This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TACTTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.80 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 307,202 (12.1%) Reads that were too short: 26,107 (1.0%) Reads written (passing filters): 281,095 (11.0%) Total basepairs processed: 766,381,518 bp Total written (filtered): 54,616,683 bp (7.1%) === Adapter 1 === Sequence: TACTTC; Type: regular 5'; Length: 6; Trimmed: 307202 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 5731 621.6 0 5731 7 35932 621.6 0 35932 8 454 621.6 0 454 9 35109 621.6 0 35109 10 54 621.6 0 54 11 38 621.6 0 38 12 75 621.6 0 75 13 37 621.6 0 37 14 37 621.6 0 37 15 37 621.6 0 37 16 62 621.6 0 62 17 66 621.6 0 66 18 53 621.6 0 53 19 49 621.6 0 49 20 38 621.6 0 38 21 52 621.6 0 52 22 47 621.6 0 47 23 50 621.6 0 50 24 65 621.6 0 65 25 64 621.6 0 64 26 59 621.6 0 59 27 42 621.6 0 42 28 50 621.6 0 50 29 40 621.6 0 40 30 32 621.6 0 32 31 74 621.6 0 74 32 49 621.6 0 49 33 41 621.6 0 41 34 42 621.6 0 42 35 42 621.6 0 42 36 54 621.6 0 54 37 49 621.6 0 49 38 56 621.6 0 56 39 46 621.6 0 46 40 55 621.6 0 55 41 42 621.6 0 42 42 59 621.6 0 59 43 65 621.6 0 65 44 55 621.6 0 55 45 57 621.6 0 57 46 58 621.6 0 58 47 52 621.6 0 52 48 56 621.6 0 56 49 43 621.6 0 43 50 49 621.6 0 49 51 52 621.6 0 52 52 47 621.6 0 47 53 48 621.6 0 48 54 38 621.6 0 38 55 41 621.6 0 41 56 106 621.6 0 106 57 52 621.6 0 52 58 82 621.6 0 82 59 66 621.6 0 66 60 43 621.6 0 43 61 47 621.6 0 47 62 58 621.6 0 58 63 62 621.6 0 62 64 57 621.6 0 57 65 42 621.6 0 42 66 68 621.6 0 68 67 56 621.6 0 56 68 50 621.6 0 50 69 53 621.6 0 53 70 64 621.6 0 64 71 179 621.6 0 179 72 76 621.6 0 76 73 222 621.6 0 222 74 61 621.6 0 61 75 81 621.6 0 81 76 62 621.6 0 62 77 51 621.6 0 51 78 56 621.6 0 56 79 77 621.6 0 77 80 48 621.6 0 48 81 58 621.6 0 58 82 72 621.6 0 72 83 63 621.6 0 63 84 51 621.6 0 51 85 76 621.6 0 76 86 65 621.6 0 65 87 118 621.6 0 118 88 101 621.6 0 101 89 162 621.6 0 162 90 100 621.6 0 100 91 108 621.6 0 108 92 58 621.6 0 58 93 59 621.6 0 59 94 73 621.6 0 73 95 58 621.6 0 58 96 53 621.6 0 53 97 72 621.6 0 72 98 79 621.6 0 79 99 40 621.6 0 40 100 65 621.6 0 65 101 76 621.6 0 76 102 88 621.6 0 88 103 168 621.6 0 168 104 243 621.6 0 243 105 170 621.6 0 170 106 143 621.6 0 143 107 100 621.6 0 100 108 75 621.6 0 75 109 91 621.6 0 91 110 85 621.6 0 85 111 97 621.6 0 97 112 121 621.6 0 121 113 108 621.6 0 108 114 71 621.6 0 71 115 136 621.6 0 136 116 293 621.6 0 293 117 96 621.6 0 96 118 118 621.6 0 118 119 710 621.6 0 710 120 81 621.6 0 81 121 82 621.6 0 82 122 61 621.6 0 61 123 84 621.6 0 84 124 69 621.6 0 69 125 77 621.6 0 77 126 130 621.6 0 130 127 144 621.6 0 144 128 352 621.6 0 352 129 952 621.6 0 952 130 201 621.6 0 201 131 334 621.6 0 334 132 288 621.6 0 288 133 234 621.6 0 234 134 968 621.6 0 968 135 550 621.6 0 550 136 480 621.6 0 480 137 136 621.6 0 136 138 165 621.6 0 165 139 350 621.6 0 350 140 511 621.6 0 511 141 603 621.6 0 603 142 873 621.6 0 873 143 784 621.6 0 784 144 2007 621.6 0 2007 145 5547 621.6 0 5547 146 49439 621.6 0 49439 147 66795 621.6 0 66795 148 49235 621.6 0 49235 149 462 621.6 0 462 150 3076 621.6 0 3076 151 954 621.6 0 954 152 5339 621.6 0 5339 153 86 621.6 0 86 154 54 621.6 0 54 155 81 621.6 0 81 156 306 621.6 0 306 157 267 621.6 0 267 158 383 621.6 0 383 159 53 621.6 0 53 160 101 621.6 0 101 161 104 621.6 0 104 162 87 621.6 0 87 163 67 621.6 0 67 164 62 621.6 0 62 165 70 621.6 0 70 166 47 621.6 0 47 167 58 621.6 0 58 168 58 621.6 0 58 169 47 621.6 0 47 170 47 621.6 0 47 171 32 621.6 0 32 172 42 621.6 0 42 173 45 621.6 0 45 174 47 621.6 0 47 175 65 621.6 0 65 176 73 621.6 0 73 177 58 621.6 0 58 178 47 621.6 0 47 179 52 621.6 0 52 180 68 621.6 0 68 181 94 621.6 0 94 182 108 621.6 0 108 183 83 621.6 0 83 184 57 621.6 0 57 185 66 621.6 0 66 186 96 621.6 0 96 187 70 621.6 0 70 188 79 621.6 0 79 189 40 621.6 0 40 190 65 621.6 0 65 191 42 621.6 0 42 192 54 621.6 0 54 193 56 621.6 0 56 194 43 621.6 0 43 195 58 621.6 0 58 196 54 621.6 0 54 197 52 621.6 0 52 198 45 621.6 0 45 199 47 621.6 0 47 200 69 621.6 0 69 201 65 621.6 0 65 202 80 621.6 0 80 203 95 621.6 0 95 204 101 621.6 0 101 205 71 621.6 0 71 206 68 621.6 0 68 207 89 621.6 0 89 208 108 621.6 0 108 209 81 621.6 0 81 210 83 621.6 0 83 211 97 621.6 0 97 212 229 621.6 0 229 213 133 621.6 0 133 214 125 621.6 0 125 215 70 621.6 0 70 216 70 621.6 0 70 217 58 621.6 0 58 218 68 621.6 0 68 219 60 621.6 0 60 220 168 621.6 0 168 221 115 621.6 0 115 222 114 621.6 0 114 223 122 621.6 0 122 224 76 621.6 0 76 225 82 621.6 0 82 226 129 621.6 0 129 227 91 621.6 0 91 228 79 621.6 0 79 229 86 621.6 0 86 230 92 621.6 0 92 231 92 621.6 0 92 232 65 621.6 0 65 233 94 621.6 0 94 234 108 621.6 0 108 235 161 621.6 0 161 236 92 621.6 0 92 237 71 621.6 0 71 238 84 621.6 0 84 239 101 621.6 0 101 240 87 621.6 0 87 241 88 621.6 0 88 242 89 621.6 0 89 243 93 621.6 0 93 244 107 621.6 0 107 245 88 621.6 0 88 246 118 621.6 0 118 247 98 621.6 0 98 248 65 621.6 0 65 249 97 621.6 0 97 250 112 621.6 0 112 251 134 621.6 0 134 252 150 621.6 0 150 253 188 621.6 0 188 254 298 621.6 0 298 255 529 621.6 0 529 256 4046 621.6 0 4046 257 4451 621.6 0 4451 258 5838 621.6 0 5838 259 928 621.6 0 928 260 114 621.6 0 114 261 89 621.6 0 89 262 205 621.6 0 205 263 255 621.6 0 255 264 294 621.6 0 294 265 91 621.6 0 91 266 103 621.6 0 103 267 105 621.6 0 105 268 140 621.6 0 140 269 111 621.6 0 111 270 58 621.6 0 58 271 75 621.6 0 75 272 60 621.6 0 60 273 61 621.6 0 61 274 78 621.6 0 78 275 92 621.6 0 92 276 69 621.6 0 69 277 62 621.6 0 62 278 74 621.6 0 74 279 152 621.6 0 152 280 276 621.6 0 276 281 253 621.6 0 253 282 253 621.6 0 253 283 122 621.6 0 122 284 123 621.6 0 123 285 115 621.6 0 115 286 96 621.6 0 96 287 77 621.6 0 77 288 77 621.6 0 77 289 116 621.6 0 116 290 75 621.6 0 75 291 55 621.6 0 55 292 44 621.6 0 44 293 87 621.6 0 87 294 102 621.6 0 102 295 82 621.6 0 82 296 84 621.6 0 84 297 64 621.6 0 64 298 80 621.6 0 80 299 62 621.6 0 62 300 89 621.6 0 89 301 175 621.6 0 175 Finished in 11.13 s (40 us/read; 1.52 M reads/minute). === Summary === Total reads processed: 281,095 Reads with adapters: 41,358 (14.7%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 41,347 (14.7%) Total basepairs processed: 54,616,683 bp Total written (filtered): 11,312,809 bp (20.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 41358 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 17 0.0 3 0 0 17 19 420 0.0 3 0 410 10 20 40568 0.0 4 39935 608 21 4 21 91 0.0 4 16 73 2 22 12 0.0 4 2 1 9 23 46 0.0 4 27 0 0 15 4 28 1 0.0 4 0 0 0 0 1 34 8 0.0 4 0 0 0 0 8 71 2 0.0 4 2 74 1 0.0 4 0 0 0 0 1 75 1 0.0 4 1 77 1 0.0 4 1 79 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 167 0.0 4 0 0 0 0 167 129 1 0.0 4 0 0 0 0 1 135 9 0.0 4 0 0 0 0 9 141 1 0.0 4 0 0 0 0 1 195 1 0.0 4 1 216 1 0.0 4 0 1 228 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 234 1 0.0 4 0 0 0 0 1 281 1 0.0 4 0 0 0 0 1 287 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.43 s (35 us/read; 1.73 M reads/minute). === Summary === Total reads processed: 41,347 Reads with adapters: 1,079 (2.6%) Reads written (passing filters): 41,347 (100.0%) Total basepairs processed: 11,312,809 bp Total written (filtered): 11,247,168 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1079 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.7% C: 5.4% G: 68.6% T: 16.4% none/other: 4.0% Overview of removed sequences length count expect max.err error counts 3 73 646.0 0 73 4 8 161.5 0 8 5 4 40.4 0 4 8 1 0.6 0 1 9 15 0.2 0 3 12 10 16 0.0 1 0 16 11 702 0.0 1 5 697 12 42 0.0 1 2 40 13 3 0.0 1 1 2 15 4 0.0 1 0 4 16 1 0.0 1 0 1 148 1 0.0 1 1 152 1 0.0 1 0 1 163 1 0.0 1 1 222 1 0.0 1 1 269 1 0.0 1 0 1 271 117 0.0 1 111 6 272 17 0.0 1 16 1 273 25 0.0 1 25 274 34 0.0 1 33 1 275 12 0.0 1 12