This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGGTT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 66.71 s (26 us/read; 2.29 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 376,126 (14.8%) Reads that were too short: 319,354 (12.5%) Reads written (passing filters): 56,772 (2.2%) Total basepairs processed: 766,381,518 bp Total written (filtered): 13,041,318 bp (1.7%) === Adapter 1 === Sequence: GCGGTT; Type: regular 5'; Length: 6; Trimmed: 376126 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 330 621.6 0 330 7 502 621.6 0 502 8 14331 621.6 0 14331 9 14815 621.6 0 14815 10 99 621.6 0 99 11 87 621.6 0 87 12 75 621.6 0 75 13 45 621.6 0 45 14 83 621.6 0 83 15 49 621.6 0 49 16 74 621.6 0 74 17 95 621.6 0 95 18 87 621.6 0 87 19 64 621.6 0 64 20 78 621.6 0 78 21 66 621.6 0 66 22 58 621.6 0 58 23 75 621.6 0 75 24 55 621.6 0 55 25 53 621.6 0 53 26 66 621.6 0 66 27 69 621.6 0 69 28 61 621.6 0 61 29 60 621.6 0 60 30 49 621.6 0 49 31 58 621.6 0 58 32 39 621.6 0 39 33 56 621.6 0 56 34 61 621.6 0 61 35 65 621.6 0 65 36 79 621.6 0 79 37 77 621.6 0 77 38 59 621.6 0 59 39 85 621.6 0 85 40 56 621.6 0 56 41 62 621.6 0 62 42 60 621.6 0 60 43 60 621.6 0 60 44 58 621.6 0 58 45 59 621.6 0 59 46 81 621.6 0 81 47 73 621.6 0 73 48 84 621.6 0 84 49 68 621.6 0 68 50 62 621.6 0 62 51 69 621.6 0 69 52 49 621.6 0 49 53 57 621.6 0 57 54 48 621.6 0 48 55 63 621.6 0 63 56 61 621.6 0 61 57 89 621.6 0 89 58 65 621.6 0 65 59 33 621.6 0 33 60 66 621.6 0 66 61 64 621.6 0 64 62 49 621.6 0 49 63 104 621.6 0 104 64 112 621.6 0 112 65 65 621.6 0 65 66 117 621.6 0 117 67 110 621.6 0 110 68 66 621.6 0 66 69 78 621.6 0 78 70 68 621.6 0 68 71 96 621.6 0 96 72 59 621.6 0 59 73 67 621.6 0 67 74 76 621.6 0 76 75 87 621.6 0 87 76 60 621.6 0 60 77 77 621.6 0 77 78 79 621.6 0 79 79 81 621.6 0 81 80 49 621.6 0 49 81 66 621.6 0 66 82 98 621.6 0 98 83 63 621.6 0 63 84 65 621.6 0 65 85 40 621.6 0 40 86 87 621.6 0 87 87 71 621.6 0 71 88 96 621.6 0 96 89 96 621.6 0 96 90 84 621.6 0 84 91 90 621.6 0 90 92 99 621.6 0 99 93 118 621.6 0 118 94 67 621.6 0 67 95 68 621.6 0 68 96 56 621.6 0 56 97 75 621.6 0 75 98 69 621.6 0 69 99 88 621.6 0 88 100 71 621.6 0 71 101 59 621.6 0 59 102 93 621.6 0 93 103 81 621.6 0 81 104 63 621.6 0 63 105 76 621.6 0 76 106 90 621.6 0 90 107 70 621.6 0 70 108 83 621.6 0 83 109 82 621.6 0 82 110 70 621.6 0 70 111 77 621.6 0 77 112 64 621.6 0 64 113 80 621.6 0 80 114 70 621.6 0 70 115 51 621.6 0 51 116 98 621.6 0 98 117 96 621.6 0 96 118 74 621.6 0 74 119 93 621.6 0 93 120 87 621.6 0 87 121 77 621.6 0 77 122 77 621.6 0 77 123 105 621.6 0 105 124 103 621.6 0 103 125 109 621.6 0 109 126 151 621.6 0 151 127 124 621.6 0 124 128 96 621.6 0 96 129 89 621.6 0 89 130 206 621.6 0 206 131 363 621.6 0 363 132 408 621.6 0 408 133 460 621.6 0 460 134 98 621.6 0 98 135 79 621.6 0 79 136 82 621.6 0 82 137 107 621.6 0 107 138 94 621.6 0 94 139 75 621.6 0 75 140 106 621.6 0 106 141 122 621.6 0 122 142 89 621.6 0 89 143 96 621.6 0 96 144 109 621.6 0 109 145 103 621.6 0 103 146 116 621.6 0 116 147 125 621.6 0 125 148 131 621.6 0 131 149 112 621.6 0 112 150 109 621.6 0 109 151 111 621.6 0 111 152 137 621.6 0 137 153 137 621.6 0 137 154 162 621.6 0 162 155 150 621.6 0 150 156 121 621.6 0 121 157 125 621.6 0 125 158 139 621.6 0 139 159 110 621.6 0 110 160 123 621.6 0 123 161 125 621.6 0 125 162 113 621.6 0 113 163 129 621.6 0 129 164 130 621.6 0 130 165 156 621.6 0 156 166 134 621.6 0 134 167 157 621.6 0 157 168 169 621.6 0 169 169 145 621.6 0 145 170 148 621.6 0 148 171 146 621.6 0 146 172 140 621.6 0 140 173 205 621.6 0 205 174 273 621.6 0 273 175 296 621.6 0 296 176 211 621.6 0 211 177 167 621.6 0 167 178 141 621.6 0 141 179 207 621.6 0 207 180 202 621.6 0 202 181 324 621.6 0 324 182 190 621.6 0 190 183 152 621.6 0 152 184 172 621.6 0 172 185 297 621.6 0 297 186 342 621.6 0 342 187 440 621.6 0 440 188 281 621.6 0 281 189 216 621.6 0 216 190 222 621.6 0 222 191 270 621.6 0 270 192 346 621.6 0 346 193 1510 621.6 0 1510 194 866 621.6 0 866 195 2356 621.6 0 2356 196 188 621.6 0 188 197 190 621.6 0 190 198 187 621.6 0 187 199 455 621.6 0 455 200 481 621.6 0 481 201 441 621.6 0 441 202 247 621.6 0 247 203 172 621.6 0 172 204 166 621.6 0 166 205 212 621.6 0 212 206 212 621.6 0 212 207 231 621.6 0 231 208 185 621.6 0 185 209 152 621.6 0 152 210 248 621.6 0 248 211 297 621.6 0 297 212 234 621.6 0 234 213 399 621.6 0 399 214 271 621.6 0 271 215 239 621.6 0 239 216 255 621.6 0 255 217 239 621.6 0 239 218 245 621.6 0 245 219 375 621.6 0 375 220 345 621.6 0 345 221 386 621.6 0 386 222 473 621.6 0 473 223 735 621.6 0 735 224 1038 621.6 0 1038 225 403 621.6 0 403 226 458 621.6 0 458 227 714 621.6 0 714 228 432 621.6 0 432 229 460 621.6 0 460 230 802 621.6 0 802 231 1074 621.6 0 1074 232 3530 621.6 0 3530 233 4111 621.6 0 4111 234 6680 621.6 0 6680 235 1922 621.6 0 1922 236 1928 621.6 0 1928 237 43403 621.6 0 43403 238 27241 621.6 0 27241 239 49743 621.6 0 49743 240 1598 621.6 0 1598 241 806 621.6 0 806 242 755 621.6 0 755 243 699 621.6 0 699 244 971 621.6 0 971 245 516 621.6 0 516 246 436 621.6 0 436 247 679 621.6 0 679 248 541 621.6 0 541 249 614 621.6 0 614 250 787 621.6 0 787 251 858 621.6 0 858 252 1396 621.6 0 1396 253 1238 621.6 0 1238 254 1828 621.6 0 1828 255 2139 621.6 0 2139 256 1946 621.6 0 1946 257 38840 621.6 0 38840 258 37885 621.6 0 37885 259 44141 621.6 0 44141 260 21413 621.6 0 21413 261 244 621.6 0 244 262 194 621.6 0 194 263 178 621.6 0 178 264 163 621.6 0 163 265 170 621.6 0 170 266 152 621.6 0 152 267 170 621.6 0 170 268 215 621.6 0 215 269 160 621.6 0 160 270 191 621.6 0 191 271 157 621.6 0 157 272 177 621.6 0 177 273 159 621.6 0 159 274 159 621.6 0 159 275 153 621.6 0 153 276 179 621.6 0 179 277 156 621.6 0 156 278 144 621.6 0 144 279 176 621.6 0 176 280 162 621.6 0 162 281 162 621.6 0 162 282 189 621.6 0 189 283 192 621.6 0 192 284 178 621.6 0 178 285 192 621.6 0 192 286 180 621.6 0 180 287 219 621.6 0 219 288 184 621.6 0 184 289 207 621.6 0 207 290 271 621.6 0 271 291 576 621.6 0 576 292 585 621.6 0 585 293 759 621.6 0 759 294 362 621.6 0 362 295 207 621.6 0 207 296 191 621.6 0 191 297 195 621.6 0 195 298 161 621.6 0 161 299 155 621.6 0 155 300 185 621.6 0 185 301 202 621.6 0 202 Finished in 2.72 s (48 us/read; 1.25 M reads/minute). === Summary === Total reads processed: 56,772 Reads with adapters: 16,114 (28.4%) Reads that were too short: 15 (0.0%) Reads written (passing filters): 16,099 (28.4%) Total basepairs processed: 13,041,318 bp Total written (filtered): 4,376,066 bp (33.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 16114 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 2 1 18 12 0.0 3 0 0 12 19 369 0.0 3 0 365 3 1 20 15619 0.0 4 15386 225 5 3 21 37 0.0 4 7 29 0 1 22 5 0.0 4 3 0 1 1 23 11 0.0 4 0 0 0 10 1 24 2 0.0 4 0 0 0 0 2 68 1 0.0 4 1 87 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 1 115 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 39 0.0 4 0 0 0 0 39 154 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 250 1 0.0 4 0 0 0 1 251 1 0.0 4 0 0 1 253 1 0.0 4 1 257 1 0.0 4 0 0 0 1 283 1 0.0 4 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.58 s (36 us/read; 1.67 M reads/minute). === Summary === Total reads processed: 16,099 Reads with adapters: 3,006 (18.7%) Reads written (passing filters): 16,099 (100.0%) Total basepairs processed: 4,376,066 bp Total written (filtered): 4,347,144 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3006 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 2.5% G: 95.1% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 251.5 0 2 4 2 62.9 0 2 6 1 3.9 0 1 7 1 1.0 0 1 8 3 0.2 0 3 9 1735 0.1 0 63 1672 10 986 0.0 1 32 954 11 270 0.0 1 5 265 12 3 0.0 1 0 3 13 1 0.0 1 0 1 169 1 0.0 1 1 208 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.