This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACACAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.16 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 69,700 (2.7%) Reads that were too short: 33,781 (1.3%) Reads written (passing filters): 35,919 (1.4%) Total basepairs processed: 766,381,518 bp Total written (filtered): 7,494,072 bp (1.0%) === Adapter 1 === Sequence: ACACAA; Type: regular 5'; Length: 6; Trimmed: 69700 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 642 621.6 0 642 7 5861 621.6 0 5861 8 99 621.6 0 99 9 4986 621.6 0 4986 10 32 621.6 0 32 11 19 621.6 0 19 12 28 621.6 0 28 13 17 621.6 0 17 14 20 621.6 0 20 15 29 621.6 0 29 16 32 621.6 0 32 17 28 621.6 0 28 18 17 621.6 0 17 19 30 621.6 0 30 20 21 621.6 0 21 21 22 621.6 0 22 22 36 621.6 0 36 23 25 621.6 0 25 24 9 621.6 0 9 25 31 621.6 0 31 26 31 621.6 0 31 27 15 621.6 0 15 28 43 621.6 0 43 29 27 621.6 0 27 30 37 621.6 0 37 31 20 621.6 0 20 32 35 621.6 0 35 33 22 621.6 0 22 34 22 621.6 0 22 35 14 621.6 0 14 36 24 621.6 0 24 37 15 621.6 0 15 38 27 621.6 0 27 39 21 621.6 0 21 40 40 621.6 0 40 41 128 621.6 0 128 42 92 621.6 0 92 43 169 621.6 0 169 44 23 621.6 0 23 45 22 621.6 0 22 46 34 621.6 0 34 47 31 621.6 0 31 48 35 621.6 0 35 49 28 621.6 0 28 50 31 621.6 0 31 51 40 621.6 0 40 52 40 621.6 0 40 53 24 621.6 0 24 54 16 621.6 0 16 55 20 621.6 0 20 56 30 621.6 0 30 57 28 621.6 0 28 58 30 621.6 0 30 59 31 621.6 0 31 60 29 621.6 0 29 61 25 621.6 0 25 62 35 621.6 0 35 63 36 621.6 0 36 64 26 621.6 0 26 65 20 621.6 0 20 66 21 621.6 0 21 67 29 621.6 0 29 68 19 621.6 0 19 69 53 621.6 0 53 70 51 621.6 0 51 71 34 621.6 0 34 72 46 621.6 0 46 73 44 621.6 0 44 74 78 621.6 0 78 75 38 621.6 0 38 76 58 621.6 0 58 77 77 621.6 0 77 78 66 621.6 0 66 79 139 621.6 0 139 80 166 621.6 0 166 81 151 621.6 0 151 82 165 621.6 0 165 83 185 621.6 0 185 84 179 621.6 0 179 85 138 621.6 0 138 86 97 621.6 0 97 87 76 621.6 0 76 88 84 621.6 0 84 89 76 621.6 0 76 90 76 621.6 0 76 91 106 621.6 0 106 92 88 621.6 0 88 93 97 621.6 0 97 94 59 621.6 0 59 95 90 621.6 0 90 96 87 621.6 0 87 97 77 621.6 0 77 98 74 621.6 0 74 99 73 621.6 0 73 100 77 621.6 0 77 101 111 621.6 0 111 102 118 621.6 0 118 103 114 621.6 0 114 104 144 621.6 0 144 105 158 621.6 0 158 106 194 621.6 0 194 107 172 621.6 0 172 108 144 621.6 0 144 109 149 621.6 0 149 110 170 621.6 0 170 111 159 621.6 0 159 112 160 621.6 0 160 113 182 621.6 0 182 114 158 621.6 0 158 115 156 621.6 0 156 116 251 621.6 0 251 117 164 621.6 0 164 118 178 621.6 0 178 119 190 621.6 0 190 120 198 621.6 0 198 121 148 621.6 0 148 122 139 621.6 0 139 123 157 621.6 0 157 124 174 621.6 0 174 125 198 621.6 0 198 126 318 621.6 0 318 127 152 621.6 0 152 128 134 621.6 0 134 129 139 621.6 0 139 130 164 621.6 0 164 131 188 621.6 0 188 132 237 621.6 0 237 133 1327 621.6 0 1327 134 750 621.6 0 750 135 1168 621.6 0 1168 136 142 621.6 0 142 137 130 621.6 0 130 138 139 621.6 0 139 139 138 621.6 0 138 140 129 621.6 0 129 141 127 621.6 0 127 142 144 621.6 0 144 143 130 621.6 0 130 144 161 621.6 0 161 145 149 621.6 0 149 146 159 621.6 0 159 147 329 621.6 0 329 148 159 621.6 0 159 149 139 621.6 0 139 150 138 621.6 0 138 151 121 621.6 0 121 152 125 621.6 0 125 153 138 621.6 0 138 154 128 621.6 0 128 155 148 621.6 0 148 156 140 621.6 0 140 157 148 621.6 0 148 158 217 621.6 0 217 159 236 621.6 0 236 160 207 621.6 0 207 161 183 621.6 0 183 162 351 621.6 0 351 163 265 621.6 0 265 164 357 621.6 0 357 165 164 621.6 0 164 166 132 621.6 0 132 167 132 621.6 0 132 168 132 621.6 0 132 169 141 621.6 0 141 170 144 621.6 0 144 171 211 621.6 0 211 172 187 621.6 0 187 173 168 621.6 0 168 174 134 621.6 0 134 175 147 621.6 0 147 176 111 621.6 0 111 177 117 621.6 0 117 178 156 621.6 0 156 179 191 621.6 0 191 180 169 621.6 0 169 181 153 621.6 0 153 182 168 621.6 0 168 183 153 621.6 0 153 184 119 621.6 0 119 185 197 621.6 0 197 186 168 621.6 0 168 187 144 621.6 0 144 188 151 621.6 0 151 189 318 621.6 0 318 190 157 621.6 0 157 191 181 621.6 0 181 192 182 621.6 0 182 193 178 621.6 0 178 194 114 621.6 0 114 195 116 621.6 0 116 196 148 621.6 0 148 197 128 621.6 0 128 198 138 621.6 0 138 199 147 621.6 0 147 200 142 621.6 0 142 201 167 621.6 0 167 202 148 621.6 0 148 203 158 621.6 0 158 204 135 621.6 0 135 205 137 621.6 0 137 206 132 621.6 0 132 207 165 621.6 0 165 208 177 621.6 0 177 209 202 621.6 0 202 210 222 621.6 0 222 211 364 621.6 0 364 212 744 621.6 0 744 213 6614 621.6 0 6614 214 2717 621.6 0 2717 215 3288 621.6 0 3288 216 302 621.6 0 302 217 213 621.6 0 213 218 174 621.6 0 174 219 187 621.6 0 187 220 139 621.6 0 139 221 160 621.6 0 160 222 185 621.6 0 185 223 219 621.6 0 219 224 250 621.6 0 250 225 511 621.6 0 511 226 597 621.6 0 597 227 788 621.6 0 788 228 252 621.6 0 252 229 211 621.6 0 211 230 176 621.6 0 176 231 195 621.6 0 195 232 150 621.6 0 150 233 161 621.6 0 161 234 153 621.6 0 153 235 164 621.6 0 164 236 155 621.6 0 155 237 168 621.6 0 168 238 166 621.6 0 166 239 141 621.6 0 141 240 134 621.6 0 134 241 153 621.6 0 153 242 155 621.6 0 155 243 170 621.6 0 170 244 154 621.6 0 154 245 137 621.6 0 137 246 150 621.6 0 150 247 185 621.6 0 185 248 195 621.6 0 195 249 211 621.6 0 211 250 401 621.6 0 401 251 251 621.6 0 251 252 339 621.6 0 339 253 233 621.6 0 233 254 220 621.6 0 220 255 182 621.6 0 182 256 181 621.6 0 181 257 172 621.6 0 172 258 150 621.6 0 150 259 165 621.6 0 165 260 189 621.6 0 189 261 178 621.6 0 178 262 180 621.6 0 180 263 184 621.6 0 184 264 220 621.6 0 220 265 216 621.6 0 216 266 229 621.6 0 229 267 158 621.6 0 158 268 224 621.6 0 224 269 181 621.6 0 181 270 246 621.6 0 246 271 231 621.6 0 231 272 214 621.6 0 214 273 202 621.6 0 202 274 198 621.6 0 198 275 189 621.6 0 189 276 287 621.6 0 287 277 255 621.6 0 255 278 261 621.6 0 261 279 232 621.6 0 232 280 199 621.6 0 199 281 223 621.6 0 223 282 245 621.6 0 245 283 232 621.6 0 232 284 236 621.6 0 236 285 213 621.6 0 213 286 189 621.6 0 189 287 194 621.6 0 194 288 170 621.6 0 170 289 232 621.6 0 232 290 288 621.6 0 288 291 251 621.6 0 251 292 204 621.6 0 204 293 160 621.6 0 160 294 181 621.6 0 181 295 189 621.6 0 189 296 161 621.6 0 161 297 181 621.6 0 181 298 138 621.6 0 138 299 159 621.6 0 159 300 198 621.6 0 198 301 306 621.6 0 306 Finished in 1.60 s (45 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 35,919 Reads with adapters: 6,450 (18.0%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 6,438 (17.9%) Total basepairs processed: 7,494,072 bp Total written (filtered): 1,758,318 bp (23.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6450 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 3 0.0 3 0 0 3 19 128 0.0 3 0 124 4 20 5702 0.0 4 5612 85 4 0 1 21 30 0.0 4 1 28 0 1 22 525 0.0 4 516 7 0 0 2 23 3 0.0 4 0 0 0 2 1 24 1 0.0 4 1 38 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 127 45 0.0 4 0 0 0 0 45 137 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 259 1 0.0 4 0 0 0 0 1 264 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.22 s (34 us/read; 1.76 M reads/minute). === Summary === Total reads processed: 6,438 Reads with adapters: 151 (2.3%) Reads written (passing filters): 6,438 (100.0%) Total basepairs processed: 1,758,318 bp Total written (filtered): 1,756,153 bp (99.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 151 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 9.9% C: 6.6% G: 82.8% T: 0.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 19 100.6 0 19 4 1 25.1 0 1 5 1 6.3 0 1 9 1 0.0 0 1 10 12 0.0 1 0 12 11 108 0.0 1 1 107 12 4 0.0 1 0 4 13 2 0.0 1 0 2 210 1 0.0 1 0 1 225 1 0.0 1 1 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.