This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACAACC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 64.54 s (25 us/read; 2.37 M reads/minute). === Summary === Total reads processed: 2,546,118 Reads with adapters: 101,413 (4.0%) Reads that were too short: 40,587 (1.6%) Reads written (passing filters): 60,826 (2.4%) Total basepairs processed: 766,381,518 bp Total written (filtered): 14,398,061 bp (1.9%) === Adapter 1 === Sequence: ACAACC; Type: regular 5'; Length: 6; Trimmed: 101413 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 289 621.6 0 289 7 11342 621.6 0 11342 8 14313 621.6 0 14313 9 154 621.6 0 154 10 198 621.6 0 198 11 5084 621.6 0 5084 12 85 621.6 0 85 13 89 621.6 0 89 14 95 621.6 0 95 15 83 621.6 0 83 16 87 621.6 0 87 17 107 621.6 0 107 18 83 621.6 0 83 19 76 621.6 0 76 20 85 621.6 0 85 21 69 621.6 0 69 22 82 621.6 0 82 23 97 621.6 0 97 24 95 621.6 0 95 25 99 621.6 0 99 26 79 621.6 0 79 27 79 621.6 0 79 28 68 621.6 0 68 29 77 621.6 0 77 30 84 621.6 0 84 31 104 621.6 0 104 32 92 621.6 0 92 33 74 621.6 0 74 34 93 621.6 0 93 35 115 621.6 0 115 36 101 621.6 0 101 37 89 621.6 0 89 38 108 621.6 0 108 39 91 621.6 0 91 40 71 621.6 0 71 41 97 621.6 0 97 42 64 621.6 0 64 43 62 621.6 0 62 44 91 621.6 0 91 45 104 621.6 0 104 46 79 621.6 0 79 47 84 621.6 0 84 48 83 621.6 0 83 49 75 621.6 0 75 50 90 621.6 0 90 51 85 621.6 0 85 52 156 621.6 0 156 53 103 621.6 0 103 54 119 621.6 0 119 55 67 621.6 0 67 56 99 621.6 0 99 57 79 621.6 0 79 58 77 621.6 0 77 59 102 621.6 0 102 60 179 621.6 0 179 61 110 621.6 0 110 62 68 621.6 0 68 63 92 621.6 0 92 64 119 621.6 0 119 65 104 621.6 0 104 66 130 621.6 0 130 67 76 621.6 0 76 68 119 621.6 0 119 69 122 621.6 0 122 70 117 621.6 0 117 71 111 621.6 0 111 72 140 621.6 0 140 73 124 621.6 0 124 74 132 621.6 0 132 75 104 621.6 0 104 76 95 621.6 0 95 77 105 621.6 0 105 78 121 621.6 0 121 79 109 621.6 0 109 80 159 621.6 0 159 81 290 621.6 0 290 82 156 621.6 0 156 83 148 621.6 0 148 84 179 621.6 0 179 85 161 621.6 0 161 86 168 621.6 0 168 87 137 621.6 0 137 88 157 621.6 0 157 89 99 621.6 0 99 90 100 621.6 0 100 91 131 621.6 0 131 92 121 621.6 0 121 93 121 621.6 0 121 94 97 621.6 0 97 95 104 621.6 0 104 96 129 621.6 0 129 97 93 621.6 0 93 98 168 621.6 0 168 99 186 621.6 0 186 100 130 621.6 0 130 101 157 621.6 0 157 102 140 621.6 0 140 103 187 621.6 0 187 104 124 621.6 0 124 105 128 621.6 0 128 106 122 621.6 0 122 107 164 621.6 0 164 108 112 621.6 0 112 109 136 621.6 0 136 110 151 621.6 0 151 111 128 621.6 0 128 112 122 621.6 0 122 113 118 621.6 0 118 114 118 621.6 0 118 115 117 621.6 0 117 116 158 621.6 0 158 117 126 621.6 0 126 118 118 621.6 0 118 119 118 621.6 0 118 120 111 621.6 0 111 121 129 621.6 0 129 122 143 621.6 0 143 123 136 621.6 0 136 124 211 621.6 0 211 125 195 621.6 0 195 126 179 621.6 0 179 127 128 621.6 0 128 128 125 621.6 0 125 129 117 621.6 0 117 130 136 621.6 0 136 131 127 621.6 0 127 132 174 621.6 0 174 133 147 621.6 0 147 134 246 621.6 0 246 135 136 621.6 0 136 136 149 621.6 0 149 137 141 621.6 0 141 138 122 621.6 0 122 139 162 621.6 0 162 140 132 621.6 0 132 141 111 621.6 0 111 142 109 621.6 0 109 143 130 621.6 0 130 144 116 621.6 0 116 145 143 621.6 0 143 146 170 621.6 0 170 147 339 621.6 0 339 148 183 621.6 0 183 149 285 621.6 0 285 150 136 621.6 0 136 151 121 621.6 0 121 152 125 621.6 0 125 153 163 621.6 0 163 154 175 621.6 0 175 155 153 621.6 0 153 156 176 621.6 0 176 157 219 621.6 0 219 158 3032 621.6 0 3032 159 162 621.6 0 162 160 136 621.6 0 136 161 128 621.6 0 128 162 159 621.6 0 159 163 126 621.6 0 126 164 138 621.6 0 138 165 135 621.6 0 135 166 155 621.6 0 155 167 152 621.6 0 152 168 151 621.6 0 151 169 363 621.6 0 363 170 588 621.6 0 588 171 834 621.6 0 834 172 451 621.6 0 451 173 147 621.6 0 147 174 156 621.6 0 156 175 144 621.6 0 144 176 127 621.6 0 127 177 117 621.6 0 117 178 145 621.6 0 145 179 168 621.6 0 168 180 157 621.6 0 157 181 157 621.6 0 157 182 151 621.6 0 151 183 163 621.6 0 163 184 173 621.6 0 173 185 171 621.6 0 171 186 114 621.6 0 114 187 196 621.6 0 196 188 227 621.6 0 227 189 175 621.6 0 175 190 158 621.6 0 158 191 131 621.6 0 131 192 196 621.6 0 196 193 178 621.6 0 178 194 194 621.6 0 194 195 169 621.6 0 169 196 144 621.6 0 144 197 140 621.6 0 140 198 163 621.6 0 163 199 158 621.6 0 158 200 151 621.6 0 151 201 138 621.6 0 138 202 123 621.6 0 123 203 199 621.6 0 199 204 178 621.6 0 178 205 191 621.6 0 191 206 141 621.6 0 141 207 133 621.6 0 133 208 131 621.6 0 131 209 134 621.6 0 134 210 126 621.6 0 126 211 137 621.6 0 137 212 147 621.6 0 147 213 164 621.6 0 164 214 327 621.6 0 327 215 484 621.6 0 484 216 405 621.6 0 405 217 262 621.6 0 262 218 203 621.6 0 203 219 181 621.6 0 181 220 136 621.6 0 136 221 139 621.6 0 139 222 160 621.6 0 160 223 204 621.6 0 204 224 235 621.6 0 235 225 460 621.6 0 460 226 1917 621.6 0 1917 227 5127 621.6 0 5127 228 3870 621.6 0 3870 229 4017 621.6 0 4017 230 684 621.6 0 684 231 902 621.6 0 902 232 679 621.6 0 679 233 467 621.6 0 467 234 124 621.6 0 124 235 113 621.6 0 113 236 132 621.6 0 132 237 168 621.6 0 168 238 216 621.6 0 216 239 213 621.6 0 213 240 188 621.6 0 188 241 113 621.6 0 113 242 114 621.6 0 114 243 96 621.6 0 96 244 136 621.6 0 136 245 134 621.6 0 134 246 154 621.6 0 154 247 146 621.6 0 146 248 194 621.6 0 194 249 193 621.6 0 193 250 158 621.6 0 158 251 135 621.6 0 135 252 143 621.6 0 143 253 124 621.6 0 124 254 131 621.6 0 131 255 127 621.6 0 127 256 146 621.6 0 146 257 165 621.6 0 165 258 152 621.6 0 152 259 164 621.6 0 164 260 178 621.6 0 178 261 238 621.6 0 238 262 207 621.6 0 207 263 242 621.6 0 242 264 789 621.6 0 789 265 1078 621.6 0 1078 266 1598 621.6 0 1598 267 915 621.6 0 915 268 867 621.6 0 867 269 257 621.6 0 257 270 253 621.6 0 253 271 212 621.6 0 212 272 166 621.6 0 166 273 221 621.6 0 221 274 273 621.6 0 273 275 318 621.6 0 318 276 322 621.6 0 322 277 164 621.6 0 164 278 183 621.6 0 183 279 226 621.6 0 226 280 257 621.6 0 257 281 245 621.6 0 245 282 219 621.6 0 219 283 164 621.6 0 164 284 206 621.6 0 206 285 158 621.6 0 158 286 185 621.6 0 185 287 221 621.6 0 221 288 183 621.6 0 183 289 184 621.6 0 184 290 187 621.6 0 187 291 678 621.6 0 678 292 606 621.6 0 606 293 641 621.6 0 641 294 214 621.6 0 214 295 111 621.6 0 111 296 127 621.6 0 127 297 128 621.6 0 128 298 128 621.6 0 128 299 143 621.6 0 143 300 150 621.6 0 150 301 203 621.6 0 203 Finished in 2.97 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 60,826 Reads with adapters: 14,587 (24.0%) Reads that were too short: 15 (0.0%) Reads written (passing filters): 14,572 (24.0%) Total basepairs processed: 14,398,061 bp Total written (filtered): 3,973,138 bp (27.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 14587 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 13 0.0 3 0 0 13 19 210 0.0 3 0 206 3 1 20 14256 0.0 4 13995 249 12 21 31 0.0 4 7 23 1 22 1 0.0 4 0 0 1 23 9 0.0 4 1 0 0 8 24 1 0.0 4 0 0 0 0 1 29 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 1 44 1 0.0 4 0 0 0 0 1 47 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 64 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 1 116 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 120 1 0.0 4 1 121 1 0.0 4 0 1 125 46 0.0 4 0 0 0 0 46 127 2 0.0 4 0 0 0 0 2 173 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 262 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.50 s (34 us/read; 1.75 M reads/minute). === Summary === Total reads processed: 14,572 Reads with adapters: 1,625 (11.2%) Reads written (passing filters): 14,572 (100.0%) Total basepairs processed: 3,973,138 bp Total written (filtered): 3,954,874 bp (99.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1625 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.6% C: 2.7% G: 94.5% T: 1.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 10 227.7 0 10 4 1 56.9 0 1 8 1 0.2 0 1 9 7 0.1 0 2 5 10 310 0.0 1 17 293 11 1289 0.0 1 7 1282 12 2 0.0 1 0 2 19 1 0.0 1 1 209 3 0.0 1 1 2 210 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.