This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCTCCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 66.71 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 77,741 (3.1%) Reads that were too short: 19,872 (0.8%) Reads written (passing filters): 57,869 (2.3%) Total basepairs processed: 766,381,518 bp Total written (filtered): 10,547,058 bp (1.4%) === Adapter 1 === Sequence: GCTCCG; Type: regular 5'; Length: 6; Trimmed: 77741 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2006 621.6 0 2006 7 5794 621.6 0 5794 8 303 621.6 0 303 9 4687 621.6 0 4687 10 57 621.6 0 57 11 65 621.6 0 65 12 29 621.6 0 29 13 40 621.6 0 40 14 29 621.6 0 29 15 70 621.6 0 70 16 31 621.6 0 31 17 37 621.6 0 37 18 27 621.6 0 27 19 25 621.6 0 25 20 35 621.6 0 35 21 33 621.6 0 33 22 55 621.6 0 55 23 40 621.6 0 40 24 35 621.6 0 35 25 26 621.6 0 26 26 39 621.6 0 39 27 33 621.6 0 33 28 37 621.6 0 37 29 35 621.6 0 35 30 56 621.6 0 56 31 25 621.6 0 25 32 38 621.6 0 38 33 51 621.6 0 51 34 27 621.6 0 27 35 58 621.6 0 58 36 40 621.6 0 40 37 36 621.6 0 36 38 65 621.6 0 65 39 39 621.6 0 39 40 138 621.6 0 138 41 73 621.6 0 73 42 101 621.6 0 101 43 32 621.6 0 32 44 29 621.6 0 29 45 54 621.6 0 54 46 30 621.6 0 30 47 40 621.6 0 40 48 31 621.6 0 31 49 25 621.6 0 25 50 31 621.6 0 31 51 30 621.6 0 30 52 35 621.6 0 35 53 36 621.6 0 36 54 34 621.6 0 34 55 159 621.6 0 159 56 92 621.6 0 92 57 141 621.6 0 141 58 51 621.6 0 51 59 57 621.6 0 57 60 98 621.6 0 98 61 47 621.6 0 47 62 64 621.6 0 64 63 43 621.6 0 43 64 52 621.6 0 52 65 38 621.6 0 38 66 81 621.6 0 81 67 34 621.6 0 34 68 86 621.6 0 86 69 39 621.6 0 39 70 33 621.6 0 33 71 84 621.6 0 84 72 35 621.6 0 35 73 45 621.6 0 45 74 32 621.6 0 32 75 79 621.6 0 79 76 37 621.6 0 37 77 30 621.6 0 30 78 28 621.6 0 28 79 41 621.6 0 41 80 23 621.6 0 23 81 34 621.6 0 34 82 28 621.6 0 28 83 50 621.6 0 50 84 77 621.6 0 77 85 48 621.6 0 48 86 34 621.6 0 34 87 29 621.6 0 29 88 43 621.6 0 43 89 60 621.6 0 60 90 63 621.6 0 63 91 65 621.6 0 65 92 48 621.6 0 48 93 57 621.6 0 57 94 46 621.6 0 46 95 51 621.6 0 51 96 38 621.6 0 38 97 40 621.6 0 40 98 35 621.6 0 35 99 22 621.6 0 22 100 24 621.6 0 24 101 28 621.6 0 28 102 57 621.6 0 57 103 100 621.6 0 100 104 96 621.6 0 96 105 392 621.6 0 392 106 284 621.6 0 284 107 417 621.6 0 417 108 28 621.6 0 28 109 45 621.6 0 45 110 47 621.6 0 47 111 60 621.6 0 60 112 64 621.6 0 64 113 84 621.6 0 84 114 91 621.6 0 91 115 91 621.6 0 91 116 47 621.6 0 47 117 76 621.6 0 76 118 53 621.6 0 53 119 37 621.6 0 37 120 58 621.6 0 58 121 49 621.6 0 49 122 51 621.6 0 51 123 57 621.6 0 57 124 52 621.6 0 52 125 80 621.6 0 80 126 117 621.6 0 117 127 282 621.6 0 282 128 892 621.6 0 892 129 390 621.6 0 390 130 611 621.6 0 611 131 121 621.6 0 121 132 138 621.6 0 138 133 114 621.6 0 114 134 181 621.6 0 181 135 98 621.6 0 98 136 103 621.6 0 103 137 181 621.6 0 181 138 83 621.6 0 83 139 88 621.6 0 88 140 94 621.6 0 94 141 109 621.6 0 109 142 211 621.6 0 211 143 127 621.6 0 127 144 212 621.6 0 212 145 232 621.6 0 232 146 291 621.6 0 291 147 4333 621.6 0 4333 148 3488 621.6 0 3488 149 8223 621.6 0 8223 150 131 621.6 0 131 151 71 621.6 0 71 152 239 621.6 0 239 153 182 621.6 0 182 154 455 621.6 0 455 155 116 621.6 0 116 156 119 621.6 0 119 157 96 621.6 0 96 158 73 621.6 0 73 159 82 621.6 0 82 160 61 621.6 0 61 161 64 621.6 0 64 162 65 621.6 0 65 163 55 621.6 0 55 164 61 621.6 0 61 165 43 621.6 0 43 166 42 621.6 0 42 167 41 621.6 0 41 168 93 621.6 0 93 169 147 621.6 0 147 170 198 621.6 0 198 171 107 621.6 0 107 172 394 621.6 0 394 173 289 621.6 0 289 174 267 621.6 0 267 175 66 621.6 0 66 176 59 621.6 0 59 177 70 621.6 0 70 178 101 621.6 0 101 179 72 621.6 0 72 180 63 621.6 0 63 181 45 621.6 0 45 182 88 621.6 0 88 183 151 621.6 0 151 184 147 621.6 0 147 185 113 621.6 0 113 186 175 621.6 0 175 187 415 621.6 0 415 188 2907 621.6 0 2907 189 2250 621.6 0 2250 190 3375 621.6 0 3375 191 115 621.6 0 115 192 52 621.6 0 52 193 94 621.6 0 94 194 42 621.6 0 42 195 75 621.6 0 75 196 150 621.6 0 150 197 133 621.6 0 133 198 184 621.6 0 184 199 257 621.6 0 257 200 486 621.6 0 486 201 2367 621.6 0 2367 202 1530 621.6 0 1530 203 2551 621.6 0 2551 204 296 621.6 0 296 205 79 621.6 0 79 206 89 621.6 0 89 207 44 621.6 0 44 208 80 621.6 0 80 209 51 621.6 0 51 210 65 621.6 0 65 211 34 621.6 0 34 212 40 621.6 0 40 213 65 621.6 0 65 214 70 621.6 0 70 215 80 621.6 0 80 216 52 621.6 0 52 217 34 621.6 0 34 218 28 621.6 0 28 219 35 621.6 0 35 220 29 621.6 0 29 221 55 621.6 0 55 222 56 621.6 0 56 223 54 621.6 0 54 224 74 621.6 0 74 225 98 621.6 0 98 226 149 621.6 0 149 227 137 621.6 0 137 228 248 621.6 0 248 229 945 621.6 0 945 230 217 621.6 0 217 231 115 621.6 0 115 232 75 621.6 0 75 233 43 621.6 0 43 234 58 621.6 0 58 235 48 621.6 0 48 236 66 621.6 0 66 237 51 621.6 0 51 238 45 621.6 0 45 239 51 621.6 0 51 240 51 621.6 0 51 241 75 621.6 0 75 242 56 621.6 0 56 243 70 621.6 0 70 244 58 621.6 0 58 245 88 621.6 0 88 246 65 621.6 0 65 247 82 621.6 0 82 248 92 621.6 0 92 249 128 621.6 0 128 250 158 621.6 0 158 251 119 621.6 0 119 252 163 621.6 0 163 253 89 621.6 0 89 254 59 621.6 0 59 255 54 621.6 0 54 256 29 621.6 0 29 257 58 621.6 0 58 258 139 621.6 0 139 259 96 621.6 0 96 260 332 621.6 0 332 261 248 621.6 0 248 262 177 621.6 0 177 263 93 621.6 0 93 264 70 621.6 0 70 265 84 621.6 0 84 266 83 621.6 0 83 267 87 621.6 0 87 268 86 621.6 0 86 269 655 621.6 0 655 270 257 621.6 0 257 271 383 621.6 0 383 272 147 621.6 0 147 273 90 621.6 0 90 274 107 621.6 0 107 275 123 621.6 0 123 276 164 621.6 0 164 277 142 621.6 0 142 278 131 621.6 0 131 279 167 621.6 0 167 280 362 621.6 0 362 281 251 621.6 0 251 282 644 621.6 0 644 283 249 621.6 0 249 284 230 621.6 0 230 285 78 621.6 0 78 286 82 621.6 0 82 287 135 621.6 0 135 288 79 621.6 0 79 289 123 621.6 0 123 290 126 621.6 0 126 291 491 621.6 0 491 292 306 621.6 0 306 293 689 621.6 0 689 294 714 621.6 0 714 295 623 621.6 0 623 296 759 621.6 0 759 297 248 621.6 0 248 298 84 621.6 0 84 299 145 621.6 0 145 300 136 621.6 0 136 301 126 621.6 0 126 Finished in 2.19 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 57,869 Reads with adapters: 6,780 (11.7%) Reads that were too short: 125 (0.2%) Reads written (passing filters): 6,655 (11.5%) Total basepairs processed: 10,547,058 bp Total written (filtered): 1,801,191 bp (17.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6780 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 4 0.0 3 0 0 2 2 16 59 0.0 3 0 50 4 5 17 4795 0.0 3 4419 315 46 15 18 54 0.0 3 3 33 15 3 19 1538 0.0 3 1382 53 20 83 20 4 0.0 3 0 2 1 1 21 7 0.0 3 0 0 3 4 22 16 0.0 3 0 0 0 16 23 1 0.0 3 0 0 0 1 27 3 0.0 3 0 0 0 3 40 1 0.0 3 0 0 0 1 43 22 0.0 3 0 0 0 22 45 47 0.0 3 0 0 2 45 46 3 0.0 3 0 0 0 3 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 56 10 0.0 3 0 0 0 10 57 3 0.0 3 0 0 0 3 59 1 0.0 3 0 0 0 1 60 2 0.0 3 0 0 0 2 61 2 0.0 3 0 0 0 2 63 35 0.0 3 0 0 0 35 64 10 0.0 3 0 0 0 10 66 5 0.0 3 0 0 0 5 67 1 0.0 3 0 0 0 1 70 8 0.0 3 0 0 0 8 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 79 33 0.0 3 0 0 0 33 80 1 0.0 3 0 0 0 1 82 1 0.0 3 0 0 0 1 85 2 0.0 3 0 0 0 2 86 14 0.0 3 0 0 0 14 87 9 0.0 3 0 0 0 9 88 1 0.0 3 0 0 0 1 90 3 0.0 3 0 0 0 3 97 1 0.0 3 0 0 0 1 99 3 0.0 3 0 0 0 3 100 8 0.0 3 0 0 0 8 101 2 0.0 3 0 0 0 2 105 3 0.0 3 0 0 0 3 107 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 116 2 0.0 3 0 0 0 2 118 1 0.0 3 0 0 0 1 120 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 123 2 0.0 3 0 0 0 2 124 2 0.0 3 0 0 0 2 127 1 0.0 3 0 0 0 1 131 5 0.0 3 0 0 0 5 132 1 0.0 3 0 0 0 1 133 2 0.0 3 0 0 0 2 134 2 0.0 3 0 0 0 2 138 2 0.0 3 0 0 0 2 139 2 0.0 3 0 0 0 2 140 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 151 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 164 2 0.0 3 0 0 0 2 165 6 0.0 3 0 0 0 6 172 1 0.0 3 0 0 0 1 174 2 0.0 3 0 0 0 2 181 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 199 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 218 1 0.0 3 0 0 0 1 228 1 0.0 3 0 0 0 1 280 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.23 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 6,655 Reads with adapters: 47 (0.7%) Reads written (passing filters): 6,655 (100.0%) Total basepairs processed: 1,801,191 bp Total written (filtered): 1,801,009 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 47 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 34.0% C: 31.9% G: 29.8% T: 4.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 29 104.0 0 29 4 6 26.0 0 6 5 1 6.5 0 1 6 11 1.6 0 11