This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 67.76 s (27 us/read; 2.25 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 84,513 (3.3%) Reads that were too short: 21,267 (0.8%) Reads written (passing filters): 63,246 (2.5%) Total basepairs processed: 766,381,518 bp Total written (filtered): 17,270,185 bp (2.3%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 84513 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2821 621.6 0 2821 7 727 621.6 0 727 8 26078 621.6 0 26078 9 20601 621.6 0 20601 10 49 621.6 0 49 11 2 621.6 0 2 12 5 621.6 0 5 13 5 621.6 0 5 14 4 621.6 0 4 15 7 621.6 0 7 16 4 621.6 0 4 17 15 621.6 0 15 18 12 621.6 0 12 19 13 621.6 0 13 20 6 621.6 0 6 21 4 621.6 0 4 22 4 621.6 0 4 23 4 621.6 0 4 24 11 621.6 0 11 25 9 621.6 0 9 26 34 621.6 0 34 27 48 621.6 0 48 28 38 621.6 0 38 29 15 621.6 0 15 30 5 621.6 0 5 31 7 621.6 0 7 32 4 621.6 0 4 33 17 621.6 0 17 34 7 621.6 0 7 35 5 621.6 0 5 36 1 621.6 0 1 37 6 621.6 0 6 38 7 621.6 0 7 39 10 621.6 0 10 40 13 621.6 0 13 41 9 621.6 0 9 42 28 621.6 0 28 43 24 621.6 0 24 44 8 621.6 0 8 45 13 621.6 0 13 46 11 621.6 0 11 47 6 621.6 0 6 48 9 621.6 0 9 49 9 621.6 0 9 50 14 621.6 0 14 51 10 621.6 0 10 52 14 621.6 0 14 53 24 621.6 0 24 54 22 621.6 0 22 55 15 621.6 0 15 56 16 621.6 0 16 57 23 621.6 0 23 58 81 621.6 0 81 59 245 621.6 0 245 60 1600 621.6 0 1600 61 1081 621.6 0 1081 62 1222 621.6 0 1222 63 27 621.6 0 27 64 21 621.6 0 21 65 50 621.6 0 50 66 243 621.6 0 243 67 100 621.6 0 100 68 74 621.6 0 74 69 5 621.6 0 5 70 4 621.6 0 4 71 16 621.6 0 16 72 24 621.6 0 24 73 8 621.6 0 8 74 6 621.6 0 6 75 15 621.6 0 15 76 35 621.6 0 35 77 326 621.6 0 326 78 124 621.6 0 124 79 187 621.6 0 187 80 33 621.6 0 33 81 70 621.6 0 70 82 60 621.6 0 60 83 25 621.6 0 25 84 74 621.6 0 74 85 36 621.6 0 36 86 28 621.6 0 28 87 9 621.6 0 9 88 15 621.6 0 15 89 6 621.6 0 6 90 17 621.6 0 17 91 24 621.6 0 24 92 20 621.6 0 20 93 28 621.6 0 28 94 31 621.6 0 31 95 20 621.6 0 20 96 8 621.6 0 8 97 17 621.6 0 17 98 21 621.6 0 21 99 22 621.6 0 22 100 16 621.6 0 16 101 19 621.6 0 19 102 9 621.6 0 9 103 20 621.6 0 20 104 24 621.6 0 24 105 10 621.6 0 10 106 10 621.6 0 10 107 17 621.6 0 17 108 22 621.6 0 22 109 32 621.6 0 32 110 37 621.6 0 37 111 159 621.6 0 159 112 371 621.6 0 371 113 141 621.6 0 141 114 288 621.6 0 288 115 325 621.6 0 325 116 825 621.6 0 825 117 42 621.6 0 42 118 17 621.6 0 17 119 21 621.6 0 21 120 13 621.6 0 13 121 19 621.6 0 19 122 20 621.6 0 20 123 53 621.6 0 53 124 24 621.6 0 24 125 37 621.6 0 37 126 23 621.6 0 23 127 20 621.6 0 20 128 25 621.6 0 25 129 32 621.6 0 32 130 32 621.6 0 32 131 27 621.6 0 27 132 26 621.6 0 26 133 39 621.6 0 39 134 22 621.6 0 22 135 47 621.6 0 47 136 28 621.6 0 28 137 51 621.6 0 51 138 79 621.6 0 79 139 88 621.6 0 88 140 34 621.6 0 34 141 28 621.6 0 28 142 17 621.6 0 17 143 53 621.6 0 53 144 38 621.6 0 38 145 79 621.6 0 79 146 37 621.6 0 37 147 46 621.6 0 46 148 23 621.6 0 23 149 14 621.6 0 14 150 20 621.6 0 20 151 26 621.6 0 26 152 19 621.6 0 19 153 27 621.6 0 27 154 25 621.6 0 25 155 13 621.6 0 13 156 23 621.6 0 23 157 17 621.6 0 17 158 29 621.6 0 29 159 28 621.6 0 28 160 93 621.6 0 93 161 29 621.6 0 29 162 37 621.6 0 37 163 53 621.6 0 53 164 75 621.6 0 75 165 117 621.6 0 117 166 93 621.6 0 93 167 91 621.6 0 91 168 37 621.6 0 37 169 25 621.6 0 25 170 29 621.6 0 29 171 32 621.6 0 32 172 25 621.6 0 25 173 21 621.6 0 21 174 25 621.6 0 25 175 26 621.6 0 26 176 24 621.6 0 24 177 27 621.6 0 27 178 41 621.6 0 41 179 74 621.6 0 74 180 57 621.6 0 57 181 141 621.6 0 141 182 47 621.6 0 47 183 33 621.6 0 33 184 114 621.6 0 114 185 136 621.6 0 136 186 83 621.6 0 83 187 33 621.6 0 33 188 24 621.6 0 24 189 57 621.6 0 57 190 185 621.6 0 185 191 221 621.6 0 221 192 144 621.6 0 144 193 85 621.6 0 85 194 21 621.6 0 21 195 41 621.6 0 41 196 37 621.6 0 37 197 49 621.6 0 49 198 53 621.6 0 53 199 32 621.6 0 32 200 33 621.6 0 33 201 29 621.6 0 29 202 34 621.6 0 34 203 201 621.6 0 201 204 61 621.6 0 61 205 93 621.6 0 93 206 57 621.6 0 57 207 241 621.6 0 241 208 201 621.6 0 201 209 164 621.6 0 164 210 130 621.6 0 130 211 48 621.6 0 48 212 91 621.6 0 91 213 42 621.6 0 42 214 55 621.6 0 55 215 88 621.6 0 88 216 89 621.6 0 89 217 185 621.6 0 185 218 78 621.6 0 78 219 51 621.6 0 51 220 28 621.6 0 28 221 36 621.6 0 36 222 39 621.6 0 39 223 53 621.6 0 53 224 173 621.6 0 173 225 150 621.6 0 150 226 119 621.6 0 119 227 83 621.6 0 83 228 71 621.6 0 71 229 63 621.6 0 63 230 51 621.6 0 51 231 94 621.6 0 94 232 123 621.6 0 123 233 84 621.6 0 84 234 100 621.6 0 100 235 183 621.6 0 183 236 433 621.6 0 433 237 416 621.6 0 416 238 380 621.6 0 380 239 149 621.6 0 149 240 76 621.6 0 76 241 66 621.6 0 66 242 79 621.6 0 79 243 139 621.6 0 139 244 265 621.6 0 265 245 3736 621.6 0 3736 246 1655 621.6 0 1655 247 3217 621.6 0 3217 248 53 621.6 0 53 249 44 621.6 0 44 250 38 621.6 0 38 251 47 621.6 0 47 252 54 621.6 0 54 253 44 621.6 0 44 254 93 621.6 0 93 255 88 621.6 0 88 256 67 621.6 0 67 257 43 621.6 0 43 258 49 621.6 0 49 259 92 621.6 0 92 260 219 621.6 0 219 261 207 621.6 0 207 262 279 621.6 0 279 263 181 621.6 0 181 264 186 621.6 0 186 265 1879 621.6 0 1879 266 262 621.6 0 262 267 219 621.6 0 219 268 70 621.6 0 70 269 36 621.6 0 36 270 196 621.6 0 196 271 219 621.6 0 219 272 290 621.6 0 290 273 78 621.6 0 78 274 43 621.6 0 43 275 35 621.6 0 35 276 86 621.6 0 86 277 85 621.6 0 85 278 163 621.6 0 163 279 77 621.6 0 77 280 86 621.6 0 86 281 77 621.6 0 77 282 82 621.6 0 82 283 80 621.6 0 80 284 144 621.6 0 144 285 112 621.6 0 112 286 94 621.6 0 94 287 90 621.6 0 90 288 51 621.6 0 51 289 47 621.6 0 47 290 46 621.6 0 46 291 67 621.6 0 67 292 76 621.6 0 76 293 83 621.6 0 83 294 61 621.6 0 61 295 70 621.6 0 70 296 73 621.6 0 73 297 69 621.6 0 69 298 115 621.6 0 115 299 90 621.6 0 90 300 81 621.6 0 81 301 81 621.6 0 81 Finished in 3.06 s (48 us/read; 1.24 M reads/minute). === Summary === Total reads processed: 63,246 Reads with adapters: 26,145 (41.3%) Reads that were too short: 112 (0.2%) Reads written (passing filters): 26,033 (41.2%) Total basepairs processed: 17,270,185 bp Total written (filtered): 7,169,209 bp (41.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 26145 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 6 0.0 2 0 0 0 6 15 17 0.0 3 0 0 11 6 16 267 0.0 3 0 242 16 9 17 25397 0.0 3 23734 1394 207 62 18 93 0.0 3 14 52 10 17 19 70 0.0 3 1 0 40 29 20 74 0.0 3 0 0 0 74 22 3 0.0 3 1 0 0 2 26 7 0.0 3 0 0 0 7 27 3 0.0 3 1 0 1 1 28 4 0.0 3 0 0 0 4 29 3 0.0 3 0 0 0 3 30 1 0.0 3 0 0 0 1 31 1 0.0 3 0 0 1 33 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 3 0.0 3 0 0 0 3 55 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 57 2 0.0 3 0 0 0 2 60 7 0.0 3 0 0 0 7 61 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 63 3 0.0 3 0 0 0 3 66 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 75 2 0.0 3 0 0 0 2 76 1 0.0 3 0 0 0 1 77 11 0.0 3 0 0 0 11 78 20 0.0 3 0 0 0 20 80 7 0.0 3 0 0 0 7 81 1 0.0 3 0 0 0 1 83 2 0.0 3 0 0 1 1 86 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 102 2 0.0 3 0 0 0 2 103 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 106 3 0.0 3 0 0 1 2 109 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 116 1 0.0 3 0 0 0 1 121 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 126 1 0.0 3 0 0 0 1 128 2 0.0 3 0 0 1 1 130 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 137 5 0.0 3 0 0 0 5 138 1 0.0 3 0 0 0 1 140 4 0.0 3 0 0 0 4 141 2 0.0 3 0 0 0 2 142 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 1 152 2 0.0 3 0 0 0 2 154 1 0.0 3 0 0 1 156 5 0.0 3 0 0 0 5 158 2 0.0 3 0 0 0 2 160 1 0.0 3 0 0 0 1 161 1 0.0 3 0 0 0 1 162 1 0.0 3 0 0 0 1 163 2 0.0 3 0 0 0 2 166 2 0.0 3 0 0 0 2 169 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 179 4 0.0 3 0 0 1 3 182 3 0.0 3 0 0 0 3 185 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 192 2 0.0 3 0 0 0 2 206 1 0.0 3 0 0 0 1 210 1 0.0 3 0 0 0 1 212 2 0.0 3 0 0 0 2 216 1 0.0 3 0 0 0 1 217 17 0.0 3 0 0 1 16 218 3 0.0 3 0 0 0 3 221 3 0.0 3 0 0 0 3 227 1 0.0 3 0 0 0 1 228 4 0.0 3 0 0 0 4 229 1 0.0 3 0 0 0 1 236 1 0.0 3 0 0 0 1 238 1 0.0 3 0 0 0 1 244 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 0 1 268 1 0.0 3 0 0 0 1 270 1 0.0 3 0 0 0 1 278 1 0.0 3 0 0 0 1 279 2 0.0 3 0 0 0 2 280 4 0.0 3 0 0 0 4 281 2 0.0 3 0 0 0 2 282 5 0.0 3 0 0 0 5 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.90 s (35 us/read; 1.74 M reads/minute). === Summary === Total reads processed: 26,033 Reads with adapters: 237 (0.9%) Reads written (passing filters): 26,033 (100.0%) Total basepairs processed: 7,169,209 bp Total written (filtered): 7,168,428 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 237 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 49.4% C: 13.5% G: 33.3% T: 3.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 170 406.8 0 170 4 64 101.7 0 64 5 3 25.4 0 3