This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 65.26 s (26 us/read; 2.34 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 140,928 (5.5%) Reads that were too short: 75,956 (3.0%) Reads written (passing filters): 64,972 (2.6%) Total basepairs processed: 766,381,518 bp Total written (filtered): 14,843,299 bp (1.9%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 140928 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 365 621.6 0 365 7 13837 621.6 0 13837 8 10681 621.6 0 10681 9 279 621.6 0 279 10 235 621.6 0 235 11 5232 621.6 0 5232 12 83 621.6 0 83 13 79 621.6 0 79 14 98 621.6 0 98 15 113 621.6 0 113 16 109 621.6 0 109 17 99 621.6 0 99 18 64 621.6 0 64 19 61 621.6 0 61 20 78 621.6 0 78 21 68 621.6 0 68 22 56 621.6 0 56 23 80 621.6 0 80 24 78 621.6 0 78 25 72 621.6 0 72 26 68 621.6 0 68 27 77 621.6 0 77 28 62 621.6 0 62 29 71 621.6 0 71 30 82 621.6 0 82 31 79 621.6 0 79 32 85 621.6 0 85 33 75 621.6 0 75 34 85 621.6 0 85 35 97 621.6 0 97 36 80 621.6 0 80 37 93 621.6 0 93 38 104 621.6 0 104 39 80 621.6 0 80 40 70 621.6 0 70 41 96 621.6 0 96 42 66 621.6 0 66 43 111 621.6 0 111 44 76 621.6 0 76 45 101 621.6 0 101 46 83 621.6 0 83 47 68 621.6 0 68 48 94 621.6 0 94 49 71 621.6 0 71 50 83 621.6 0 83 51 81 621.6 0 81 52 200 621.6 0 200 53 182 621.6 0 182 54 281 621.6 0 281 55 93 621.6 0 93 56 80 621.6 0 80 57 113 621.6 0 113 58 79 621.6 0 79 59 96 621.6 0 96 60 191 621.6 0 191 61 92 621.6 0 92 62 85 621.6 0 85 63 104 621.6 0 104 64 122 621.6 0 122 65 100 621.6 0 100 66 105 621.6 0 105 67 91 621.6 0 91 68 120 621.6 0 120 69 99 621.6 0 99 70 103 621.6 0 103 71 134 621.6 0 134 72 297 621.6 0 297 73 188 621.6 0 188 74 207 621.6 0 207 75 93 621.6 0 93 76 73 621.6 0 73 77 73 621.6 0 73 78 99 621.6 0 99 79 132 621.6 0 132 80 112 621.6 0 112 81 241 621.6 0 241 82 123 621.6 0 123 83 99 621.6 0 99 84 127 621.6 0 127 85 111 621.6 0 111 86 116 621.6 0 116 87 97 621.6 0 97 88 113 621.6 0 113 89 91 621.6 0 91 90 82 621.6 0 82 91 97 621.6 0 97 92 91 621.6 0 91 93 112 621.6 0 112 94 95 621.6 0 95 95 93 621.6 0 93 96 106 621.6 0 106 97 101 621.6 0 101 98 133 621.6 0 133 99 135 621.6 0 135 100 119 621.6 0 119 101 141 621.6 0 141 102 141 621.6 0 141 103 161 621.6 0 161 104 178 621.6 0 178 105 158 621.6 0 158 106 144 621.6 0 144 107 150 621.6 0 150 108 127 621.6 0 127 109 123 621.6 0 123 110 148 621.6 0 148 111 192 621.6 0 192 112 121 621.6 0 121 113 132 621.6 0 132 114 140 621.6 0 140 115 122 621.6 0 122 116 128 621.6 0 128 117 126 621.6 0 126 118 128 621.6 0 128 119 124 621.6 0 124 120 124 621.6 0 124 121 152 621.6 0 152 122 180 621.6 0 180 123 155 621.6 0 155 124 329 621.6 0 329 125 359 621.6 0 359 126 340 621.6 0 340 127 112 621.6 0 112 128 123 621.6 0 123 129 110 621.6 0 110 130 152 621.6 0 152 131 137 621.6 0 137 132 189 621.6 0 189 133 305 621.6 0 305 134 317 621.6 0 317 135 176 621.6 0 176 136 141 621.6 0 141 137 189 621.6 0 189 138 175 621.6 0 175 139 197 621.6 0 197 140 139 621.6 0 139 141 140 621.6 0 140 142 129 621.6 0 129 143 133 621.6 0 133 144 130 621.6 0 130 145 180 621.6 0 180 146 251 621.6 0 251 147 415 621.6 0 415 148 292 621.6 0 292 149 411 621.6 0 411 150 230 621.6 0 230 151 180 621.6 0 180 152 183 621.6 0 183 153 180 621.6 0 180 154 173 621.6 0 173 155 147 621.6 0 147 156 251 621.6 0 251 157 265 621.6 0 265 158 2209 621.6 0 2209 159 280 621.6 0 280 160 266 621.6 0 266 161 229 621.6 0 229 162 225 621.6 0 225 163 199 621.6 0 199 164 181 621.6 0 181 165 161 621.6 0 161 166 252 621.6 0 252 167 259 621.6 0 259 168 354 621.6 0 354 169 711 621.6 0 711 170 1024 621.6 0 1024 171 1455 621.6 0 1455 172 786 621.6 0 786 173 275 621.6 0 275 174 190 621.6 0 190 175 180 621.6 0 180 176 152 621.6 0 152 177 145 621.6 0 145 178 161 621.6 0 161 179 200 621.6 0 200 180 267 621.6 0 267 181 290 621.6 0 290 182 295 621.6 0 295 183 241 621.6 0 241 184 229 621.6 0 229 185 234 621.6 0 234 186 166 621.6 0 166 187 380 621.6 0 380 188 217 621.6 0 217 189 306 621.6 0 306 190 146 621.6 0 146 191 140 621.6 0 140 192 172 621.6 0 172 193 215 621.6 0 215 194 214 621.6 0 214 195 190 621.6 0 190 196 159 621.6 0 159 197 137 621.6 0 137 198 159 621.6 0 159 199 180 621.6 0 180 200 178 621.6 0 178 201 157 621.6 0 157 202 171 621.6 0 171 203 294 621.6 0 294 204 239 621.6 0 239 205 256 621.6 0 256 206 159 621.6 0 159 207 138 621.6 0 138 208 143 621.6 0 143 209 204 621.6 0 204 210 168 621.6 0 168 211 184 621.6 0 184 212 160 621.6 0 160 213 228 621.6 0 228 214 711 621.6 0 711 215 879 621.6 0 879 216 987 621.6 0 987 217 595 621.6 0 595 218 388 621.6 0 388 219 405 621.6 0 405 220 219 621.6 0 219 221 201 621.6 0 201 222 239 621.6 0 239 223 308 621.6 0 308 224 405 621.6 0 405 225 885 621.6 0 885 226 3956 621.6 0 3956 227 8237 621.6 0 8237 228 6957 621.6 0 6957 229 5832 621.6 0 5832 230 1092 621.6 0 1092 231 1137 621.6 0 1137 232 900 621.6 0 900 233 479 621.6 0 479 234 160 621.6 0 160 235 145 621.6 0 145 236 182 621.6 0 182 237 303 621.6 0 303 238 498 621.6 0 498 239 530 621.6 0 530 240 333 621.6 0 333 241 149 621.6 0 149 242 132 621.6 0 132 243 155 621.6 0 155 244 178 621.6 0 178 245 179 621.6 0 179 246 180 621.6 0 180 247 181 621.6 0 181 248 225 621.6 0 225 249 276 621.6 0 276 250 234 621.6 0 234 251 159 621.6 0 159 252 152 621.6 0 152 253 155 621.6 0 155 254 196 621.6 0 196 255 234 621.6 0 234 256 302 621.6 0 302 257 369 621.6 0 369 258 265 621.6 0 265 259 379 621.6 0 379 260 274 621.6 0 274 261 359 621.6 0 359 262 346 621.6 0 346 263 477 621.6 0 477 264 2230 621.6 0 2230 265 3338 621.6 0 3338 266 4676 621.6 0 4676 267 2681 621.6 0 2681 268 2089 621.6 0 2089 269 590 621.6 0 590 270 483 621.6 0 483 271 368 621.6 0 368 272 334 621.6 0 334 273 523 621.6 0 523 274 597 621.6 0 597 275 604 621.6 0 604 276 538 621.6 0 538 277 304 621.6 0 304 278 312 621.6 0 312 279 496 621.6 0 496 280 517 621.6 0 517 281 500 621.6 0 500 282 245 621.6 0 245 283 222 621.6 0 222 284 259 621.6 0 259 285 313 621.6 0 313 286 433 621.6 0 433 287 465 621.6 0 465 288 287 621.6 0 287 289 314 621.6 0 314 290 313 621.6 0 313 291 1707 621.6 0 1707 292 1570 621.6 0 1570 293 2039 621.6 0 2039 294 358 621.6 0 358 295 187 621.6 0 187 296 170 621.6 0 170 297 135 621.6 0 135 298 151 621.6 0 151 299 162 621.6 0 162 300 178 621.6 0 178 301 405 621.6 0 405 Finished in 2.84 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 64,972 Reads with adapters: 13,862 (21.3%) Reads that were too short: 119 (0.2%) Reads written (passing filters): 13,743 (21.2%) Total basepairs processed: 14,843,299 bp Total written (filtered): 3,776,108 bp (25.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 13862 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 128 0.0 3 0 108 9 11 17 13269 0.0 3 12444 669 124 32 18 36 0.0 3 7 22 5 2 19 35 0.0 3 1 0 20 14 20 85 0.0 3 0 0 2 83 21 2 0.0 3 0 0 0 2 22 3 0.0 3 0 0 0 3 23 8 0.0 3 0 0 0 8 24 2 0.0 3 0 0 0 2 25 4 0.0 3 0 0 1 3 26 2 0.0 3 0 0 0 2 29 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 31 2 0.0 3 0 0 0 2 32 2 0.0 3 0 0 0 2 33 2 0.0 3 1 0 0 1 34 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 36 1 0.0 3 0 0 0 1 37 1 0.0 3 0 0 0 1 38 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 40 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 46 4 0.0 3 0 0 0 4 47 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 3 0.0 3 0 0 0 3 51 1 0.0 3 0 0 0 1 52 2 0.0 3 0 0 0 2 53 2 0.0 3 0 0 1 1 54 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 58 2 0.0 3 0 0 0 2 60 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 62 2 0.0 3 0 0 0 2 63 2 0.0 3 0 0 0 2 64 1 0.0 3 0 0 0 1 65 2 0.0 3 0 0 0 2 66 1 0.0 3 0 0 0 1 67 15 0.0 3 0 0 0 15 68 2 0.0 3 0 0 0 2 69 1 0.0 3 0 0 0 1 70 6 0.0 3 0 0 0 6 71 1 0.0 3 0 0 0 1 72 2 0.0 3 0 0 0 2 73 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 77 2 0.0 3 0 0 0 2 78 1 0.0 3 0 0 0 1 79 2 0.0 3 0 0 0 2 80 2 0.0 3 0 0 0 2 81 2 0.0 3 0 0 0 2 82 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 87 2 0.0 3 0 0 0 2 88 1 0.0 3 0 0 0 1 89 3 0.0 3 0 0 0 3 91 1 0.0 3 0 0 0 1 92 2 0.0 3 0 0 0 2 94 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 113 4 0.0 3 0 0 0 4 114 3 0.0 3 0 0 1 2 115 1 0.0 3 0 0 0 1 116 1 0.0 3 0 1 117 1 0.0 3 0 0 0 1 118 3 0.0 3 0 1 0 2 119 2 0.0 3 0 0 1 1 121 2 0.0 3 0 0 0 2 123 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 127 5 0.0 3 0 0 0 5 129 1 0.0 3 0 0 0 1 132 3 0.0 3 0 0 0 3 133 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 142 2 0.0 3 0 0 1 1 143 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 157 16 0.0 3 0 0 0 16 160 3 0.0 3 0 0 0 3 162 18 0.0 3 0 0 0 18 163 3 0.0 3 0 0 0 3 164 1 0.0 3 0 0 0 1 165 5 0.0 3 0 0 0 5 166 1 0.0 3 0 0 0 1 168 2 0.0 3 0 0 0 2 169 2 0.0 3 0 0 0 2 170 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 177 2 0.0 3 0 0 0 2 179 1 0.0 3 0 0 0 1 180 1 0.0 3 0 0 0 1 181 3 0.0 3 0 0 0 3 182 31 0.0 3 0 0 0 31 183 10 0.0 3 0 0 0 10 184 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 186 4 0.0 3 0 0 0 4 188 1 0.0 3 0 0 0 1 189 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 1 205 2 0.0 3 0 0 0 2 208 1 0.0 3 0 0 0 1 213 2 0.0 3 0 0 0 2 215 1 0.0 3 0 0 0 1 218 4 0.0 3 0 0 0 4 226 1 0.0 3 0 0 0 1 240 1 0.0 3 0 0 0 1 241 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 261 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 271 1 0.0 3 0 0 0 1 272 1 0.0 3 0 0 0 1 277 1 0.0 3 0 0 0 1 278 2 0.0 3 0 0 0 2 280 2 0.0 3 0 0 0 2 281 1 0.0 3 0 0 0 1 282 4 0.0 3 0 0 0 4 292 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.48 s (35 us/read; 1.72 M reads/minute). === Summary === Total reads processed: 13,743 Reads with adapters: 25 (0.2%) Reads written (passing filters): 13,743 (100.0%) Total basepairs processed: 3,776,108 bp Total written (filtered): 3,776,032 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 25 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 40.0% C: 32.0% G: 24.0% T: 4.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 24 214.7 0 24 4 1 53.7 0 1