This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCTCCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.35 s (26 us/read; 2.27 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 58,539 (2.3%) Reads that were too short: 10,554 (0.4%) Reads written (passing filters): 47,985 (1.9%) Total basepairs processed: 766,381,518 bp Total written (filtered): 9,119,936 bp (1.2%) === Adapter 1 === Sequence: GCTCCG; Type: regular 5'; Length: 6; Trimmed: 58539 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1929 621.6 0 1929 7 6195 621.6 0 6195 8 246 621.6 0 246 9 5423 621.6 0 5423 10 26 621.6 0 26 11 25 621.6 0 25 12 21 621.6 0 21 13 18 621.6 0 18 14 17 621.6 0 17 15 26 621.6 0 26 16 12 621.6 0 12 17 20 621.6 0 20 18 13 621.6 0 13 19 21 621.6 0 21 20 13 621.6 0 13 21 19 621.6 0 19 22 21 621.6 0 21 23 16 621.6 0 16 24 13 621.6 0 13 25 16 621.6 0 16 26 24 621.6 0 24 27 21 621.6 0 21 28 23 621.6 0 23 29 19 621.6 0 19 30 23 621.6 0 23 31 22 621.6 0 22 32 19 621.6 0 19 33 26 621.6 0 26 34 24 621.6 0 24 35 22 621.6 0 22 36 28 621.6 0 28 37 16 621.6 0 16 38 16 621.6 0 16 39 20 621.6 0 20 40 35 621.6 0 35 41 22 621.6 0 22 42 22 621.6 0 22 43 21 621.6 0 21 44 22 621.6 0 22 45 14 621.6 0 14 46 23 621.6 0 23 47 23 621.6 0 23 48 21 621.6 0 21 49 10 621.6 0 10 50 22 621.6 0 22 51 10 621.6 0 10 52 14 621.6 0 14 53 20 621.6 0 20 54 10 621.6 0 10 55 34 621.6 0 34 56 32 621.6 0 32 57 34 621.6 0 34 58 29 621.6 0 29 59 25 621.6 0 25 60 21 621.6 0 21 61 21 621.6 0 21 62 16 621.6 0 16 63 27 621.6 0 27 64 16 621.6 0 16 65 29 621.6 0 29 66 30 621.6 0 30 67 19 621.6 0 19 68 26 621.6 0 26 69 15 621.6 0 15 70 11 621.6 0 11 71 21 621.6 0 21 72 17 621.6 0 17 73 28 621.6 0 28 74 26 621.6 0 26 75 54 621.6 0 54 76 24 621.6 0 24 77 28 621.6 0 28 78 16 621.6 0 16 79 26 621.6 0 26 80 18 621.6 0 18 81 24 621.6 0 24 82 17 621.6 0 17 83 22 621.6 0 22 84 36 621.6 0 36 85 18 621.6 0 18 86 18 621.6 0 18 87 33 621.6 0 33 88 24 621.6 0 24 89 33 621.6 0 33 90 33 621.6 0 33 91 22 621.6 0 22 92 23 621.6 0 23 93 18 621.6 0 18 94 30 621.6 0 30 95 28 621.6 0 28 96 19 621.6 0 19 97 20 621.6 0 20 98 22 621.6 0 22 99 20 621.6 0 20 100 12 621.6 0 12 101 28 621.6 0 28 102 18 621.6 0 18 103 62 621.6 0 62 104 50 621.6 0 50 105 115 621.6 0 115 106 133 621.6 0 133 107 128 621.6 0 128 108 14 621.6 0 14 109 39 621.6 0 39 110 44 621.6 0 44 111 44 621.6 0 44 112 39 621.6 0 39 113 46 621.6 0 46 114 47 621.6 0 47 115 44 621.6 0 44 116 49 621.6 0 49 117 37 621.6 0 37 118 46 621.6 0 46 119 21 621.6 0 21 120 29 621.6 0 29 121 40 621.6 0 40 122 36 621.6 0 36 123 47 621.6 0 47 124 36 621.6 0 36 125 48 621.6 0 48 126 106 621.6 0 106 127 237 621.6 0 237 128 858 621.6 0 858 129 355 621.6 0 355 130 596 621.6 0 596 131 67 621.6 0 67 132 79 621.6 0 79 133 106 621.6 0 106 134 154 621.6 0 154 135 60 621.6 0 60 136 77 621.6 0 77 137 198 621.6 0 198 138 63 621.6 0 63 139 59 621.6 0 59 140 104 621.6 0 104 141 88 621.6 0 88 142 165 621.6 0 165 143 103 621.6 0 103 144 174 621.6 0 174 145 182 621.6 0 182 146 201 621.6 0 201 147 4176 621.6 0 4176 148 3643 621.6 0 3643 149 8465 621.6 0 8465 150 116 621.6 0 116 151 32 621.6 0 32 152 96 621.6 0 96 153 41 621.6 0 41 154 109 621.6 0 109 155 48 621.6 0 48 156 43 621.6 0 43 157 40 621.6 0 40 158 35 621.6 0 35 159 39 621.6 0 39 160 34 621.6 0 34 161 37 621.6 0 37 162 36 621.6 0 36 163 24 621.6 0 24 164 42 621.6 0 42 165 27 621.6 0 27 166 27 621.6 0 27 167 24 621.6 0 24 168 54 621.6 0 54 169 105 621.6 0 105 170 70 621.6 0 70 171 83 621.6 0 83 172 168 621.6 0 168 173 108 621.6 0 108 174 151 621.6 0 151 175 37 621.6 0 37 176 31 621.6 0 31 177 55 621.6 0 55 178 40 621.6 0 40 179 35 621.6 0 35 180 37 621.6 0 37 181 36 621.6 0 36 182 29 621.6 0 29 183 74 621.6 0 74 184 96 621.6 0 96 185 34 621.6 0 34 186 58 621.6 0 58 187 179 621.6 0 179 188 1291 621.6 0 1291 189 986 621.6 0 986 190 1786 621.6 0 1786 191 50 621.6 0 50 192 25 621.6 0 25 193 37 621.6 0 37 194 36 621.6 0 36 195 26 621.6 0 26 196 71 621.6 0 71 197 104 621.6 0 104 198 89 621.6 0 89 199 161 621.6 0 161 200 496 621.6 0 496 201 3144 621.6 0 3144 202 1927 621.6 0 1927 203 3533 621.6 0 3533 204 182 621.6 0 182 205 26 621.6 0 26 206 22 621.6 0 22 207 26 621.6 0 26 208 36 621.6 0 36 209 13 621.6 0 13 210 27 621.6 0 27 211 25 621.6 0 25 212 24 621.6 0 24 213 20 621.6 0 20 214 14 621.6 0 14 215 28 621.6 0 28 216 25 621.6 0 25 217 24 621.6 0 24 218 17 621.6 0 17 219 15 621.6 0 15 220 24 621.6 0 24 221 18 621.6 0 18 222 25 621.6 0 25 223 19 621.6 0 19 224 16 621.6 0 16 225 44 621.6 0 44 226 61 621.6 0 61 227 39 621.6 0 39 228 88 621.6 0 88 229 304 621.6 0 304 230 72 621.6 0 72 231 24 621.6 0 24 232 29 621.6 0 29 233 18 621.6 0 18 234 23 621.6 0 23 235 22 621.6 0 22 236 45 621.6 0 45 237 29 621.6 0 29 238 34 621.6 0 34 239 22 621.6 0 22 240 29 621.6 0 29 241 28 621.6 0 28 242 31 621.6 0 31 243 38 621.6 0 38 244 31 621.6 0 31 245 26 621.6 0 26 246 21 621.6 0 21 247 17 621.6 0 17 248 18 621.6 0 18 249 47 621.6 0 47 250 47 621.6 0 47 251 50 621.6 0 50 252 63 621.6 0 63 253 21 621.6 0 21 254 26 621.6 0 26 255 17 621.6 0 17 256 18 621.6 0 18 257 16 621.6 0 16 258 41 621.6 0 41 259 39 621.6 0 39 260 59 621.6 0 59 261 62 621.6 0 62 262 40 621.6 0 40 263 38 621.6 0 38 264 18 621.6 0 18 265 31 621.6 0 31 266 28 621.6 0 28 267 37 621.6 0 37 268 41 621.6 0 41 269 220 621.6 0 220 270 85 621.6 0 85 271 124 621.6 0 124 272 51 621.6 0 51 273 35 621.6 0 35 274 26 621.6 0 26 275 56 621.6 0 56 276 71 621.6 0 71 277 44 621.6 0 44 278 45 621.6 0 45 279 64 621.6 0 64 280 104 621.6 0 104 281 45 621.6 0 45 282 162 621.6 0 162 283 75 621.6 0 75 284 101 621.6 0 101 285 23 621.6 0 23 286 32 621.6 0 32 287 61 621.6 0 61 288 37 621.6 0 37 289 37 621.6 0 37 290 32 621.6 0 32 291 145 621.6 0 145 292 91 621.6 0 91 293 166 621.6 0 166 294 183 621.6 0 183 295 131 621.6 0 131 296 162 621.6 0 162 297 66 621.6 0 66 298 18 621.6 0 18 299 55 621.6 0 55 300 51 621.6 0 51 301 38 621.6 0 38 Finished in 2.05 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 47,985 Reads with adapters: 7,371 (15.4%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 7,363 (15.3%) Total basepairs processed: 9,119,936 bp Total written (filtered): 2,002,555 bp (22.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 7371 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 3 0.0 3 0 0 3 19 88 0.0 3 0 86 2 20 5638 0.0 4 5542 91 5 21 29 0.0 4 9 20 22 1591 0.0 4 1566 23 2 23 4 0.0 4 1 0 0 3 25 3 0.0 4 0 0 0 2 1 53 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 88 1 0.0 4 1 127 1 0.0 4 0 0 0 0 1 129 5 0.0 4 0 0 0 0 5 136 1 0.0 4 0 0 0 0 1 144 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 1 228 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.26 s (35 us/read; 1.70 M reads/minute). === Summary === Total reads processed: 7,363 Reads with adapters: 882 (12.0%) Reads written (passing filters): 7,363 (100.0%) Total basepairs processed: 2,002,555 bp Total written (filtered): 1,985,347 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 882 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 10.8% C: 2.2% G: 85.7% T: 1.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 115.0 0 1 4 1 28.8 0 1 9 167 0.0 0 6 161 10 558 0.0 1 14 544 11 69 0.0 1 0 69 13 1 0.0 1 0 1 97 1 0.0 1 0 1 110 76 0.0 1 65 11 111 7 0.0 1 5 2 112 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.