This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTTGCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.61 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 40,816 (1.6%) Reads that were too short: 13,442 (0.5%) Reads written (passing filters): 27,374 (1.1%) Total basepairs processed: 766,381,518 bp Total written (filtered): 5,963,600 bp (0.8%) === Adapter 1 === Sequence: GTTGCC; Type: regular 5'; Length: 6; Trimmed: 40816 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 194 621.6 0 194 7 4663 621.6 0 4663 8 3788 621.6 0 3788 9 78 621.6 0 78 10 342 621.6 0 342 11 57 621.6 0 57 12 59 621.6 0 59 13 45 621.6 0 45 14 70 621.6 0 70 15 39 621.6 0 39 16 55 621.6 0 55 17 35 621.6 0 35 18 35 621.6 0 35 19 45 621.6 0 45 20 59 621.6 0 59 21 53 621.6 0 53 22 64 621.6 0 64 23 53 621.6 0 53 24 56 621.6 0 56 25 57 621.6 0 57 26 44 621.6 0 44 27 39 621.6 0 39 28 31 621.6 0 31 29 50 621.6 0 50 30 67 621.6 0 67 31 55 621.6 0 55 32 37 621.6 0 37 33 39 621.6 0 39 34 51 621.6 0 51 35 40 621.6 0 40 36 64 621.6 0 64 37 72 621.6 0 72 38 132 621.6 0 132 39 81 621.6 0 81 40 100 621.6 0 100 41 67 621.6 0 67 42 48 621.6 0 48 43 44 621.6 0 44 44 44 621.6 0 44 45 39 621.6 0 39 46 56 621.6 0 56 47 47 621.6 0 47 48 162 621.6 0 162 49 77 621.6 0 77 50 38 621.6 0 38 51 47 621.6 0 47 52 50 621.6 0 50 53 40 621.6 0 40 54 51 621.6 0 51 55 62 621.6 0 62 56 55 621.6 0 55 57 51 621.6 0 51 58 46 621.6 0 46 59 49 621.6 0 49 60 95 621.6 0 95 61 82 621.6 0 82 62 68 621.6 0 68 63 38 621.6 0 38 64 49 621.6 0 49 65 64 621.6 0 64 66 49 621.6 0 49 67 56 621.6 0 56 68 81 621.6 0 81 69 214 621.6 0 214 70 134 621.6 0 134 71 726 621.6 0 726 72 108 621.6 0 108 73 125 621.6 0 125 74 74 621.6 0 74 75 55 621.6 0 55 76 42 621.6 0 42 77 45 621.6 0 45 78 40 621.6 0 40 79 75 621.6 0 75 80 84 621.6 0 84 81 271 621.6 0 271 82 146 621.6 0 146 83 124 621.6 0 124 84 471 621.6 0 471 85 306 621.6 0 306 86 344 621.6 0 344 87 64 621.6 0 64 88 57 621.6 0 57 89 55 621.6 0 55 90 129 621.6 0 129 91 79 621.6 0 79 92 63 621.6 0 63 93 52 621.6 0 52 94 63 621.6 0 63 95 55 621.6 0 55 96 51 621.6 0 51 97 83 621.6 0 83 98 65 621.6 0 65 99 66 621.6 0 66 100 74 621.6 0 74 101 73 621.6 0 73 102 67 621.6 0 67 103 43 621.6 0 43 104 49 621.6 0 49 105 49 621.6 0 49 106 57 621.6 0 57 107 78 621.6 0 78 108 57 621.6 0 57 109 50 621.6 0 50 110 204 621.6 0 204 111 231 621.6 0 231 112 211 621.6 0 211 113 57 621.6 0 57 114 55 621.6 0 55 115 32 621.6 0 32 116 48 621.6 0 48 117 41 621.6 0 41 118 38 621.6 0 38 119 40 621.6 0 40 120 48 621.6 0 48 121 53 621.6 0 53 122 57 621.6 0 57 123 54 621.6 0 54 124 54 621.6 0 54 125 58 621.6 0 58 126 44 621.6 0 44 127 38 621.6 0 38 128 49 621.6 0 49 129 50 621.6 0 50 130 155 621.6 0 155 131 71 621.6 0 71 132 83 621.6 0 83 133 66 621.6 0 66 134 48 621.6 0 48 135 42 621.6 0 42 136 45 621.6 0 45 137 55 621.6 0 55 138 73 621.6 0 73 139 62 621.6 0 62 140 58 621.6 0 58 141 69 621.6 0 69 142 72 621.6 0 72 143 81 621.6 0 81 144 66 621.6 0 66 145 113 621.6 0 113 146 132 621.6 0 132 147 86 621.6 0 86 148 129 621.6 0 129 149 112 621.6 0 112 150 125 621.6 0 125 151 130 621.6 0 130 152 89 621.6 0 89 153 70 621.6 0 70 154 67 621.6 0 67 155 61 621.6 0 61 156 295 621.6 0 295 157 210 621.6 0 210 158 120 621.6 0 120 159 89 621.6 0 89 160 52 621.6 0 52 161 88 621.6 0 88 162 47 621.6 0 47 163 45 621.6 0 45 164 48 621.6 0 48 165 46 621.6 0 46 166 61 621.6 0 61 167 56 621.6 0 56 168 126 621.6 0 126 169 128 621.6 0 128 170 162 621.6 0 162 171 82 621.6 0 82 172 139 621.6 0 139 173 57 621.6 0 57 174 76 621.6 0 76 175 81 621.6 0 81 176 282 621.6 0 282 177 155 621.6 0 155 178 273 621.6 0 273 179 46 621.6 0 46 180 59 621.6 0 59 181 99 621.6 0 99 182 69 621.6 0 69 183 54 621.6 0 54 184 101 621.6 0 101 185 52 621.6 0 52 186 114 621.6 0 114 187 162 621.6 0 162 188 154 621.6 0 154 189 153 621.6 0 153 190 210 621.6 0 210 191 95 621.6 0 95 192 60 621.6 0 60 193 65 621.6 0 65 194 92 621.6 0 92 195 116 621.6 0 116 196 172 621.6 0 172 197 562 621.6 0 562 198 587 621.6 0 587 199 591 621.6 0 591 200 76 621.6 0 76 201 78 621.6 0 78 202 235 621.6 0 235 203 274 621.6 0 274 204 492 621.6 0 492 205 269 621.6 0 269 206 152 621.6 0 152 207 56 621.6 0 56 208 59 621.6 0 59 209 93 621.6 0 93 210 124 621.6 0 124 211 104 621.6 0 104 212 116 621.6 0 116 213 227 621.6 0 227 214 204 621.6 0 204 215 175 621.6 0 175 216 86 621.6 0 86 217 71 621.6 0 71 218 86 621.6 0 86 219 92 621.6 0 92 220 68 621.6 0 68 221 67 621.6 0 67 222 163 621.6 0 163 223 557 621.6 0 557 224 622 621.6 0 622 225 549 621.6 0 549 226 74 621.6 0 74 227 55 621.6 0 55 228 71 621.6 0 71 229 55 621.6 0 55 230 51 621.6 0 51 231 58 621.6 0 58 232 46 621.6 0 46 233 70 621.6 0 70 234 85 621.6 0 85 235 92 621.6 0 92 236 64 621.6 0 64 237 53 621.6 0 53 238 64 621.6 0 64 239 103 621.6 0 103 240 89 621.6 0 89 241 168 621.6 0 168 242 142 621.6 0 142 243 135 621.6 0 135 244 142 621.6 0 142 245 114 621.6 0 114 246 114 621.6 0 114 247 83 621.6 0 83 248 89 621.6 0 89 249 86 621.6 0 86 250 64 621.6 0 64 251 82 621.6 0 82 252 59 621.6 0 59 253 62 621.6 0 62 254 64 621.6 0 64 255 89 621.6 0 89 256 83 621.6 0 83 257 179 621.6 0 179 258 340 621.6 0 340 259 157 621.6 0 157 260 222 621.6 0 222 261 136 621.6 0 136 262 64 621.6 0 64 263 71 621.6 0 71 264 50 621.6 0 50 265 62 621.6 0 62 266 51 621.6 0 51 267 54 621.6 0 54 268 57 621.6 0 57 269 79 621.6 0 79 270 102 621.6 0 102 271 241 621.6 0 241 272 191 621.6 0 191 273 166 621.6 0 166 274 59 621.6 0 59 275 68 621.6 0 68 276 76 621.6 0 76 277 135 621.6 0 135 278 100 621.6 0 100 279 134 621.6 0 134 280 85 621.6 0 85 281 95 621.6 0 95 282 87 621.6 0 87 283 100 621.6 0 100 284 160 621.6 0 160 285 126 621.6 0 126 286 107 621.6 0 107 287 105 621.6 0 105 288 103 621.6 0 103 289 82 621.6 0 82 290 56 621.6 0 56 291 83 621.6 0 83 292 92 621.6 0 92 293 88 621.6 0 88 294 112 621.6 0 112 295 108 621.6 0 108 296 125 621.6 0 125 297 210 621.6 0 210 298 365 621.6 0 365 299 424 621.6 0 424 300 232 621.6 0 232 301 156 621.6 0 156 Finished in 1.16 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 27,374 Reads with adapters: 4,630 (16.9%) Reads that were too short: 72 (0.3%) Reads written (passing filters): 4,558 (16.7%) Total basepairs processed: 5,963,600 bp Total written (filtered): 1,252,210 bp (21.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4630 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 4 0.0 3 0 0 2 2 16 34 0.0 3 0 26 5 3 17 4397 0.0 3 4018 324 44 11 18 15 0.0 3 0 11 1 3 19 26 0.0 3 4 1 15 6 20 17 0.0 3 0 0 1 16 23 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 29 4 0.0 3 0 0 1 3 30 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 0 2 32 1 0.0 3 0 0 0 1 35 4 0.0 3 0 0 0 4 38 1 0.0 3 0 0 0 1 41 11 0.0 3 0 0 0 11 44 2 0.0 3 0 0 0 2 47 1 0.0 3 0 1 50 2 0.0 3 1 0 0 1 55 1 0.0 3 0 0 0 1 60 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 70 1 0.0 3 0 0 1 73 1 0.0 3 0 1 74 1 0.0 3 0 0 1 80 5 0.0 3 0 0 1 4 81 8 0.0 3 0 0 0 8 82 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 87 1 0.0 3 0 0 0 1 89 3 0.0 3 0 0 0 3 92 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 95 1 0.0 3 0 1 100 1 0.0 3 0 0 0 1 101 2 0.0 3 0 0 0 2 102 1 0.0 3 0 0 0 1 105 3 0.0 3 0 0 0 3 107 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 110 2 0.0 3 0 0 0 2 111 2 0.0 3 0 0 0 2 112 2 0.0 3 0 0 0 2 113 2 0.0 3 0 0 0 2 114 2 0.0 3 1 0 0 1 117 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 128 1 0.0 3 0 0 0 1 131 1 0.0 3 0 0 0 1 133 5 0.0 3 0 0 0 5 136 1 0.0 3 0 0 0 1 137 4 0.0 3 0 0 0 4 138 1 0.0 3 0 0 0 1 140 1 0.0 3 0 0 0 1 142 2 0.0 3 0 0 0 2 143 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 1 163 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 0 1 167 1 0.0 3 0 0 0 1 178 1 0.0 3 0 0 0 1 181 1 0.0 3 0 0 0 1 184 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 193 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 201 1 0.0 3 0 0 0 1 209 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 225 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 244 2 0.0 3 0 0 0 2 255 1 0.0 3 0 0 0 1 258 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 1 279 1 0.0 3 0 0 0 1 281 2 0.0 3 0 0 0 2 284 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.17 s (37 us/read; 1.61 M reads/minute). === Summary === Total reads processed: 4,558 Reads with adapters: 18 (0.4%) Reads written (passing filters): 4,558 (100.0%) Total basepairs processed: 1,252,210 bp Total written (filtered): 1,252,156 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 18 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 22.2% C: 61.1% G: 16.7% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 18 71.2 0 18