This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTATGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.89 s (26 us/read; 2.32 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 142,675 (5.6%) Reads that were too short: 24,246 (1.0%) Reads written (passing filters): 118,429 (4.7%) Total basepairs processed: 766,381,518 bp Total written (filtered): 31,598,290 bp (4.1%) === Adapter 1 === Sequence: GTATGT; Type: regular 5'; Length: 6; Trimmed: 142675 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2776 621.6 0 2776 7 1087 621.6 0 1087 8 46997 621.6 0 46997 9 38510 621.6 0 38510 10 112 621.6 0 112 11 84 621.6 0 84 12 77 621.6 0 77 13 89 621.6 0 89 14 67 621.6 0 67 15 76 621.6 0 76 16 106 621.6 0 106 17 108 621.6 0 108 18 89 621.6 0 89 19 93 621.6 0 93 20 88 621.6 0 88 21 64 621.6 0 64 22 119 621.6 0 119 23 108 621.6 0 108 24 225 621.6 0 225 25 151 621.6 0 151 26 132 621.6 0 132 27 86 621.6 0 86 28 99 621.6 0 99 29 139 621.6 0 139 30 108 621.6 0 108 31 95 621.6 0 95 32 62 621.6 0 62 33 70 621.6 0 70 34 109 621.6 0 109 35 116 621.6 0 116 36 156 621.6 0 156 37 87 621.6 0 87 38 91 621.6 0 91 39 80 621.6 0 80 40 83 621.6 0 83 41 81 621.6 0 81 42 95 621.6 0 95 43 103 621.6 0 103 44 80 621.6 0 80 45 86 621.6 0 86 46 87 621.6 0 87 47 78 621.6 0 78 48 78 621.6 0 78 49 99 621.6 0 99 50 91 621.6 0 91 51 78 621.6 0 78 52 97 621.6 0 97 53 107 621.6 0 107 54 104 621.6 0 104 55 75 621.6 0 75 56 83 621.6 0 83 57 99 621.6 0 99 58 116 621.6 0 116 59 61 621.6 0 61 60 66 621.6 0 66 61 69 621.6 0 69 62 75 621.6 0 75 63 128 621.6 0 128 64 101 621.6 0 101 65 120 621.6 0 120 66 105 621.6 0 105 67 159 621.6 0 159 68 134 621.6 0 134 69 298 621.6 0 298 70 111 621.6 0 111 71 174 621.6 0 174 72 108 621.6 0 108 73 98 621.6 0 98 74 99 621.6 0 99 75 101 621.6 0 101 76 202 621.6 0 202 77 202 621.6 0 202 78 249 621.6 0 249 79 198 621.6 0 198 80 581 621.6 0 581 81 518 621.6 0 518 82 470 621.6 0 470 83 116 621.6 0 116 84 97 621.6 0 97 85 117 621.6 0 117 86 130 621.6 0 130 87 105 621.6 0 105 88 80 621.6 0 80 89 109 621.6 0 109 90 104 621.6 0 104 91 105 621.6 0 105 92 96 621.6 0 96 93 133 621.6 0 133 94 113 621.6 0 113 95 156 621.6 0 156 96 1344 621.6 0 1344 97 389 621.6 0 389 98 1081 621.6 0 1081 99 147 621.6 0 147 100 126 621.6 0 126 101 106 621.6 0 106 102 114 621.6 0 114 103 86 621.6 0 86 104 91 621.6 0 91 105 107 621.6 0 107 106 91 621.6 0 91 107 100 621.6 0 100 108 180 621.6 0 180 109 110 621.6 0 110 110 121 621.6 0 121 111 109 621.6 0 109 112 110 621.6 0 110 113 125 621.6 0 125 114 135 621.6 0 135 115 112 621.6 0 112 116 90 621.6 0 90 117 100 621.6 0 100 118 81 621.6 0 81 119 88 621.6 0 88 120 105 621.6 0 105 121 80 621.6 0 80 122 102 621.6 0 102 123 93 621.6 0 93 124 103 621.6 0 103 125 109 621.6 0 109 126 110 621.6 0 110 127 168 621.6 0 168 128 158 621.6 0 158 129 180 621.6 0 180 130 241 621.6 0 241 131 188 621.6 0 188 132 403 621.6 0 403 133 204 621.6 0 204 134 336 621.6 0 336 135 128 621.6 0 128 136 109 621.6 0 109 137 139 621.6 0 139 138 123 621.6 0 123 139 113 621.6 0 113 140 126 621.6 0 126 141 103 621.6 0 103 142 91 621.6 0 91 143 124 621.6 0 124 144 110 621.6 0 110 145 87 621.6 0 87 146 103 621.6 0 103 147 123 621.6 0 123 148 257 621.6 0 257 149 156 621.6 0 156 150 821 621.6 0 821 151 93 621.6 0 93 152 82 621.6 0 82 153 104 621.6 0 104 154 80 621.6 0 80 155 74 621.6 0 74 156 107 621.6 0 107 157 84 621.6 0 84 158 108 621.6 0 108 159 95 621.6 0 95 160 120 621.6 0 120 161 136 621.6 0 136 162 119 621.6 0 119 163 87 621.6 0 87 164 98 621.6 0 98 165 92 621.6 0 92 166 98 621.6 0 98 167 107 621.6 0 107 168 103 621.6 0 103 169 97 621.6 0 97 170 101 621.6 0 101 171 83 621.6 0 83 172 101 621.6 0 101 173 96 621.6 0 96 174 86 621.6 0 86 175 85 621.6 0 85 176 116 621.6 0 116 177 87 621.6 0 87 178 90 621.6 0 90 179 87 621.6 0 87 180 97 621.6 0 97 181 133 621.6 0 133 182 95 621.6 0 95 183 134 621.6 0 134 184 130 621.6 0 130 185 182 621.6 0 182 186 210 621.6 0 210 187 1351 621.6 0 1351 188 604 621.6 0 604 189 482 621.6 0 482 190 611 621.6 0 611 191 94 621.6 0 94 192 100 621.6 0 100 193 80 621.6 0 80 194 88 621.6 0 88 195 103 621.6 0 103 196 127 621.6 0 127 197 103 621.6 0 103 198 109 621.6 0 109 199 94 621.6 0 94 200 86 621.6 0 86 201 100 621.6 0 100 202 92 621.6 0 92 203 120 621.6 0 120 204 96 621.6 0 96 205 124 621.6 0 124 206 97 621.6 0 97 207 144 621.6 0 144 208 125 621.6 0 125 209 466 621.6 0 466 210 734 621.6 0 734 211 519 621.6 0 519 212 246 621.6 0 246 213 120 621.6 0 120 214 119 621.6 0 119 215 79 621.6 0 79 216 86 621.6 0 86 217 97 621.6 0 97 218 78 621.6 0 78 219 127 621.6 0 127 220 106 621.6 0 106 221 106 621.6 0 106 222 96 621.6 0 96 223 214 621.6 0 214 224 172 621.6 0 172 225 163 621.6 0 163 226 251 621.6 0 251 227 295 621.6 0 295 228 209 621.6 0 209 229 110 621.6 0 110 230 138 621.6 0 138 231 127 621.6 0 127 232 146 621.6 0 146 233 169 621.6 0 169 234 171 621.6 0 171 235 227 621.6 0 227 236 190 621.6 0 190 237 144 621.6 0 144 238 142 621.6 0 142 239 154 621.6 0 154 240 612 621.6 0 612 241 466 621.6 0 466 242 719 621.6 0 719 243 313 621.6 0 313 244 408 621.6 0 408 245 199 621.6 0 199 246 483 621.6 0 483 247 288 621.6 0 288 248 793 621.6 0 793 249 761 621.6 0 761 250 407 621.6 0 407 251 540 621.6 0 540 252 210 621.6 0 210 253 679 621.6 0 679 254 855 621.6 0 855 255 648 621.6 0 648 256 250 621.6 0 250 257 165 621.6 0 165 258 241 621.6 0 241 259 159 621.6 0 159 260 479 621.6 0 479 261 651 621.6 0 651 262 365 621.6 0 365 263 159 621.6 0 159 264 89 621.6 0 89 265 343 621.6 0 343 266 236 621.6 0 236 267 246 621.6 0 246 268 206 621.6 0 206 269 528 621.6 0 528 270 658 621.6 0 658 271 388 621.6 0 388 272 391 621.6 0 391 273 409 621.6 0 409 274 329 621.6 0 329 275 109 621.6 0 109 276 80 621.6 0 80 277 74 621.6 0 74 278 80 621.6 0 80 279 103 621.6 0 103 280 109 621.6 0 109 281 130 621.6 0 130 282 107 621.6 0 107 283 158 621.6 0 158 284 155 621.6 0 155 285 140 621.6 0 140 286 83 621.6 0 83 287 71 621.6 0 71 288 62 621.6 0 62 289 67 621.6 0 67 290 100 621.6 0 100 291 77 621.6 0 77 292 99 621.6 0 99 293 107 621.6 0 107 294 144 621.6 0 144 295 100 621.6 0 100 296 112 621.6 0 112 297 75 621.6 0 75 298 101 621.6 0 101 299 90 621.6 0 90 300 111 621.6 0 111 301 130 621.6 0 130 Finished in 5.46 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 118,429 Reads with adapters: 48,969 (41.3%) Reads that were too short: 100 (0.1%) Reads written (passing filters): 48,869 (41.3%) Total basepairs processed: 31,598,290 bp Total written (filtered): 13,467,898 bp (42.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 48969 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 12 0.0 2 0 0 0 12 15 42 0.0 3 0 0 27 15 16 586 0.0 3 0 500 56 30 17 45556 0.0 3 42257 2660 508 131 18 141 0.0 3 7 87 20 27 19 192 0.0 3 19 16 87 70 20 2197 0.0 3 1865 146 34 152 21 6 0.0 3 1 5 22 3 0.0 3 0 0 2 1 23 3 0.0 3 0 0 0 3 24 1 0.0 3 0 0 1 34 3 0.0 3 0 0 0 3 37 2 0.0 3 0 0 0 2 38 2 0.0 3 0 0 0 2 42 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 59 7 0.0 3 0 0 0 7 60 1 0.0 3 0 0 0 1 61 2 0.0 3 0 0 1 1 62 3 0.0 3 0 0 1 2 64 1 0.0 3 0 0 0 1 65 3 0.0 3 0 0 0 3 66 1 0.0 3 0 0 0 1 70 58 0.0 3 0 0 1 57 72 3 0.0 3 0 0 0 3 73 4 0.0 3 0 0 0 4 75 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 1 81 1 0.0 3 0 0 0 1 82 2 0.0 3 0 0 0 2 83 4 0.0 3 0 0 0 4 84 3 0.0 3 0 0 0 3 85 3 0.0 3 0 0 0 3 88 1 0.0 3 0 0 0 1 89 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 96 3 0.0 3 0 0 1 2 110 1 0.0 3 0 0 0 1 113 6 0.0 3 0 0 0 6 114 13 0.0 3 0 0 0 13 115 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 1 121 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 130 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 140 2 0.0 3 0 0 0 2 148 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 160 2 0.0 3 0 0 0 2 163 3 0.0 3 0 0 0 3 164 3 0.0 3 0 0 0 3 166 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 171 3 0.0 3 0 0 1 2 173 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 183 3 0.0 3 0 0 0 3 185 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 194 4 0.0 3 0 0 0 4 202 1 0.0 3 0 0 0 1 203 1 0.0 3 0 0 0 1 205 3 0.0 3 0 0 0 3 209 1 0.0 3 0 0 0 1 213 1 0.0 3 0 0 0 1 215 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 224 1 0.0 3 0 0 0 1 231 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 1 238 1 0.0 3 0 0 0 1 253 1 0.0 3 0 0 0 1 254 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 279 4 0.0 3 0 0 0 4 280 8 0.0 3 0 0 1 7 281 4 0.0 3 0 0 1 3 282 11 0.0 3 0 0 0 11 284 2 0.0 3 0 0 0 2 288 1 0.0 3 0 0 0 1 290 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.71 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 48,869 Reads with adapters: 518 (1.1%) Reads written (passing filters): 48,869 (100.0%) Total basepairs processed: 13,467,898 bp Total written (filtered): 13,466,146 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 518 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 35.9% C: 14.1% G: 47.1% T: 2.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 324 763.6 0 324 4 191 190.9 0 191 5 2 47.7 0 2 6 1 11.9 0 1