This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTTGCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.26 s (26 us/read; 2.31 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 31,622 (1.2%) Reads that were too short: 7,714 (0.3%) Reads written (passing filters): 23,908 (0.9%) Total basepairs processed: 766,381,518 bp Total written (filtered): 5,516,776 bp (0.7%) === Adapter 1 === Sequence: GTTGCC; Type: regular 5'; Length: 6; Trimmed: 31622 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 211 621.6 0 211 7 4114 621.6 0 4114 8 4860 621.6 0 4860 9 59 621.6 0 59 10 64 621.6 0 64 11 82 621.6 0 82 12 53 621.6 0 53 13 40 621.6 0 40 14 95 621.6 0 95 15 55 621.6 0 55 16 59 621.6 0 59 17 51 621.6 0 51 18 48 621.6 0 48 19 59 621.6 0 59 20 44 621.6 0 44 21 53 621.6 0 53 22 47 621.6 0 47 23 61 621.6 0 61 24 56 621.6 0 56 25 57 621.6 0 57 26 41 621.6 0 41 27 52 621.6 0 52 28 38 621.6 0 38 29 52 621.6 0 52 30 52 621.6 0 52 31 44 621.6 0 44 32 30 621.6 0 30 33 27 621.6 0 27 34 49 621.6 0 49 35 24 621.6 0 24 36 59 621.6 0 59 37 53 621.6 0 53 38 57 621.6 0 57 39 49 621.6 0 49 40 44 621.6 0 44 41 51 621.6 0 51 42 63 621.6 0 63 43 58 621.6 0 58 44 47 621.6 0 47 45 49 621.6 0 49 46 56 621.6 0 56 47 26 621.6 0 26 48 54 621.6 0 54 49 57 621.6 0 57 50 50 621.6 0 50 51 59 621.6 0 59 52 56 621.6 0 56 53 49 621.6 0 49 54 54 621.6 0 54 55 70 621.6 0 70 56 73 621.6 0 73 57 56 621.6 0 56 58 55 621.6 0 55 59 37 621.6 0 37 60 61 621.6 0 61 61 50 621.6 0 50 62 47 621.6 0 47 63 43 621.6 0 43 64 56 621.6 0 56 65 58 621.6 0 58 66 51 621.6 0 51 67 48 621.6 0 48 68 60 621.6 0 60 69 249 621.6 0 249 70 115 621.6 0 115 71 1115 621.6 0 1115 72 133 621.6 0 133 73 114 621.6 0 114 74 63 621.6 0 63 75 80 621.6 0 80 76 58 621.6 0 58 77 43 621.6 0 43 78 52 621.6 0 52 79 43 621.6 0 43 80 67 621.6 0 67 81 241 621.6 0 241 82 95 621.6 0 95 83 58 621.6 0 58 84 303 621.6 0 303 85 223 621.6 0 223 86 322 621.6 0 322 87 36 621.6 0 36 88 40 621.6 0 40 89 47 621.6 0 47 90 42 621.6 0 42 91 41 621.6 0 41 92 39 621.6 0 39 93 46 621.6 0 46 94 63 621.6 0 63 95 66 621.6 0 66 96 57 621.6 0 57 97 58 621.6 0 58 98 37 621.6 0 37 99 64 621.6 0 64 100 62 621.6 0 62 101 50 621.6 0 50 102 57 621.6 0 57 103 33 621.6 0 33 104 37 621.6 0 37 105 53 621.6 0 53 106 59 621.6 0 59 107 66 621.6 0 66 108 56 621.6 0 56 109 57 621.6 0 57 110 174 621.6 0 174 111 230 621.6 0 230 112 204 621.6 0 204 113 48 621.6 0 48 114 36 621.6 0 36 115 30 621.6 0 30 116 49 621.6 0 49 117 42 621.6 0 42 118 38 621.6 0 38 119 35 621.6 0 35 120 64 621.6 0 64 121 48 621.6 0 48 122 56 621.6 0 56 123 41 621.6 0 41 124 55 621.6 0 55 125 57 621.6 0 57 126 42 621.6 0 42 127 35 621.6 0 35 128 51 621.6 0 51 129 53 621.6 0 53 130 151 621.6 0 151 131 74 621.6 0 74 132 50 621.6 0 50 133 59 621.6 0 59 134 38 621.6 0 38 135 54 621.6 0 54 136 55 621.6 0 55 137 55 621.6 0 55 138 50 621.6 0 50 139 55 621.6 0 55 140 50 621.6 0 50 141 52 621.6 0 52 142 52 621.6 0 52 143 72 621.6 0 72 144 42 621.6 0 42 145 62 621.6 0 62 146 90 621.6 0 90 147 80 621.6 0 80 148 95 621.6 0 95 149 101 621.6 0 101 150 91 621.6 0 91 151 60 621.6 0 60 152 62 621.6 0 62 153 50 621.6 0 50 154 64 621.6 0 64 155 55 621.6 0 55 156 134 621.6 0 134 157 105 621.6 0 105 158 88 621.6 0 88 159 60 621.6 0 60 160 42 621.6 0 42 161 88 621.6 0 88 162 36 621.6 0 36 163 51 621.6 0 51 164 46 621.6 0 46 165 45 621.6 0 45 166 59 621.6 0 59 167 45 621.6 0 45 168 102 621.6 0 102 169 116 621.6 0 116 170 130 621.6 0 130 171 88 621.6 0 88 172 119 621.6 0 119 173 47 621.6 0 47 174 57 621.6 0 57 175 54 621.6 0 54 176 123 621.6 0 123 177 70 621.6 0 70 178 101 621.6 0 101 179 45 621.6 0 45 180 56 621.6 0 56 181 48 621.6 0 48 182 57 621.6 0 57 183 50 621.6 0 50 184 63 621.6 0 63 185 44 621.6 0 44 186 67 621.6 0 67 187 70 621.6 0 70 188 101 621.6 0 101 189 74 621.6 0 74 190 98 621.6 0 98 191 69 621.6 0 69 192 56 621.6 0 56 193 59 621.6 0 59 194 72 621.6 0 72 195 58 621.6 0 58 196 104 621.6 0 104 197 276 621.6 0 276 198 286 621.6 0 286 199 287 621.6 0 287 200 43 621.6 0 43 201 39 621.6 0 39 202 104 621.6 0 104 203 138 621.6 0 138 204 183 621.6 0 183 205 113 621.6 0 113 206 69 621.6 0 69 207 55 621.6 0 55 208 41 621.6 0 41 209 72 621.6 0 72 210 53 621.6 0 53 211 58 621.6 0 58 212 56 621.6 0 56 213 58 621.6 0 58 214 69 621.6 0 69 215 70 621.6 0 70 216 40 621.6 0 40 217 62 621.6 0 62 218 50 621.6 0 50 219 59 621.6 0 59 220 52 621.6 0 52 221 46 621.6 0 46 222 78 621.6 0 78 223 257 621.6 0 257 224 292 621.6 0 292 225 282 621.6 0 282 226 60 621.6 0 60 227 52 621.6 0 52 228 77 621.6 0 77 229 45 621.6 0 45 230 48 621.6 0 48 231 50 621.6 0 50 232 51 621.6 0 51 233 56 621.6 0 56 234 61 621.6 0 61 235 56 621.6 0 56 236 68 621.6 0 68 237 47 621.6 0 47 238 45 621.6 0 45 239 79 621.6 0 79 240 77 621.6 0 77 241 59 621.6 0 59 242 61 621.6 0 61 243 76 621.6 0 76 244 66 621.6 0 66 245 64 621.6 0 64 246 64 621.6 0 64 247 62 621.6 0 62 248 77 621.6 0 77 249 63 621.6 0 63 250 83 621.6 0 83 251 56 621.6 0 56 252 48 621.6 0 48 253 52 621.6 0 52 254 73 621.6 0 73 255 65 621.6 0 65 256 67 621.6 0 67 257 76 621.6 0 76 258 181 621.6 0 181 259 90 621.6 0 90 260 163 621.6 0 163 261 98 621.6 0 98 262 59 621.6 0 59 263 64 621.6 0 64 264 41 621.6 0 41 265 46 621.6 0 46 266 36 621.6 0 36 267 46 621.6 0 46 268 54 621.6 0 54 269 50 621.6 0 50 270 61 621.6 0 61 271 102 621.6 0 102 272 105 621.6 0 105 273 84 621.6 0 84 274 56 621.6 0 56 275 52 621.6 0 52 276 56 621.6 0 56 277 76 621.6 0 76 278 47 621.6 0 47 279 92 621.6 0 92 280 57 621.6 0 57 281 61 621.6 0 61 282 61 621.6 0 61 283 74 621.6 0 74 284 95 621.6 0 95 285 59 621.6 0 59 286 67 621.6 0 67 287 66 621.6 0 66 288 76 621.6 0 76 289 48 621.6 0 48 290 38 621.6 0 38 291 61 621.6 0 61 292 54 621.6 0 54 293 50 621.6 0 50 294 73 621.6 0 73 295 61 621.6 0 61 296 49 621.6 0 49 297 99 621.6 0 99 298 170 621.6 0 170 299 198 621.6 0 198 300 123 621.6 0 123 301 74 621.6 0 74 Finished in 1.17 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 23,908 Reads with adapters: 5,091 (21.3%) Reads that were too short: 0 (0.0%) Reads written (passing filters): 5,091 (21.3%) Total basepairs processed: 5,516,776 bp Total written (filtered): 1,390,071 bp (25.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 5091 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 91 0.0 3 0 89 2 20 4987 0.0 4 4903 80 4 21 6 0.0 4 0 6 23 4 0.0 4 0 0 0 4 73 1 0.0 4 1 127 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.19 s (37 us/read; 1.61 M reads/minute). === Summary === Total reads processed: 5,091 Reads with adapters: 529 (10.4%) Reads written (passing filters): 5,091 (100.0%) Total basepairs processed: 1,390,071 bp Total written (filtered): 1,383,872 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 529 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.7% C: 3.2% G: 93.2% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 6 79.5 0 6 9 2 0.0 0 0 2 10 68 0.0 1 1 67 11 449 0.0 1 1 448 14 1 0.0 1 0 1 151 1 0.0 1 0 1 170 1 0.0 1 1 209 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.