This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.13 s (26 us/read; 2.35 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 347,083 (13.6%) Reads that were too short: 292,855 (11.5%) Reads written (passing filters): 54,228 (2.1%) Total basepairs processed: 766,381,518 bp Total written (filtered): 12,154,928 bp (1.6%) === Adapter 1 === Sequence: GCGGTT; Type: regular 5'; Length: 6; Trimmed: 347083 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 306 621.6 0 306 7 633 621.6 0 633 8 15404 621.6 0 15404 9 11805 621.6 0 11805 10 76 621.6 0 76 11 50 621.6 0 50 12 48 621.6 0 48 13 46 621.6 0 46 14 56 621.6 0 56 15 36 621.6 0 36 16 50 621.6 0 50 17 54 621.6 0 54 18 39 621.6 0 39 19 39 621.6 0 39 20 122 621.6 0 122 21 94 621.6 0 94 22 139 621.6 0 139 23 59 621.6 0 59 24 63 621.6 0 63 25 54 621.6 0 54 26 47 621.6 0 47 27 55 621.6 0 55 28 42 621.6 0 42 29 41 621.6 0 41 30 21 621.6 0 21 31 43 621.6 0 43 32 49 621.6 0 49 33 150 621.6 0 150 34 58 621.6 0 58 35 80 621.6 0 80 36 49 621.6 0 49 37 34 621.6 0 34 38 48 621.6 0 48 39 51 621.6 0 51 40 43 621.6 0 43 41 40 621.6 0 40 42 50 621.6 0 50 43 42 621.6 0 42 44 48 621.6 0 48 45 48 621.6 0 48 46 48 621.6 0 48 47 63 621.6 0 63 48 45 621.6 0 45 49 53 621.6 0 53 50 51 621.6 0 51 51 57 621.6 0 57 52 30 621.6 0 30 53 44 621.6 0 44 54 40 621.6 0 40 55 36 621.6 0 36 56 45 621.6 0 45 57 49 621.6 0 49 58 42 621.6 0 42 59 24 621.6 0 24 60 30 621.6 0 30 61 48 621.6 0 48 62 45 621.6 0 45 63 84 621.6 0 84 64 87 621.6 0 87 65 59 621.6 0 59 66 102 621.6 0 102 67 113 621.6 0 113 68 51 621.6 0 51 69 54 621.6 0 54 70 32 621.6 0 32 71 57 621.6 0 57 72 29 621.6 0 29 73 62 621.6 0 62 74 68 621.6 0 68 75 76 621.6 0 76 76 52 621.6 0 52 77 59 621.6 0 59 78 54 621.6 0 54 79 52 621.6 0 52 80 50 621.6 0 50 81 39 621.6 0 39 82 57 621.6 0 57 83 25 621.6 0 25 84 46 621.6 0 46 85 47 621.6 0 47 86 68 621.6 0 68 87 58 621.6 0 58 88 89 621.6 0 89 89 73 621.6 0 73 90 58 621.6 0 58 91 66 621.6 0 66 92 68 621.6 0 68 93 94 621.6 0 94 94 122 621.6 0 122 95 162 621.6 0 162 96 73 621.6 0 73 97 93 621.6 0 93 98 60 621.6 0 60 99 127 621.6 0 127 100 62 621.6 0 62 101 52 621.6 0 52 102 44 621.6 0 44 103 44 621.6 0 44 104 40 621.6 0 40 105 37 621.6 0 37 106 52 621.6 0 52 107 45 621.6 0 45 108 54 621.6 0 54 109 51 621.6 0 51 110 40 621.6 0 40 111 36 621.6 0 36 112 43 621.6 0 43 113 44 621.6 0 44 114 33 621.6 0 33 115 33 621.6 0 33 116 55 621.6 0 55 117 66 621.6 0 66 118 56 621.6 0 56 119 71 621.6 0 71 120 67 621.6 0 67 121 48 621.6 0 48 122 53 621.6 0 53 123 54 621.6 0 54 124 115 621.6 0 115 125 110 621.6 0 110 126 136 621.6 0 136 127 241 621.6 0 241 128 75 621.6 0 75 129 58 621.6 0 58 130 228 621.6 0 228 131 461 621.6 0 461 132 685 621.6 0 685 133 438 621.6 0 438 134 53 621.6 0 53 135 47 621.6 0 47 136 51 621.6 0 51 137 42 621.6 0 42 138 54 621.6 0 54 139 65 621.6 0 65 140 51 621.6 0 51 141 77 621.6 0 77 142 48 621.6 0 48 143 47 621.6 0 47 144 31 621.6 0 31 145 78 621.6 0 78 146 54 621.6 0 54 147 76 621.6 0 76 148 75 621.6 0 75 149 43 621.6 0 43 150 44 621.6 0 44 151 57 621.6 0 57 152 57 621.6 0 57 153 85 621.6 0 85 154 108 621.6 0 108 155 104 621.6 0 104 156 84 621.6 0 84 157 56 621.6 0 56 158 58 621.6 0 58 159 50 621.6 0 50 160 47 621.6 0 47 161 44 621.6 0 44 162 39 621.6 0 39 163 45 621.6 0 45 164 57 621.6 0 57 165 47 621.6 0 47 166 70 621.6 0 70 167 85 621.6 0 85 168 64 621.6 0 64 169 69 621.6 0 69 170 53 621.6 0 53 171 63 621.6 0 63 172 63 621.6 0 63 173 133 621.6 0 133 174 251 621.6 0 251 175 278 621.6 0 278 176 224 621.6 0 224 177 84 621.6 0 84 178 91 621.6 0 91 179 442 621.6 0 442 180 312 621.6 0 312 181 501 621.6 0 501 182 159 621.6 0 159 183 101 621.6 0 101 184 95 621.6 0 95 185 230 621.6 0 230 186 301 621.6 0 301 187 477 621.6 0 477 188 199 621.6 0 199 189 127 621.6 0 127 190 129 621.6 0 129 191 214 621.6 0 214 192 477 621.6 0 477 193 2423 621.6 0 2423 194 1842 621.6 0 1842 195 2505 621.6 0 2505 196 165 621.6 0 165 197 142 621.6 0 142 198 145 621.6 0 145 199 868 621.6 0 868 200 801 621.6 0 801 201 916 621.6 0 916 202 368 621.6 0 368 203 99 621.6 0 99 204 86 621.6 0 86 205 128 621.6 0 128 206 221 621.6 0 221 207 229 621.6 0 229 208 153 621.6 0 153 209 86 621.6 0 86 210 197 621.6 0 197 211 249 621.6 0 249 212 172 621.6 0 172 213 338 621.6 0 338 214 143 621.6 0 143 215 169 621.6 0 169 216 116 621.6 0 116 217 120 621.6 0 120 218 146 621.6 0 146 219 279 621.6 0 279 220 322 621.6 0 322 221 401 621.6 0 401 222 453 621.6 0 453 223 671 621.6 0 671 224 951 621.6 0 951 225 286 621.6 0 286 226 345 621.6 0 345 227 611 621.6 0 611 228 360 621.6 0 360 229 372 621.6 0 372 230 695 621.6 0 695 231 1001 621.6 0 1001 232 2977 621.6 0 2977 233 3358 621.6 0 3358 234 5537 621.6 0 5537 235 1937 621.6 0 1937 236 2435 621.6 0 2435 237 43406 621.6 0 43406 238 26484 621.6 0 26484 239 48677 621.6 0 48677 240 1687 621.6 0 1687 241 750 621.6 0 750 242 675 621.6 0 675 243 618 621.6 0 618 244 734 621.6 0 734 245 378 621.6 0 378 246 302 621.6 0 302 247 559 621.6 0 559 248 445 621.6 0 445 249 522 621.6 0 522 250 714 621.6 0 714 251 667 621.6 0 667 252 1272 621.6 0 1272 253 1052 621.6 0 1052 254 1654 621.6 0 1654 255 1904 621.6 0 1904 256 2250 621.6 0 2250 257 35144 621.6 0 35144 258 34875 621.6 0 34875 259 41417 621.6 0 41417 260 14866 621.6 0 14866 261 189 621.6 0 189 262 281 621.6 0 281 263 97 621.6 0 97 264 52 621.6 0 52 265 42 621.6 0 42 266 50 621.6 0 50 267 82 621.6 0 82 268 127 621.6 0 127 269 94 621.6 0 94 270 112 621.6 0 112 271 63 621.6 0 63 272 73 621.6 0 73 273 67 621.6 0 67 274 68 621.6 0 68 275 60 621.6 0 60 276 71 621.6 0 71 277 61 621.6 0 61 278 49 621.6 0 49 279 83 621.6 0 83 280 73 621.6 0 73 281 85 621.6 0 85 282 111 621.6 0 111 283 116 621.6 0 116 284 125 621.6 0 125 285 204 621.6 0 204 286 177 621.6 0 177 287 307 621.6 0 307 288 164 621.6 0 164 289 180 621.6 0 180 290 218 621.6 0 218 291 386 621.6 0 386 292 316 621.6 0 316 293 524 621.6 0 524 294 234 621.6 0 234 295 137 621.6 0 137 296 142 621.6 0 142 297 116 621.6 0 116 298 63 621.6 0 63 299 82 621.6 0 82 300 118 621.6 0 118 301 193 621.6 0 193 Finished in 2.33 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 54,228 Reads with adapters: 15,205 (28.0%) Reads that were too short: 111 (0.2%) Reads written (passing filters): 15,094 (27.8%) Total basepairs processed: 12,154,928 bp Total written (filtered): 4,155,126 bp (34.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 15205 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 11 0.0 3 0 0 10 1 16 139 0.0 3 0 109 19 11 17 14729 0.0 3 13677 848 166 38 18 68 0.0 3 1 42 3 22 19 47 0.0 3 1 0 29 17 20 38 0.0 3 0 0 0 38 24 2 0.0 3 0 0 0 2 30 1 0.0 3 1 31 2 0.0 3 0 0 0 2 34 2 0.0 3 0 0 0 2 35 1 0.0 3 1 36 3 0.0 3 0 0 0 3 37 3 0.0 3 0 0 0 3 38 2 0.0 3 0 0 0 2 43 8 0.0 3 0 0 0 8 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 47 2 0.0 3 0 0 0 2 48 2 0.0 3 0 0 0 2 49 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 60 15 0.0 3 0 0 0 15 61 1 0.0 3 0 0 0 1 62 12 0.0 3 0 0 0 12 63 2 0.0 3 0 0 0 2 64 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 73 1 0.0 3 0 0 0 1 74 4 0.0 3 0 0 1 3 82 1 0.0 3 0 0 0 1 84 7 0.0 3 0 0 2 5 86 1 0.0 3 0 0 0 1 88 4 0.0 3 0 0 0 4 89 2 0.0 3 0 0 0 2 92 1 0.0 3 0 0 0 1 93 9 0.0 3 0 0 0 9 94 4 0.0 3 0 0 0 4 95 1 0.0 3 0 0 0 1 97 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 5 0.0 3 0 0 0 5 106 1 0.0 3 0 0 0 1 107 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 2 0.0 3 0 0 0 2 113 1 0.0 3 0 0 0 1 117 1 0.0 3 0 0 0 1 122 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 144 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 7 0.0 3 0 0 0 7 163 3 0.0 3 0 0 0 3 164 2 0.0 3 0 0 1 1 167 1 0.0 3 0 0 1 169 2 0.0 3 0 0 1 1 188 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 195 4 0.0 3 0 0 0 4 198 2 0.0 3 0 0 0 2 205 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 239 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 256 1 0.0 3 0 0 0 1 274 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.53 s (35 us/read; 1.71 M reads/minute). === Summary === Total reads processed: 15,094 Reads with adapters: 25 (0.2%) Reads written (passing filters): 15,094 (100.0%) Total basepairs processed: 4,155,126 bp Total written (filtered): 4,155,039 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 25 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 44.0% C: 8.0% G: 24.0% T: 24.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 17 235.8 0 17 4 4 59.0 0 4 5 4 14.7 0 4