202 621.6 0 202 267 81 621.6 0 81 268 73 621.6 0 73 269 175 621.6 0 175 270 426 621.6 0 426 271 227 621.6 0 227 272 286 621.6 0 286 273 75 621.6 0 75 274 82 621.6 0 82 275 83 621.6 0 83 276 129 621.6 0 129 277 197 621.6 0 197 278 197 621.6 0 197 279 153 621.6 0 153 280 64 621.6 0 64 281 68 621.6 0 68 282 57 621.6 0 57 283 60 621.6 0 60 284 65 621.6 0 65 285 73 621.6 0 73 286 55 621.6 0 55 287 60 621.6 0 60 288 66 621.6 0 66 289 46 621.6 0 46 290 70 621.6 0 70 291 89 621.6 0 89 292 109 621.6 0 109 293 80 621.6 0 80 294 69 621.6 0 69 295 56 621.6 0 56 296 53 621.6 0 53 297 64 621.6 0 64 298 88 621.6 0 88 299 115 621.6 0 115 300 127 621.6 0 127 301 106 621.6 0 106 Finished in 11.16 s (39 us/read; 1.52 M reads/minute). === Summary === Total reads processed: 283,051 Reads with adapters: 11,890 (4.2%) Reads that were too short: 136 (0.0%) Reads written (passing filters): 11,754 (4.2%) Total basepairs processed: 67,569,940 bp Total written (filtered): 3,214,579 bp (4.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 11890 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 3 0.0 2 0 0 0 3 15 9 0.0 3 0 0 5 4 16 156 0.0 3 0 141 11 4 17 11167 0.0 3 10396 631 116 24 18 35 0.0 3 3 17 8 7 19 30 0.0 3 1 4 11 14 20 232 0.0 3 179 16 2 35 22 1 0.0 3 0 0 1 29 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 0 2 34 3 0.0 3 0 0 0 3 35 1 0.0 3 0 0 1 37 1 0.0 3 0 0 0 1 38 2 0.0 3 0 0 1 1 41 5 0.0 3 0 0 1 4 54 1 0.0 3 0 0 0 1 55 4 0.0 3 0 0 1 3 57 8 0.0 3 0 0 0 8 58 3 0.0 3 0 0 0 3 59 2 0.0 3 0 0 0 2 60 4 0.0 3 0 0 0 4 61 5 0.0 3 0 0 0 5 66 1 0.0 3 0 0 0 1 69 1 0.0 3 1 72 1 0.0 3 0 0 1 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 79 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 2 0.0 3 0 0 0 2 82 5 0.0 3 0 0 0 5 85 3 0.0 3 0 0 0 3 87 1 0.0 3 1 88 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 92 2 0.0 3 0 0 0 2 93 1 0.0 3 0 0 1 97 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 102 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 107 4 0.0 3 0 0 0 4 108 6 0.0 3 0 0 0 6 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 113 2 0.0 3 0 0 0 2 120 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 3 0.0 3 0 0 0 3 127 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 129 1 0.0 3 0 0 0 1 130 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 134 2 0.0 3 0 0 0 2 136 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 0 2 144 15 0.0 3 0 0 0 15 145 1 0.0 3 0 0 0 1 146 2 0.0 3 0 0 0 2 147 4 0.0 3 0 0 0 4 148 5 0.0 3 0 0 0 5 149 9 0.0 3 0 0 0 9 150 1 0.0 3 0 0 0 1 152 2 0.0 3 0 0 1 1 155 1 0.0 3 0 0 0 1 158 2 0.0 3 0 0 0 2 159 5 0.0 3 0 0 1 4 160 2 0.0 3 0 0 0 2 165 1 0.0 3 0 0 0 1 166 2 0.0 3 0 0 1 1 167 7 0.0 3 0 0 1 6 168 7 0.0 3 0 0 0 7 170 1 0.0 3 0 0 0 1 173 2 0.0 3 0 0 0 2 176 1 0.0 3 0 0 0 1 177 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 204 1 0.0 3 0 1 205 8 0.0 3 0 0 0 8 210 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 216 1 0.0 3 0 0 0 1 218 2 0.0 3 0 0 0 2 221 6 0.0 3 0 0 0 6 222 7 0.0 3 0 0 0 7 223 8 0.0 3 0 0 0 8 224 13 0.0 3 0 0 2 11 225 9 0.0 3 0 0 1 8 227 1 0.0 3 0 0 0 1 230 2 0.0 3 0 0 0 2 231 3 0.0 3 0 0 0 3 233 1 0.0 3 0 0 0 1 234 4 0.0 3 0 0 0 4 237 1 0.0 3 0 0 0 1 246 1 0.0 3 0 0 0 1 250 1 0.0 3 0 0 0 1 251 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 257 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 262 1 0.0 3 0 0 0 1 279 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.41 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 11,754 Reads with adapters: 273 (2.3%) Reads written (passing filters): 11,754 (100.0%) Total basepairs processed: 3,214,579 bp Total written (filtered): 3,213,739 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 273 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 60.4% C: 17.6% G: 19.0% T: 2.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 261 183.7 0 261 4 11 45.9 0 11 13 1 0.0 1 0 1