This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 65.69 s (26 us/read; 2.33 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 46,822 (1.8%) Reads that were too short: 8,568 (0.3%) Reads written (passing filters): 38,254 (1.5%) Total basepairs processed: 766,381,518 bp Total written (filtered): 8,733,520 bp (1.1%) === Adapter 1 === Sequence: GTAGTG; Type: regular 5'; Length: 6; Trimmed: 46822 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 251 621.6 0 251 7 8628 621.6 0 8628 8 202 621.6 0 202 9 7749 621.6 0 7749 10 38 621.6 0 38 11 33 621.6 0 33 12 23 621.6 0 23 13 28 621.6 0 28 14 25 621.6 0 25 15 32 621.6 0 32 16 50 621.6 0 50 17 37 621.6 0 37 18 16 621.6 0 16 19 20 621.6 0 20 20 21 621.6 0 21 21 28 621.6 0 28 22 41 621.6 0 41 23 36 621.6 0 36 24 32 621.6 0 32 25 31 621.6 0 31 26 97 621.6 0 97 27 50 621.6 0 50 28 110 621.6 0 110 29 38 621.6 0 38 30 49 621.6 0 49 31 59 621.6 0 59 32 26 621.6 0 26 33 26 621.6 0 26 34 36 621.6 0 36 35 31 621.6 0 31 36 18 621.6 0 18 37 39 621.6 0 39 38 30 621.6 0 30 39 43 621.6 0 43 40 36 621.6 0 36 41 13 621.6 0 13 42 55 621.6 0 55 43 51 621.6 0 51 44 29 621.6 0 29 45 27 621.6 0 27 46 26 621.6 0 26 47 19 621.6 0 19 48 25 621.6 0 25 49 42 621.6 0 42 50 33 621.6 0 33 51 57 621.6 0 57 52 54 621.6 0 54 53 43 621.6 0 43 54 29 621.6 0 29 55 35 621.6 0 35 56 44 621.6 0 44 57 26 621.6 0 26 58 42 621.6 0 42 59 41 621.6 0 41 60 44 621.6 0 44 61 31 621.6 0 31 62 39 621.6 0 39 63 37 621.6 0 37 64 22 621.6 0 22 65 35 621.6 0 35 66 32 621.6 0 32 67 57 621.6 0 57 68 71 621.6 0 71 69 628 621.6 0 628 70 177 621.6 0 177 71 704 621.6 0 704 72 39 621.6 0 39 73 47 621.6 0 47 74 25 621.6 0 25 75 49 621.6 0 49 76 53 621.6 0 53 77 56 621.6 0 56 78 65 621.6 0 65 79 71 621.6 0 71 80 39 621.6 0 39 81 54 621.6 0 54 82 43 621.6 0 43 83 56 621.6 0 56 84 52 621.6 0 52 85 39 621.6 0 39 86 47 621.6 0 47 87 34 621.6 0 34 88 36 621.6 0 36 89 52 621.6 0 52 90 46 621.6 0 46 91 46 621.6 0 46 92 47 621.6 0 47 93 41 621.6 0 41 94 37 621.6 0 37 95 30 621.6 0 30 96 53 621.6 0 53 97 60 621.6 0 60 98 66 621.6 0 66 99 69 621.6 0 69 100 51 621.6 0 51 101 52 621.6 0 52 102 44 621.6 0 44 103 28 621.6 0 28 104 32 621.6 0 32 105 47 621.6 0 47 106 35 621.6 0 35 107 62 621.6 0 62 108 46 621.6 0 46 109 56 621.6 0 56 110 40 621.6 0 40 111 46 621.6 0 46 112 73 621.6 0 73 113 39 621.6 0 39 114 85 621.6 0 85 115 84 621.6 0 84 116 107 621.6 0 107 117 39 621.6 0 39 118 46 621.6 0 46 119 35 621.6 0 35 120 38 621.6 0 38 121 30 621.6 0 30 122 42 621.6 0 42 123 33 621.6 0 33 124 52 621.6 0 52 125 29 621.6 0 29 126 77 621.6 0 77 127 93 621.6 0 93 128 56 621.6 0 56 129 63 621.6 0 63 130 95 621.6 0 95 131 69 621.6 0 69 132 284 621.6 0 284 133 85 621.6 0 85 134 254 621.6 0 254 135 84 621.6 0 84 136 86 621.6 0 86 137 129 621.6 0 129 138 302 621.6 0 302 139 350 621.6 0 350 140 2132 621.6 0 2132 141 3922 621.6 0 3922 142 1478 621.6 0 1478 143 455 621.6 0 455 144 1405 621.6 0 1405 145 274 621.6 0 274 146 39 621.6 0 39 147 35 621.6 0 35 148 101 621.6 0 101 149 273 621.6 0 273 150 247 621.6 0 247 151 361 621.6 0 361 152 211 621.6 0 211 153 401 621.6 0 401 154 37 621.6 0 37 155 31 621.6 0 31 156 40 621.6 0 40 157 45 621.6 0 45 158 34 621.6 0 34 159 28 621.6 0 28 160 33 621.6 0 33 161 29 621.6 0 29 162 22 621.6 0 22 163 21 621.6 0 21 164 26 621.6 0 26 165 29 621.6 0 29 166 22 621.6 0 22 167 29 621.6 0 29 168 22 621.6 0 22 169 41 621.6 0 41 170 28 621.6 0 28 171 16 621.6 0 16 172 34 621.6 0 34 173 52 621.6 0 52 174 37 621.6 0 37 175 41 621.6 0 41 176 37 621.6 0 37 177 24 621.6 0 24 178 34 621.6 0 34 179 35 621.6 0 35 180 28 621.6 0 28 181 32 621.6 0 32 182 35 621.6 0 35 183 31 621.6 0 31 184 39 621.6 0 39 185 45 621.6 0 45 186 48 621.6 0 48 187 30 621.6 0 30 188 51 621.6 0 51 189 57 621.6 0 57 190 68 621.6 0 68 191 100 621.6 0 100 192 43 621.6 0 43 193 20 621.6 0 20 194 42 621.6 0 42 195 28 621.6 0 28 196 19 621.6 0 19 197 24 621.6 0 24 198 26 621.6 0 26 199 42 621.6 0 42 200 24 621.6 0 24 201 34 621.6 0 34 202 32 621.6 0 32 203 38 621.6 0 38 204 38 621.6 0 38 205 29 621.6 0 29 206 31 621.6 0 31 207 33 621.6 0 33 208 28 621.6 0 28 209 318 621.6 0 318 210 97 621.6 0 97 211 86 621.6 0 86 212 37 621.6 0 37 213 35 621.6 0 35 214 36 621.6 0 36 215 44 621.6 0 44 216 25 621.6 0 25 217 35 621.6 0 35 218 36 621.6 0 36 219 27 621.6 0 27 220 26 621.6 0 26 221 28 621.6 0 28 222 46 621.6 0 46 223 27 621.6 0 27 224 30 621.6 0 30 225 26 621.6 0 26 226 31 621.6 0 31 227 23 621.6 0 23 228 25 621.6 0 25 229 35 621.6 0 35 230 24 621.6 0 24 231 23 621.6 0 23 232 36 621.6 0 36 233 59 621.6 0 59 234 58 621.6 0 58 235 101 621.6 0 101 236 59 621.6 0 59 237 40 621.6 0 40 238 45 621.6 0 45 239 24 621.6 0 24 240 56 621.6 0 56 241 29 621.6 0 29 242 48 621.6 0 48 243 46 621.6 0 46 244 38 621.6 0 38 245 38 621.6 0 38 246 50 621.6 0 50 247 71 621.6 0 71 248 84 621.6 0 84 249 164 621.6 0 164 250 232 621.6 0 232 251 137 621.6 0 137 252 51 621.6 0 51 253 668 621.6 0 668 254 554 621.6 0 554 255 714 621.6 0 714 256 54 621.6 0 54 257 48 621.6 0 48 258 56 621.6 0 56 259 46 621.6 0 46 260 39 621.6 0 39 261 33 621.6 0 33 262 41 621.6 0 41 263 32 621.6 0 32 264 22 621.6 0 22 265 44 621.6 0 44 266 35 621.6 0 35 267 34 621.6 0 34 268 42 621.6 0 42 269 42 621.6 0 42 270 55 621.6 0 55 271 59 621.6 0 59 272 94 621.6 0 94 273 334 621.6 0 334 274 296 621.6 0 296 275 215 621.6 0 215 276 43 621.6 0 43 277 36 621.6 0 36 278 57 621.6 0 57 279 50 621.6 0 50 280 55 621.6 0 55 281 52 621.6 0 52 282 39 621.6 0 39 283 53 621.6 0 53 284 97 621.6 0 97 285 58 621.6 0 58 286 72 621.6 0 72 287 84 621.6 0 84 288 95 621.6 0 95 289 119 621.6 0 119 290 48 621.6 0 48 291 70 621.6 0 70 292 113 621.6 0 113 293 80 621.6 0 80 294 74 621.6 0 74 295 103 621.6 0 103 296 101 621.6 0 101 297 90 621.6 0 90 298 126 621.6 0 126 299 119 621.6 0 119 300 177 621.6 0 177 301 185 621.6 0 185 Finished in 1.82 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 38,254 Reads with adapters: 8,726 (22.8%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 8,723 (22.8%) Total basepairs processed: 8,733,520 bp Total written (filtered): 2,388,838 bp (27.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8726 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 4 0.0 3 0 0 4 19 112 0.0 3 0 110 2 20 8571 0.0 4 8455 110 3 3 21 20 0.0 4 2 18 22 2 0.0 4 2 23 2 0.0 4 0 0 0 2 57 1 0.0 4 0 0 0 1 107 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 127 8 0.0 4 0 0 0 0 8 128 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 168 1 0.0 4 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.30 s (34 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 8,723 Reads with adapters: 377 (4.3%) Reads written (passing filters): 8,723 (100.0%) Total basepairs processed: 2,388,838 bp Total written (filtered): 2,384,653 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 377 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.1% C: 4.2% G: 91.5% T: 2.1% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 13 136.3 0 13 4 2 34.1 0 2 10 1 0.0 1 0 1 11 346 0.0 1 2 344 12 12 0.0 1 0 12 13 2 0.0 1 0 2 152 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.