This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTATGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.22 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 129,918 (5.1%) Reads that were too short: 15,642 (0.6%) Reads written (passing filters): 114,276 (4.5%) Total basepairs processed: 766,381,518 bp Total written (filtered): 31,094,374 bp (4.1%) === Adapter 1 === Sequence: GTATGT; Type: regular 5'; Length: 6; Trimmed: 129918 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2429 621.6 0 2429 7 887 621.6 0 887 8 42452 621.6 0 42452 9 46766 621.6 0 46766 10 97 621.6 0 97 11 68 621.6 0 68 12 70 621.6 0 70 13 59 621.6 0 59 14 45 621.6 0 45 15 49 621.6 0 49 16 66 621.6 0 66 17 53 621.6 0 53 18 64 621.6 0 64 19 68 621.6 0 68 20 78 621.6 0 78 21 47 621.6 0 47 22 57 621.6 0 57 23 52 621.6 0 52 24 71 621.6 0 71 25 53 621.6 0 53 26 63 621.6 0 63 27 69 621.6 0 69 28 77 621.6 0 77 29 90 621.6 0 90 30 63 621.6 0 63 31 49 621.6 0 49 32 31 621.6 0 31 33 50 621.6 0 50 34 73 621.6 0 73 35 64 621.6 0 64 36 79 621.6 0 79 37 64 621.6 0 64 38 83 621.6 0 83 39 76 621.6 0 76 40 68 621.6 0 68 41 51 621.6 0 51 42 75 621.6 0 75 43 73 621.6 0 73 44 71 621.6 0 71 45 56 621.6 0 56 46 79 621.6 0 79 47 67 621.6 0 67 48 66 621.6 0 66 49 75 621.6 0 75 50 53 621.6 0 53 51 90 621.6 0 90 52 60 621.6 0 60 53 72 621.6 0 72 54 66 621.6 0 66 55 59 621.6 0 59 56 49 621.6 0 49 57 63 621.6 0 63 58 79 621.6 0 79 59 50 621.6 0 50 60 53 621.6 0 53 61 57 621.6 0 57 62 57 621.6 0 57 63 76 621.6 0 76 64 79 621.6 0 79 65 87 621.6 0 87 66 68 621.6 0 68 67 94 621.6 0 94 68 106 621.6 0 106 69 303 621.6 0 303 70 75 621.6 0 75 71 122 621.6 0 122 72 73 621.6 0 73 73 65 621.6 0 65 74 43 621.6 0 43 75 70 621.6 0 70 76 129 621.6 0 129 77 140 621.6 0 140 78 139 621.6 0 139 79 114 621.6 0 114 80 526 621.6 0 526 81 502 621.6 0 502 82 467 621.6 0 467 83 73 621.6 0 73 84 68 621.6 0 68 85 96 621.6 0 96 86 83 621.6 0 83 87 66 621.6 0 66 88 59 621.6 0 59 89 63 621.6 0 63 90 77 621.6 0 77 91 78 621.6 0 78 92 71 621.6 0 71 93 73 621.6 0 73 94 92 621.6 0 92 95 123 621.6 0 123 96 1002 621.6 0 1002 97 321 621.6 0 321 98 837 621.6 0 837 99 100 621.6 0 100 100 87 621.6 0 87 101 81 621.6 0 81 102 91 621.6 0 91 103 88 621.6 0 88 104 54 621.6 0 54 105 58 621.6 0 58 106 69 621.6 0 69 107 75 621.6 0 75 108 200 621.6 0 200 109 102 621.6 0 102 110 85 621.6 0 85 111 69 621.6 0 69 112 72 621.6 0 72 113 81 621.6 0 81 114 74 621.6 0 74 115 91 621.6 0 91 116 75 621.6 0 75 117 88 621.6 0 88 118 72 621.6 0 72 119 71 621.6 0 71 120 80 621.6 0 80 121 86 621.6 0 86 122 54 621.6 0 54 123 82 621.6 0 82 124 77 621.6 0 77 125 66 621.6 0 66 126 67 621.6 0 67 127 106 621.6 0 106 128 116 621.6 0 116 129 82 621.6 0 82 130 157 621.6 0 157 131 160 621.6 0 160 132 315 621.6 0 315 133 107 621.6 0 107 134 216 621.6 0 216 135 95 621.6 0 95 136 78 621.6 0 78 137 115 621.6 0 115 138 83 621.6 0 83 139 97 621.6 0 97 140 80 621.6 0 80 141 65 621.6 0 65 142 63 621.6 0 63 143 97 621.6 0 97 144 70 621.6 0 70 145 67 621.6 0 67 146 73 621.6 0 73 147 88 621.6 0 88 148 237 621.6 0 237 149 147 621.6 0 147 150 739 621.6 0 739 151 60 621.6 0 60 152 62 621.6 0 62 153 68 621.6 0 68 154 67 621.6 0 67 155 66 621.6 0 66 156 81 621.6 0 81 157 63 621.6 0 63 158 57 621.6 0 57 159 48 621.6 0 48 160 58 621.6 0 58 161 80 621.6 0 80 162 70 621.6 0 70 163 58 621.6 0 58 164 70 621.6 0 70 165 71 621.6 0 71 166 64 621.6 0 64 167 79 621.6 0 79 168 48 621.6 0 48 169 70 621.6 0 70 170 92 621.6 0 92 171 69 621.6 0 69 172 74 621.6 0 74 173 75 621.6 0 75 174 66 621.6 0 66 175 79 621.6 0 79 176 82 621.6 0 82 177 56 621.6 0 56 178 70 621.6 0 70 179 69 621.6 0 69 180 62 621.6 0 62 181 85 621.6 0 85 182 50 621.6 0 50 183 80 621.6 0 80 184 95 621.6 0 95 185 111 621.6 0 111 186 141 621.6 0 141 187 1110 621.6 0 1110 188 605 621.6 0 605 189 416 621.6 0 416 190 633 621.6 0 633 191 75 621.6 0 75 192 64 621.6 0 64 193 51 621.6 0 51 194 65 621.6 0 65 195 65 621.6 0 65 196 93 621.6 0 93 197 64 621.6 0 64 198 70 621.6 0 70 199 75 621.6 0 75 200 61 621.6 0 61 201 74 621.6 0 74 202 54 621.6 0 54 203 83 621.6 0 83 204 56 621.6 0 56 205 93 621.6 0 93 206 77 621.6 0 77 207 84 621.6 0 84 208 107 621.6 0 107 209 236 621.6 0 236 210 926 621.6 0 926 211 225 621.6 0 225 212 125 621.6 0 125 213 74 621.6 0 74 214 88 621.6 0 88 215 66 621.6 0 66 216 58 621.6 0 58 217 68 621.6 0 68 218 59 621.6 0 59 219 95 621.6 0 95 220 62 621.6 0 62 221 65 621.6 0 65 222 76 621.6 0 76 223 207 621.6 0 207 224 131 621.6 0 131 225 107 621.6 0 107 226 195 621.6 0 195 227 170 621.6 0 170 228 151 621.6 0 151 229 60 621.6 0 60 230 82 621.6 0 82 231 94 621.6 0 94 232 78 621.6 0 78 233 93 621.6 0 93 234 104 621.6 0 104 235 126 621.6 0 126 236 119 621.6 0 119 237 82 621.6 0 82 238 77 621.6 0 77 239 74 621.6 0 74 240 231 621.6 0 231 241 223 621.6 0 223 242 350 621.6 0 350 243 192 621.6 0 192 244 223 621.6 0 223 245 140 621.6 0 140 246 434 621.6 0 434 247 241 621.6 0 241 248 781 621.6 0 781 249 469 621.6 0 469 250 253 621.6 0 253 251 324 621.6 0 324 252 151 621.6 0 151 253 356 621.6 0 356 254 533 621.6 0 533 255 381 621.6 0 381 256 143 621.6 0 143 257 90 621.6 0 90 258 103 621.6 0 103 259 53 621.6 0 53 260 237 621.6 0 237 261 453 621.6 0 453 262 232 621.6 0 232 263 77 621.6 0 77 264 45 621.6 0 45 265 169 621.6 0 169 266 113 621.6 0 113 267 91 621.6 0 91 268 155 621.6 0 155 269 357 621.6 0 357 270 576 621.6 0 576 271 253 621.6 0 253 272 223 621.6 0 223 273 209 621.6 0 209 274 126 621.6 0 126 275 98 621.6 0 98 276 58 621.6 0 58 277 50 621.6 0 50 278 59 621.6 0 59 279 51 621.6 0 51 280 53 621.6 0 53 281 72 621.6 0 72 282 77 621.6 0 77 283 77 621.6 0 77 284 69 621.6 0 69 285 76 621.6 0 76 286 58 621.6 0 58 287 64 621.6 0 64 288 70 621.6 0 70 289 63 621.6 0 63 290 58 621.6 0 58 291 56 621.6 0 56 292 88 621.6 0 88 293 74 621.6 0 74 294 93 621.6 0 93 295 71 621.6 0 71 296 72 621.6 0 72 297 70 621.6 0 70 298 86 621.6 0 86 299 70 621.6 0 70 300 68 621.6 0 68 301 127 621.6 0 127 Finished in 5.79 s (51 us/read; 1.18 M reads/minute). === Summary === Total reads processed: 114,276 Reads with adapters: 48,644 (42.6%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 48,625 (42.6%) Total basepairs processed: 31,094,374 bp Total written (filtered): 13,210,300 bp (42.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 48644 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 24 0.0 3 0 0 22 1 1 19 648 0.0 3 0 635 12 0 1 20 47622 0.0 4 46842 762 17 1 21 96 0.0 4 9 85 2 22 14 0.0 4 2 0 10 0 2 23 45 0.0 4 0 0 0 43 2 28 1 0.0 4 0 0 0 0 1 33 1 0.0 4 1 41 2 0.0 4 0 0 0 0 2 56 3 0.0 4 0 0 0 0 3 58 4 0.0 4 0 0 0 0 4 88 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 154 0.0 4 0 0 0 0 154 128 1 0.0 4 0 0 0 0 1 130 12 0.0 4 0 0 0 0 12 150 1 0.0 4 1 153 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 1 216 1 0.0 4 0 0 0 0 1 228 1 0.0 4 0 0 0 0 1 232 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 261 1 0.0 4 0 0 0 0 1 275 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.74 s (36 us/read; 1.68 M reads/minute). === Summary === Total reads processed: 48,625 Reads with adapters: 6,237 (12.8%) Reads written (passing filters): 48,625 (100.0%) Total basepairs processed: 13,210,300 bp Total written (filtered): 13,148,020 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6237 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.9% C: 2.7% G: 94.9% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 8 759.8 0 8 4 7 189.9 0 7 5 1 47.5 0 1 7 3 3.0 0 3 9 2593 0.2 0 77 2516 10 2870 0.0 1 113 2757 11 725 0.0 1 5 720 12 7 0.0 1 0 7 13 3 0.0 1 0 3 19 9 0.0 1 8 1 104 1 0.0 1 1 138 1 0.0 1 1 150 2 0.0 1 0 2 162 1 0.0 1 1 169 1 0.0 1 1 191 1 0.0 1 0 1 208 4 0.0 1 2 2 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.