This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 67.61 s (27 us/read; 2.26 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 76,937 (3.0%) Reads that were too short: 17,816 (0.7%) Reads written (passing filters): 59,121 (2.3%) Total basepairs processed: 766,381,518 bp Total written (filtered): 16,722,105 bp (2.2%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 76937 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3433 621.6 0 3433 7 558 621.6 0 558 8 23482 621.6 0 23482 9 25937 621.6 0 25937 10 22 621.6 0 22 11 8 621.6 0 8 12 6 621.6 0 6 13 1 621.6 0 1 14 4 621.6 0 4 15 3 621.6 0 3 17 1 621.6 0 1 18 3 621.6 0 3 19 2 621.6 0 2 20 2 621.6 0 2 22 1 621.6 0 1 23 6 621.6 0 6 24 3 621.6 0 3 25 1 621.6 0 1 26 3 621.6 0 3 27 6 621.6 0 6 28 4 621.6 0 4 29 4 621.6 0 4 30 3 621.6 0 3 31 1 621.6 0 1 32 9 621.6 0 9 33 8 621.6 0 8 34 3 621.6 0 3 35 2 621.6 0 2 36 2 621.6 0 2 37 6 621.6 0 6 38 3 621.6 0 3 39 6 621.6 0 6 40 5 621.6 0 5 41 6 621.6 0 6 42 4 621.6 0 4 43 12 621.6 0 12 44 4 621.6 0 4 45 8 621.6 0 8 46 8 621.6 0 8 47 2 621.6 0 2 48 6 621.6 0 6 49 6 621.6 0 6 50 6 621.6 0 6 51 4 621.6 0 4 52 8 621.6 0 8 53 5 621.6 0 5 54 7 621.6 0 7 55 6 621.6 0 6 56 9 621.6 0 9 57 8 621.6 0 8 58 32 621.6 0 32 59 119 621.6 0 119 60 536 621.6 0 536 61 293 621.6 0 293 62 452 621.6 0 452 63 4 621.6 0 4 64 14 621.6 0 14 65 14 621.6 0 14 66 157 621.6 0 157 67 78 621.6 0 78 68 22 621.6 0 22 69 6 621.6 0 6 70 5 621.6 0 5 71 8 621.6 0 8 72 12 621.6 0 12 73 6 621.6 0 6 74 5 621.6 0 5 75 11 621.6 0 11 76 9 621.6 0 9 77 150 621.6 0 150 78 83 621.6 0 83 79 113 621.6 0 113 80 18 621.6 0 18 81 89 621.6 0 89 82 16 621.6 0 16 83 14 621.6 0 14 84 7 621.6 0 7 85 22 621.6 0 22 86 40 621.6 0 40 87 7 621.6 0 7 88 4 621.6 0 4 89 5 621.6 0 5 90 17 621.6 0 17 91 11 621.6 0 11 92 12 621.6 0 12 93 15 621.6 0 15 94 9 621.6 0 9 95 11 621.6 0 11 96 14 621.6 0 14 97 21 621.6 0 21 98 19 621.6 0 19 99 15 621.6 0 15 100 11 621.6 0 11 101 11 621.6 0 11 102 15 621.6 0 15 103 13 621.6 0 13 104 26 621.6 0 26 105 11 621.6 0 11 106 15 621.6 0 15 107 14 621.6 0 14 108 15 621.6 0 15 109 16 621.6 0 16 110 13 621.6 0 13 111 59 621.6 0 59 112 146 621.6 0 146 113 44 621.6 0 44 114 95 621.6 0 95 115 127 621.6 0 127 116 199 621.6 0 199 117 45 621.6 0 45 118 11 621.6 0 11 119 11 621.6 0 11 120 10 621.6 0 10 121 9 621.6 0 9 122 7 621.6 0 7 123 43 621.6 0 43 124 16 621.6 0 16 125 14 621.6 0 14 126 9 621.6 0 9 127 14 621.6 0 14 128 6 621.6 0 6 129 10 621.6 0 10 130 12 621.6 0 12 131 15 621.6 0 15 132 15 621.6 0 15 133 25 621.6 0 25 134 9 621.6 0 9 135 44 621.6 0 44 136 13 621.6 0 13 137 25 621.6 0 25 138 32 621.6 0 32 139 34 621.6 0 34 140 28 621.6 0 28 141 17 621.6 0 17 142 22 621.6 0 22 143 20 621.6 0 20 144 22 621.6 0 22 145 22 621.6 0 22 146 10 621.6 0 10 147 9 621.6 0 9 148 7 621.6 0 7 149 13 621.6 0 13 150 11 621.6 0 11 151 22 621.6 0 22 152 16 621.6 0 16 153 21 621.6 0 21 154 12 621.6 0 12 155 16 621.6 0 16 156 18 621.6 0 18 157 11 621.6 0 11 158 10 621.6 0 10 159 24 621.6 0 24 160 91 621.6 0 91 161 16 621.6 0 16 162 19 621.6 0 19 163 18 621.6 0 18 164 20 621.6 0 20 165 72 621.6 0 72 166 41 621.6 0 41 167 56 621.6 0 56 168 18 621.6 0 18 169 11 621.6 0 11 170 13 621.6 0 13 171 15 621.6 0 15 172 15 621.6 0 15 173 14 621.6 0 14 174 13 621.6 0 13 175 8 621.6 0 8 176 12 621.6 0 12 177 18 621.6 0 18 178 21 621.6 0 21 179 40 621.6 0 40 180 35 621.6 0 35 181 88 621.6 0 88 182 11 621.6 0 11 183 11 621.6 0 11 184 31 621.6 0 31 185 50 621.6 0 50 186 30 621.6 0 30 187 19 621.6 0 19 188 17 621.6 0 17 189 44 621.6 0 44 190 98 621.6 0 98 191 111 621.6 0 111 192 89 621.6 0 89 193 67 621.6 0 67 194 17 621.6 0 17 195 19 621.6 0 19 196 19 621.6 0 19 197 30 621.6 0 30 198 21 621.6 0 21 199 15 621.6 0 15 200 16 621.6 0 16 201 20 621.6 0 20 202 21 621.6 0 21 203 171 621.6 0 171 204 27 621.6 0 27 205 79 621.6 0 79 206 28 621.6 0 28 207 140 621.6 0 140 208 101 621.6 0 101 209 73 621.6 0 73 210 59 621.6 0 59 211 34 621.6 0 34 212 101 621.6 0 101 213 17 621.6 0 17 214 48 621.6 0 48 215 29 621.6 0 29 216 43 621.6 0 43 217 57 621.6 0 57 218 40 621.6 0 40 219 32 621.6 0 32 220 16 621.6 0 16 221 30 621.6 0 30 222 22 621.6 0 22 223 23 621.6 0 23 224 50 621.6 0 50 225 58 621.6 0 58 226 38 621.6 0 38 227 23 621.6 0 23 228 33 621.6 0 33 229 24 621.6 0 24 230 31 621.6 0 31 231 52 621.6 0 52 232 91 621.6 0 91 233 47 621.6 0 47 234 53 621.6 0 53 235 138 621.6 0 138 236 195 621.6 0 195 237 194 621.6 0 194 238 178 621.6 0 178 239 83 621.6 0 83 240 52 621.6 0 52 241 66 621.6 0 66 242 81 621.6 0 81 243 126 621.6 0 126 244 286 621.6 0 286 245 4339 621.6 0 4339 246 1966 621.6 0 1966 247 3696 621.6 0 3696 248 37 621.6 0 37 249 24 621.6 0 24 250 33 621.6 0 33 251 18 621.6 0 18 252 36 621.6 0 36 253 35 621.6 0 35 254 17 621.6 0 17 255 26 621.6 0 26 256 22 621.6 0 22 257 19 621.6 0 19 258 26 621.6 0 26 259 49 621.6 0 49 260 126 621.6 0 126 261 104 621.6 0 104 262 140 621.6 0 140 263 100 621.6 0 100 264 113 621.6 0 113 265 2063 621.6 0 2063 266 204 621.6 0 204 267 132 621.6 0 132 268 32 621.6 0 32 269 19 621.6 0 19 270 109 621.6 0 109 271 94 621.6 0 94 272 86 621.6 0 86 273 23 621.6 0 23 274 25 621.6 0 25 275 15 621.6 0 15 276 60 621.6 0 60 277 49 621.6 0 49 278 58 621.6 0 58 279 37 621.6 0 37 280 46 621.6 0 46 281 29 621.6 0 29 282 30 621.6 0 30 283 31 621.6 0 31 284 39 621.6 0 39 285 43 621.6 0 43 286 51 621.6 0 51 287 55 621.6 0 55 288 33 621.6 0 33 289 25 621.6 0 25 290 21 621.6 0 21 291 28 621.6 0 28 292 23 621.6 0 23 293 37 621.6 0 37 294 30 621.6 0 30 295 48 621.6 0 48 296 24 621.6 0 24 297 28 621.6 0 28 298 52 621.6 0 52 299 39 621.6 0 39 300 54 621.6 0 54 301 28 621.6 0 28 Finished in 3.16 s (53 us/read; 1.12 M reads/minute). === Summary === Total reads processed: 59,121 Reads with adapters: 30,340 (51.3%) Reads that were too short: 8 (0.0%) Reads written (passing filters): 30,332 (51.3%) Total basepairs processed: 16,722,105 bp Total written (filtered): 8,253,948 bp (49.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 30340 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 0 2 18 11 0.0 3 0 0 11 19 383 0.0 3 0 380 3 20 26711 0.0 4 26341 353 16 0 1 21 91 0.0 4 21 69 1 22 3082 0.0 4 3023 50 5 2 2 23 35 0.0 4 3 10 0 19 3 24 1 0.0 4 0 1 25 5 0.0 4 1 0 0 4 79 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 103 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 7 0.0 4 0 0 0 0 7 153 1 0.0 4 0 0 0 0 1 174 1 0.0 4 1 185 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 235 1 0.0 4 0 0 0 0 1 266 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.11 s (37 us/read; 1.64 M reads/minute). === Summary === Total reads processed: 30,332 Reads with adapters: 6,591 (21.7%) Reads written (passing filters): 30,332 (100.0%) Total basepairs processed: 8,253,948 bp Total written (filtered): 8,172,482 bp (99.0%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6591 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 0.7% C: 2.2% G: 94.8% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 8 473.9 0 8 4 7 118.5 0 7 5 3 29.6 0 3 6 1 7.4 0 1 7 2 1.9 0 2 8 2 0.5 0 2 9 4354 0.1 0 143 4211 10 1552 0.0 1 62 1490 11 556 0.0 1 3 553 12 10 0.0 1 0 10 13 2 0.0 1 0 2 150 4 0.0 1 0 4 153 1 0.0 1 0 1 169 3 0.0 1 3 208 4 0.0 1 4 223 74 0.0 1 70 4 224 3 0.0 1 3 225 1 0.0 1 1 226 4 0.0 1 4 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.